Nucleic acid and corresponding protein entitled 121P1F1 useful in treatment and detection of cancer

ABSTRACT

A novel gene (designated 121P1F1) and its encoded protein, and variants thereof, are described wherein 121P1F1 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 121P1F1 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 121P1F1 gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 121P1F1 can be used in active or passive immunization.

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation-in-part of U.S. Ser. No. 09/779,250 filed Mar. 5, 2001. The content of that application is hereby incorporated by reference herein in its entirety.

STATEMENT OF RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH

[0002] Not applicable.

FIELD OF THE INVENTION

[0003] The invention described herein relates to a gene and its encoded protein, termed 121P1F1, expressed in certain cancers, and to diagnostic and therapeutic methods and compositions useful in the management of cancers that express 121P1F1.

BACKGROUND OF THE INVENTION

[0004] Cancer is the second leading cause of human death next to coronary disease. Worldwide, millions of people die from cancer every year. In the United States alone, as reported by the American Cancer Society, cancer causes the death of well over a half-million people annually, with over 1.2 million new cases diagnosed per year. While deaths from heart disease have been declining significantly, those resulting from cancer generally are on the rise. In the early part of the next century, cancer is predicted to become the leading cause of death.

[0005] Worldwide, several cancers stand out as the leading killers. In particular, carcinomas of the lung, prostate, breast, colon, pancreas, and ovary represent the primary causes of cancer death. These and virtually all other carcinomas share a common lethal feature. With very few exceptions, metastatic disease from a carcinoma is fatal. Moreover, even for those cancer patients who initially survive their primary cancers, common experience has shown that their lives are dramatically altered. Many cancer patients experience strong anxieties driven by the awareness of the potential for recurrence or treatment failure. Many cancer patients experience physical debilitations following treatment. Furthermore, many cancer patients experience a recurrence.

[0006] Worldwide, prostate cancer is the fourth most prevalent cancer in men. In North America and Northern Europe, it is by far the most common cancer in males and is the second leading cause of cancer death in men. In the United States alone, well over 30,000 men die annually of this disease—second only to lung cancer. Despite the magnitude of these figures, there is still no effective treatment for metastatic prostate cancer. Surgical prostatectomy, radiation therapy, hormone ablation therapy, surgical castration and chemotherapy continue to be the main treatment modalities. Unfortunately, these treatments are ineffective for many and are often associated with undesirable consequences.

[0007] On the diagnostic front, the lack of a prostate tumor marker that can accurately detect early-stage, localized tumors remains a significant limitation in the diagnosis and management of this disease. Although the serum prostate specific antigen (PSA) assay has been a very useful tool, however its specificity and general utility is widely regarded as lacking in several important respects.

[0008] Progress in identifying additional specific markers for prostate cancer has been improved by the generation of prostate cancer xenografts that can recapitulate different stages of the disease in mice. The LAPC (Los, Angeles Prostate Cancer) xenografts are prostate cancer xenografts that have survived passage in severe combined immune deficient (SCID) mice and have exhibited the capacity to mimic the transition from androgen dependence to androgen independence (Klein et al., 1997, Nat. Med. 3:402). More recently identified prostate cancer markers include PCTA-1 (Su et al., 1996, Proc. Natl. Acad. Sci. USA 93: 7252), prostate-specific membrane (PSM) antigen (Pinto et al., Clin Cancer Res 1996 Sep 2 (9): 1445-51), STEAP (Hubert, et al., Proc Natl Acad Sci USA. Dec. 7, 1999; 96(25): 14523-8) and prostate stem cell antigen (PSCA) (Rriter et a., 1998, Proc. Natl. Acad. Sci. USA 95: 1735).

[0009] While previously identified markers such as PSA, PSM, PCTA and PSCA have facilitated efforts to diagnose and treat prostate cancer, there is need for the identification of additional markers and therapeutic targets for prostate and related cancers in order to further improve diagnosis and therapy.

[0010] Renal cell carcinoma (RCC) accounts for approximately 3 percent of adult malignancies. Once adenomas reach a diameter of 2 to 3 cm, malignant potential exists. In the adult, the two principal malignant renal tumors are renal cell adenocarcinoma and transitional cell carcinoma of the renal pelvis or ureter. The incidence of renal cell adenocarcinoma is estimated at more than 29,000 cases in the United States, and more than 11,600 patients died of this disease in 1998. Transitional cell carcinoma is less frequent, with an incidence of approximately 500 cases per year in the United States.

[0011] Surgery has been the primary therapy for renal cell adenocarcinoma for many decades. Until recently, metastatic disease has been refractory to any systemic therapy. With recent developments in systemic therapies, particularly immunotherapies, metastatic renal cell carcinoma may be approached aggressively in appropriate patients with a possibility of durable responses. Nevertheless, there is a remaining need for effective therapies for these patients.

[0012] Of all new cases of cancer in the United States, bladder cancer represents approximately 5 percent in men (fifth most common neoplasm) and 3 percent in women (eighth most common neoplasm). The incidence is increasing slowly, concurrent with an increasing older population. In 1998, there was an estimated 54,500 cases, including 39,500 in men and 15,000 in women. The age-adjusted incidence in the United States is 32 per 100,000 for men and 8 per 100,000 in women. The historic male/female ratio of 3:1 may be decreasing related to smoking patterns in women. There were an estimated 11,000 deaths from bladder cancer in 1998 (7,800 in men and 3,900 in women). Bladder cancer incidence and mortality strongly increase with age and will be an increasing problem as the population becomes more elderly.

[0013] Most bladder cancers recur in the bladder. Bladder cancer is managed with a combination of transurethral resection of the bladder (TUR) and intravesical chemotherapy or immunotherapy. The multifocal and recurrent nature of bladder cancer points out the limitations of TUR. Most muscle-invasive cancers are not cured by TUR alone. Radical cystectomy and urinary diversion is the most effective means to eliminate the cancer but carry an undeniable impact on urinary and sexual function. There continues to be a significant need for treatment modalities that are beneficial for bladder cancer patients.

[0014] An estimated 130,200 cases of colorectal cancer occurred in 2000 in the United States, including 93,800 cases of colon cancer and 36,400 of rectal cancer. Colorectal cancers are the third most common cancers in men and women. Incidence rates declined significantly during 1992-1996 (−2.1% per year). Research suggests that these declines have been due to increased screening and polyp removal, preventing progression of polyps to invasive cancers. There were an estimated 56,300 deaths (47,700 from colon cancer, 8,600 from rectal cancer) in 2000, accounting for about 11% of all U.S. cancer deaths.

[0015] At present, surgery is the most common form of therapy for colorectal cancer, and for cancers that have not spread, it is frequently curative. Chemotherapy, or chemotherapy plus radiation, is given before or after surgery to most patients whose cancer has deeply perforated the bowel wall or has spread to the lymph nodes. A permanent colostomy (creation of an abdominal opening for elimination of body wastes) is occasionally needed for colon cancer and is infrequently required for rectal cancer. There continues to be a need fDr effective diagnostic and treatment modalities for colorectal cancer.

[0016] There were an estimated 164,100 new cases of lung and bronchial cancer in 2000, accounting for 14% of all U.S. cancer diagnoses. The incidence rate of lung and bronchial cancer is declining significantly in men, from a high of 86.5 per 100,000 in 1984 to 70.0 in 1996. In the 1990s, the rate of increase among women began to slow. In 1996, the incidence rate in women was 42.3 per 100,000.

[0017] Lung and bronchial cancer caused an estimated 156,900 deaths in 2000, accounting for 28% of all cancer deaths. During 1992-1996, mortality from lung cancer declined significantly among men (−1.7% per year) while rates for women were still significantly increasing (0.9% per year). Since 1987, more women have died each year of lung cancer than breast cancer, which, for over 40 years, was the major cause of cancer death in women. Decreasing lung cancer incidence and mortality rates most likely resulted from decreased smoking rates over the previous 30 years; however, decreasing smoking patterns among women lag behind those of men. Of concern, although the declines in adult tobacco use have slowed, tobacco use in youth is increasing again.

[0018] Treatment options for lung and bronchial cancer are determined by the type and stage of the cancer and include surgery, radiation therapy, and chemotherapy. For many localized cancers, surgery is usually the treatment of choice. Because the disease has usually spread by the time it is discovered, radiation therapy and chemotherapy are often needed in combination with surgery. Chemotherapy alone or combined with radiation is the treatment of choice for small cell lung cancer; on this regimen, a large percentage of patients experience remission, which in some cases is long lasting. There is however, an ongoing need for effective treatment and diagnostic approaches for lung and bronchial cancers.

[0019] An estimated 182,800 new invasive cases of breast cancer were expected to occur among women in the United States during 2000. Additionally, about 1,400 new cases of breast cancer were expected to be diagnosed in men in 2000. After increasing about 4% per year in the 1980s, breast cancer incidence rates in women have leveled off in the 1990s to about 110.6 cases per 100,000.

[0020] In the U.S. alone, there were an estimated 41,200 deaths (40,800 women, 400 men) in 2000 due to breast cancer. Breast cancer ranks second among cancer deaths in women. According to the most recent data, mortality rates declined significantly during 1992-1996 with the largest decreases in younger women, both white and black. These decreases were probably the result of earlier detection and improved treatment.

[0021] Taking into account the medical circumstances and the patient's preferences, treatment of breast cancer may involve lumpectomy (local removal of the tumor) and removal of the lymph nodes under the arm; mastectomy (surgical removal of the breast) and removal of the lymph nodes under the arm; radiation therapy; chemotherapy; or hormone therapy. Often, two or more methods are used in combination. Numerous studies have shown that, for early stage disease, long-term survival rates after lumpectomy plus radiotherapy are similar to survival rates after modified radical mastectomy. Significant advances in reconstruction techniques provide several options for breast reconstruction after mastectomy. Recently, such reconstruction has been done at the same time as the mastectomy.

[0022] Local excision of ductal carcinoma in situ (DCIS) with adequate amounts of surrounding normal breast tissue may prevent the local recurrence of the DCIS. Radiation to the breast and/or tamoxifen may reduce the chance of DCIS occurring in the remaining breast tissue. This is important because DCIS, if left untreated, may develop into invasive breast cancer. Nevertheless, there are serious side effects or sequelae to these treatments. There is, therefore, a need for efficacious breast cancer treatments.

[0023] There were an estimated 23,100 new cases of ovarian cancer in the United States in 2000. It accounts for 4% of all cancers among women and ranks second among gynecologic cancers. During 1992-0.1996, ovarian cancer incidence rates were significantly declining. Consequent to ovarian cancer, there were an estimated 14,000 deaths in 2000. Ovarian cancer causes more deaths than any other cancer of the female reproductive system.

[0024] Surgery, radiation therapy, and chemotherapy are treatment options for ovarian cancer. Surgery usually includes the removal of one or both ovaries, the fallopian tubes (salpingo-oophorectomy), and the uterus (hysterectomy). In some very early tumors, only the involved ovary will be removed, especially in young women who wish to have children. In advanced disease, an attempt is made to remove all intra-abdominal disease to enhance the effect of chemotherapy. There continues to be an important need for effective treatment options for ovarian cancer.

[0025] There were an estimated 28,300 new cases of pancreatic cancer in the United States in 2000. Over the past 20 years, rates of pancreatic cancer have declined in men. Rates among women have remained approximately constant but may be beginning to decline. Pancreatic cancer caused an estimated 28,200 deaths in 2000 in the United States. Over the past 20 years, there has been a slight but significant decrease in mortality rates among men (about −0.9% per year) while rates have increased slightly among women.

[0026] Surgery, radiation therapy, and chemotherapy are treatment options for pancreatic cancer. These treatment options can extend survival and/or relieve symptoms in many patients but are not likely to produce a cure for most. There is a significant need for additional therapeutic and diagnostic options for pancreatic cancer.

SUMMARY OF THE INVENTION

[0027] The present invention relates to a gene, designated 121P1F1, that has now been found to be over-expressed in the cancer(s) listed in Table I. Northern blot expression analysis of 121P1F1 gene expression in normal tissues shows a restricted expression pattern in adult tissues. The nucleotide (FIG. 2) and amino acid (FIG. 2, and FIG. 3) sequences of 121P1F1 are provided. The tissue-related profile of 121P1F1 in normal adult tissues, combined with the over-expression observed in the tumors listed in Table I, shows that 121P1F1 is aberrantly over-expressed in at least some cancers, and thus serves as a useful diagnostic, prophylactic, prognostic, and/or therapeutic target for cancers of the tissue(s) such as those listed in Table I.

[0028] The invention provides polynucleotides corresponding or complementary to all or part of the 121P1F1 genes, mRNAs, and/or coding sequences, preferably in isolated form, including polynucleotides encoding 121P1F1-related proteins and fragments of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, or more than 25 contiguous amino acids; at least 30, 35, 40, 45, 50, 55, 60, 65, 70, 80, 85, 90, 95, 100 or more than 100 contiguous amino acids of a 121P1F1-related protein, as well as the peptides/proteins themselves; DNA, RNA, DNA/RNA hybrids, and related molecules, polynucleotides or oligonucleotides complementary or having at least a 90% homology to the 121P1F1 genes or mRNA sequences or parts thereof, and polynucleotides or oligonucleotides that hybridize to the 121P1F1 genes, mRNAs, or to 121P1F1-encoding polynucleotides. Also provided are means for isolating cDNAs and the genes encoding 121P1F1. Recombinant DNA molecules containing 121P1F1 polynucleotides, cells transformed or transduced with such molecules, and host-vector systems for the expression of 121P1F1 gene products are also provided. The invention further provides antibodies that bind to 121P1F1 proteins and polypeptide fragments thereof, including polyclonal and monoclonal antibodies, murine and other mammalian antibodies, chimeric antibodies, humanized and fully human antibodies, and antibodies labeled with a detectable marker or therapeutic agent. In certain embodiments there is a proviso that the entire nucleic acid sequence of FIG. 2 is not encoded and/or the entire amino acid sequence of FIG. 2 is not prepared. In certain embodiments, the entire nucleic acid sequence of FIG. 2 is encoded and/or the entire amino acid sequence of FIG. 2 is prepared, either of which are in respective human unit dose forms.

[0029] The invention further provides methods for detecting the presence and status of 121P1F1 polynucleolides and proteins in various biological samples, as well as methods for identifying cells that express 121P1F1. A typical embodiment of this invention provides methods for monitoring 121P1F1 gene products in a tissue or hematology sample having or suspected of having some form of growth dysregulation such as cancer.

[0030] The invention further provides various immunogenic or therapeutic compositions and strategies for treating cancers that express 121P1F1 such as cancers of tissues listed in Table I, including therapies aimed at inhibiting the transcription, translation, processing or function of 121P1F1 as well as cancer vaccines. In one aspect, the invention provides compositions, and methods comprising them, for treating a cancer that expresses 121P1F1 in a human subject wherein the composition comprises a carrier suitable for human use and a human unit dose of one or more than one agent that inhibits the production or function of 121P1F1. Preferably, the carrier is a uniquely human carrier. In another aspect of the invention, the agent is a moiety that is immunoreactive with 121P1F1 protein. Non-limiting examples of such moieties include, but are not limited to, antibodies (such as single chain, monoclonal, polyclonal, humanized, chimeric, or human antibodies), functional equivalents thereof (whether naturally occurring or synthetic), and combinations thereof. The antibodies can be conjugated to a diagnostic or therapeutic moiety. In another aspect, the agent is a small molecule as defined herein.

[0031] In another aspect, the agent comprises one or more than one peptide which comprises a cytotoxic T lymphocyte (CTL) epitope that binds an HLA class I molecule in a human to elicit a CTL response to 121P1F1 and/or one or more than one peptide which comprises a helper T lymphocyte (HTL) epitope which binds an HLA class II molecule in a human to elicit an HTL response. The peptides of the invention may be on the same or on one or more separate polypeptide molecules. In a further aspect of the invention, the agent comprises one or more than one nucleic acid molecule that expresses one or more than one of the CTL or HTL response stimulating peptides as described above. In yet another aspect of the invention, the one or more than one nucleic acid molecule may express a moiety that is immunologically reactive with 121P1F1 as described above. The one or more than one nucleic acid molecule may also be, or encodes, a molecule that inhibits production of 121P1F1. Non-limiting examples of such molecules include, but are not limited to, those complementary to a nucleotide sequence essential for production of 121P1F1 (e.g. antisense sequences or molecules that form a triple helix with a nucleotide double helix essential for 121P1F1 production) or a ribozyme effective to lyse 121P1F1 mRNA.

BRIEF DESCRIPTION OF THE FIGURES

[0032]FIG. 1. The 121P1F1 SSH sequence of 254 nucleotides.

[0033]FIG. 2. The cDNA (SEQ ID. NO.: ______) and amino acid sequence (SEQ ID. NO.: ______) of 121P1F1 is shown in FIG. 2A. The start methionine is underlined. The open reading frame extends from nucleic acid 82-699 including the stop codon. The nucleic acid and amino acid sequence of 121P1F1 variant 1A (SEQ ID. NO.: ______) is shown in FIG. 2B, the codon for the start methionine is underlined. The open reading frame for variant 1A extends from nucleic acid 82 to 462 including the stop codon. The nucleic acid, and amino acid sequence of 121P1F1 variant 1B (SEQ ID. NO.: ______) is shown in FIG. 2C, the codon for the start methionine is underlined. The open reading frame for variant 1B extends from nucleic acid. 501-860 including the stop codon. The nucleic acid and amino acid sequence of 121P1F1 variant 2 (SEQ ID. NO.: ______) is shown in FIG. 2D, the codon for the start methionine is underlined. The open reading frame for variant 2 extends from nucleic acid 82-450 including the stop codon. The nucleic acid and amino acid sequence of 121P1F1 variant 3 (SEQ ID. NO.: ______) is shown in FIG. 2E, the codon for the start methionine is underlined. The open reading frame for variant 3 extends from nucleic acid 82-654 including the stop codon. The nucleic acid and amino acid sequence of 121P1F1 variant 4 (SEQ ID. NO.: ______) is shown in FIG. 2F, the codon for the start methionine is underlined. The open reading frame for variant 4 extends from nucleic acid 281-853 including the stop codon.

[0034]FIG. 3. Amino acid sequence of 121P1F1 (SEQ ID. NO.: ______) is shown in FIG. 3A; it has 205 amino acids. The amino acid sequence of 121P1F1 variant 1A (SEQ ID. NO.: ______) is shown in FIG. 3B; it has 126 amino acids. The amino acid sequence of 121P1F1 variant 1B (SEQ ID. NO.: ______) is shown in FIG. 3C, the 121P1F1 variant 1B protein has 119 amino acids. The amino acid sequence of 121P1F1 variant 2 (SEQ ID. NO.: ______) is shown in FIG. 3D, the 121P1F1 variant 2 protein has 122 amino acids. The amino acid sequence of 121P1F1 variant 3 (SEQ ID. NO.: ______) is shown in FIG. 3E, the 121P1F1 variant 3 protein has 190 amino acids. The amino acid sequence of 121P1F1 variant 4 (SEQ ID. NO.: ______) is shown in FIG. 3F, the 121P1F1 variant 4 protein has 190 amino acids.

[0035]FIG. 4. A. The amino acid alignments of 121P1F1 protein and variants 1A, 1B, 2, and 3. B. The amino acid alignments of 121P1F1 protein and variants 4 and 1A. C. Alignment with human protein GAJ. D. Alignment with closest mouse homolog. E. Alignment with hypothetical yeast protein.

[0036]FIG. 5. Hydrophilicity amino acid profile of A) 121P1F1 and B) 121P1F1 var1A determined by computer algorithm sequence analysis using the method of Hopp and Woods (Hopp T. P., Woods K. R., 1981. Proc. Natl. Acad. Sci. U.S.A. 78:3824-3828) accessed on the Protscale website (www.expasy.ch/cgi-bin/protscaile.pl) through the ExPasy molecular biology server.

[0037]FIG. 6. Hydropathicity amino acid profile of A) 121P1F1 and B) 121P1F1 var1A determined by computer algorithm sequence analysis using the method of Kyte and Doolittle (Kyte J., Doolittle R. F., 1982. J. Mol. Biol. 157:105-132) accessed on the ProtScale website (www.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server.

[0038]FIG. 7. Percent accessible residues amino acid profile of A) 121P1F1 and B) 121P1F1 var1A determined by computer algorithm sequence analysis using the method of Janin (Janin J., 1979 Nature 277:491-492) accessed on the ProtScale website (www.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server.

[0039]FIG. 8. Average flexibility amino acid profile of A) 121P1F1 and B) 121P1F1 var1A determined by computer algorithm sequence analysis using the method of Bhaskaran and Ponnuswamy (Bhaskaran R., and Ponnuswamy P. K., 1988. Int. J. Pept. Protein Res. 32:242-255) accessed on the ProtScale website (www.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server.

[0040]FIG. 9. Beta-turn amino acid profile of A) 121P1F1 and B) 121P1F1 var1A determined by computer algorithm sequence analysis using the method of Deleage and Roux (Deleage, G., Roux B. 1987 Protein Engineering 1:289-294) accessed on the ProtScale website (www.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server.

[0041]FIG. 10. Nucleotide splice variants of 121P1F1.

[0042]FIG. 11. Protein splice variants of 121P1F1.

[0043]FIG. 12: Specific recognition of 121P1F1 antigen by anti-121P1F1 polyclonal antibody. The indicated dilutions of anti-121P1F1 polyclonal antibody serum or pre-immune serum was used to probe a blot containing GST-121P1F1 cleavage antigen. Reactivity was visualized by incubation with goat anti-rabbit HRP-conjugated secondary antibody and development by enhanced chemiluminescence and exposure to autoradiography film.

[0044]FIG. 13: Expression of 121P1F1 in various cancer cells. Anti-121P1F1 polyclonal antibody was used to carry out Western blot analysis of 121P1F1 expression in cell lysates from the indicated cancer cell lines and Myc His tagged 121P1F1 expressed in 293T cells. Seen is specific anti-121P1F1 reactive bands in each of the cancer cell lines indicative of endogenous 121P1F1 expression and possibly recognition of 121P1F1 splice variants of different molecular weights.

[0045]FIG. 14: Expression of 121P1F1 in 293T cells. Cell lysates of vector or pcDNA 3.1-Myc His 121P1F1 transfected 293T cells were subjected to Western analysis with anti-His polyclonal antibody (Santa Cruz Biotechnology). Seen is a 35 kD band representing expression of 121P1F1 Myc His-tagged protein.

[0046]FIG. 15. Androgen regulation of 121P1F1 in vivo. Male mice were injected with LAPC-9AD tumor cells. When tumor reached a palpable size (0.3-0.5 cm in diameter), mice were castrated and tumors harvested at different time points following castration. RNA was isolated from the xenograft tissues. Northern blots with 10 μg of total RNA/lane were probed with the 121P1F1 SSH fragment. Size standards in kilobases (kb) are indicated on the side. Results show expression of 121P1F1 is slightly downregulated 7 days after castration. The protein TMPRSS2 was used as a positive control. A picture of the ethidium-bromide staining of the RNA gel is also presented (lowest panel).

[0047]FIG. 16: Secondary structure prediction for 121P1F1 (FIG. 16A) and variant 1a (FIG. 16B). The secondary structure of 121P1F1 and variant 1a proteins were predicted using the HNN—Hierarchical Neural Network method (Guerrneur, 1997, Web URL pbil.ibcp.fr/cgi-bin/npsaau.tomat.pl?page=npsa_nn.html), accessed from the ExPasy molecular biology server (Web URL www.expasy.ch/tools/). This method predicts the presence and location of alpha helices, extended strands, and random coils from the primary protein sequence. The percent of the protein in a given secondary structure is also given.

[0048]FIG. 17. RT-PCR analysis of 121P1F1 expression. First strand cDNA was prepared (A) from 8 human normal tissues, and (B) from vital pool 1 (VP1: liver, lung and kidney), vital pool 2 (VP2, pancreas, spleen and stomach), LAPC xenograft pool (XP; LAPC-4AD, LAPC-4AI, LAPC-9AD and LAPC-9AI), normal prostate (NP), prostate cancer pool, bladder cancer pool, kidney cancer pool, colon cancer pool and lung cancer pool. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 121P1F1, was performed at 25 and 30 cycles of amplification.

[0049]FIG. 18. Expression of 121P1F1 in normal human tissues by Northern blot analysis. Two multiple tissue northern blots (Clontech) with 2 μg of mRNA/lane, were probed with the 121P1F1 SSH fragment. Size standards in kilobases (kb) are indicated on the side. The results show exclusive expression of an approximately 1.2 kb 121P1F1 transcript in testis and to a lower level in thymus.

[0050]FIG. 19. Expression of 121P1F1 in cancer cell lines. RNA was extracted from a number of cancer cell lines. Northern blots with 10 μg of total RNA/lane were probed with the 121P1F1 SSH fragment. Size standards in kilobases (kb) are indicated on the side.

[0051]FIG. 20. Expression of 121P1F1 in prostate cancer patient samples. RNA was extracted from the prostate tumors (T) and their normal adjacent tissue (N) derived from prostate cancer patients. Tumors of patients 1, 2 and 3 have a Gleason score of 6. Tumors of patients 4, 5 and 6 have a Gleason score of 7. Tumors of patients 7, 8 and 9 have a Gleason score of 9. Northern blots with 10 μg of total RNA/lane were probed with the 121P1F1 SSH fragment. Size standards in kilobases (kb) are indicated on the side.

[0052]FIG. 21. Expression of 121P1F1 in human patient cancer specimens and cancer cell lines. Expression of 121P1F1 was assayed in a panel of human cancers (T) and their respective matched normal tissues (N) on RNA dot blots. 121P1F1 expression was seen in kidney, breast, cervix, and stomach cancers. 121P1F1 was also found to be highly expressed in a panel of cancer cell lines in the following cancer cell lines; HeLa, Daudi, K562, HL-60, G361, A549, MOLT-4, SW480, and Raji.

[0053]FIG. 22. Androgen regulation of 121P1F1 in vitro. LAPC-4² cells were grown in charcoal-stripped medium and stimulated with the synthetic androgen mibolerone, for either 14 or 24 hours. Northern blot was performed with 10 μg of total RNA for each sample, and probed with the 121P1F1 SSH fragment. A picture of the ethidium-bromide staining of the RNA gel is also presented (lowest panel). Hybridization of the same northern blot with the androgen-dependent gene TMPRSS2 confirms the quality of the the androgen deprivation. The results show that the expression of 121P1F1 goes down in absence of normal serum, and is modulated in presence of mibolerone, 24 hours after stimulation.

DETAILED DESCRIPTION OF THE INVENTION

[0054] Outline of Sections

[0055] I.) Definitions

[0056] II.) 121P1F1 Polynucleotides

[0057] II.A.) Uses of 121P1F1 Polynucleotides

[0058] II.A.1.) Monitoring of Genetic Abnormalities

[0059] II.A.2.) Antisense Embodiments

[0060] II.A.3.) Primers and Primer Pairs

[0061] II.A.4.) Isolation of 121P1F1-Encoding Nucleic Acid Molecules

[0062] II.A.5.) Recombinant Nucleic Acid Molecules and Host-Vector Systems

[0063] III.) 121P1F1-related Proteins

[0064] III.A.) Motif-bearing Protein Embodiments

[0065] III.B.) Expression of 121P1F1-related Proteins

[0066] III.C.) Modifications of 121P1F1-related Proteins

[0067] III.D.) Uses of 121P1F1-related Proteins

[0068] IV.) 121P1F1 Antibodies

[0069] V.) 121P1F1 Cellular Immune Responses

[0070] VI.) 121P1F1 Transgenic Animals

[0071] VII.) Methods for the Detection of 121P1F1

[0072] VIII.) Methods for Monitoring the Status of 121P1F1-related Genes and Their Products

[0073] IX.) Identification of Molecules That Interact With 121P1F1

[0074] X.) Therapeutic Methods and Compositions

[0075] X.A.) Anti-Cancer Vaccines

[0076] X.B.) 121P1F1 as a Target for Antibody-Based Therapy

[0077] X.C.) 121P1F1 as a Target for Cellular Immune Responses

[0078] X.C.1. Minigene Vaccines

[0079] X.C.2. Combinations of CTL Peptides with Helper Peptides

[0080] X.C.3. Combinations of CTL Peptides with T Cell Priming Agents

[0081] X.C.4. Vaccine Compositions Comprising DC Pulsed with CTL and/or HTL Peptides

[0082] X.D.) Adoptive Immunotherapy

[0083] X.E.) Administration of Vaccines for Therapeutic or Prophylactic Purposes

[0084] XI.) Diagnostic and Prognostic Embodiments of 121P1F1.

[0085] XII.) Inhibition of 121P1F1 Protein Function

[0086] XII.A.) Inhibition of 121P1F1 With Intracellular Antibodies

[0087] XII.B.) Inhibition of 121P1F1 with Recombinant Proteins

[0088] XII.C.) Inhibition of 121P1F1 Transcription or Translation

[0089] XII.D.) General Considerations for Therapeutic Strategies

[0090] XIII.) KITS

[0091] I) Definitions:

[0092] Unless otherwise defined, all terms of art, notations and other scientific terms or terminology used herein are intended to have the meanings commonly understood by those of skill in the art to which this invention pertains. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such definitions herein should not necessarily be construed to represent a substantial difference over what is generally understood in the art. Many of the techniques and procedures described or referenced herein are well understood and commonly employed using conventional methodology by those skilled in the art, such as, for example, the widely utilized molecular cloning methodologies described in Sambrook et al., Molecular Cloning: A Laboratory Manual 2nd. edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. As appropriate, procedures involving the use of commercially available kits and reagents are generally carried out in accordance with manufacturer defined protocols and/or parameters unless otherwise noted.

[0093] The terms “advanced prostate cancer”, “locally advanced prostate cancer”, “advanced disease” and “locally advanced disease” mean prostate cancers that have extended through the prostate capsule, and are meant to include stage C disease under the American Urological Association (AUA) system, stage C1-C2 disease under the Whitmore-Jewett system, and stage T3-T4 and N+ disease under the TNM (tumor, node, metastasis) system. In general, surgery is not recommended for patients with locally advanced disease, and these patients have substantially less favorable outcomes compared to patients having clinically localized (organ-confined) prostate cancer. Locally advanced disease is clinically identified by palpable evidence of induration beyond the lateral border of the prostate, or asymmetry or induration above the prostate base. Locally advanced prostate cancer is presently diagnosed pathologically following radical prostatectomy if the tumor invades or penetrates the prostatic capsule, extends into the surgical margin, or invades the seminal vesicles.

[0094] “Altering the native glycosylation pattern” is intended for purposes herein to mean deleting one or more carbohydrate moieties found in native sequence 121P1F1 (either by removing the underlying glycosylation site or by deleting the glycosylation by chemical and/or enzymatic means), and/or adding one or more glycosylation sites that are not present in the native sequence 121P1F1. In addition, the phrase includes qualitative changes in the glycosylation of the native proteins, involving a change in the nature and proportions, of the various carbohydrate moieties present.

[0095] The term “analog” refers to a molecule which is structurally similar or shares similar or corresponding attributes with another molecule (e.g. a 121P1F1-related protein). For example an analog of a 121P1F1 protein can be specifically bound by an antibody or T cell that specifically binds to 121P1F1.

[0096] The term “antibody” is used in the broadest sense. Therefore an “antibody” can be naturally occurring or man-made such as monoclonal antibodies produced by conventional hybridoma technology. Anti-121P1F1 antibodies comprise monoclonal and polyclonal antibodies as well as fragments containing the antigen-binding domain and/or one or more complementarity determining regions of these antibodies.

[0097] An “antibody fragment” is defined as at least a portion of the variable region of the immunoglobulin molecule that binds to its target, i.e., the antigen-binding region. In one embodiment it specifically covers single anti-121P1F1 antibodies and clones thereof (including agonist, antagonist and neutralizing, antibodies) and anti-121P1F1 antibody compositions with polyepitopic specificity.

[0098] The term “codon optimized sequences” refers to nucleotide sequences that have been optimized for a particular host species by replacing any codons having a usage frequency of less than about 20%. Nucleotide sequences that have been optimized for expression in a given host species by elimination of spurious polyadenylation sequences, elimination of exon/intron splicing signals, elimination of transposon-like repeats and/or optimization of GC content in addition to codon optimization are refer-red to herein as an “expression enhanced sequences.”

[0099] The term “cytotoxic agent” refers to a substance that inhibits or prevents the expression activity of cells, function of cells and/or causes destruction of cells. The term is intended to include radioactive isotopes chemotherapeutic agents, and toxins such as small molecule toxins or enzymatically active toxins of bacterial, fungal, plant or animal origin, including fragments and/or variants thereof. Examples of cytotoxic agents include, but are not limited to maytansinoids, yttrium, bismuth, ricin, ricin A-chain, doxorubicin, daunorubicin, taxol, ethidium bromide, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicine, dihydroxy anthracin dione, actinomycin, diphtheria toxin, Pseudomonas exotoxin (PE) A, PE40, abrin, abrin A chain, modeccin A chain, alpha-sarcin, gelonin, mitogellin, retstrictocin, phenomycin, enomycin, curicin, crotin, calicheamicin, sapaonaria officinalis inhibitor, and glucocorticoid and other chemotherapeutic agents, as well as radioisotopes such as At²¹¹, I¹³¹, I¹²⁵, Y⁹⁰, Re¹⁸⁶, Re¹⁸⁸, Sm¹⁵³, Bi²¹², P³² and radioactive isotopes of Lu. Antibodies may also be conjugated to an anti-cancer pro-drug activating enzyme capable of converting the pro-drug to its active form.

[0100] The term “homolog” refers to a molecule which exhibits homology to another molecule, by for example, having sequences of chemical residues that are the same or similar at corresponding positions.

[0101] “Human Leukocyte Antigen” or “HLA” is a human class I or class II Major Histocompatibility Complex (TVHC) protein (see, eg., Stites, et al., IMMUNOLOGY, 8^(TH) ED., Lange Publishing, Los Altos, Calif. (1994).

[0102] The terms “hybridize”, “hybridizing”, “hybridizes” and the like, used in the context of polynucleotides, are meant to refer to conventional hybridization conditions, preferably such as hybridization in 50% formamide/6×SSC/0.1% SDS/100 μg/ml ssDNA, in which temperatures for hybridization are above 37 degrees C. and temperatures for washing in 0.1×SSC/0.1% SDS are above 55 degrees C.

[0103] The phrases “isolated” or “biologically pure” refer to material which is substantially or essentially free from components which normally accompany the material as it is found in its native state. Thus, isolated peptides in accordance with the invention preferably do not contain materials normally associated with the peptides in their in situ environment. For example, a polynucleotide is said to be “isolated” when it is substantially separated from contaminant polynucleotides that correspond or are complementary to genes other than the 121P1F1 genes or that encode polypeptides other than 121P1F1 gene product or fragments thereof. A skilled artisan can readily employ nucleic acid isolation procedures to obtain an isolated 121P1F1 polynucleotide. A protein is said to be “isolated,” for example, when physical, mechanical or chemical methods arex employed to remove the 121P1F1 proteins from cellular constituents that are normally associated with the protein. A skilled artisan can readily employ standard purification methods to obtain an isolated 121P1F1 protein. Alternatively, an isolated protein can be prepared by chemical means.

[0104] The term “mammal” refers to any organism classified as a mammal, including mice, rats, rabbits, dogs, cats, cows, horses and humans. In one embodiment of the invention, the mammal is a mouse. In another embodiment of the invention, the mammal is a human.

[0105] The terms “metastatic prostate cancer” and “metastatic disease” mean prostate cancers that have spread to regional lymph nodes or to distant sites, and are meant to include stage D disease under the AUA system and stage TxNxM+under the TNM system. As is the case with locally advanced prostate cancer, surgery is generally not indicated for patients with metastatic disease, and hormonal (androgen ablation) therapy is a preferred treatment modality. Patients with metastatic prostate cancer eventually develop an androgen-refractory state within 12 to 18 months of treatment initiation. Approximately half of these androgen-refractory patients die within 6 months after developing that status. The most common site for prostate cancer metastasis is bone. Prostate cancer bone metastases are often osteoblastic rather than osteolytic (i.e., resulting in net bone formation). Bone metastases are found most frequently in the spine, followed by the femur, pelvis, rib cage, skull and humerus. Other common sites for metastasis include lymph nodes, lung, liver and brain. Metastatic prostate cancer is typically diagnosed by open or laparoscopic pelvic lymphadenectomy, whole body radionuclide scans, skeletal radiography, and/or bone lesion biopsy.

[0106] The term “monoclonal antibody” refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the antibodies comprising the population are identical except for possible naturally occurring mutations that are present in minor amounts.

[0107] A “motif”, as in biological motif of an 121P1F1-related protein, refers to any pattern of amino acids forming part of the primary sequence of a protein, that is associated with a particular function (e.g. protein-protein interaction, protein-DNA interaction, etc) or modification (e.g. that is phosphorylated, glycosylated or amidated), or localization (e.g. secretory sequence, nuclear localization sequence, etc.) or a sequence that is correlated with being immunogenic, either humorally or cellularly. A motif can be either contiguous or capable of being aligned to certain positions that are generally correlated with a certain function or property. In the context of HLA motifs, “motif” refers to the pattern of residues in a peptide of defined length, usually a peptide of from about 8 to about 13 amino acids for a class I HLA motif and from about 6 to about 25 amino acids for a class II HLA motif, which is recognized by a particular HLA molecule. Peptide motifs for HLA binding are typically different for each protein encoded by each human HLA allele and differ in the pattern of the primary and secondary anchor residues.

[0108] A “pharmaceutical excipient” comprises a material such as an adjuvant, a carrier, pH-adjusting and buffering agents, tonicity adjusting agents, wetting agents, preservative, and the like.

[0109] “Pharmaceutically acceptable” refers to a non-toxic, inert, and/or composition that is physiologically compatible with humans or other mammals.

[0110] The term “polynucleotide” means a polymeric form of nucleotides of at least 10 bases or base pairs in length, either ribonucleotides or deoxynucleotides or a modified form of either type of nucleotide, and is meant to include single and double stranded forms of DNA and/or RNA. In the art, this term if often used interchangeably with “oligonucleotide”. A polynucleotide can comprise a nucleotide sequence disclosed herein wherein thymidine (T), as shown for example in FIG. 2, can also be uracil (U); this definition pertains to the differences between the chemical structures of DNA and RNA, in particular the observation that one of the four major bases in RNA is uracil (U) instead of thymidine (T).

[0111] The term “polypeptide” means a polymer of at least about 4, 5, 6, 7, or 8 amino acids. Throughout the specification, standard three letter or single letter designations for amino acids are used. In the art, this term is often used interchangeably with “peptide” or “protein”.

[0112] An HLA “primary anchor residue” is an amino acid at a specific position along a peptide sequence which is understood to provide a contact point between the immunogenic peptide and the HLA molecule, One to three, usually two, primary anchor residues within a peptide of defined length generally defines a “motif” for an immunogenic peptide. These residues are understood to fit in close contact with peptide binding groove of an HLA molecule, with their side chains buried in specific pockets of the binding groove. In one embodiment, for example, the primary anchor residues for an HLA class I molecule are located at position 2 (from the amino terminal position) and at the carboxyl terminal position of a 8, 9, 10, 11, or 12 residue peptide epitope in accordance with the invention. In another embodiment, for example, the primary anchor residues of a peptide that will bind an HLA class II molecule are spaced relative to each other, rather than to the termini of a peptide, where the peptide is generally of at least 9 amino acids in length. The primary anchor positions for each motif and supermotif are set forth in Table IV. For example, analog peptides can be created by altering the presence or absence of particular residues in the primary and/or secondary anchor positions shown in Table IV. Such analogs are used to modulate the binding affinity and/or population coverage of a peptide comprising a particular HLA motif or supermotif.

[0113] A “recombinant” DNA or RNA molecule is a DNA or RNA molecule that has been subjected to molecular manipulation in vitro.

[0114] Non-limiting examples of small molecules include compounds that bind or interact with 121P1F1, ligands including hormones, neuropeptides, chemokines, odorants, phospholipids, and functional equivalents thereof that bind and preferably inhibit 121P1F1 protein function. Such non-limiting small molecules preferably have a molecular weight of less than about 10 kDa, more preferably below about 9, about 8, about 7, about 6, about 5 or about 4 kDa. In certain embodiments, small molecules physically associate with, or bind, 121P1F1 protein; are not found in naturally occurring metabolic pathways; and/or are more soluble in aqueous than non-aqueous solutions

[0115] “Stringency” of hybridization reactions is readily determinable by one of ordinary skill in the art, and generally is an empirical calculation dependent upon probe length, washing temperature, and salt concentration. In general, longer probes require higher temperatures for proper annealing, while shorter probes need lower temperatures. Hybridization generally depends on the ability of denatured nucleic acid sequences to reanneal when complementary strands are present in an environment below their melting temperature. The higher the degree of desired homology between the probe and hybridizable sequence, the higher the relative temperature that can be used. As a result, it follows that higher relative temperatures would tend to make the reaction conditions more stringent, while lower temperatures less so. For additional details and explanation of stringency of hybridization reactions, see Ausubel et al., Current Protocols in Molecular Biology, Wiley Interscience Publishers, (1995).

[0116] “Stringent conditions” or “high stringency conditions”, as defined herein, are identified by, but not limited to, those that: (1) employ low ionic strength and high temperature for washing, for example 0.015 M sodium chloride/0.0015 M sodium citrate/0.1% sodium dodecyl sulfate at 50° C.; (2) employ during hybridization a denaturing agent, such as formamide, for example, 50% (v/v) formamide with 0.1% bovine serum albumin/0.1% Ficoll/0.1% polyvinylpyrrolidone/50 mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42° C.; or (3) employ 50% formamide, 5×SSC (0.75 M NaCl, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5× Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC (sodium chloride/sodium. citrate) and 50% formamide at 55° C., followed by a high-stringency wash consisting of 0.1×SSC containing EDTA at 55° C. “Moderately stringent conditions” are described by, but not limited to, those in Sambrook et al., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Press, 1989, and include the use of washing solution and hybridization conditions (e.g., temperature, ionic strength and %SDS) less stringent than those described above. An example of moderately stringent conditions is overnight incubation at 37° C. in a solution comprising: 20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 mg/mL denatured sheared salmon sperm DNA, followed by washing the filters in 1×SSC at about 37-50° C. The skilled artisan will recognize how to adjust the temperature, ionic strength, etc. as necessary to accommodate factors such as probe length and the like.

[0117] An HLA “supermotif” is a peptide binding specificity shared by HLA molecules encoded by two or more HLA alleles.

[0118] As used herein “to treat” or “therapeutic” and grammatically related terms, refer to any improvement of any consequence of disease, such as prolonged survival, less morbidity, and/or a lessening of side effects which are the byproducts of an alternative therapeutic modality; full eradication of disease is not required.

[0119] A “transgenic animal” (e.g., a mouse or rat) is an animal having cells that contain a transgene, which transgene was introduced into the animal or an ancestor of the animal at a prenatal, e.g., an embryonic stage. A “transgene” is a DNA that is integrated into the genome of a cell from which a transgenic animal develops.

[0120] As used herein, an HLA or cellular immune response “vaccine” is a composition that contains or encodes one or more peptides of the invention. There are numerous embodiments of such vaccines, such as a cocktail of one or more individual peptides; one or more peptides of the invention comprised by a polyepitopic peptide; or nucleic acids that encode such individual peptides or polypeptides, e.g., a minigene that encodes a polyepitopic peptide. The “one or more peptides” can include any whole unit integer from 1-150 or more, e.g., at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, or 150 or more peptides of the invention. The peptides or polypeptides can optionally be modified, such as by lipidation, addition of targeting or other sequences. HLA class I peptides of the invention can be admixed with, or linked to, HLA class II peptides, to facilitate activation of both cytotoxic T lymphocytes and helper T lymphocytes. HLA vaccines can also comprise peptide-pulsed antigen presenting cells, e.g., dendritic cells.

[0121] The term “variant” refers to a molecule that exhibits a variation from a described type or norm, such as a protein that has one or more different amino acid residues in the corresponding position(s) of a specifically described protein (e.g. the 121P1F1 protein shown in FIG. 2 or FIG. 3. An analog is an example of a variant protein. Splice isoforms and single nucleotides polymorphisms (SNPs) are further examples of variants.

[0122] The “121P1F1-related proteins” of the invention include those specifically identified herein, as well as allelic variants, conservative substitution variants, analogs and homologs that can be isolated/generated and characterized without undue experimentation following the methods outlined herein or readily available in the art. Fusion proteins that combine parts of different 121P1F1 proteins or fragments thereof, as well as fusion proteins of a 121P1F1 protein and a heterologous polypeptide are also included. Such 121P1F1 proteins are collectively referred to as the 121P1F1-related proteins, the proteins of the invention, or 121P1F1. The term “121P1F1-related protein” refers to a polypeptide fragment or an 121P1F1 protein sequence of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, or more than 25 amino acids; or, at least 30, 35, 40, 45, 50, 55, 60, 65, 70, 80, 85, 90, 95, 100 or more than 100 amino acids.

[0123] II.) 121P1F1 Polynucleotides

[0124] One aspect of the invention provides polynucleotides corresponding or complementary to all or part of an 121P1F1 gene, mRNA, and/or coding sequence, preferably in isolated form, including polynucleotides encoding an 121P1F1-related protein and fragments thereof, DNA, RNA, DNA/RNA hybrid, and related molecules, polynucleotides or oligonucleotides complementary to an 121P1F1 gene or mRNA sequence or a part thereof, and polynucleotides or oligonucleotides that hybridize to an 121P1F1 gene, mRNA, or to an 121P1F1 encoding polynucleotide (collectively, “121P1F1 polynucleotides”). In all instances when referred to in this section, T can also be U in FIG. 2.

[0125] Embodiments of a 121P1F1 polynucleotide include: a 121P1F1 polynucleotide having the sequence shown in FIG. 2, the nucleotide sequence of 121P1F1 as shown in FIG. 2 wherein T is U; at least 10 contiguous nucleotides of a polynucleotide having the sequence as shown in FIG. 2; or, at least 10 contiguous nucleotides of a polynucleotide having the sequence as shown in FIG. 2 where T is U. For example, embodiments of 121P1F1 nucleotides comprise, without limitation:

[0126] (I) a polynucleotide comprising, consisting essentially of, or consisting of a sequence as shown in FIG. 2 (SEQ ID NO: ______), wherein T can also be U;

[0127] (II) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in FIG. 2A (SEQ ID NO: ______), from nucleotide residue number 82 through nucleotide residue number 696, followed by a stop codon, wherein T can also be U;

[0128] (III) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in FIG. 2B (SEQ ID NO: ______), from nucleotide residue number 82 through nucleotide residue number 459, followed by a stop codon, wherein T can also be U;

[0129] (IV) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in FIG. 2C (SEQ ID NO: ______), from nucleotide residue number 501 through nucleotide residue number 857, followed by a stop codon, wherein T can also be U;

[0130] (V) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in FIG. 2D (SEQ ID NO: ______), from nucleotide residue number 82 through nucleotide residue number 447, followed by a stop codon, wherein T can also be U;

[0131] (VI) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in FIG. 2E (SEQ ID NO: ______), from nucleotide residue number 82 through nucleotide residue number 651, followed by a stop codon, wherein T can also be U;

[0132] (VII) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in FIG. 2F (SEQ ID NO: ______), from nucleotide residue number 281 through nucleotide residue number 850, followed by a stop codon, wherein T can also be U;

[0133] (VIII) a polynucleotide that encodes an 121P1F 1P-related protein that is at least 90% homologous to an entire amino acid sequence shown in FIGS. 2A-F (SEQ ID NO: ______);

[0134] (IX) a polynucleotide that encodes an 121P1F1-related protein that is at least 90% identical to an entire amino acid sequence shown in FIGS. 2A-F (SEQ ID NO: ______);

[0135] (X) a polynucleotide that encodes at least one peptide set forth in Tables V-XVIII, XXVI, and XXVII;

[0136] (XI) a polynucleotide that encodes a peptide region of at least 5 amino acids of a peptide of FIG. 3A in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Hydrophilicity profile of FIG. 5A, or of FIG. 3B in any whole number increment up to 126 that includes an amino acid position having a value greater than 0.5 in the Hydrophilicity profile of FIG. 5B;

[0137] (XII) a polynucleotide that encodes a peptide region of at least 5 amino acids of a peptide of FIG. 3A in any whole number increment up to 205 that includes an amino acid position having a value less than 0.5 in the Hydropathicity profile of FIG. 6A, or of FIG. 3B in any whole number increment up to 126, that includes an amino acid position having a value less than 0.5 in the Hydropathicity profile of FIG. 6B;

[0138] (XIII) a polynucleotide that encodes a peptide region of at least 5 amino acids of a peptide of FIG. 3A in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Percent Accessible Residues profile of FIG. 7A, or of FIG. 3B in any whole number increment up to 126, that includes an amino acid position having a value greater than 0.5 in the Percent Accessible Residues profile of FIG. 7B;

[0139] (XIV) a polynucleotide that encodes a peptide region of at least 5 amino acids of a peptide of FIG. 3A in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Average Flexibility profile on FIG. 8A, or of FIG. 3B in any whole number increment up to 126, that includes an amino acid position having a value greater than 0.5 in the Average Flexibility profile on FIG. 8B;

[0140] (XV) a polynucleotide that encodes a peptide region of at least 5 amino acids of a peptide of FIG. 3A in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Beta-turn profile of FIG. 9A, or of FIG. 3B in any whole number increment up to 126, that includes an amino acid position having a value greater than 0.5 in the Beta-turn profile of FIG. 9B;

[0141] (XVI) a polynucleotide that encodes a 121P1F1-related protein whose sequence is encoded by the cDNAs contained in the plasmid deposited with American Type Culture Collection as Accession No. PTA-3139 on Mar. 1, 2001;

[0142] (XVII) a polynucleotide that is fully complementary to a polynucleotide of any one of (I)-(XVI);

[0143] (XVIII) a polynucleotide that selectively hybridizes under stringent conditions to a polynucleotide of (I)-(XVII);

[0144] (XIX) a peptide that is encoded by any of (I)-(XVIII); and,

[0145] (XX) a polynucleotide of any of (I)-(XVIII)or peptide of (XIX) together with a pharmaceutical excipient and/or in a human unit dose form.

[0146] As used herein, a range is understood to specifically disclose all whole unit positions thereof.

[0147] Typical embodiments of the invention disclosed herein include 121P1F1 polynucleotides that encode specific portions of 121P1F1 mRNA sequences (and those which are complementary to such sequences) such as those that encode the proteins and/or fragments thereof, for example:

[0148] (a) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, or 205 contiguous amino acids of 121P1F1;

[0149] (b) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35,40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, or 126 contiguous amino acids of variant 1A;

[0150] (c) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, or 119 contiguous amino acids of variant IB;

[0151] (d) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, or 122 contiguous amino acids of variant 2; or,

[0152] (e) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, or 190 contiguous amino acids of variant 3; or,

[0153] (f) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, or 190 contiguous amino acids of variant 4.

[0154] For example, representative embodiments of the invention disclosed herein include: polynucleotides and their encoded peptides themselves encoding about amino acid 1 to about amino acid 10 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 10 to about amino acid 20 of the 121P1F 1 protein or variants shown in FIG. 2 or FIG. 3, polynucleolides encoding about amino acid 20 to about amino acid 30 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 30 to about amino acid 40 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 40 to about amino acid 50 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 50 to about amino acid 60 of the 121P1F 1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 60 to about amino acid 70 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 70 to about amino acid 80 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 80 to about amino acid 90 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, polynucleotides encoding about amino acid 90 to about amino acid 100 of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3, in increments of about 10 amino acids, ending at the carboxyl terminal amino acid set forth in FIG. 2 or FIG. 3. Accordingly polynucleotides encoding portions of the amino acid sequence (of about 10 amino acids), of amino acids 100 through the carboxyl terminal amino acid of the 121P1F1 protein are embodiments of the invention. Wherein it is understood that each particular amino acid position discloses that position plus or minus five amino acid residues.

[0155] Polynucleotides encoding relatively long portions of a 121P1F1 protein are also within the scope of the invention. For example, polynucleotides encoding from about amino acid 1 (or 20 or 30 or 40 etc.) to about amino acid 20, (or 30, or 40 or 50 etc.) of the 121P1F1 protein or variants shown in FIG. 2 or FIG. 3 can be generated by a variety of techniques well known in the art. These polynucleotide fragments can include any portion of the 121P1F1 sequence or variants as shown in FIG. 2.

[0156] Additional illustrative embodiments of the invention disclosed herein include 121P1F1 polynucleotide fragments encoding one or more of the biological motifs contained within a 121P1F1 protein sequence or variant sequence, including one or more of the motif-bearing subsequences of a 121P1F1 protein or variant set forth in Tables V-XVIII, XXVI, and XXVII. In another embodiment, typical polynucleotide fragments of the invention encode one or more of the regions of 121P1F1 protein or variant that exhibit homology to a known molecule. In another embodiment of the invention, typical polynucleotide fragments can encode one or more of the 121P1F1 protein or variant N-glycosylation sites, cAMP and cGMP-dependent protein kinase phosphorylation sites, casein kinase II phosphorylation sites or N-myristoylation site and amidation sites.

[0157] II.A.) Uses of 121P1F1 Polynucleotides

[0158] II.A.1.) Monitoring of Genetic Abnormalities

[0159] The polynucleotides of the preceding paragraphs have a number of different specific uses. The human 121P1F1 gene maps to the chromosomal location set forth in Example 3. For example, because the 121P1F1 gene maps to this chromosome, polynucleotides that encode different regions of the 121P1F1 proteins are used to characterize cytogenetic abnormalities of this chromosomal locale, such as abnormalities that are identified as being associated with various cancers. In certain genes, a variety of chromosomal abnormalities including rearrangements have been identified as frequent cytogenetic abnormalities in a number of different cancers (see e.g. Krajinovic et al., Mutat. Res. 382(3-4): 81-83 (1998); Johansson et al., Blood 86(10): 3905-3914 (1995) and Finger et al., P.N.A.S. 85(23): 9158-9162 (1988)). Thus, polynucleotides encoding specific regions of the 121P1F1 proteins provide new tools that can be used to delineate, with greater precision than previously possible, cytogenetic abnormalities in the chromosomal region that encodes 121P1F1 that may contribute to the malignant phenotype. In this context, these polynucleotides satisfy a need in the art for expanding the sensitivity of chromosomal screening in order to identify more subtle and less common chromosomal abnormalities (see e.g. Evans et al., Am. J. Obstet. Gynecol 171(4): 1055-1057 (1994)).

[0160] Furthermore, as 121P1F1 was shown to be highly expressed in bladder and other cancers, 121P1F1 polynucleotides are used in methods assessing the status of 121P1F1 gene products in normal versus cancerous tissues. Typically, polynucleotides that encode specific regions of the 121P1F1 proteins are used to assess the presence of perturbations (such as deletions, insertions, point mutations, or alterations resulting in a loss of an antigen etc.) in specific regions of the 121P1F1 gene, such as regions containing one or more motifs. Exemplary assays include both RT-PCR assays as well as single-strand conformation polymorphism (SSCP) analysis (see, e.g., Marrogi et al., J. Cutan. Pathol. 26(8): 369-378 (1999), both of which utilize polynucleotides encoding specific regions of a protein to examine these regions within the protein.

[0161] II.A.2.) Antisense Embodiments

[0162] Other specifically contemplated nucleic acid related embodiments of the invention disclosed herein are genomic DNA, cDNAs, ribozymes, and antisense molecules, as well as nucleic acid molecules based on an alternative backbone, or including alternative bases, whether derived from natural sources or synthesized, and include molecules capable of inhibiting the RNA or protein expression of 121P1F1. For example, antisense molecules can be RNAs or other molecules, including peptide nucleic acids (PNAs) or non-nucleic acid molecules such as phosphorothioate derivatives, that specifically bind DNA or RNA in a base pair-dependent manner. A skilled artisan can readily obtain these classes of nucleic acid molecules using the 121P1F1 polynucleotides and polynucleotide sequences disclosed herein.

[0163] Antisense technology entails the administration of exogenous oligonucleotides that bind to a target polynucleotide located within the cells. The term “antisense” refers to the fact that such oligonucleotides are complementary to their intracellular targets, e.g., 121P1F1. See for example, Jack Cohen, Oligodeoxynucleotides, Antisense Inhibitors of Gene Expression, CRC Press, 1989; and Synthesis 1:1-5 (1988). The 121P1F1 antisense oligonucleotides of the present invention include derivatives such as S-oligonucleotides (phosphorothioate derivatives or S-oligos, see, Jack Cohen, supra), which exhibit enhanced cancer cell growth inhibitory action. S-oligos (nucleoside phosphorothioates) are isoelectronic analogs of an oligonucleotide (O-oligo) in which a nonbridging oxygen atom of the phosphate group is replaced by a sulfur atom. The S-oligos of the present invention can be prepared by treatment of the corresponding O-oligos with 3H-1,2-benzodithiol-3-one-1,1-dioxide, which is a sulfur transfer reagent. See, e.g., Iyer, R. P. et al., J. Org. Chem. 55:46934698 (1990); and Iyer, R. P. et al., J. Am. Chem. Soc. 112:1253-1254 (1990). Additional 121P1F1 antisense oligonucleotides of the present invention include morpholinc antisense oligonucleotides known in the art (see, e.g., Partridge et al., 1996, Antisense & Nucleic Acid Drug Development 6: 169-175).

[0164] The 121P1F1 antisense oligonucleotides of the present invention typically can be RNA or DNA that is complementary to and stably hybridizes with the first 100 5′ codons or last 100 3′ codons of a 121P1F1 genoric sequence or the corresponding mRNA. Absolute complementarity is not required, although high degrees of complementarity are preferred. Use of an oligonucleotide complementary to this region allows for the selective hybridization to 121P1F1 mRNA and not to mRNA specifying other regulatory subunits of protein kinase. In one embodiment, 121P1F1 antisense oligonucleotides of the present invention are 15 to 30-mer fragments of the antisense DNA molecule that have a sequence that hybridizes to 121P1F1 mRNA. Optionally, 121P1F1 antisense oligonucleotide is a 30-mer oligonucleotide that is complementary to a region in the first 10 5′ codons or last 10 3′ codons of 121P1F1. Alternatively, the antisense molecules are modified to employ ribozymes in the inhibition of 121P1F1 expression, see, e.g., L. A. Couture & D. T. Stinchcomb; Trends Genet 12: 510-515 (1996).

[0165] II.A.3.) Primers and Primer Pairs

[0166] Further specific embodiments of this nucleotides of the invention include primers and primer pairs, which allow the specific amplification of polynucleotides of the invention or of any specific parts thereof, and probes that selectively or specifically hybridize to nucleic acid molecules of the invention or to any part thereof. Probes can be labeled with a detectable marker, such as, for example, a radioisotope, fluorescent compound, bioluminescent compound, a chemiluminescent compound, metal chelator or enzyme. Such probes and primers are used to detect the presence of a 121P1F1 polynucleotide in a sample and as a means for detecting a cell expressing a 121P1F1 protein.

[0167] Examples of such probes include polypeptides comprising all or part of the human 121P1F1 cDNA sequence shown in FIG. 2. Examples of primer pairs capable of specifically amplifying 121P1F1 mRNAs are also described in the Examples. As will be understood by the skilled artisan, a great many different primers and probes can be prepared based on the sequences provided herein and used effectively to amplify and/or detect a 121P1F1 mRNA.

[0168] The 121P1F1 polynucleotides of the invention are useful for a variety of purposes, including but not limited to their use as probes and primers for the amplification and/or detection of the 121P1F1 gene(s), mRNA(s), or fragments thereof; as reagents for the diagnosis and/or prognosis of prostate cancer and other cancers; as coding sequences capable of directing the expression of 121P1F1 polypeptides; as tools for modulating or inhibiting the expression of the 121P1F1 gene(s) and/or translation of the 121P1F1 transcript(s); and as therapeutic agents.

[0169] The present invention includes the use of any probe as described herein to identify and isolate a 121P1F1 or 121P1F1 related nucleic acid sequence from a naturally occurring source, such as humans or other mammals, as well as the isolated nucleic acid sequence per se, which would comprise all or most of the sequences found in the probe used.

[0170] II.A.4.) Isolation of 121P1F1-Encoding Nucleic Acid Molecules

[0171] The 121P1F1 cDNA sequences described herein enable the isolation of other polynucleotides encoding 121P1F1 gene product(s), as well as the isolation of polynucleotides encoding 121P1F1 gene product homologs, alternatively spliced isoforms, allelic variants, and mutant forms of a 121P1F1 gene product as well as polynucleotides that encode analogs of 121P1F1-related proteins. Various molecular cloning methods that can be employed to isolate full length cDNAs encoding an 121P1F1 gene are well known (see, for example, Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, 2d edition, Cold Spring Harbor Press, New York, 1989; Current Protocols in Molecular Biology. Ausubel et al., Eds., Wiley and Sons, 1995). For example, lambda phage cloning methodologies can be conveniently employed, using commercially available cloning systems (e.g., Lambda ZAP Express, Stratagene). Phage clones containing 121P1F1 gene cDNAs can be identified by probing with a labeled 121P1F1cDNA or a fragment thereof. For example, in one embodiment, a 121P1F1 cDNA (e.g., FIG. 2) or a portion thereof can be synthesized and used as a probe to retrieve overlapping and full-length cDNAs corresponding to a 121P1F1 gene. A 121P1F1 gene itself can be isolated by screening genomic DNA libraries, bacterial artificial chromosome libraries (BACs), yeast artificial chromosome libraries (YACs), and the like, with 121P1F1 DNA probes or primers.

[0172] II.A.5.) Recombinant Nucleic Acid Molecules and Host-Vector Systems

[0173] The invention also provides recombinant DNA or RNA molecules containing an 121P1F1 polynucleolide, a fragment, analog or homologue thereof, including but not limited to phages, plasmids, phagemnids, cosmids, YACs, BACs, as well as various viral and non-viral vectors well known in the art, and cells transformed or transfected with such recombinant DNA or RNA molecules. Methods for generating such molecules are well known (see, for example, Sambrook et al., 1989, supra).

[0174] The invention further provides a host-vector system comprising a recombinant DNA molecule containing a 121P1F1 polynucleotide, fragment, analog or homologue thereof within a suitable prokaryotic or eukaryotic host cell. Examples of suitable eukaryotic host cells include a yeast cell, a plant cell, or an animal cell., such as a mammalian cell or an insect cell (e.g., a baculovirus-infectible cell such as an Sf9 or HighFive cell). Examples of suitable mammalian cells include various prostate cancer cell lines such as DU145 and. TsuPr1, other transfectable or transducible prostate cancer cell lines, primary cells (PrEC), as well as a number of mammalian cells routinely used for the expression of recombinant proteins (e.g., COS, CHO, 293, 293T cells). More particularly, a polynucleotide comprising the coding sequence of 121P1F1 or a fragment, analog or homolog thereof can be used to generate 121P1F1 proteins or fragments thereof using any number of host-vector systems routinely used and widely known in the art.

[0175] A wide range of host-vector systems suitable for the expression of 121P1F1 proteins or fragments thereof are available, see for example, Sambrook et al., 1989, supra; Current Protocols in Molecular Biology, 1995, supra). Preferred vectors for mammalian expression include but are not limited to pcDNA 3.1 myc-His-tag (Invitrogen) and the retroviral vector pSRextkneo (Muller et al., 1991, MCB 11:1785). Using these expression vectors, 121P1F1 can be expressed in several prostate cancer and non-prostate cell lines, including for example 293, 293T, rat-1, NIH 3T3 and TsuPrl. The host-vector systems of the invention are useful for the production of a 121P1F1 protein or fragment thereof. Such host-vector systems can be employed to study the functional properties of 121P1F1 and 121P1F1 mutations or analogs.

[0176] Recombinant human 121P1F1 protein or an analog or homolog or fragment thereof can be produced by mammalian cells transfected with a construct encoding a 121P1F1-related nucleotide. For example, 293T cells can be transfected with an expression plasmid encoding 121P1F1 or fragment, analog or homolog thereof, a 121P1F1-related protein is expressed in the 293T cells, and the recombinant 121P1F1 protein is isolated using standard purification methods (e.g., affinity purification using anti-121P1F1 antibodies). In another embodiment, a 121P1F1 coding sequence is subcloned into the retroviral vector pSRαMSVtkneo and used to infect various mammalian cell lines, such as NIH 3T3, TsuPr1, 293 and rat-1 in order to establish 121P1F1 expressing cell lines. Various other expression systems well known in the art can also be employed. Expression constructs encoding a leader peptide joined in frame to a

[0177]121P1F1 coding sequence can be used for the generation of a secreted form of recombinant 121P1F1 protein.

[0178] As discussed herein, redundancy in the genetic code permits variation in 121P1F1 gene sequences. In particular, it is known in the art that specific host species often have specific codon preferences, and thus one can adapt the disclosed sequence as preferred for a desired host. For example, preferred analog codon sequences typically have rare codons (i.e., codons having a usage frequency of less than about 20% in known sequences of the desired host) replaced with higher frequency codons. Codon preferences for a specific species are calculated, for example, by utilizing codon usage tables available on the INTERNET such as at URL www.dna.affrc.go.jp/nakamura/codon.html.

[0179] Additional sequence modifications are known to enhance protein expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon/intron splice site signals, transposon-like repeats, and/or other such well-characterized sequences that are deleterious to gene expression. The GC content of the sequence is adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. Where possible, the sequence is modified to avoid predicted hairpin secondary mRNA structures. Other useful modifications include the addition of a translational initiation consensus sequence at the start of the open reading frame, as described in Kozak, Mol. Cell Biol., 9:5073-5080 (1989). Skilled artisans understand that the general rule that eukaryotic ribosomes initiate translation exclusively at the 5′ proximal AUG codon is abrogated only under rare conditions (see, e.g., Kozak PNAS 92(7): 2662-2666, (1995) and Kozak NAR 15(20): 8125-8148 (1987)).

[0180] III.) 121P1F1-Related Proteins

[0181] Another aspect of the present invention provides 121P1F 1-related proteins. Specific embodiments of 121P1F proteins comprise a polypeptide having all or part of the amino acid sequence of human 121P1F1 as, shown in FIG. 2 or FIG. 3. Alternatively, embodiments of 121P1F1 proteins comprise variant, homolog or analog polypeptides that have alterations in the amino acid sequence of 121P1F1 shown in FIG. 2 or FIG. 3.

[0182] In general, naturally occurring allelic variants of human 121P1F 1 share a high degree of structural identity and homology (e.g., 90% or more homology). Typically, allelic variants of a 121P1F1 protein contain conservative amino acid substitutions within the 121P1F1 sequences described herein or contain a substitution of an amino acid from a corresponding position in a homologue of 121P1F1. One class of 121P1F1 allelic variants are proteins that share a high degree of homology with at least a small region of a particular 121P1F1 amino acid sequence, but further contain a radical departure from the sequence, such as a non-conservative substitution, truncation, insertion or frame shift. In comparisons of protein sequences, the terms, similarity, identity, and homology each have a distinct meaning as appreciated in the field of genetics. Moreover, orthology and paralogy can be important concepts describing the relationship of members of a given protein family in one organism to the members of the same family in other organisms.

[0183] Amino acid abbreviations are provided in Table II. Conservative amino acid substitutions can frequently be made in a protein without altering either the conformation or the function of the protein. Proteins of the invention can comprise 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 conservative substitutions. Such changes include substituting any of isoleucine (I), valine (V), and leucine (L) for any other of these hydrophobic amino acids; aspartic acid (D) for glutamic acid (E) and vice versa; glutamine (O) for asparagine (N) and vice versa; and serine (S) for threonine (T) and vice versa. Other substitutions can also be considered conservative, depending on the environment of the particular amino acid and its role in the three-dimensional structure of the protein. For example, glycine (G) and alanine (A) can frequently be interchangeable, as can alanine (A) and valine (V). Methionine (M), which is relatively hydrophobic, can frequently be interchanged with leucine and isoleucine, and sometimes with valine. Lysine (K) and arginine (R) are frequently interchangeable in locations in which the significant feature of the amino acid residue is its charge and the differing pK's of these two amino acid residues are not significant. Still other changes can be considered “conservative” in particular environments (see, e.g. Table III herein; pages 13-15 “Biochemistry” 2^(nd) ED. Lubert Stryer ed (Stanford University); Henikoff et al., PNAS 1992 Vol 89 10915-10919; Lei et al., J Biol Chem May 19, 1995; 270(20):11882-6).

[0184] Embodiments of the invention disclosed herein include a wide variety of art-accepted variants or analogs of 121P1F1 proteins such as polypeptides having amino acid insertions, deletions and substitutions. 121P1F1 variants can be made using methods known in the art such as site-directed mutagenesis, alanine scanning, and PCR mutagenesis. Site-directed mutagenesis (Carter et al., Nucl. Acids Res., 13:4331 (1986); Zoller et al., Nucl. Acids Res., 10:6487 (1987)), cassette mutagenesis (Wells etal., Gene, 34:315 (1985)), restriction selection mutagenesis (Wells et al., Philos. Trans. R. Soc. London SerA, 317:415 (1986)) or other known techniques can be performed on the cloned DNA to produce the 121P1F1 variant DNA.

[0185] Scanning amino acid analysis can also be employed to identify one or more amino acids along a contiguous sequence that is involved in a specific biological activity such as a protein-protein interaction. Among the preferred scanning amino acids are relatively small, neutral amino acids. Such amino acids include alanine, glycine, serine, and cysteine. Alanine is typically a preferred scanning amino acid among this group because it eliminates the side-chain beyond the beta-carbon and is less likely to alter the main-chain conformation of the variant. Alanine is also typically preferred because it is the most common amino acid. Further, it is frequently found in both buried and exposed positions (Creighton, The Proteins, (W. H. Freeman & Co., N.Y.); Chothia, J. Mol. Biol., 150:1 (1976)). If alanine substitution does not yield adequate amounts of variant, an isosteric amino acid can be used.

[0186] As defined herein, 121P1F1 variants, analogs or homologs, have the distinguishing attribute of having at least one epitope that is “cross reactive” with a 121P1F1 protein having an amino acid sequence of FIG. 3. As used in this sentence, “cross reactive” means that an antibody or T cell that specifically binds to an 121P1F1 variant also specifically binds to a 121P1F1 protein having an amino acid sequence set forth in FIG. 3. A polypeptide ceases to be a variant of a protein shown in FIG. 3, when it no longer contains any epitope capable of being recognized by an antibody or T cell that specifically binds to the starting 121P1F1 protein. Those skilled in the art understand that antibodies that recognize proteins bind to epitopes of varying size, and a grouping of the order of about four or five amino acids, contiguous or not, is regarded as a typical number of amino acids in a minimal epitope. See, e.g., Nair et al., J. Immunol 2000 165(12): 6949-6955; Hebbes et al., Mol Immunol (1989) 26(9):865-73; Schwartz et al., J Immunol (1985) 135(4):259E8-608.

[0187] Other classes of 121P1F1-related protein variants share 70%, 75%, 80%, 85% or 90% or more similarity with an amino acid sequence of FIG. 3, or a fragment thereof. Another specific class of 121P1F1 protein variants or analogs comprise one or more of the 121P1F1 biological motifs described herein or presently known in the art. Thus, encompassed by the present invention are analogs of 121P1F1 fragments (nucleic or amino acid) that have altered functional (e.g. immunogenic) properties relative to the starting fragment. It is to be appreciated that motifs now or which become part of the art are to be applied to the nucleic or amino acid sequences of FIG. 2 or FIG. 3.

[0188] As discussed herein, embodiments of the claimed invention include polypeptides containing less than the full, amino acid sequence of a 121P1F1 protein shown in FIG. 2 or FIG. 3. For example, representative embodiments of the invention comprise peptides/proteins having any 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more contiguous amino acids of a 121P1F1 protein shown in FIG. 2 or FIG. 3.

[0189] Moreover, representative embodiments of the invention disclosed herein include polypeptides consisting of about amino acid 1 to about amino acid 10 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 10 to about amino acid 20 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 20 to about amino acid 30 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 30 to about amino acid 40 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 40 to about amino acid 50 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 50 to about amino acid 60 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 60 to about amino acid 70 of a 121P1F 1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 70 to about amino acid 80 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 80 to about amino acid 90 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, polypeptides consisting of about amino acid 90 to about amino acid 100 of a 121P1F1 protein shown in FIG. 2 or FIG. 3, etc. throughout the entirety of a 121P1F1 amino acid sequence. Moreover, polypeptides consisting of about amino acid 1 (or 20 or 30 or 40 etc.) to about amino acid 20, (or 130, or 140 or 150 etc.) of a 121P1F1 protein shown in FIG. 2 or FIG. 3 are embodiments of the invention. It is to be appreciated that the starting and stopping positions in this paragraph refer to the specified position as well as that position plus or minus 5 residues.

[0190] 121P1F1-related proteins are generated using standard peptide synthesis technology or using chemical cleavage methods well known in the art. Alternatively, recombinant methods can be used to generate nucleic acid molecules that encode a 121P1F1-related protein. In one embodiment, nucleic acid molecules provide a means to generate defined fragments of a 121P1F1 protein (or variants, homologs or analogs thereof).

[0191] III.A.) Motif-bearing Protein Embodiments

[0192] Additional illustrative embodiments of the invention disclosed herein include 121P1F1 polypeptides comprising the amino acid residues of one or more of the biological motifs contained within a 121P1F1 polypeptide sequence set forth in FIG. 2 or FIG. 3. Various motifs are known in the art, and a protein can be evaluated for the presence of such motifs by a number of publicly available Internet sites (see, e.g., URL addresses: pfam.wustl.edu/; http://searchlauncher.bcm.tmc.edu/seq-search/struc-predict.htmi; psort.ims.u-tokyo.ac.jp/; www.cbs.dtu.dk/; www.ebi.ac.uk/interpro/scan.html; www.expasy.ch/tools/scnpsit1.html; Epimatrix™ and Epimer™, Brown University, www.browi.edu/ResearchiTB-HIV_Lab/epimattix/epimatrix.html; and BIMAS, bimas.dcrt.nih.gov/.).

[0193] Motif bearing subsequences of all 121P1F1 variant proteins are set forth and identified in Table XIX.

[0194] Table XX sets forth several frequently occurring motifs based on pfam searches (see URL address pfam.wustl.edu/). The columns of Table XX list (1) motif name abbreviation, (2) percent identity found amongst the different member of the motif family, (3) motif name or description and (4) most common function; location information is included if the motif is relevant for location.

[0195] Polypeptides comprising one or more of the 121P1F1 motifs discussed above are useful in elucidating the specific characteristics of a malignant phenotype in view of the observation that the 121P1F1 motifs discussed above are associated with growth dysregulation and because 121P1F1 is overexpressed in certain cancers (See, e.g., Table I). Casein kinase II, cAMP and camp-dependent protein kinase, and Protein Kinase C, for example, are enzymes known to be associated with the development of the malignant phenotype (see e.g. Chen et al., Lab Invest., 78(2): 165-174 (1998); Gaiddon et al., Endocrinology 136(10): 43314338 (1995); Hall et al., Nucleic Acids Research 24(6): 1119-1126 (1996); Peterziel et al., Oncogene 18(46): 6322-6329 (1999) and O'Brian, Oncol. Rep. 5(2): 305-309 (1998)). Moreover, both glycosylation and myristoylation are protein modifications also associated with cancer and cancer progression (see e.g. Dennis et al., Biochem. Biophys. Acta 1473(1):21-34 (1999); Raju et al., Exp. Cell Res. 235(1): 145-154 (1997)). Amidation is another protein modification also associated with cancer and cancer progression (see e.g. Treston et al., J. Natl. Cancer Inst. Monogr. (13): 169-175 (1992)).

[0196] In another embodiment, proteins of the invention comprise one or more of the immunoreactive epitopes identified in accordance with art-accepted methods, such as the peptides set forth in Tables V-XVIII, XXVI, and XXVII. CTL epitopes can be determined using specific algorithms to identify peptides within an 121P1F1 protein that are capable of optimally binding to specified HLA alleles (e.g., Table IV; Epimatrix™ and Epimer™, Brown University, URL www.brown.edu/ResearchITB-HIV_Lab/epimatrix/epimatrix.html; and BIAS, URL bimas.dcrt.nih.gov/.) Moreover, processes for identifying peptides that have sufficient binding affinity for HLA molecules and which are correlated with being immunogenic epitopes, are well known in the art, and are carried out without undue experimentation. In addition, processes for identifying peptides that are immunogenic epitopes, are well known in the art, and are carried out without undue experimentation either in vitro or in vivo.

[0197] Also known in the art are principles for creating analogs of such epitopes in order to modulate immunogenicity. For example, one begins with an epitope that bears a CTL or HTL motif (see, e.g., the HLA Class I and HLA Class II motifs/supermotifs of Table IV). The epitope is analoged by substituting out an amino acid at one of the specified positions, and replacing it with another amino acid specified for that position. For example, one can substitute out a deleterious residue in favor of any other residue, such as a preferred residue as defined in Table IV; substitute a less-preferred residue with a preferred residue as defined in Table IV; or substitute an originally-occurring preferred residue with another preferred residue as defined in Table IV. Substitutions can occur at primary anchor positions or at other positions in a peptide; see, e.g., Table IV.

[0198] A variety of references reflect the art regarding the identification and generation of epitopes in a protein of interest as well as analogs thereof. See, for example, WO 9733602 to Chesnut et al.; Sette, Immunogenetics 1999 50(3-4): 201-212; Sette et al., J. Immunol. 2001 166(2): 1389-1397; Sidney et al., Hum. Immunol. 1997 58(1): 12-20; Kondo et al., Immunogenetics 1997 45(4): 249-258; Sidney et al., J. Immunol. 1996 157(8): 3480-90; and Falk et al., Nature 351: 290-6 (1991); Hunt et al., Science 255:1261-3 (1992); Parker et al., J. Immunol. 149:3580-7 (1992); Parker et al., J. Immunol. 152:163-75 (1994)); Kast et al., 1994 152(8): 3904-12; Borras-Cuesta et al., Hum. Immunol. 2000 61(3): 266-278; Alexander et al., J. Immunol. 2000 164(3); 164(3): 1625-1633; Alexander et al., PMID: 7895164, UI: 95202582; O'Sullivan et al., J. Immunol. 1991 147(8): 2663-2669; Alexander et al., Immunity 1994 1(9): 751-761 and Alexander et al., Immunol. Res. 1998 18(2): 79-92.

[0199] Related embodiments of the inventions include polypeptides comprising combinations of the different motifs set forth in Table XIX, and/or, one or more of the predicted CTL epitopes of Table V through Table XVIII, and/or, one or more of the T cell binding motifs known in the art. Preferred embodiments contain no insertions, deletions or substitutions either within the motifs or the intervening sequences of the polypeptides. In addition, embodiments which include a number of either N-terminal and/or C-terminal amino acid residues on either side of these motifs may be desirable (to, for example, include a greater portion of the polypeptide architecture in which the motif is located). Typically the number of N-terminal and/or C-terminal amino acid residues on either side of a motif is between about 1 to about 100 amino acid residues, preferably 5 to about 50 amino acid residues.

[0200] 121P1F1-related proteins are embodied in many forms, preferably in isolated form A purified 121P1F1 protein molecule will be substantially free of other proteins or molecules that impair the binding of 121P1F1 to antibody, T cell or other ligand. The nature and degree of isolation and purification will depend on the intended use. Embodiments of a 121P1F1-related proteins include purified 121P1F1-related proteins and functional, soluble 121P1F1-related proteins. In one embodiment, a functional, soluble 121P1F1 protein or fragment thereof retains the ability to be bound by antibody, T cell or other ligand.

[0201] The invention also provides 121P1F1 proteins comprising biologically active fragments of a 121P1F1 amino acid sequence shown in FIG. 2 or FIG. 3. Such proteins exhibit properties of the starting 121P1F1 protein, such as the ability to elicit the generation of antibodies that specifically bind an epitope associated with the starting 121P1F1 protein; to be bound by such antibodies; to elicit the activation of HTL or CTL; and/or, to be recognized by HTL or CTL that also specifically bind to the starting protein.

[0202] 121P1F 1-related polypeptides that contain particularly interesting structures can be predicted and/or identified using various analytical techniques well known in the art, including, for example, the methods of Chou-Fasman, Gamnier-Robson, Kyte-Doolittle, Eisenberg, Karplus-Schultz or Jameson-Wolf analysis, or on the basis of inmmunogenicity. Fragments that contain such structures are particularly useful in generating subunit-specific anti-121P1F1 antibodies, or T cells or in identifying cellular factors that bind to 121P1F1. For example, hydrophilicity profiles can be generated, and immunogenic peptide fragments identified, using the method of Hopp, T. P. and Woods, K. R., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:3824-3828. Hydropathicity profiles can be generated, and immunogenic peptide fragments identified, using the method of Kyte, J. and Doolittle, R. F., 1982, J. Mol. Biol. 157:105-132. Percent (%) Accessible Residues profiles can be generated, and immunogenic peptide fragments identified, using the method of Janin J., 1979, Nature 277:491-492. Average Flexibility profiles can be generated, and immunogenic peptide fragments identified, using the method of Bhaskaran R., Ponnuswamy P. K., 1988, Int. J. Pept. Protein Res. 32:242-255. Beta-turn profiles can be generated, and immunogenic peptide fragments identified, using the method of Deleage, G., Roux B., 1987, Protein Engineering 1:289-294.

[0203] CTL epitopes can be determined using specific algorithms to identify peptides within an 121P1F1 protein that are capable of optimally binding to specified HLA alleles (e.g., by using the SYFPEITHI site at World Wide Web URL syfpeithi.bmi-heidelberg.com/; the listings in Table IV(A)-(E); Epimatrix™ and Epimer™, Brown University, URL (www.brown.edu/ResearchITB-HIV_Lab/epimatrix/epimatrix.html); and BIMAS, URL bimas.dcrt.nih.gov/). Illustrating this, peptide epitopes from 121P1F1 that are presented in the context of human MHC class I molecules HLA-A1, A2, A3, A11, A24, B7 and B35 were predicted (Tables V-XVIII, XXVI, and XXVII). Specifically, the complete amino acid sequence of the 121P1F1 protein and relevant portions of other variants, i.e., for HLA Class I predictions 9 flanking redisues on either side of a point mutation, and for HLA Class II predictions 14 flanking residues on either side of a point mutation, were entered into the HLA Peptide Motif Search algorithm found in the Bioinformatics and Molecular Analysis Section (BIMAS) web site listed above; for HLA Class II the site SYFPEITHI at URL syfpeithi.bmi-heidelberg.com/ was used.

[0204] The HLA peptide motif search algorithm was developed by Dr. Ken Parker based on binding of specific peptide sequences in the groove of HLA Class I molecules, in particular HLA-A2 (see, e.g., Falk et al., Nature 351: 290-6 (1991); Hunt et al., Science 255:1261-3 (1992); Parker et al., J. Immunol. 149:3580-7 (1992); Parker et al., J. Immunol. 152:163-75 (1994)). This algorithm allows location and ranking of 8-mer, 9-mer, and 10-mer peptides from a complete protein sequence for predicted binding to HLA-A2 as well as numerous other HLA Class I molecules. Many HLA class I binding peptides are 8-, 9-, 10 or 11-mers. For example, for class I HLA-A2, the epitopes preferably contain a leucine (L) or methionine (M) at position 2 and a valine (V) or leucine (L) at the C-terminus (see, e.g., Parker et al., J. Immunol. 149:3580-7 (1992)). Selected results of 121P1F1 predicted binding peptides are shown in Tables V-XVIII, XXVI, and XXVII herein. In Tables V-XVIII, the top 50 ranking candidates, 9-mers and 10-mers, for each family member are shown along with their location, the amino acid sequence of each specific peptide, and an estimated binding score. The binding score corresponds to the estimated half time of dissociation of complexes containing the peptide at 37° C. at pH 6.5. Peptides with the highest binding score are predicted to be the most tightly bound to HLA Class I on the cell surface for the greatest period of time and thus represent the best immunogenic targets for T-cell recognition.

[0205] Actual binding of peptides to an HLA allele can be evaluated by stabilization of HLA expression on the antigen-processing defective cell line T2 (see, e.g., Xue et al., Prostate 30:73-8 (1997) and Peshwa et al., Prostate 36:129-38 (1998)). Immunogenicity of specific peptides can be evaluated in vitro by stimulation of CD8+ cytotoxic T lymphocytes (CTL) in the presence of antigen presenting cells such as dendritic cells.

[0206] It is to be appreciated that every epitope predicted by the BIMAS site, Epimer™ and Epimatrix™ sites, or specified by the HLA class I or class II motifs available in the art or which become part of the art such as set forth in Table IV (or determined using World Wide Web site URL syfpeithi.bmi-heidelberg.com/, or BIMAS, bimas.dcrt.nih.gov/) are to be “applied” to a 121P1F1 protein in accordance with the invention. As used in this context “applied” means that a 121P1F1 protein is evaluated, e.g., visually or by computer-based patterns finding methods, as appreciated by those of skill in the relevant art. Every subsequence of a 121P1F1 protein of 8, 9, 10, or 11 amino acid residues that bears an HLA Class I motif, or a subsequence of 9 or more amino acid residues that bear an HLA Class II motif are within the scope of the invention.

[0207] III.B.) Expression of 121P1F1-Related Proteins

[0208] In an embodiment described in the examples that follow, 121P1F1 can be conveniently expressed in cells (such as 293T cells) transfected with a commercially available expression vector such as a CMV-driven expression vector encoding 121P1F1 with a C-terminal 6×His and MYC tag (pcDNA3.1/mycHIS, Invitrogen or Tag5, GenHunter Corporation, Nashville Tenn.). The Tag5 vector provides an IgGK secretion signal that c(an be used to facilitate the production of a secreted 121P1F1 protein in transfected cells. The secreted HIS-tagged 121P1F1 in the culture media can be purified, e.g., using a nickel column using standard techniques.

[0209] III:.C.) Modifications of 121P1F1-Related Proteins

[0210] Modifications of 121P1F1-related proteins such as covalent modifications are included within the scope of this invention. One type of covalent modification includes reacting targeted amino acid residues of a 121P1F1 polypeptide with an organic derivatizing agent that is capable of reacting with selected side chains or the N- or C-terminal residues of a 121P1F1 protein. Another type of covalent modification of a 121P1F1 polypeptide included within the scope of this invention comprises altering the native glycosylation pattern of a protein of the invention. Another type of covalent modification of 121P1F1 comprises linking a 121P1F1 polypeptide to one of a variety of nonproteinaceous polymers, e.g., polyethylene glycol (PEG), polypropylene glycol, or polyoxyalkylenes, in the manner set forth in U.S. Pat. Nos. 4,640,835; 4,496,689; 4,301,144; 4,670,417; 4,791,192 or 4,179,337.

[0211] The 121P1F1-related proteins of the present invention can also be modified to form a chimeric molecule comprising 121P1F1 fused to another, heterologous polypeptide or amino acid sequence. Such a chimeric molecule can be synthesized chemically or recombinantly. A chimeric molecule can have a protein of the invention fused to another tumor-associated antigen or fragment thereof. Alternatively, a protein in accordance with the invention can comprise a fusion of fragments of a 121P1F1 sequence (amino or nucleic acid) such that a molecule is created that is not, through its length, directly homologous to the amino or nucleic acid sequences shown in FIG. 2 or FIG. 3. Such a chimeric molecule can comprise multiples of the same subsequence of 121P1F1. A chimeric molecule can comprise a fusion of a 121P1F1-related protein with a polyhistidine epitope tag, which provides an epitope to which immobilized nickel can selectively bind, with cytokines or with growth factors. The epitope tag is generally placed at the amino- or carboxyl-terminus of a 121P1F1 protein. In an alternative embodiment, the chimeric molecule can comprise a fusion of a 121P1F1-related protein with an immunoglobulin or a particular region of an immunoglobulin. For a bivalent form of the chimeric molecule (also referred to as an “inmmunoadhesin”), such a fusion could be to the Fc region of an IgG molecule. The Ig fusions preferably include the substitution of a soluble (transmembrane domain deleted or inactivated) form of a 121P1F1 polypeptide in place of at least one variable region within an Ig molecule. In a preferred embodiment, the immunoglobulin fusion includes the hinge, CH2 and CH3, or the hinge, CH1, CH2 and CH3 regions of an IgGI molecule. For the production of immunoglobulin fusions see, e.g., U.S. Pat. No. 5,428,130 issued Jun. 27, 1995.

[0212] III:.D.) Uses of 121P1F1-Related Proteins

[0213] The proteins of the invention have a number of different specific uses. As 121P1F1 is highly expressed in prostate and other cancers, 121P1F1-related proteins are used in methods that assess the status of 121P1F1 gene products in normal versus cancerous tissues, thereby elucidating the malignant phenotype. Typically, polypeptides from specific regions of a 121P1F1 protein are used to assess the presence of perturbations (such as deletions, insertions, point mutations etc.) in those regions (such as regions containing one or more motifs). Exemplary assays utilize antibodies or T cells targeting 121P1F1-related proteins comprising the amino acid residues of one or more of the biological motifs contained within a 121P1F1 polypeptide sequence in order to evaluate the characteristics of this region in normal versus cancerous tissues or to elicit an immune response to the epitope. Alternatively, 121P1F1-related proteins that contain the amino acid residues of one or more of the biological motifs in a 121P1F1 protein are used to screen for factors that interact with that region of 121P1F1.

[0214] 121P1F1 protein fragments/subsequences are particularly useful in generating and characterizing domain-specific antibodies (e.g., antibodies recognizing an extracellular or intracellular epitope of an 121P1F1 protein), for identifying agents or cellular factors that bind to 121P1F1 or a particular structural domain thereof, and in various therapeutic and diagnostic contexts, including but not limited to diagnostic assays, cancer vaccines and methods of preparing such vaccines.

[0215] Proteins encoded by the 121P1F1 genes, or by analogs, homologs or fragments thereof, have a variety of uses, including but not limited to generating antibodies and in methods for identifying ligands and other agents and cellular constituents that bind to an 121P1F1 gene product. Antibodies raised against an 121P1F1 protein or fragment thereof are useful in diagnostic and prognostic assays, and imaging methodologies in the management of human cancers characterized by expression of 121P1F1 protein, such as those listed in Table I. Such antibodies can be expressed intracellularly and used in methods of treating patients with such cancers. 121P1F1-related nucleic acids or proteins are also used in generating HTL or CTL responses.

[0216] Various immunological assays useful for the detection of 121P1F1 proteins are used, including but not limited to various types of radioimmunoassays, enzyme-linked immunosorbent assays (ELISA), enzyme-linked inmmunofluorescent assays (ELIFA), immunocytochemical methods, and the like. Antibodies can be labeled and used as immunological imaging reagents capable of detecting 121P1F1-expressing cells (e.g., in radioscintigiaphic imaging methods). 121P1F1 proteins are also particularly useful in generating cancer vaccines, as further described herein.

[0217] IV.) 121P1F1 Antibodies

[0218] Another aspect of the invention provides antibodies that bind to 121P1F1-related proteins. Preferred antibodies specifically bind to a 121P1F1-related protein and do not bind (or bind weakly) to peptides or proteins that are not 121P1F1-related proteins. For example, antibodies that bind 121P1F1 can bind 121P1F1-related proteins such as the homologs or analogs thereof.

[0219] 121P1F1 antibodies of the invention are particularly useful in cancer (see, e.g., Table I) diagnostic and prognostic assays, and imaging methodologies. Similarly, such antibodies are useful in the treatment, diagnosis, and/or prognosis of other cancers, to the extent 121P1F1 is also expressed or overexpressed in these other cancers. Moreover, intracellularly expressed antibodies (e.g., single chain antibodies) are therapeutically useful in treating cancers in which the expression of 121P1F1 is involved, such as advanced or metastatic prostate cancers.

[0220] The invention also provides various immunological assays useful for the detection and quantification of 121P1F1 and mutant 121P1F1-related proteins. Such assays can comprise one or more 121P1F1 antibodies capable of recognizing and binding a 121P1F1-related protein, as appropriate. These assays are performed within various immunological assay formats well known in the art, including but not limited to various types of radioimmunoassays, enzyme-linked immunosorbent assays (ELISA), enzyme-linked immunofluorescent assays (ELIFA), and the like.

[0221] Immunological non-antibody assays of the invention also comprise T cell immunogenicity assays (inhibitory or stimulatory) as well as major histocompatibility complex (MHC) binding assays.

[0222] In addition, immunological imaging methods capable of detecting prostate cancer and other cancers expressing 121P1F1 are also provided by the invention, including but not limited to radioscintigraphic imaging methods using labeled 121P1F1 antibodies. Such assays are clinically useful in the detection, monitoring, and prognosis of 121P1F1 expressing cancers such as prostate cancer.

[0223] 121P1F1 antibodies are also used in methods for purifying a 121P1F1-related protein and for isolating 121P1F 1 homologues and related molecules. For example, a method of purifying a 121P1F 1-related protein comprises incubating an 121P1F1 antibody, which has been coupled to a solid matrix, with a lysate or other solution containing a 121P1F1-related protein under conditions that permit the 121P1F1 antibody to bind to the 121P1F1-related protein; washing the solid matrix to eliminate impurities; and eluting the 121P1F1-related protein from the coupled antibody. Other uses of 121P1F1 antibodies in accordance with the invention include generating anti-idiotypic antibodies that mimic a 121P1F1 protein.

[0224] Various methods for the preparation of antibodies are well known in the art. For example, antibodies can be prepared by immunizing a suitable mammalian host using a 121P1F1-related protein, peptide, or fragment, in isolated or immunoconjugated form (Antibodies: A Laboratory Manual, CSH Press, Eds., Harlow, and Lane (1988); Harlow, Antibodies, Cold Spring Harbor Press, NY (1989)). In addition, fusion proteins of 121P1F1 can also be used, such as a 121P1F1 GST-fusion protein. In a particular embodiment, a GST fusion protein comprising all or most of the amino acid sequence of FIG. 2 or FIG. 3 is produced, then used as an immunogen to generate appropriate antibodies. In another embodiment, a 121P1F1-related protein is synthesized and used as an immunogen.

[0225] In addition, naked DNA immunization techniques known in the art are used (with or without purified 121P1F1-related protein or 121P1F1 expressing cells) to generate an immune response to the encoded immunogen (for review, see Donnelly et al., 1997, Ann. Rev. Immunol. 15: 617-648).

[0226] The amino acid sequence of a 121P1F1 protein as shown in FIG. 2 or FIG. 3 can be analyzed to select specific regions of the 121P1F1 protein for generating antibodies. For example, hydrophobicity and hydrophilicity analyses of a 121P1F1 amino acid sequence are used to identify hydrophilic regions in the 121P1F1 structure. Regions of a 121P1F1 protein that show immunogenic structure, as well as other regions and domains, can readily be identified using various other methods known in the art, such as Chou-Fasman, Gamier-Robson, Kyte-Doolittle, Eisenberg, Karplus-Schultz or Jameson-Wolf analysis. Hydrophilicity profiles can be generated using the method of Hopp, T. P. and Woods, K. R., 1981, Proc. Natl. Acad. Sci. U.S.A. 78::3824-3828. Hydropathicity profiles can be generated using the method of Kyte, J. and Doolittle, R. F., 1982, J. Mol. Biol. 157:105-132. Percent (%) Accessible Residues profiles can be generated using the method of Janin J., 1979, Nature 277:491-492. Average Flexibility profiles can be generated using the method of Bhaskaran R., Ponnuswamy P. K., 1988, Int. J. Pept. Protein Res. 32:242-255. Beta-turn profiles can be generated using the method of Deleage, G., Roux B., 1987, Protein Engineering 1:289-294. Thus, each region identified by any of these programs or methods is within the scope of the present invention. Methods for the generation of 121P1F1 antibodies are further illustrated by way of the examples provided herein. Methods for preparing a protein or polypeptide for use as an immunogen are well known in the art. Also well known in the art are methods for preparing immunogenic conjugates of a protein with a carrier, such as BSA, KLH or other carrier protein. In some circumstances, direct conjugation using, for example, carbodiimide reagents are used; in other instances linking reagents such as those supplied by Pierce Chemical Co., Rockford, Ill., are effective. Administration of a 121P1F1 immunogen is often conducted by injection over a suitable time period and with use of a suitable adjuvant, as is understood in the art. During the immunization schedule, titers of antibodies can be taken to determine adequacy of antibody formation.

[0227] 121P1F1 monoclonal antibodies can be produced by various means well known in the art. For example, immortalized cell lines that secrete a desired monoclonal antibody are prepared using the standard hybridoma technology of Kohler and Milstein or modifications that immortalize antibody-producing B cells, as is generally known. Immortalized cell lines that secrete the desired antibodies are screened by immunoassay in which the antigen is a 121P1F1-related protein. When the appropriate immortalized cell culture is identified, the cells can be expanded and antibodies produced either from in vitro cultures or from ascites fluid.

[0228] The antibodies or fragments of the invention can also be produced, by recombinant means. Regions that bind specifically to the desired regions of a 121P1F1 protein can also be produced in the context of chimeric or complementarity determining region (CDR) grafted antibodies of multiple species origin. Humanized or human 121P1F1 antibodies can also be produced, and are preferred for use in therapeutic contexts. Methods for humanizing murine and other non-human antibodies, by substituting one or more of the non-human antibody CDRs for corresponding human antibody sequences, are well known (see for example, Jones et al., 1986, Nature 321: 522-525; Riechmann et al., 1988, Nature 332: 323-327; Verhoeyen et al., 1988, Science 239): 1534-1536). See also, Carter et al., 1993, Proc. Natl. Acad. Sci. USA 89: 4285 and Sims et al, 1993, J. Immunol. 151: 2296.

[0229] Methods for producing fully human monoclonal antibodies include phage display and transgenic methods (for review, see Vaughan et al., 1998, Nature Biotechnology 16: 535-539). Fully human 121P1F1 monoclonal antibodies can be generated using cloning technologies employing large human Ig gene combinatorial libraries (i.e., phage display) (Griffiths and Hoogenboom, Building an in vitro immune system: human antibodies from phage display libraries. In: Protein Engineering of Antibody Molecules for Prophylacti and Therapeutic Applications in Man, Clark, M. (Ed.), Nottingham Academic, pp 45-64 (1993); Burton and Barbas, Human Antibodies from combinatorial libraries. Id., pp 65-82). Fully human 121P1F1 monoclonal antibodies can also be produced using transgenic mice engineered to contain human immunoglobulin gene loci as described in PCT Patent Application WO98/24893, Kucherlapati and Jakobovits et al., published Dec. 3, 1997 (see also, Jakobovits, 1998, Exp. Opin. Invest. Drugs 7(4): 607-614; U.S. Pat. Nos. 6,162,963 issued Dec. 19, 2000; 6,150,584 issued Nov. 12, 2000; and, 6,114598 issued Sep. 5, 2000). This method avoids the in vitro manipulation required with phage display technology and efficiently produces high affinity authentic human antibodies.

[0230] Reactivity of 121P1F1 antibodies with an 121P1F 1-related protein can be established by a number of well known means, including Western blot, immunoprecipitation, ELISA, and FACS analyses using, as appropriate, 121P1F1-related proteins, 121P1F1-expressing cells or extracts thereof. A 121 IP1F1 antibody or fragment thereof can be labeled with a detectable marker or conjugated to a second molecule. Suitable detectable markers include, but are not limited to, a radioisotope, a fluorescent compound, a bioluminescent compound, chemiluminescent compound, a metal chelator or an enzyme. Further, bi-specific antibodies specific for two or more 121P1F1 epitopes are generated using methods generally known in the art. Homodimeric antibodies can also be generated by cross-linking techniques known in the art (e.g., Wolff et al., Cancer Res. 53: 2560-2565).

[0231] V.) 121P1F1 Cellular Immune Responses

[0232] The mechanism by which T cells recognize antigens has been delineated. Efficacious peptide epitope vaccine compositions of the invention induce a therapeutic or prophylactic immune responses in very broad segments of the world-wide population. For an understanding of the value and efficacy of compositions of the invention that induce cellular immune responses, a brief review of immunology-related technology is provided.

[0233] A complex of an HLA molecule and a peptidic antigen acts as the ligand recognized by HLA-restricted T cells (Buus, S. et al., Cell 47:1071, 1986; Babbitt, B. P. et al., Nature 317:359, 1985; Townsend, A. and Bodmer, H., Annu. Rev. Immunol. 7:601, 1989; Germain, R. N., Annu. Rev. Immunol. 11:403, 1993). Through the study of single amino acid substituted antigen analogs and the sequencing of endogenously bound, naturally processed peptides, critical residues that correspond to motifs required for specific binding to HLA antigen molecules have been identified and are set forth in Table IV (see also, e.g., Southwood, et al., J. Immunol. 160:3363, 1998; Rammensee, et al., Immunogenetics 41:178, 1995; Rammensee et al., SYFPEITHI, access via World Wide Web at URL syfpeithi.bmi-heidelberg.com/; Sette, A. and Sidney, J. Curr. Opin. Immunol. 10:478, 1998; Engelhard, V. H., Curr. Opin. Immunol. 6:13, 1994; Sette, A. and Grey, H. M., Curr. Opin. Immunol. 4:79, 1992; Sinigaglia, F. and Hammer, J. Curr. Biol. 6:52, 1994; Ruppert et al., Cell 74:929-937, 1993; Kondo et al., J. Immunol. 155:4307-4312, 1995; Sidney et al., J. Immunol. 157:3480-3490, 1996; Sidney et al., Human Immunol. 45:79-93, 1996; Sette, A. and Sidney, J. Immunogenetics 1999 November; 50(3-4):201-12, Review).

[0234] Furthermore, x-ray crystallographic analyses of HLA-peptide complexes have revealed pockets within the peptide binding cleft/groove of HLA molecules which accommodate, in an allele-specific mode, residues borne by peptide ligands; these residues in turn determine the HLA binding capacity of the peptides in which they are present. (See, e.g., Madden, D. R. Annu. Rev. Immunol. 13:587, 1995; Smith, et al., Immunity 4:203, 1996; Fremont et al., Immunity 8:305, 1998; Stem et al., Structure 2:245, 1994; Jones, E. Y. Curr. lapin. Immunol. 9:75, 1997; Brown, J. H. et al., Nature 364:33, 1993; Guo, H. C. et al., Proc. Natl. Acad. Sci. USA 90:8053, 1993; Guo, H. C. et al., Nature 360:364, 1992; Silver, M. L. et al., Nature 360:367, 1992; Matsumura, M. et al., Science 257:927, 1992; Madden et al., Cell 70:1035, 1992; Fremont, D. H. et al., Science 257:919, 1992; Saper, M. A., Bjorkman, P. J. and Wiley, D. C., J. Mol. Biol. 219:277, 1991.)

[0235] Accordingly, the definition of class I and class II allele-specific HLA binding motifs, or class I or class II supermotifs allows identification of regions within a protein that are correlated with binding to particular HLA antigen(s).

[0236] Thus, by a process of HLA motif identification, candidates for epitope-based vaccines have been identified; such candidates can be further evaluated by HLA-peptide binding assays to determine binding affinity and/or the time period of association of the epitope and its corresponding HLA molecule. Additional confirmatory work can be performed to select, amongst these vaccine candidates, epitopes with preferred characteristics in terms of population coverage, and/or immunogenicity.

[0237] Various strategies can be utilized to evaluate cellular immunogenicity, including:

[0238] 1) Evaluation of primary T cell cultures from normal individuals (see, e.g., Wentworth, P. A. et al., Mol. Immunol. 32:603, 1995; Celis, E. et al., Proc. Natl. Acad. Sci. USA 91:2105, 1994; Tsai, V. et al., J. Immunol. 158:1796, 1997; Kawashima, I. et al., Human Immunol. 59:1, 1998). This procedure involves the stimulation of peripheral blood lymphocytes (PBL) from normal subjects with a test peptide in the presence of antigen presenting cells in vitro over a period of several weeks. T cells specific for the peptide become activated during this time and are detected using, e.g., a lymphokine- or ⁵¹Cr-release assay involving peptide sensitized target cells.

[0239] 2) Immunization of HLA transgenic mice (see, e.g., Wentworth, P. A. et al., J. Immunol. 26:97, 1996; Wentworth, P. A. et al., Int. Immunol. 8:651, 1996; Alexander, J. et al., J. Immunol. 159:4753, 1997). For example, in such methods peptides in incomplete Freund's adjuvant are administered subcutaneously to HLA transgenic mice. Several weeks following immunization, splenocytes are removed and cultured in vitro in the presence of test peptide for approximately one week. Peptide-specific T cells are detected using, e.g., a ⁵¹Cr-release assay involving peptide sensitized target cells and target cells expressing endogenously generated antigen.

[0240] 3) Demonstration of recall T cell responses from immune individuals who have been either effectively vaccinated and/or from chronically ill patients (see, e.g., Rehermann, B. et al., J. Exp. Med. 181:1047, 1995; Doolan, D. L. et al., Immunity 7:97, 1997; Bertoni, R. et al., J. Clin. Invest. 100:503, 1997; Threlkeld, S. C. et al., J. Immunol. 159:1648, 1997; Diepolder, H. M. et al., J. Virol. 71:6011, 1997). Accordingly, recall responses are detected by culturing PBL from subjects that have been exposed to the antigen due to disease and thus have generated an immune response “naturally”, or from patients who were vaccinated against the antigen. PBL from subjects are cultured in vitro for 1-2 weeks in the presence of test peptide plus antigen presenting cells (APC) to allow activation of “memory” T cells, as compared to “naive” T cells. At the end of the culture period, T cell activity is detected using assays including ⁵¹Cr release involving peptide-sensitized targets, T cell proliferation, or lymphokine release.

[0241] VI.) 121P1F1 Transgenic Animals

[0242] Nucleic acids that encode a 121P1F1-related protein can also be used to generate either transgenic animals or “knock out” animals that, in turn, are useful in the development and screening of therapeutically useful reagents. In accordance with established techniques, cDNA encoding 121P1F1 can be used to clone genomic DNA that encodes 121P1F1. The cloned genomic sequences can then be used to generate transgenic animals containing cells that express DNA that encode 121P1F1. Methods for generating transgenic animals, particularly animals such as mice or rats, have become conventional in the art and are described, for example, in U.S. Pat. Nos. 4,736,866 issued Apr. 12, 1988, and 4,870,009 issued Sep. 26, 1989. Typically, particular cells would be targeted for 121P1F1 transgene incorporation with tissue-specific enhancers.

[0243] Transgenic animals that include a copy of a transgene encoding 121P1F1 can be used to examine the effect of increased expression of DNA that encodes 121P1F1. Such animals can be used as tester animals for reagents thought to confer protection from, for example, pathological conditions associated with its overexpression. In accordance with this aspect of the invention, an animal is treated with a reagent and a reduced incidence of a pathological condition, compared to untreated animals that bear the transgene, would indicate a potential therapeutic intervention for the pathological condition.

[0244] Alternatively, non-human homologues of 121P1F1 can be used to construct a 121P1F1 “knock out” animal that has a defective or altered gene encoding 121P1F1 as a result of homologous recombination between the endogenous gene encoding 121P1F1 and altered genomic DNA encoding 121P1F1 introduced into an embryonic cell of the animal. For example, cDNA that encodes 121P1F 1 can be used to clone genomic DNA encoding 121P1F1 in accordance with established techniques. A portion of the genomic DNA encoding 121P1F1 can be deleted or replaced with another gene, such as a gene encoding a selectable marker that can be used to monitor integration. Typically, several kilobases of unaltered flanking DNA (both at the 5′ and 3′ ends) are included in the vector (see, e.g., Thomas and Capecchi, Cell, 51:503 (1987) for a description of homologous recombination vectors). The vector is introduced into an embryonic stem cell line (e.g., by electroporation) and cells in which the introduced DNA has homologously recombined with the endogenous DNA are selected (see, e.g., Li et al., Cell, 69:915 (1992)). The selected cells are then injected into a blastocyst of an animal (e.g., a mouse or rat) to form aggregation chimeras (see, e.g., Bradley, in Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, E. J. Robertson, ed. (IRL, Oxford, 1987), pp. 113-152). A chimeric embryo can then be implanted into a suitable pseudopregnant female foster animal, and the embryo brought to term to create a “knock out” animal. Progeny harboring the homologously recombined DNA in their germ cells can be identified by standard techniques and used to breed animals in which all cells of the animal contain the homologously recombined DNA. Knock out animals can be characterized, for example, for their ability to defend against certain pathological conditions or for their development of pathological conditions due to absence of a 121P1F1 polypeptide.

[0245] VII.) Methods for the Detection of 121P1F1

[0246] Another aspect of the present invention relates to methods for detecting 121P1F1 polynucleotides and 121P1F1-related proteins, as well as methods for identifying a cell that expresses 121P1F1. The expression profile of 121P1F1 makes it a diagnostic marker for metastasized disease. Accordingly, the status of 121P1F1 gene products provides information useful for predicting a variety of factors including susceptibility to advanced stage disease, rate of progression, and/or tumor aggressiveness. As discussed in detail herein, the status of 121P1F1 gene products in patient samples can be analyzed by a variety protocols that are well known in the art including immunohistochemical analysis, the variety of Northern blotting techniques including in situ hybridization, RT-PCR analysis (for example on laser capture micro-dissected samples), Western blot analysis and tissue array analysis.

[0247] More particularly, the invention provides assays for the detection of 121P1F1 polynucleotides in a biological sample, such as serum, bone, prostate, and other tissues, urine, semen, cell preparations, and the like. Detectable 121P1F1 polynucleotides include, for example, a 121P1F1 gene or fragment thereof, 121P1F1 mRNA, alternative splice variant 121P1F1 mRNAs, and recombinant DNA or RNA molecules that contain a 121P1F1 pclynucleotide. A number of methods for amplifying and/or detecting the presence of 121P1F1 polynucleotides are well known in the art and can be employed in the practice of this aspect of the invention.

[0248] In one embodiment, a method for detecting an 121P1F1 mRNA in a biological sample comprises producing cDNA from the sample by reverse transcription using at least one primer; amplifying the cDNA so produced using an 121P1F1 polynucleotides as sense and antisense primers to amplify 121P1F1 cDNAs therein; and detecting the presence of the amplified 121P1F1 cDNA. Optionally, the sequence of the amplified 121P1F1 cDNA can be determined.

[0249] In another embodiment, a method of detecting a 121P1F1 gene in a biological sample comprises first isolating genomic DNA from the sample; amplifying the isolated genomic DNA using 121P1F1 polynucleotides as sense and antisense primers; and detecting the presence of the amplified 121P1F1 gene. Any number of appropriate sense and antisense probe combinations can be designed from a 121P1FI nucleotide sequence (see, e.g., FIG. 2) and used for this purpose.

[0250] The invention also provides assays for detecting the presence of an 121P1F1 protein in a tissue or other biological sample such as serum, semen, bone, prostate, urine, cell preparations, and the like. Methods for detecting a 121P1F1-related protein are also well known and include, for example, immunoprecipitation, immunohistochemical analysis, Western blot analysis, molecular binding assays, ELISA, ELIFA and the like. For example, a method of detecting the presence of a 121P1F1-related protein in a biological sample comprises first contacting the sample with a 121P1F1 antibody, a 121P1F1-reactive fragment thereof, or a recombinant protein containing an antigen binding region of a 121P1F1 antibody; and then detecting the binding of 121P1F1-related protein in the sample.

[0251] Methods for identifying a cell that expresses 121P1F1 are also within the scope of the invention. In one embodiment, an assay for identifying a cell that expresses a 121P1F1 gene comprises detecting the presence of 121P1F1 mRNA in the cell. Methods for the detection of particular mRNAs in cells are well known and include, for example, hybridization assays using complementary DNA probes (such as in situ hybridization using labeled 121P1F1 riboprobes, Northern blot and related techniques) and various nucleic acid amplification assays (such as RT-PCR using complementary primers specific for 121P1F1, and other amplification type detection methods, such as, for example, branched DNA, SISBA, TMA and the like). Alternatively, an assay for identifying a cell that expresses a 121P1F1 gene comprises detecting the presence of 121P1F1-related protein in the cell or secreted by the cell. Various methods for the detection of proteins are well known in the art and are employed for the detection of 121P1F1-related proteins and cells that express 121P1F1-related proteins.

[0252] 121P1F1 expression analysis is also useful as a tool for identifying and evaluating agents that modulate 121P1F1 gene expression. For example, 121P1F1 expression is significantly upregulated in prostate cancer, and is expressed in cancers of the tissues listed in Table I. Identification of a molecule or biological agent that inhibits 121P1F1 expression or over-expression in cancer cells is of therapeutic value. For example, such an agent can be identified by using a screen that quantifies 121P1F1 expression by RT-PCR, nucleic acid hybridization or antibody binding.

[0253] VIII.) Methods for Monitoring the Status of 121P1F1-Related Genes and Their Products

[0254] Oncogenesis is known to be a multistep process where cellular growth becomes progressively dysregulated and cells progress from a normal physiological state to precancerous and then cancerous states (see, e.g., Alers et al., Lab Invest. 77(5): 437-438 (1997) and Isaacs et al., Cancer Surv. 23: 19-32 (1995)). In this context, examining a biological sample for evidence of dysregulated cell growth (such as aberrant 121P1F1 expression in cancers) allows for early detection of such aberrant physiology, before a pathologic n state such as cancer has progressed to a stage that therapeutic options are more limited and or the prognosis is worse. In such examinations, the status of 121P1F1 in a biological sample of interest can be compared, for example, to the status of 121P1F1 in a corresponding normal sample (e.g. a sample from that individual or alternatively another individual that is not affected by a pathology). An alteration in the status of 121P1F1 in the biological sample (as compared to the normal sample) provides evidence of dysregulated cellular growth. In addition to using a biological sample that is not affected by a pathology as a normal sample, one can also use a predetermined normative value such as a predetermined normal level of mRNA expression (see, e.g., Grever et al., J. Comp. Neurol. Dec. 9, 1996; 376(2): 306-14 and U.S. Pat. No. 5,837,501) to compare 121P1F1 status in a sample.

[0255] The term “status” in this context is used according to its art accepted meaning and refers to the condition or state of a gene and its products. Typically, skilled artisans use a number of parameters to evaluate the condition or state of a gene and its products. These include, but are not limited to the location of expressed gene products (including the location of 121P1F1 expressing cells) as well as the level, and biological activity of expressed gene products (such as 121P1F1 mRNA, polynucleotides and polypeptides). Typically, an alteration in the status of 121P1F1 comprises a change in the location of 121P1F1 and/or 121P1F1 expressing cells and/or an increase in 121P1F1 mRNA and/or protein expression.

[0256] 121P1F1 status in a sample can be analyzed by a number of means well known in the art, including without limitation, immunohistochemical analysis, in situ hybridization, RT-PCR analysis on laser capture micro-dissected samples, Western blot analysis, and tissue array analysis. Typical protocols for evaluating the status of a 121P1F1 gene and gene products are found, for example in Ausubel et al. eds., 1995, Current Protocols In Molecular Biology, Units 2 (Northern Blotting), 4 (Southern Blotting), 15 (Immunoblotting) and 18 (PCR Analysis). Thus, the status of 121P1F1 in a biological sample is evaluated by various methods utilized by skilled artisans including, but not limited to genomic Southern analysis (to examine, for example perturbations in a 121P1F1 gene), Northern analysis and/or PCR analysis of 121P1F1 mRNA (to examine, for example alterations in the polynucleotide sequences or expression levels of 121P1F1 mRNAs), and, Western and/or immunohistochemical analysis (to examine, for example alterations in polypeptide sequences, alterations in polypeptide localization within a sample, alterations in expression levels of 121P1F1 proteins and/or associations of 121P1F1 proteins with polypeptide binding partners). Detectable 121P1F1 polynucleotides include, for example, a 121P1F1 gene or fragment thereof, 121P1F1 mRNA, alternative splice variants, 121P1F1 mRNAs, and recombinant DNA or RNA molecules containing a 121P1F1 polynucleotide.

[0257] The expression profile of 121P1F1 makes it a diagnostic marker for local and/or metastasized disease, and provides information on the growth or oncogenic potential of a biological sample. In particular, the status of 121P1F1 provides information useful for predicting susceptibility to particular disease stages, progression, and/or tumor aggressiveness. The invention provides methods and assays for determining 121P1F1 status and diagnosing cancers that express 121P1F1, such as cancers of the tissues listed in Table I. For example, because 121P1F1 mRNA is so highly expressed in prostate and other cancers relative to normal prostate tissue, assays that evaluate the levels of 121P1F1 mRNA transcripts or proteins in a biological sample can be used to diagnose a disease associated with 121P1F1 dysregulation, and can provide prognostic information useful in defining appropriate therapeutic options.

[0258] The expression status of 121P1F1 provides information including the presence, stage and location of dysplastic, precancerous and cancerous cells, predicting susceptibility to various stages of disease, and/or for gauging tumor aggressiveness. Moreover, the expression profile makes it useful as an imaging reagent for metastasized disease. Consequently, an aspect of the invention is directed to the various molecular prognostic and diagnostic methods for examining the status of 121P1F1 in biological samples such as those from individuals suffering from, or suspected of suffering from a pathology characterized by dysregulated cellular growth, such as cancer.

[0259] As described above, the status of 121P1F1 in a biological sample can be examined by a number of well-known procedures in the art. For example, the status of 121P1F1 in a biological sample taken from a specific location in the body can be examined by evaluating the sample for the presence or absence of 121P1F1 expressing cells (e.g. those that express 121P1F1 mRNAs or proteins). This examination can provide evidence of dysregulated cellular growth, for example, when 121P1F1-expressing cells are found in a biological sample that does not normally contain such cells (such as a lymph node), because such alterations in the status of 121P1F1 in a biological sample are often associated with dysregulated cellular growth. Specifically, one indicator of dysregulated cellular growth is the metastases of cancer cells from an organ of origin (such as the prostate) to a different area of the body (such as a lymph node). In this context, evidence of dysregulated cellular growth is important for example because occult lymph node metastases can be detected in a substantial proportion of patients with prostate cancer, and such metastases are associated with known predictors of disease progression (see, e.g., Murphy et al., Prostate 42(4): 315-317 (2000);Su et al., Semin. Surg. Oncol. 18(1): 17-28 (2000) and Freeman etal., J Urol 1995 Aug 154(2 Pt 1):474-8).

[0260] In one aspect, the invention provides methods for monitoring 121P1F1 gene products by determining the status of 121P1F1 gene products expressed by cells from an individual suspected of having a disease associated with dysregulated cell growth (such as hyperplasia or cancer) and then comparing the status so determined to the status of 121P1F1 gene products in a corresponding normal sample. The presence of aberrant 121P1F1 gene products in the test sample relative to the normal sample provides an indication of the presence of dysregulated cell growth within the cells of the individual.

[0261] In another aspect, the invention provides assays useful in determining the presence of cancer in an individual, comprising detecting a significant increase in 121P1F1 mRNA or protein expression in a test cell or tissue sample relative to expression levels in the corresponding normal cell or tissue. The presence of 121P1F1 mRNA can, for example, be evaluated in tissue samples including but not limited to those listed in Table I. The presence of significant 121P1F1 expression in any of these tissues is useful to indicate the emergence, presence and/or severity of a cancer, since the corresponding normal tissues do not express 121P1F1 mRNA or express it at lower levels.

[0262] In a related embodiment, 121P1F1 status is determined at the protein level rather than at the nucleic acid level. For example, such a method comprises determining the level of 121P1F1 protein expressed by cells in a test tissue sample and comparing the level so determined to the level of 121P1F1 expressed in a corresponding normal sample. In one embodiment, the presence of 121P1F1 protein is evaluated, for example, using immunohistochemical methods. 121P1F1 antibodies or binding partners capable of detecting 121P1F1 protein expression are used in a variety of assay formats well known in the art for this purpose.

[0263] In a further embodiment, one can evaluate the status of 121P1F1 nucleotide and amino acid sequences in a biological sample in order to identify perturbations in the structure of these molecules. These perturbations can include insertions, deletions, substitutions and the like. Such evaluations are useful because perturbations in the nucleotide and amino acid sequences are observed in a large number of proteins associated with a growth dysregulated phenotype (see, e.g., Marrogi et al., 1999, J. Cutan. Pathol. 26(8):369-378). For example, a mutation in the sequence of 121P1F1 may be indicative of the presence or promotion of a tumor. Such assays therefore have diagnostic and predictive value where a mutation in 121P1F1 indicates a potential loss of function or increase in tumor growth.

[0264] A wide variety of assays for observing perturbations in nucleotide and amino acid sequences are well known in the art. For example, the size and structure of nucleic acid or amino acid sequences of 121P1F1 gene products are observed by the Northern, Southern, Western, PCR and DNA sequencing protocols discussed herein. In addition, other methods for observing perturbations in nucleotide and amino acid sequences such as single strand conformation polymorphism analysis are well known in the art (see, e.g., U.S. Pat. Nos. 5,382,510 issued Sep. 7, 1999, and 5,952,170 issued Jan. 17, 1995).

[0265] Additionally, one can examine the methylation status of a 121P1F1 gene in a biological sample. Aberrant delmethylation and/or hypermethylation of CpG islands in gene 5′ regulatory regions frequently occurs in immortalized and transformed cells, and can result in altered expression of various genes. For example, promoter hypermethylation of the pi-class glutathione S-transferase (a protein expressed in normal prostate but not expressed in>90% of prostate carcinomas) appears to permanently silence transcription of this gene and is the most frequently detected genomic alteration in prostate carcinomas (De Marzo et al., Am. J. Pathol. 155(6): 1985-1992 (1999)). In addition, this alteration is present in at least 70% of cases of high-grade prostatic intraepithelial neoplasia (PIN) (Brooks et al., Cancer Epidemiol. Biomarkers Prev., 1998, 7:531-536). In another example, expression of the LAGE-1 tumor specific gene (which is not expressed in normal prostate but is expressed in 25-50% of prostate cancers) is induced by deoxy-azacytidine in lymphoblastoid cells, suggesting that tumoral expression is due to demethylation (Lethe et al., Int. J. Cancer 76(6): 903-908 (1998)). A variety of assays for examining methylation status of a gene are well known in the art. For example, one can utilize, in Southern hybridization approaches, methylation-sensitive restriction enzymes that cannot cleave sequences that contain methylated CpG sites to assess the methylation status of CpG islands. In addition, MSP (methylation specific PCR) can rapidly profile the methylation status of all the CpG sites present in a CpG island of a given gene. This procedure involves initial modification of DNA by sodium bisulfite (which will convert all unmethylated cytosines to uracil) followed by amplification using primers specific for methylated versus unmethylated DNA. Protocols involving methylation interference can also be found for example in Current Protocols In Molecular Biology, Unit 12, Frederick M. Ausubel et al. eds., 1995.

[0266] Gene amplification is an additional method for assessing the status of 121P1F1. Gene amplification is measured in a sample directly, for example, by conventional Southern blotting or Northern blotting to quantitate the transcription of mRNA (Thomas, 1980, Proc. Natl. Acad. Sci. USA, 77:5201-5205), dot blotting (DNA analysis), or in situ hybridization, using an appropriately labeled probe, based on the sequences provided herein. Alternatively, antibodies are employed that recognize specific duplexes, including DNA duplexes, RNA duplexes, and DNA-RNA hybrid duplexes or DNA-protein duplexes. The antibodies in turn are labeled and the assay carried out where the duplex is bound to a surface, so that upon the formation of duplex on the surface, the presence of antibody bound to the duplex can be detected.

[0267] Biopsied tissue or peripheral blood can be conveniently assayed for the presence of cancer cells using for example, Northern, dot blot or RT-PCR analysis to detect 121P1F1 expression. The presence of RT-PCR amplifiable 121P1F1 mRNA provides an indication of the presence of cancer. RT-PCR assays are well known in the art. FT-PCR detection assays for tumor cells in peripheral blood are currently being evaluated for use in the diagnosis and management of a number of human solid tumors. In the prostate cancer field, these include RT-PCR assays for the detection of cells expressing PSA and PSM (Verkaik et al, 1997, Urol. Res. 25:373-384; Ghossein et al, 1995, J. Clin. Oncol. 13:1195-2000; Heston et al, 1995, Clin. Chem. 41:1687-1688).

[0268] A further aspect of the invention is an assessment of the susceptibility that an individual has for developing cancer. In one embodiment, a method for predicting susceptibility to cancer comprises detecting 121P1F1 mRNA or 121P1F1 protein in a tissue sample, its presence indicating susceptibility to cancer, wherein the degree of 121P1F1 mRNA expression correlates to the degree of susceptibility. In a specific embodiment, the presence of 121P1F1 in prostate or other tissue is examined, with the presence of 121P1F1 in the sample providing an indication of prostate cancer susceptibility (or the emergence or existence of a prostate tumor). Similarly, one can evaluate the integrity 121P1F1 nucleotide and amino acid sequences in a biological sample, in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like. The presence of one or more perturbations in 121P1F1 gene products in the sample is an indication of cancer susceptibility (or the emergence or existence of a tumor).

[0269] The invention also comprises methods for gauging tumor aggressiveness. In one embodiment, a method for gauging aggressiveness of a tumor comprises determining the level of 121P1F1 mRNA or 121P1F1 protein expressed by tumor cells, comparing the level so determined to the level of 121P1F1 mRNA or 121P1F1 protein expressed in a corresponding normal tissue taken from the same individual or a normal tissue reference sample, wherein the degree of 121P1F1 mRNA or 121P1F1 protein expression in the tumor sample relative to the normal sample indicates the degree of aggressiveness. In a specific embodiment, aggressiveness of a tumor is evaluated by determining the extent to which 121P1F1 is expressed in the tumor cells, with higher expression levels indicating more aggressive tumors. Another embodiment is the evaluation of the integrity of 121P1F1 nucleotide and amino acid sequences in a biological sample, in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like. The presence of one or more perturbations indicates more aggressive tumors.

[0270] Another embodiment of the invention is directed to methods for observing the progression of a malignancy in an individual over time. In one embodiment, methods for observing the progression of a malignancy in an individual over time comprise determining the level of 121P1F1 mRNA or 121P1F1 protein expressed by cells in a sample of the tumor, comparing the level so determined to the level of 121P1F1 mRNA or 121P1F1 protein expressed in an equivalent tissue sample taken from the same individual at a different time, wherein the degree of 121P1F1 mRNA or 121P1F1 protein expression in the tumor sample over time provides information on the progression of the cancer. In a specific embodiment, the progression of a cancer is evaluated by determining 121P1F1 expression in the tumor cells over time, where increased expression over time indicates a progression of the cancer. Also, one can evaluate the integrity 121P1F1 nucleotide and amino acid sequences in a biological sample in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like, where the presence of one or more perturbations indicates a progression of the cancer.

[0271] The above diagnostic approaches can be combined with any one of a wide variety of prognostic and diagnostic protocols known in the art. For example, another embodiment of the invention is directed to methods for observing a coincidence between the expression of 121P1F1 gene and 121P1F1 gene products (or perturbations in 121P1F1 gene and 121P1F1 gene products) and a factor that is associated with malignancy, as a means for diagnosing and prognosticating the status of a tissue sample. A wide variety of factors associated with malignancy can be utilized, such as the expression of genes associated with malignancy (e.g. PSA, PSCA and PSM expression for prostate cancer etc.) as well as gross cytological observations (see, e.g., Bocking et al, 1984, Anal. Quant. Cytol. 6(2):74-88; Epstein, 1995, Hum. Pathol. 26(2):223-9; Thorson et al., 1998, Mod. Pathol. 11 ((6):543-51; Baisden et al., 1999, Am. J. Surg. Pathol. 23(8):918-24). Methods for observing a coincidence between the expression of 121P1F1 gene and 121P1F1 gene products (or perturbations in 121P1F1 gene and 121P1F1 gene products) and another factor that is associated with malignancy are useful, for example, because the presence of a set of specific factors that coincide with disease provides information crucial for diagnosing and prognosticating the status of a tissue sample.

[0272] In one embodiment, methods for observing a coincidence between the expression of 121P1F1 gene and 121P1F1 gene products (orperturbations in 121P1F1 gene and 121P1F1 gene products) and another factor associated with malignancy entails detecting the overexpression of 121P1F 1 mRNA or protein in a tissue sample, detecting the overexpression of PSA mRNA or protein in a tissue sample (or PSCA or PSM expression), and observing a coincidence of 121P1F1 mRNA or protein and PSA mRNA or protein overexpression (or PSCA or PSM expression). In a specific embodiment, the expression of 121P1F1 and PSA mRNA in prostate tissue is examined, where the coincidence of 121P1F1 and PSA mRNA overexpression in the sample indicates the existence of prostate cancer, prostate cancer susceptibility or the emergence or status of a prostate tumor.

[0273] Methods for detecting and quantifying the expression of 121P1F1 mRNA or protein are described herein, and standard nucleic acid and protein detection and quantification technologies are well known in the art. Standard methods for the detection and quantification of 121P1F1 mRNA include in situ hybridization using labeled 121P1F1 riboprobes, Northern blot and related techniques using 121P1F1 polynucleotide probes, RT-PCR analysis using primers specific for 121P1F1, and other amplification type detection methods, such as, for example, branched DNA, SISBA, TMA and the like. In a specific embodiment, semi-quantitative RT-PCR is used to detect and quantify 121P1F1 mRNA expression. Any number of primers capable of amplifying 121P1F1 can be used for this purpose, including but not limited to the various primer sets specifically described herein. In a specific embodiment, polyclonal or monoclonal antibodies specifically reactive with the wild-type 121P1F1 protein can be used in an immunohistochemical assay of biopsied tissue.

[0274] IX.) Identification of Molecules That Interact With 121P1F1

[0275] The 121P1F1 protein and nucleic acid sequences disclosed herein allow a skilled artisan to identify proteins, small molecules and other agents that interact with 121P1F1, as well as pathways activated by 121P1F1 via any one of a variety of art accepted protocols. For example, one can utilize one of the so-called interaction trap systems (also referred to as the “two-hybrid assay”). In such systems, molecules interact and reconstitute a transcription factor which directs expression of a reporter gene, whereupon the expression of the reporter gene is assayed. Other systems identify protein-protein interactions in vivo through reconstitution of a eukaryotic transcriptional activator, see, e.g., U.S. Pat. Nos. 5,955,280 issued Sep. 21, 1999, 5,925,523 issued Jul. 20, 1999, 5,846,722 issued Dec. 8, 1998 and 6,004,746 issued Dec. 21, 1999. Algorithms are also available in the art for genome-based predictions of protein function (see, e.g., Marcotte, et al., Nature 402: 4 November 1999, 83-86).

[0276] Alternatively one can screen peptide libraries to identify molecules that interact with 121P1F1 protein sequences. In such methods, peptides that bind to 121P1F1 are identified by screening libraries that encode a random or controlled collection of amino acids. Peptides encoded by the libraries are expressed as fusion proteins of bacteriophage coat proteins, the bacteriophage particles are then screened against the 121P1F1 protein(s).

[0277] Accordingly, peptides having a wide variety of uses, such as therapeutic, prognostic or diagnostic reagents, are thus identified without any prior information on the structure of the expected ligand or receptor molecule. Typical peptide libraries and screening methods that can be used to identify molecules that interact with 121P1F1 protein sequences are disclosed for example in U.S. Pat. Nos. 5,723,286 issued Mar. 3, 1998 and 5,733,731 issued Mar. 31, 1998.

[0278] Alternatively, cell lines that express 121P1F1 are used to identify protein-protein interactions mediated by 121P1F1. Such interactions can be examined using immunoprecipitation techniques (see, e.g., Hamilton 3.J., et al. Biochem. Biophys. Res. Commun. 1999, 261:646-51). 121P1F1 protein can be immunoprecipitated from 121P1F1-expressing cell lines using anti-121P1F1 antibodies. Alternatively, antibodies against His-tag can be used in a cell line engineered to express fusions of 121P1F1 and a His-tag (vectors mentioned above). The immunoprecipitated complex can be examined for protein association by procedures such as Western blotting, ³⁵S-methionine labeling of proteins, protein microsequencing, silver staining and two-dimensional gel electrophoresis.

[0279] Small molecules and ligands that interact with 121P1F1 can be identified through related embodiments of such screening assays. For example, small molecules can be identified that interfere with protein function, including molecules that interfere with 121P1F1's ability to mediate phosphorylation and de-phosphorylation, interaction with DNA or RNA molecules as an indication of regulation of cell cycles, second messenger signaling or tumorigenesis. Similarly, small molecules that modulate 121P1F1-related ion channel, protein pump, or cell communication functions are identified and used to treat patients that have a cancer that expresses 121P1F1 (see, e.g., Hille, B., Ionic Channels of Excitable Membranes 2^(nd) Ed., Sinauer Assoc., Sunderland, Mass., 1992). Moreover, ligands that regulate 121P1F1 function can be identified based on their ability to bind 121P1F1 and activate a reporter construct. Typical methods are discussed for example in U.S. Pat. No. 5,928,868 issued Jul. 27, 1999, and include methods for forming hybrid ligands in which at least one ligand is a small molecule. In an illustrative embodiment, cells engineered to express a fusion protein of 121P1F1 and a DNA-binding protein are used to co-express a fusion protein of a hybrid ligand/small molecule and a cDNA library transcriptional activator protein. The cells further contain a reporter gene, the expression of which is conditioned on the proximity of the first and second fusion proteins to each other, an event that occurs only if the hybrid ligand binds to target sites on both hybrid proteins. Those cells that express the reporter gene are selected and the unknown small molecule or the unknown ligand is identified. This method provides a means of identifying modulators which activate or inhibit 121P1F1.

[0280] An embodiment of this invention comprises a method of screening for a molecule that interacts with an 121P1F1 amino acid sequence shown in FIG. 2 or FIG. 3, comprising the steps of contacting a population of molecules with a 121P1F1 amino acid sequence, allowing the population of molecules and the 121P1F1 amino acid sequence to interact under conditions that facilitate an interaction, determining the presence of a molecule that interacts with the 121P1F1 amino acid sequence, and then separating molecules that do not interact with the 121P1F1 amino acid sequence from molecules that do. In a specific embodiment, the method further comprises purifying, characterizing and identifying a molecule that interacts with the 121P1F1 amino acid sequence. The identified molecule can be used to modulate a function performed by 121P1F1. In a preferred embodiment, the 121P1F1 amino acid sequence is contacted with a library of peptides.

[0281] X.) Therapeutic Methods and Compositions

[0282] The identification of 121P1F1 as a protein that is normally expressed in a restricted set of tissues, but which is also expressed in prostate and other cancers, opens a number of therapeutic approaches to the treatment of such cancers. As contemplated herein, 121P1F1 functions as a transcription factor involved in activating tumor-promoting genes or repressing genes that block tumorigenesis.

[0283] Accordingly, therapeutic approaches that inhibit the activity of a 121P1F1 protein are useful for patients suffering from a cancer that expresses 121P1F1. These therapeutic approaches generally fall into two classes. One class comprises various methods for inhibiting the binding or association of a 121P1F1 protein with its binding partner or with other proteins. Another class comprises a variety of methods for inhibiting the transcription of a 121P1F1 gene or translation of 121P1F1 mRNA.

[0284] X.A.) Anti-Cancer Vaccines

[0285] The invention provides cancer vaccines comprising a 121P1F1-related protein or 121P1F1-related nucleic acid. In view of the expression of 121P1F1, cancer vaccines prevent and/or treat 121P1F1-expressing cancers with minimal or no effects on non-target tissues. The use of a tumor antigen in a vaccine that generates humoral and/or cell-mediated immune responses as anti-cancer therapy is well known in the art and has been employed in prostate cancer using human PSMA and rodent PAP immunogens (Hodge et al., 1995, Int. J. Cancer 63:231-237; Fong et al., 1997, J. Immunol. 159:3113-3117).

[0286] Such methods can be readily practiced by employing a 121P1F1-related protein, or an 121P1F1-encoding nucleic acid molecule and recombinant vectors capable of expressing and presenting the 121P1F1 immunogen (which typically comprises a number of antibody or T cell epitopes). Skilled artisans understand that a wide variety of vaccine systems for delivery of immunoreactive epitopes are known in the art (see, e.g., Heryln et al., Ann Med 1999 Feb 31(1):66-78; Maruyama et al., Cancer Immunol Immunother 2000 Jun 49(3):123-32) Briefly, such methods of generating an immune response (e.g. humoral and/or cell-mediated) in a mammal, comprise the steps of: exposing the mammal's immune system to an immunoreactive epitope (e.g. an epitope present in a 121P1F1 protein shown in FIG. 3 or analog or homolog thereof) so that the mammal generates an immune response that is specific for that epitope (e.g. generates antibodies that specifically recognize that epitope). In a preferred method, a 121P1F1 immunogen contains a biological motif, see e.g., Tables V-XVIII, XXVI, and XXVII, or a peptide of a size range from 121P1F1 indicated in FIG. 5, FIG. 6, FIG. 7, FIG. 8, and FIG. 9.

[0287] The entire 121P1F1 protein, immunogenic regions or epitopes thereof can be combined and delivered by various means. Such vaccine compositions can include, for example, lipopeptides (e.g., Vitiello, A. et al., J. Clin. Invest. 95:341, 1995), peptide compositions encapsulated in poly(DL-lactide-co-glycolide) (“PLG”) microspheres (see, e.g., Eldridge, et al., Molec. Immunol. 28:287-294, 1991: Alonso et al., Vaccine 12:299-306, 1994; Jones et al., Vaccine 13:675-681, 1995), peptide compositions contained in immune stimulating complexes (ISCOMS) (see, e.g., Takahashi et al., Nature 344:873-875, 1990; Hu et al., Clin Exp Immunol. 113:235-243, 1998), multiple antigen peptide systems (MAPs) (see e.g., Tam, J. P., Proc. Natl. Acad. Sci. U.S.A. 85:5409-5413, 1988; Tam, J. P., J. Immunol. Methods 196:17-32, 1996), peptides formulated as multivalent peptides; peptides for use in ballistic delivery systems, typically crystallized peptides, viral delivery vectors (Perkus, M. E. et al., In: Concepts in vaccine development, Kaufmann, S. H. E., ed., p. 379, 1996; Chakrabarti, S. et al., Nature 320:535, 1986; Hu, S. L. et al., Nature 320:537, 1986; Kieny, M.-P. et al., AIDS Bio/Technology 4:790, 1986; Top, F. H. et al., J. Infect. Dis. 124:148, 1971; Chanda, P. K. et al., Virology 175:535, 1990), particles of viral or synthetic origin (e.g., Kofler, N. etal., J. Immunol. Methods. 192:25, 1996; Eldridge, J. H. et al., Sem. Hematol. 30:16, 1993; Falo, L. D., Jr. et al., Nature Med. 7:649, 1995), adjuvants (Warren, H. S., Vogel, F. R., and Chedid, L. A. Annu. Rev. Immunol. 4:369, 1986; Gupta, R. K. et al., Vaccine 11:293, 1993), liposomes (Reddy, R. et al., J. Immunol. 148:1585, 1992; Rock, K. L., Immunol. Today 17:131, 1996), or, naked or particle absorbed cDNA (Ulmer, J. B. et al., Science 259:1745, 1993; Robinson, H. L., Hunt, L. A., and Webster, R. G., Vaccine 11:957, 1993; Shiver, J. W. et al., In: Concepts in vaccine development, Kaufmann, S. H. E., ed., p. 423, 1996; Cease, K. B., and Berzofsky, J. A., Annu. Rev. Immunol. 12:923, 1994 and Eldridge, J. H. et al., Sem. Hematol. 30:16, 1993). Toxin-targeted delivery technologies, also known as receptor mediated targeting, such as those of Avant Immunotherapeutics, Inc. (Needham, Mass.) may also be used.

[0288] In patients with 121P1F1-associated cancer, the vaccine compositions of the invention can also be used in conjunction with other treatments used for cancer, e.g., surgery, chemotherapy, drug therapies, radiation therapies, etc. including use in combination with immune adjuvants such as IL-2, IL-12, GM-CSF, and the like.

[0289] Cellular Vaccines:

[0290] CTL epitopes can be determined using specific algorithms to identify peptides within 121P1F1 protein that bind corresponding HLA alleles (see e.g., Table IV; Epimer™ and Epimatrix™, Brown University (URL www.brown.edu/Research/TB-HIV_Lab/epimnatrix/epimatrix.html); and, BIMAS, (URL bimas.dcrt.nih.gov/; SYFPEITHI at URL syfpeithi.bmi-heidelberg.com/). In a preferred embodiment, a 121P1F1 immunogen contains one or more amino acid sequences identified using techniques well known in the art, such as the sequences shown in Tables V-XVIII, XXVI, and XXVII or a peptide of 8, 9, 10 or 11 amino acids specified by an HLA Class I motif/supermotif (e.g., Table IV (A), Table IV (D), or Table IV (E)) and/or a peptide of at least 9 amino acids that comprises an HLA Class II motif/supermotif (e.g., Table IV (B) or Table IV (C)). As is appreciated in the art, the HLA Class I binding groove is essentially closed ended so that peptides of only a particular size range can fit into the groove and be bound, generally HLA Class I epitopes are 8, 9, 10, or 11 amino acids long. In contrast, the HLA Class II binding groove is essentially open ended; therefore a peptide of about 9 or more amino acids can be bound by an HLA Class II molecule. Due to the binding groove differences between HLA Class I and II, HLA Class I motifs are length specific, i.e., position two of a Class I motif is the second amino acid in an amino to carboxyl direction of the peptide. The amino acid positions in a Class II motif are relative only to each other, not the overall peptide, i.e., additional amino acids can be attached to the amino and/or carboxyl termini of a motif-bearing sequence. HLA Class II epitopes are often 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids long, or longer than 25 amino acids.

[0291] Antibody-Based Vaccines

[0292] A wide variety of methods for generating an immune response in a mammal are known in the art (for example as the first step in the generation of hybridomas). Methods of generating an immune response in a mammal comprise exposing the mammal's immune system to an immunogenic epitope on a protein (e.g. a 121P1F1 protein) so that an immune response is generated. A typical embodiment consists of a method for generating an immune response to 121P1F1 in a host, by contacting the host with a sufficient amount of at least one 121P1F1 B cell or cytotoxic T-cell epitope or analog thereof; and at least one periodic interval thereafter re-contacting the host with the 121P1F1 B cell or cytotoxic T-cell epitope or analog thereof. A specific embodiment consists of a method of generating an immune response against a 121P1F1-related protein or a man-made multiepitopic peptide comprising: administering 121P1F1 immunogen (e.g. a 121P1F1 protein or a peptide fragment thereof, an 121P1F1 fusion protein or analog etc.) in a vaccine preparation to a human or another mammal. Typically, such vaccine preparations further contain a suitable adjuvant (see, e.g., U.S. Pat. No. 6,146,635) or a universal helper epitope such as a PADRE™ peptide (Epimmune Inc., San Diego, Calif.; see, e.g., Alexander et al., J. Immunol. 2000 164(3); 164(3): 1625-1633; Alexander et al., Immunity 1994 1(9): 751-761 and Alexander et al., Immunol. Res. 1998 18(2): 79-92). An alternative method comprises generating an immune response in an individual against a 121P1F1 immunogen by: administering in vivo to muscle or skin of the individuals body a DNA molecule that comprises a DNA sequence that encodes an 121P1F1 immunogen, the DNA sequence operatively linked to regulatory sequences which control the expression of the DNA sequence; wherein the DNA molecule is taken up by cells, the DNA sequence is expressed in the cells and an immune response is generated against the immunogen (see, e.g., U.S. Pat. No. 5,962,428). Optionally a genetic vaccine facilitator such as anionic lipids; saponins; lectins; estrogenic compounds; hydroxylated lower alkyls; dimethyl sulfoxide; and urea is also administered. In addition, an antiidiotypic antibody can be administered that mimics 121P1F1, in order to generate a response to the target antigen.

[0293] Nucleic Acid Vaccines:

[0294] Vaccine compositions of the invention include nucleic acid-mediated modalities. DNA or RNA that encode protein(s) of the invention can be administered to a patient. Genetic immunization methods can be employed to generate prophylactic or therapeutic humoral and cellular immune responses directed against cancer cells expressing 121P1F1. Constructs comprising DNA encoding a 121P1F1-related protein/immunogen and appropriate regulatory sequences can be injected directly into muscle or skin of an individual, such that the cells of the muscle or skin take-up the construct and express the encoded 121P1F1 protein/immunogen. Alternatively, a vaccine comprises a 121P1F1-related protein. Expression of the 121P1F1-related protein immunogen results in the generation of prophylactic or therapeutic humoral and cellular immunity against cells that bear a 121P1F1 protein. Various prophylactic and therapeutic genetic immunization techniques known in the art can be used (for review, see information and references published al: Internet address www.genweb.com). Nucleic acid-based delivery is described, for instance, in Wolff et. al, Science 247:1465 (1990) as well as U.S. Pat. Nos. 5,580,859; 5,589,466; 5,804,566; 5,739,118; 5,736,524; 5,679,647; WO 98/04720. Examples of DNA-based delivery technologies include “naked DNA”, facilitated (bupivicaine, polymers, peptide-mediated) delivery, cationic lipid complexes, and particle-mediated (“gene gun”) or pressure-mediated delivery (see, e.g., U.S. Pat. No. 5,922,687).

[0295] For therapeutic or prophylactic immunization purposes, proteins of the invention can be expressed via viral or bacterial vectors. Various viral gene delivery systems that can be used in the practice of the invention include, but are not limited to, vaccinia, fowlpox, canarypox, adenovirus, influenza, poliovirus, adeno-associated virus, lentivirus, and sindbis virus (see, e.g., Restifo, 1996, Curr. Opin. Immunol. 8:658-663; Tsang et al. J. Natl. Cancer Inst. 87:982-990 (1995)). Non-viral delivery systems can also be employed by introducing naked DNA encoding a 121P1F1-related protein into the patient (e.g., intramuscularly or intradermally) to induce an anti-tumor response.

[0296] Vaccinia virus is used, for example, as a vector to express nucleotide sequences that encode the peptides of the invention. Upon introduction into a host, the recombinant vaccinia virus expresses the protein immunogenic peptide, and thereby elicits a host immune response. Vaccinia vectors and methods useful in immunization protocols are described in, e.g., U.S. Pat. No. 4,722,848. Another vector is BCG (Bacille Calmette Guerin). BCG vectors are described in Stover et al., Nature 351:456-460 (1991). A wide variety of other vectors useful for therapeutic administration or immunization of the peptides of the invention, e.g. adeno and adeno-associated virus vectors, retroviral vectors, Salmonella typhi vectors, detoxified anthrax toxin vectors, and the like, will be apparent to those skilled in the art from the description herein.

[0297] Thus, gene delivery systems are used to deliver a 121P1F1-related nucleic acid molecule. In one embodiment, the full-length human 121P1F1 cDNA is employed. In another embodiment, 121P1F1 nucleic acid molecules encoding specific cytotoxic T lymphocyte (CTL) and/or antibody epitopes are employed.

[0298] Ex Vivo Vaccines

[0299] Various ex vivo strategies can also be employed to generate an immune response. One approach involves the use of antigen presenting cells (APCs) such as dendritic cells (DC) to present 121P1F1 antigen to a patient's immune system. Dendritic cells express MHC class I and II molecules, B7 co-stimulator, and IL-12, and are thus highly specialized antigen presenting cells. In prostate cancer, autologous dendritic cells pulsed with peptides of the prostate-specific membrane antigen (PSMA) are being used in a Phase I clinical trial to stimulate prostate cancer patients' immune systems (Tjoa et al., 1996, Prostate 28:65-69; Murphy et al., 1996, Prostate 29:371-380). Thus, dendritic cells can be used to present 121P1F1 peptides to T cells in the context of MHC class I or II molecules. In one embodiment, autologous dendritic cells are pulsed with 121P1F1 peptides capable of binding to MHC class I and/or class II molecules. In another embodiment, dendritic cells are pulsed with the complete 121P1F1 protein. Yet another embodiment involves engineering the overexpression of a 121P1F1 gene in dendritic cells using various implementing vectors known in the art, such as adenovirus (Arthur et al., 1997, Cancer Gene Ther. 4:17-25), retrovirus (Henderson et al., 1996, Cancer Res. 56:3763-3770), lentivirus, adeno-associated virus, DNA transfection (Ribas et al., 1997, Cancer Res. 57:2865-2869), or tumor-derived RNA transfection (Ashley et al., 1997, J. Exp. Med. 186:1177-1182). Cells that express 121P1F1 can also be engineered to express immune modulators, such as GM-CSF, and used as immunizing agents.

[0300] X.B.) 121P1F1 as a Target for Antibody-Based Therapy

[0301] 121P1F1 is an attractive target for antibody-based therapeutic strategies. A number of antibody strategies are known in the art for targeting both extracellular and intracellular molecules (see, e.g., complement and ADCC mediated killing as well as the use of intrabodies). Because 121P1F1 is expressed by cancer cells of various lineages relative to corresponding normal cells, systemic administration of 121P1F1-immunoreactive compositions are prepared that exhibit excellent sensitivity without toxic, non-specific and/or non-target effects caused by binding of the immunoreactive composition to non-target organs and tissues. Antibodies specifically reactive with domains of 121P1F1 are useful to treat 121P1F1-expressing cancers systemically, either as conjugates with a toxin or therapeutic agent, or as naked antibodies capable of inhibiting cell proliferation or function.

[0302] 121P1F1 antibodies can be introduced into a patient such that the antibody binds to 121P1F1 and modulates a function, such as an interaction with a binding partner, and consequently mediates destruction of the tumor cells and/or inhibits the growth of the tumor cells. Mechanisms by which such antibodies exert a therapeutic effect can include complement-mediated cytolysis, antibody-dependent cellular cytotoxicity, modulation of the physiological function of 121P1F1, inhibition of ligand binding or signal transduction pathways, modulation of tumor cell differentiation, alteration of tumor angiogenesis factor profiles, and/or apoptosis.

[0303] Those skilled in the art understand that antibodies can be used to specifically target and bind immunogenic molecules such as an immunogenic region of a 121P1F1 sequence shown in FIG. 2 or FIG. 3. In addition, skilled artisans understand that it is routine to conjugate antibodies to cytotoxic agents (see e.g., Slevers et al. Blood 93:11 3678-3684 (Jun. 1, 1999)). When cytotoxic and/or therapeutic agents are delivered directly to cells, such as by conjugating them to antibodies specific for a molecule expressed by that cell (e.g. 121P1F1), the cytotoxic agent will exert its known biological effect (i.e. cytotoxicity) on those cells.

[0304] A wide variety of compositions and methods for using antibody-cytotoxic agent conjugates to kill cells are known in the art. In the context of cancers, typical methods entail administering to an animal having a tumor a biologically effective amount of a conjugate comprising a selected cytotoxic and/or therapeutic agent linked to a targeting agent (e.g. an anti-121P1F1 antibody) that binds to a marker (e.g. 121P1F1) expressed, accessible to binding or localized on the cell surfaces. A typical embodiment is a method of delivering a cytotoxic and/or therapeutic agent to a cell expressing 121P1F1, comprising conjugating the cytotoxic agent to an antibody that immunospecifically binds to a 121P1F1 epitope, and, exposing the cell to the antibody-agent conjugate. Another illustrative embodiment is a method of treating an individual suspected of suffering from metastasized cancer, comprising a step of administering parenterally to said individual a pharmaceutical composition comprising a therapeutically effective amount of an antibody conjugated to a cytotoxic and/or therapeutic agent.

[0305] Cancer immunotherapy using anti-121P1F1 antibodies can be done in accordance with various approaches that have been successfully employed in the treatment of other types of cancer, including but not limited to colon cancer (Arlen et al., 1998, Crit. Rev. Immunol. 18:133-138), multiple myeloma (Ozaki et al., 1997, Blood 90:3179-3186, Tsunenari et al., 1997, Blood 90:2437-2444), gastric cancer (Kasprzyk et al., 1992, Cancer Res. 52:2771-2776), B-cell lymphoma (Funakoshi et al., 1996, J. Immunother. Emphasis Tumor Immunol. 19:93-101), leukemia (Zhong et al., 1996, Leuk. Res. 20:581-589), colorectal cancer (Moun et al., 1994, Cancer Res. 54:6160-6166; Velders et al., 1995, Cancer Res. 55:4398-4403), and breast cancer (Shepard et al., 1991, J. Clin. Immunol. 11:117-127). Some therapeutic approaches involve conjugation of naked antibody to a toxin or radioisotope, such as the conjugation of Y⁹¹ or I¹³¹ to anti-CD20 antibodies (e.g., Zevalin™, IDEC Pharmaceuticals Corp. or Bexxar™, Coulter Pharmaceuticals), while others involve co-administration of antibodies and other therapeutic agents, such as Herceptin™ (trastuzumab) with paclitaxel (Genentech, Inc.). The antibodies can be conjugated to a therapeutic agent. To treat prostate cancer, for example, 121P1F1 antibodies can be administered in conjunction with radiation, chemotherapy or hormone ablation. Also, antibodies can be conjugated to a toxin such as calicheamicin (e.g., Mylotarg™, Wyeth-Ayerst, Madison, N.J., a recombinant humanized IgG₄ kappa antibody conjugated to antitumor antibiotic calicheamicin) or a maytansinoid (e.g., taxane-based Tumor-Activated Prodrug, TAP, platform, ImmunoGen, Cambridge, Mass., also see e.g., U.S. Pat. No. 5,416,064).

[0306] Although 121P1F1 antibody therapy is useful for all stages of cancer, antibody therapy can be particularly appropriate in advanced or metastatic cancers. Treatment with the antibody therapy of the invention is indicated for patients who have received one or more rounds of chemotherapy. Alternatively, antibody therapy of the invention is combined with a chemotherapeutic or radiation regimen for patients who have not received chemotherapeutic treatment. Additionally, antibody therapy can enable the use of reduced dosages of concomitant chemotherapy, particularly for patients who do not tolerate the toxicity of the chemotherapeutic agent very well. Fan et al. (Cancer Res. 53:4637-4642, 1993), Prewett et al. (International J. of Onco. 9:217-224, 1996), and Hancock et al. (Cancer Res. 51:4575-4580, 1991) describe the use of various antibodies together with chemotherapeutic agents.

[0307] Although 121P1F1 antibody therapy is useful for all stages of cancer, antibody therapy can be particularly appropriate in advanced or metastatic cancers. Treatment with the antibody therapy of the invention is indicated for patients who have received one or more rounds of chemotherapy. Alternatively, antibody therapy of the invention is combined with a chemotherapeutic or radiation regimen for patients who have not received chemotherapeutic treatment. Additionally, antibody therapy can enable the use of reduced dosages of concomitant chemotherapy, particularly for patients who do not tolerate the toxicity of the chemotherapeutic agent very well.

[0308] Cancer patients can be evaluated for the presence and level of 121P1F1 expression, preferably using immunohistochemical assessments of tumor tissue, quantitative 121P1F1 imaging, or other techniques that reliably indicate the presence and degree of 121P1F1 expression. Immunohistochemical analysis of tumor biopsies or surgical specimens is preferred for this purpose. Methods for immunohistochemical analysis of tumor tissues are well known in the art.

[0309] Anti-121P1F1 monoclonal antibodies that treat prostate and other cancers include those that initiate a potent immune response against the tumor or those that are directly cytotoxic. In this regard, anti-121P1F1 monoclonal antibodies (mAbs) can elicit tumor cell lysis by either complement-mediated or antibody-dependent cell cytotoxicity (ADCC) mechanisms, both of which require an intact Fc portion of the immunoglobulin molecule for interaction with effector cell Fc receptor sites on complement proteins. In addition, anti-121P1F1 mAbs that exert a direct biological effect on tumor growth are useful to treat cancers that express 121P1F1. Mechanisms by which directly cytotoxic mAbs act include: inhibition of cell growth, modulation of cellular differentiation, modulation of tumor angiogenesis factor profiles, and the induction of apoptosis. The mechanism(s) by which a particular anti-121P1F1 mAb exerts an anti-tumor effect is evaluated using any number of in vitro assays that evaluate cell death such as ADCC, ADMMC, complement-mediated cell lysis, and so forth, as is generally known in the art.

[0310] In some patients, the use of murine or other non-human monoclonal antibodies, or human/mouse chimeric mAbs can induce moderate to strong immune responses against the non-human antibody. This can result in clearance of the antibody from circulation and reduced efficacy. In the most severe cases, such an immune response can lead to the extensive formation of immune complexes which, potentially, can cause renal failure. Accordingly, preferred monoclonal antibodies used in the therapeutic methods of the invention are those that are either fully human or humanized and that bind specifically to the target 121P1F1 antigen with high affinity but exhibit low or no antigenicity in the patient.

[0311] Therapeutic methods of the invention contemplate the administration of single anti-121P1F1 mAbs as well as combinations, or cocktails, of different mAbs. Such mAb cocktails can have certain advantages inasmuch as they contain mAbs that target different epitopes, exploit different effector mechanisms or combine directly cytotoxic mAbs with mAbs that rely on immune effector functionality. Such mAbs in combination can exhibit synergistic therapeutic effects. In addition, anti-121P1F1 mAbs can be administered concomitantly with other therapeutic modalities, including but not limited to various chemotherapeutic agents, androgen-blockers, immune modulators (e.g., IL-2, GM-CSF), surgery or radiation. The anti-121P1F1 mAbs are administered in their “naked” or unconjugated form, or can have a therapeutic agent(s) conjugated to them.

[0312] Anti-121PI F1 antibody formulations are administered via any route capable of delivering the antibodies to a tumor cell. Routes of administration include, but are not limited to, intravenous, intraperitoneal, intramuscular, intratumor, intradermal, and the like. Treatment generally involves repeated administration of the anti-121P1F1 antibody preparation, via an acceptable route of administration such as intravenous injection (IV), typically at a dose in the range of about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, or 25 mg/kg body weight. In general, doses in the range of 10-1000 mg mAb per week are effective and well tolerated.

[0313] Based on clinical experience with the Herceptin™ mAb in the treatment of metastatic breast cancer, an initial loading dose of approximately 4 mg/kg patient body weight IV, followed by weekly doses of about 2 mg/kg IV of the anti-121P1F1 mAb preparation represents an acceptable dosing regimen. Preferably, the initial loading dose is administered as a 90 minute or longer infusion. The periodic maintenance dose is administered as a 30 minute or longer infusion, provided the initial dose was well tolerated. As appreciated by those of skill in the art, various factors can influence the ideal dose regimen in a particular case. Such factors include, for example, the binding affinity and half life of the Ab or mAbs used, the degree of 121P1F1 expression in the patient, the extent of circulating shed 121P1F1 antigen, the desired steady-state antibody concentration level, frequency of treatment, and the influence of chemotherapeutic or other agents used in combination with the treatment method of the invention, as well as the health status of a particular patient.

[0314] Optionally, patients should be evaluated for the levels of 121P1F1 in a given sample (e.g. the levels of circulating 121P1F1 antigen and/or 121P1F1 expressing cells) in order to assist in the determination of the most effective dosing regimen, etc. Such evaluations are also used for monitoring purposes throughout therapy, and are useful to gauge therapeutic success in combination with the evaluation of other parameters (for example, urine cytology and/or ImmunoCyt levels in bladder cancer therapy, or by analogy, serum PSA levels in prostate cancer therapy).

[0315] Anti-idiotypic anti-121P1F1 antibodies can also be used in anti-cancer therapy as a vaccine for inducing an immune response to cells expressing a 121P1F1-related protein. In particular, the generation of anti-idiotypic antibodies is well known in the art; this methodology can readily be adapted to generate anti-idiotypic anti-121P1F1 antibodies that mimic an epitope on a 121P1F1-related protein (see, for example, Wagner et al., 1997, Hybridoma 16: 33-40; Foon et al., 1995, J. Clin. Invest. 96:334-342; Herlyn et al., 1996, Cancer Immunol. Immunother. 43:65-76). Such an anti-idiotypic antibody can be used in cancer vaccine strategies.

[0316] X.C.) 121P1F1 as a Target for Cellular Immune Responses

[0317] Vaccines and methods of preparing vaccines that contain an immunogenically effective amount of one or more HLA-binding peptides as described herein are further embodiments of the invention. Furthermore, vaccines in accordance with the invention encompass compositions of one or more of the claimed peptides. A peptide can be present in a vaccine individually. Alternatively, the peptide can exist as a homopolymer comprising multiple copies of the same peptide, or as a heteropolymer of various peptides. Polymers have the advantage of increased immunological reaction and, where different peptide epitopes are used to make up the polymer, the additional ability to induce antibodies and/or CTLs that react with different antigenic determinants of the pathogenic organism or tumor-related peptide targeted for an immune response. The composition can be a naturally occurring region of an antigen or can be prepared, e.g., recombinantly or by chemical synthesis.

[0318] Carriers that can be used with vaccines of the invention are well known in the art, and include, e.g., thyroglobulin, albumins such as human serum albumin, tetanus toxoid, polyamino acids such as poly L-lysine, poly L-glutamic acid, influenza, hepatitis B virus core protein, and the like. The vaccines can contain a physiologically tolerable (i.e., acceptable) diluent such as water, or saline, preferably phosphate buffered saline. The vaccines also typically include an adjuvant. Adjuvants such as incomplete Freund's adjuvant, aluminum phosphate, aluminum hydroxide, or alum are examples of materials well known in the art. Additionally, as disclosed herein, CTL responses can be primed by conjugating peptides of the invention to lipids, such as tripalmitoyl-S-glycerylcysteinlyseryl-serine (P₃CSS). Moreover, an adjuvant such as a synthetic cytosine-phosphorothiolated-guanine-containing (CpG) oligonucleotides has been found to increase CTL responses 10- to 100-fold. (see, e.g. Davila and Celis J. Immunol. 165:539-547 (2000))

[0319] Upon immunization with a peptide composition in accordance with the invention, via injection, aerosol, oral, transdermal, transmucosal, intrapleural, intrathecal, or other suitable routes, the immune system of the host responds to the vaccine by producing large amounts of CTLs and/or HTLs specific for the desired antigen. Consequently, the host becomes at least partially immune to later development of cells that express or overexpress 121P1F1 antigen, or derives at least some therapeutic benefit when the antigen was tumor-associated.

[0320] In some embodiments, it may be desirable to combine the class I peptide components with components that induce or facilitate neutralizing antibody and or helper T cell responses directed to the target antigen. A preferred embodiment of such a composition comprises class I and class II epitopes in accordance with the invention. An alternative embodiment of such a composition comprises a class I and/or class II epitope in accordance with the invention, along with a cross reactive HTL epitope such as PADRE™ (Epimmune, San Diego, Calif.) molecule (described e.g., in U.S. Pat. No. 5,736,142).

[0321] A vaccine of the invention can also include antigen-presenting cells (APC), such as dendritic cells (DC), as a vehicle to present peptides of the invention. Vaccine compositions can be created in vitro, following dendritic cell mobilization and harvesting, whereby loading of dendritic cells occurs in vitro. For example, dendritic cells are transfected, e.g., with a minigene in accordance with the invention, or are pulsed with peptides. The dendritic cell can then be administered to a patient to elicit immune responses in vivo. Vaccine compositions, either DNA- or peptide-based, can also be administered in vivo in combination with dendritic cell mobilization whereby loading of dendritic cells occurs in vivo.

[0322] Preferably, the following principles are utilized when selecting an array of epitopes for inclusion in a polyepitopic composition for use in a vaccine, or for selecting discrete epitopes to be included in a vaccine and/or to be encoded by nucleic acids such as a minigene. It is preferred that each of the following principles be balanced in order to make the selection. The multiple epitopes to be incorporated in a given vaccine composition may be, but need not be, contiguous in sequence in the native antigen from which the epitopes are derived.

[0323] 1.) Epitopes are selected which, upon administration, mimic immune responses that have been observed to be correlated with tumor clearance. For HLA Class I this includes 3-4 epitopes that come from at least one tumor associated antigen (TAA). For HLA Class II a similar rationale is employed; again 3-4 epitope, are selected from at least one TAA (see, e.g., Rosenberg et al., Science 278:1447-1450). Epitopes from one TAA may be used in combination with epitopes from one or more additional TAAs to produce a vaccine that targets tumors with varying expression patterns of frequently-expressed TAAs.

[0324] 2.) Epitopes are selected that have the requisite binding affinity established to be correlated with immunogenicity: for HLA Class I an IC₅₀ of 500 nM or less, often 200 nM or less; and for Class II an IC₅₀ of 1000 nM or less.

[0325] 3.) Sufficient supermotif bearing-peptides, or a sufficient array of allele-specific motif-bearing peptides, are selected to give broad population coverage. For example, it is preferable to have at least 80% population coverage. A Monte Carlo analysis, a statistical evaluation known in the art, can be employed to assess the breadth, or redundancy of, population coverage.

[0326] 4.) When selecting epitopes from cancer-related antigens it is often useful to select analogs because the patient may have developed tolerance to the native epitope.

[0327] 5.) Of particular relevance are epitopes referred to as “nested epitopes.” Nested epitopes occur where at least two epitopes overlap in a given peptide sequence. A nested peptide sequence can comprise B cell, HLA class I and/or HLA class II epitopes. When providing nested epitopes, a general objective is to provide the greatest number of epitopes per sequence. Thus, an aspect is to avoid providing a peptide that is any longer than the amino terminus of the amino terminal epitope and the carboxyl terminus of the carboxyl terminal epitope in the peptide. When providing a multi-epitopic sequence, such as a sequence comprising nested epitopes, it is generally important to screen the sequence in order to insure that it does not have pathological or other deleterious biological properties.

[0328] 6.) If a polyepitopic protein is created, or when creating a minigene, an objective is to generate the smallest peptide that encompasses the epitopes of interest. This principle is similar, if not the same as that employed when selecting a peptide comprising nested epitopes. However, with an artificial polyepitopic peptide, the size minimization objective is balanced against the need to integrate any spacer sequences between epitopes in the polyepitopic protein. Spacer amino acid residues can, for example, be introduced to avoid junctional epitopes (an epitope recognized by the immune system, not present in the target antigen, and only created by the man-made juxtaposition of epitopes), or to facilitate cleavage between epitopes and thereby enhance epitope presentation. Junctional epitopes are generally to be avoided because the recipient may generate an immune response to that non-native epitope. Of particular concern is a junctional epitope that is a “dominant epitope.” A dominant epitope may lead to such a zealous response that immune responses to other epitopes are diminished or suppressed.

[0329] 7.) Where the sequences of multiple variants of the same target protein are present, potential peptide epitopes can also be selected on the basis of their conservancy. For example, a criterion for conservancy may define that the entire sequence of an HLA class I binding peptide or the entire 9-mer core of a class II binding peptide be conserved in a designated percentage of the sequences evaluated for a specific protein antigen.

[0330] X.C.1. Minigene Vaccines

[0331] A number of different approaches are available which allow simultaneous delivery of multiple epitopes. Nucleic acids encoding the peptides of the invention are a particularly useful embodiment of the invention. Epitopes for inclusion in a minigene are preferably selected according to the guidelines set forth in the previous section. A preferred means of administering nucleic acids encoding the peptides of the invention uses minigene constructs encoding a peptide comprising one or multiple epitopes of the invention.

[0332] The use of multi-epitope minigenes is described below and in, Ishioka et al., J. Immunol. 162:3915-3925, 1999; An, L. and Whitton, J. L., J. Virol. 71:2292, 1997; Thomson, S. A. et al., J. Immunol. 1.57:822, 1996; Whitton, J. L. et al., J. Virol. 67:348, 1993; Hanke, R. et al., Vaccine 16:426, 1998. For example, a multi-epitope DNA plasmid encoding supermotif- and/or motif-bearing epitopes derived 121P1F1, the PADRE® universal helper T cell epitope (or multiple HTL epitopes from 121P1F1), and an endoplasmic reticulum-translocating signal sequence can be engineered. A vaccine may also comprise epitopes that are derived from other TAAs.

[0333] The immunogenicity of a multi-epitopic minigene can be confirmed in transgenic mice to evaluate the magnitude of CTL induction responses against the epitopes tested. Further, the immunogenicity of DNA-encoded epitopes in vivo can be correlated with the in vitro responses of specific CTL lines against target cells transfected with the DNA plasmid. Thus, these experiments can show that the minigene serves to both: 1.) generate a CTL response and 2.) that the induced CTLs recognized cells expressing the encoded epitopes.

[0334] For example, to create a DNA sequence encoding the selected epitopes (minigene) for expression in human cells, the amino acid sequences of the epitopes may be reverse translated. A human codon usage table can be used to guide the codon choice for each amino acid. These epitope-encoding DNA sequences may be directly adjoined, so that when translated, a continuous polypeptide sequence is created. To optimize expression and/or immunogenicity, additional elements can be incorporated into the minigene design. Examples of amino acid sequences that can be reverse translated and included in the minigene sequence include: HLA class I epitopes, HLA class II epitopes, antibody epitopes, a ubiquitination signal sequence, and/or an endoplasmic reticulum targeting signal. In addition, HLA presentation of CTL and HTL epitopes may be improved by including synthetic (e.g. poly-alanine) or naturally-occurring flanking sequences adjacent to the CTL or HTL epitopes; these larger peptides comprising the epitope(s) are within the scope of the invention.

[0335] The minigene sequence may be converted to DNA by assembling oligonucleotides that encode the plus and minus strands of the minigene. Overlapping oligonucleotides (30-100 bases long) may be synthesized, phosphorylated, purified and annealed under appropriate conditions using well known techniques. The ends of the oligonucleotides can be joined, for example, using T4 DNA ligase. This synthetic minigene, encoding the epitope polypeptide, can then be cloned into a desired expression vector.

[0336] Standard regulatory sequences well known to those of skill in the art are preferably included in the vector to ensure expression in the target cells. Several vector elements are desirable: a promoter with a down-stream cloning site for minigene insertion; a polyadenylation signal for efficient transcription termination; an E. coli origin of replication; and an E. coli selectable marker (e.g. ampicillin or kanamycin resistance). Numerous promoters can be used for this purpose, e.g., the human cytomegalovirus (hCMV) promoter. See, e.g., U.S. Pat. Nos. 5,580,859 and 5,589,466 for other suitable promoter sequences.

[0337] Additional vector modifications may be desired to optimize minigene expression and immunogenicity. In some cases, introns are required for efficient gene expression, and one or more synthetic or naturally-occurring introns could be incorporated into the transcribed region of the minigene. The inclusion of mRNA stabilization sequences and sequences for replication in mammalian cells may also be considered for increasing minigene expression.

[0338] Once an expression vector is selected, the minigene is cloned into the polylinker region downstream of the promoter. This plasmid is transformed into an appropriate E. coli strain, and DNA is prepared using standard techniques. The orientation and DNA sequence of the minigene, as well as all other elements included in the vector, are confirmed using restriction mapping and DNA sequence analysis. Bacterial cells harboring the correct plasmid can be stored as a master cell bank and a working cell bank.

[0339] In addition, immunostimulatory sequences (ISSs or CpGs) appear to play a role in the immunogenicity of DNA vaccines. These sequences may be included in the vector, outside the minigene coding sequence, if desired to enhance immunogenicity.

[0340] In some embodiments, a bi-cistronic expression vector which allows production of both the minigene-encoded epitopes and a second protein (included to enhance or decrease immunogenicity) can be used. Examples of proteins or polypeptides that could beneficially enhance the immune response if co-expressed include cytokines (e.g., IL-2, IL-12, GM-CSF), cytokine-inducing molecules (e.g., LeIF), costimulatory molecules, or for HTL responses, pan-DR binding proteins (PADRE™, Epimmune, San Diego, Calif.). Helper (HTL) epitopes can be joined to intracellular targeting signals and expressed separately from expressed CTL epitopes; this allows direction of the HTL epitopes to a cell compartment different than that of the CTL epitopes. If required, this could facilitate more efficient entry of HTL epitopes into the HLA class II pathway, thereby improving HTL induction. In contrast to HTL or CTL induction, specifically decreasing the immune response by co-expression of immunosuppressive molecules (e.g. TGF-β) may be beneficial in certain diseases.

[0341] Therapeutic quantities of plasmid DNA can be produced for example, by fermentation in E. coli, followed by purification. Aliquots from the working cell bank are used to inoculate growth medium, and grown to saturation in shaker flasks or a bioreactor according to well-known techniques. Plasmid DNA can be purified using standard bioseparation technologies such as solid phase anion-exchange resins supplied by QIAGEN, Inc. (Valencia, Calif.). If required, supercoiled DNA can be isolated from the open circular and linear forms using gel electrophoresis or other methods.

[0342] Purified plasmid DNA can be prepared for injection using a variety of formulations. The simplest of these is reconstitution of lyophilized DNA in sterile phosphate-buffer saline (PBS). This approach, known as “naked DNA,” is currently being used for intramuscular (IM) administration in clinical trials. To maximize the immunotherapeutic effects of minigene DNA vaccines, an alternative method for formulating purified plasmid DNA may be desirable. A variety of methods have been described, and new techniques may become available. Cationic lipids, glycolipids, and fusogenic liposomes can also be used in the formulation (see, e.g., as described by WO 93/24640; Mamino & Gould-Fogerite, BioTechniques 6(7): 682 (1988); U.S. Pat. No. 5,279,833; WO 91/06309; and Felgner, et al., Proc. Nat'l Acad. Sci. USA 84:7413 (1987). In addition, peptides and compounds referred to collectively as protective, interactive, non-condensing compounds (PINC) could also be complexed to purified plasmid DNA to influence variables such as stability, intramuscular dispersion, or trafficking to specific organs or cell types.

[0343] Target cell sensitization can be used as a functional assay for expression and HLA class I presentation of minigene-encoded CTL epitopes. For example, the plasmid DNA is introduced into a mammalian cell line that is suitable as a target for standard CTL chromium release assays. The transfection method used will be dependent on the final formulation. Electroporation can be used for “naked” DNA, whereas cationic lipids allow direct in vitro transfection. A plasmid expressing green fluorescent protein (GFP) can be co-transfected to allow enrichment of transfected cells using fluorescence activated cell sorting (FACS). These cells are then chromium-51 (⁵¹Cr) labeled and used as target cells for epitope-specific CTL lines; cytolysis, detected by ⁵¹Cr release, indicates both production of, and HLA presentation of, minigene-encoded CTL epitopes. Expression of HTL epitopes may be evaluated in an analogous manner using assays to assess HTL activity.

[0344] In vivo immunogenicity is a second approach for functional testing of minigene DNA formulations. Transgenic mice expressing appropriate human HLA proteins are immunized with the DNA product. The dose and route of administration are formulation dependent (e.g., IM for DNA in PBS, intraperitoneal (i.p.) for lipid-complexed DNA). Twenty-one days after immunization, splenocytes are harvested and restimulated for one week in the presence of peptides encoding each epitope being tested. Thereafter, for CTL effector cells, assays are conducted for cytolysis of peptide-loaded, ⁵¹Cr-labeled target cells using standard techniques. Lysis of target cells that were sensitized by HLA loaded with peptide epitopes, corresponding to minigene-encoded epitopes, demonstrates DNA vaccine function for in vivo induction of CTLs. Immunogenicity of HTL epitopes is confirmed in transgenic mice in an analogous manner.

[0345] Alternatively, the nucleic acids can be administered using ballistic delivery as described, for instance, in U.S. Pat. No. 5,204,253. Using this technique, particles comprised solely of DNA are administered. In a further alternative embodiment, DNA can be adhered to particles, such as gold particles.

[0346] Minigenes can also be delivered using other bacterial or viral delivery systems well known in the art, e.g., an expression construct encoding epitopes of the invention can be incorporated into a viral vector such as vaccinia.

[0347] X.C.2. Combinations of CTL Peptides with Helper Peptides

[0348] Vaccine compositions comprising CTL peptides of the invention can be modified, e.g., analoged, to provide desired attributes, such as improved serum half life, broadened population coverage or enhanced immunogenicity.

[0349] For instance, the ability of a peptide to induce CTL activity can be enhanced by linking the peptide to a sequence which contains at least one epitope that is capable of inducing a T helper cell response. Although a CTL peptide can be directly linked to a T helper peptide, often CTL epitope/HTL epitope conjugates are linked by a spacer molecule. The spacer is typically comprised of relatively small, neutral molecules, such as amino acids or amino acid mimetics, which are substantially uncharged under physiological conditions. The spacers are typically selected from, e.g., Ala, Gly, or other neutral spacers of nonpolar amino acids or neutral polar amino acids. It will be understood that the optionally present spacer need not be comprised of the same residues and thus may be a hetero- or homo-oligomer. When present, the spacer will usually be at least one or two residues, more usually three to six residues and sometimes 10 or more residues. The CTL peptide epitope can be linked to the T helper peptide epitope either directly or via a spacer either at the amino or carboxy terminus of the CTL peptide. The amino terminus of either the immunogenic peptide or the T helper peptide may be acylated.

[0350] In certain embodiments, the T helper peptide is one that is recognized by T helper cells present in a majority of a genetically diverse population. This can be accomplished by selecting peptides that bind to many, most, or all of the HLA class II molecules. Examples of such amino acid bind many HLA Class II molecules include sequences from antigens such as tetanus toxoid at positions 830-843 (QYIKANSKFIGITE; SEQ ID NO: ______), Plasmodium falciparum circumsporozoite (CS) protein at positions 378-398 (DIEKKIAKMEKASSVFNVVNS; SEQ ID NO: ______), and Streptococcus 18 kD protein at positions 116-131 (GAVDSILGGVATYGAA; SEQ ID NO: ______). Other examples include peptides bearing a DR 1-4-7 supermotif, or either of the DR3 motifs.

[0351] Alternatively, it is possible to prepare synthetic peptides capable of stimulating T helper lymphocytes, in a loosely HLA-restricted fashion, using amino acid sequences not found in nature (see, e.g., PCT publication WO 95/07707). These synthetic compounds called Pan-DR-binding epitopes (e.g., PADRE™, Epimmune, Inc., San Diego, Calif.) are designed to most preferably bind most HLA-DR (human HLA class II) molecules. For instance, a pan-DR-binding epitope peptide having the formula: aKXVAAVJTLKAAa (SEQ ID NO: ______), where “X” is either cyclohexylalanine, phenylalanine, or tyrosine, and a is either D-alanine or L-alanine, has been found to bind to most HLA-DR alleles, and to stimulate the response of T helper lymphocytes from most individuals, regardless of their HLA type. An alternative of a pan-DR binding epitope comprises all “L” natural amino acids and can be provided in the form of nucleic acids that encode the epitope.

[0352] HTL peptide epitopes can also be modified to alter their biological properties. For example, they can be modified to include D-amino acids to increase their resistance to proteases and thus extend their serum half life, or they can be conjugated to other molecules such as lipids, proteins, carbohydrates, and the like to increase their biological activity. For example, a T helper peptide can be conjugated to one or more palmitic acid chains at either the amino or carboxyl termini.

[0353] X.C.3. Combinations of CTL Peptides with T Cell Priming Agents

[0354] In some embodiments it may be desirable to include in the pharmaceutical compositions of the invention at least one component which primes B lymphocytes or T lymphocytes. Lipids have been identified as agents capable of priming CTL in vivo. For example, palmitic acid residues can be attached to the ε- and α-amino groups of a lysine residue and then linked, e.g., via one or more linking residues such as Gly, Gly-Gly-, Ser, Ser-Ser, or the like, to an immunogenic peptide. The lipidated peptide can then be administered either directly in a micelle or particle, incorporated into a liposome, or emulsified in an adjuvant, e.g., incomplete Freund's adjuvant. In a preferred embodiment, a particularly effective immunogenic composition comprises palmitic acid attached to ε- and α-amino groups of Lys, which is attached via linkage, e.g., Ser-Ser, to the amino terminus of the immunogenic peptide.

[0355] As another example of lipid priming of CTL responses, E. coli lipoproteins, such as tripalmitoyl-S-glycerylc:ysteinlyseryl-serine (P₃CSS) can be used to prime virus specific CTL when covalently attached to an appropriate peptide (see, e.g., Deres, et al., Nature 342:561, 1989). Peptides of the invention can be coupled to P₃CSS, for example, and the lipopeptide administered to an individual to specifically prime an immune response to the target antigen. Moreover, because the induction of neutralizing antibodies can also be primed with P₃CSS-conjugated epitopes, two such compositions can be combined to more effectively elicit both humoral and cell-mediated responses.

[0356] X.C.4. Vaccine Compositions Comprising DC Pulsed with CTL and/or HTL Peptides

[0357] An embodiment of a vaccine composition in accordance with the invention comprises ex vivo administration of a cocktail of epitope-bearing peptides to PBMC, or isolated DC therefrom, from the patient's blood. A pharmaceutical to facilitate harvesting of DC can be used, such as Progenipoietin™ (Pharmacia-Monsanto, St. Louis, Mo.) or GM-CSF/IL-4. After pulsing the DC with peptides and prior to reinfusion into patients, the DC are washed to remove unbound peptides. In this embodiment, a vaccine comprises peptide-pulsed DCs which present the pulsed peptide epitopes complexed with HLA molecules on their surfaces.

[0358] The DC can be pulsed ex vivo with a cocktail of peptides, some of which stimulate CTL responses to 121P1F1. Optionally, a helper T cell (HTL) peptide, such as a natural or artificial loosely restricted HLA Class II peptide, can be included to facilitate the CTL response. Thus, a vaccine in accordance with the invention is used to treat a cancer which expresses or overexpresses 121P1Fl.

[0359] X.D. Adoptive Immunotherapy

[0360] Antigenic 121P1F1-related peptides are used to elicit a CTL and/or HTL response ex vivo, as well. The resulting CTL or HTL cells, can be used to treat tumors in patients that do not respond to other conventional forms of therapy, or will not respond to a therapeutic vaccine peptide or nucleic acid in accordance with the invention. Ex vivo CTL or HTL responses to a particular antigen are induced by incubating in tissue culture the patient's, or genetically compatible, CTL or HTL precursor cells together with a source of antigen-presenting cells (APC), such as dendritic cells, and the appropriate immunogenic peptide. After an appropriate incubation time (typically about 7-28 days), in which the precursor cells are activated and expanded into effector cells, the cells are infused back into the patient, where they will destroy (CTL) or facilitate destruction (HTL) of their specific target cell (e.g., a tumor cell). Transfected dendritic cells may also be used as antigen presenting cells.

[0361] X.E. Administration of Vaccines for Therapeutic or Prophylactic Purposes

[0362] Pharmaceutical and vaccine compositions of the invention are typically used to treat and/or prevent a cancer that expresses or overexpresses 121P1F1. In therapeutic applications, peptide and/or nucleic acid compositions are administered to a patient in an amount sufficient to elicit an effective B cell, CTL and/or HTL response to the antigen and to cure or at least partially arrest or slow symptoms and/or complications. An amount adequate to accomplish this is defined as “therapeutically effective dose.” Amounts effective for this use will depend on, e.g., the particular composition administered, the manner of administration, the stage and severity of the disease being treated, the weight and general state of health of the patient, and the judgment of the prescribing physician.

[0363] For pharmaceutical compositions, the immunogenic peptides of the invention, or DNA encoding them, are generally administered to an individual already bearing a tumor that expresses 121P1F1. The peptides or DNA encoding them can be administered individually or as fusions of one or more peptide sequences. Patients can be treated with the immunogenic peptides separately or in conjunction with other treatments, such as surgery, as appropriate.

[0364] For therapeutic use, administration should generally begin at the first diagnosis of 121P1F1-associated cancer. This is followed by boosting doses until at least symptoms are substantially abated and for a period thereafter. The embodiment of the vaccine composition (i.e., including, but not limited to embodiments such as peptide cocktails, polyepitopic polypeptides, minigenes, or TAA-specific CTLs or pulsed dendritic cells) delivered to the patient may vary according to the stage of the disease or the patient's health status. For example, in a patient with a tumor that expresses 121P1F1, a vaccine comprising 121P1F1-specific CTL may be more efficacious in killing tumor cells in patient with advanced disease than alternative embodiments.

[0365] It is generally important to provide an amount of the peptide epitope delivered by a mode of administration sufficient to effectively stimulate a cytotoxic T cell response; compositions which stimulate helper T cell responses can also be given in accordance with this embodiment of the invention.

[0366] The dosage for an initial therapeutic immunization generally occurs in a unit dosage range where the lower value is about 1, 5, 50, 500, or 1,000 μg and the higher value is about 10,000; 20,000; 30,000; or 50,000 μg. Dosage values for a human typically range from about 500 μg to about 50,000 μg per 70 kilogram patient. Boosting dosages of between about 1.0 μg to about 50,000 μg of peptide pursuant to a boosting regimen over weeks to months may be administered depending upon the patient's response and condition as determined by measuring the specific activity of CTL and HTL obtained from the patient's blood. Administration should continue until at least clinical symptoms or laboratory tests indicate that the neoplasia, has been eliminated or reduced and for a period thereafter. The dosages, routes of administration, and dose schedules are adjusted in accordance with methodologies known in the art.

[0367] In certain embodiments, the peptides and compositions of the present invention are employed in serious disease states, that is, life-threatening or potentially life threatening situations. In such cases, as a result of the minimal amounts of extraneous substances and the relative nontoxic nature of the peptides in preferred compositions of the invention, it is possible and may be felt desirable by the treating physician to administer substantial excesses of these peptide compositions relative to these stated dosage amounts.

[0368] The vaccine compositions of the invention can also be used purely as prophylactic agents. Generally the dosage for an initial prophylactic immunization generally occurs in a unit dosage range where the lower value is about 1, 5, 50, 500, or 1000 Ag and the higher value is about 10,000; 20,000; 30,000; or 50,000 μg. Dosage values for a human typically range from about 500 μg to about 50,000 μg per 70 kilogram patient. This is followed by boosting dosages of between about 1.0 μg to about 50,000 μg of peptide administered at defined intervals from about four weeks to six months after the initial administration of vaccine. The immunogenicity of the vaccine can be assessed by measuring the specific activity of CTL and HTL obtained from a sample of the patient's blood.

[0369] The pharmaceutical compositions for therapeutic treatment are intended for parenteral, topical, oral, nasal, intrathecal, or local (e.g. as a cream or topical ointment) administration. Preferably, the pharmaceutical compositions are administered parentally, e.g., intravenously, subcutaneously, intradermally, or intramuscularly. Thus, the invention provides compositions for parenteral administration which comprise a solution of the immunogenic peptides dissolved or suspended in an acceptable carrier, preferably an aqueous carrier.

[0370] A variety of aqueous carriers may be used, e.g., water, buffered water, 0.8% saline, 0.3% glycine, hyaluronic acid and the like. These compositions may be sterilized by conventional, well-known sterilization techniques, or may be sterile filtered. The resulting aqueous solutions may be packaged for use as is, or lyophilized, the lyophilized preparation being combined with a sterile solution prior to administration.

[0371] The compositions may contain pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions, such as pH-adjusting and buffering agents, tonicity adjusting agents, wetting agents, preservatives, and the like, for example, sodium acetate, sodium lactate, sodium chloride, potassium chloride, calcium chloride, sorbitan monolaurate, triethanolamine oleate, etc.

[0372] The concentration of peptides of the invention in the pharmaceutical formulations can vary widely, i.e., from less than about 0.1%, usually at or at least about 2% to as much as 20% to 50% or more by weight, and will be selected primarily by fluid volumes, viscosities, etc., in accordance with the particular mode of administration selected.

[0373] A human unit dose form of a composition is typically included in a pharmaceutical composition that comprises a human unit dose of an acceptable carrier, in one embodiment an aqueous carrier, and is administered in a volume/quantity that is known by those of skill in the art to be used for administration of such compositions to humans (see, e.g., Remington's Pharmaceutical Sciences, 17^(th) Edition, A. Gennaro, Editor, Mask Publishing Co., Easton, Pa., 1985). For example a peptide dose for initial immunization can be from about 1 to about 50,000 μg, generally 100-5,000 μg, for a 70 kg patient. For example, for nucleic acids an initial immunization may be performed using an expression vector in the form of naked nucleic acid administered IM (or SC or ID) in the amounts of 0.5-5 mg at multiple sites. The nucleic acid (0.1 to 1000 pg) can also be administered using a gene gun. Following an incubation period of 3-4 weeks, a booster dose is then administered. The booster can be recombinant fowlpox virus administered at a dose of 5−10⁷ to 5×10⁹ pfa.

[0374] For antibodies, a treatment generally involves repeated administration of the anti-121P1F1 antibody preparation, via an acceptable route of administration such as intravenous injection (IV), typically at a dose in the range of about 0.1 to about 10 mg/kg body weight. In general, doses in the range of 10-500 mg mAb par week are effective and well tolerated. Moreover, an initial loading dose of approximately 4 mg/kg patient body weight IV, followed by weekly doses of about 2 mg/kg IV of the anti-121P1F1 mAb preparation represents an acceptable dosing regimen. As appreciated by those of skill in the art, various factors can influence the ideal dose in a particular case. Such factors include, for example, half life of a composition, the binding affinity of an Ab, the immunogenicity of a substance, the degree of 121P1F1 expression in the patient, the extent of circulating shed 121P1F1 antigen, the desired steady-state concentration level, frequency of treatment, and the influence of chemotherapeutic or other agents used in combination with the treatment method of the invention, as well as the health status of a particular patient. Non-limiting preferred human unit doses are, for example, 500 μg-1 mg, 1 mg-50 mg, 50 mg-100 mg, 100 mg-20)mg, 200 mg-300 mg, 400 mg-500 mg, 500 mg-600 mg, 600 mg-700 mg, 700 mg-800 mg, 800 mg-900 mg, 900 mg-1g, or 1 mg-700 mg. In certain embodiments, the dose is in a range of 2-5 mg/kg body weight, e.g., with follow on weekly doses of 1-3 mg/kg; 0.5 mg, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 mg/kg body weight followed, e.g., in two, three or four weeks by weekly doses; 0.5-10 mg/kg body weight, e.g., followed in two, three or four weeks by weekly doses; 225, 250, 275, 300, 325, 350, 375, 400 mg m² of body area weekly; 1-600 mg m² of body area weekly; 225-400 mg m² of body area weekly; these does can be followed by weekly doses for 2, 3, 4, 5, 6, 7, 8, 9, 19, 11, 12 or more weeks.

[0375] In one embodiment, human unit dose forms of polynucleotides comprise a suitable dosage range or effective amount that provides any therapeutic effect. As appreciated by one of ordinary skill in the art a therapeutic effect depends on a number of factors, including the sequence of the polynucleotide, molecular weight of the polynucleotide and route of administration. Dosages are generally selected by the physician or other health care professional in accordance with a variety of parameters known in the art, such as severity of symptoms, history of the patient and the like. Generally, for a polynucleotide of about 20 bases, a dosage range may be selected from, for example, an independently selected lower limit such as about 0.1, 0.25, 0.5, 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400 or 500 mg/kg up to an independently selected upper limit, greater than the lower limit, of about 60, 80, 100, 200, 300, 400, 500, 750, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000 or 10,000 mg/kg. For example, a dose may be about any of the following: 0.1 to 100 mg/kg, 0.1 to 50 mg/kg, 0.1 to 25 mg/kg, 0.1 to 10 mg/kg, 1 to 500 mg/kg, 100 to 400 mg/kg, 200 to 300 mg/kg, 1 to 100 mg/kg, 100 to 200 mg/kg, 300 to 400 mg/kg, 400 to 500 mg/kg, 500 to 1000 mg/kg, 500 to 5000 mg/kg, or 500 to 10,000 mg/kg. Generally, parenteral routes of administration may require higher doses of polynucleotide compared to more direct application to the nucleotide to diseased tissue, as do polynucleotides of increasing length.

[0376] In one embodiment, human unit dose forms of T-cells comprise a suitable dosage range or effective amount that provides any therapeutic effect. As appreciated by one of ordinary skill in the art, a therapeutic effect depends on a number of factors. Dosages are generally selected by the physician or other health care professional in accordance with a variety of parameters known in the art, such as severity of symptoms, history of the patient and the like. A dose may be about 10⁴ cells to about 10⁶ cells, about 10⁶ cells to about 10⁸ cells, about 10⁸ to about 10¹¹ cells, or about 10⁸ to about 5×10¹⁰ cells. A dose may also about 10⁶ cells/m² to about 10¹⁰ cells/m², or about 10⁶ cells/m² to about 10⁸ cells/m².

[0377] Proteins(s) of the invention, and/or nucleic acids encoding the protein(s), can also be administered via liposomes, which may also serve to: 1) target the proteins(s) to a particular tissue, such as lymphoid tissue; 2) to target selectively to diseases cells; or, 3) to increase the half-life of the peptide composition. Liposomes include emulsions, foams, micelles, insoluble monolayers, liquid crystals, phospholipid dispersions, lamellar layers and the like. In these preparations, the peptide to be delivered is incorporated as part of a liposome, alone or in conjunction with a molecule which binds to a receptor prevalent among lymphoid cells, such as monoclonal antibodies which bind to the CD45 antigen, or with other therapeutic or immunogenic compositions Thus, liposomes either filled or decorated with a desired peptide of the invention can be directed to the site of lymphoid cells, where the liposomes then deliver the peptide compositions. Liposomes for use in accordance with the invention are formed from standard vesicle-forming lipids, which generally include neutral and negatively charged phospholipids and a sterol, such as cholesterol. The selection of lipids is generally guided by consideration of, e.g., liposome size, acid lability and stability of the liposomes in the blood stream. A variety of methods are available for preparing liposomes, as described in, e.g., Szoka, et al., Ann. Rev. Biophys. Bioeng. 9:467 (1980), and U.S. Pat. Nos. 4,235,871, 4,501,728, 4,837,028, and 5,019,369.

[0378] For targeting cells of the immune system, a ligand to be incorporated into the liposome can include, e.g., antibodies or fragments thereof specific for cell surface determinants of the desired immune system cells. A liposome suspension containing a peptide may be administered intravenously, locally, topically, etc. in a dose which varies according to, inter alia, the manner of administration, the peptide being delivered, and the stage of the disease being treated.

[0379] For solid compositions, conventional nontoxic solid carriers may be used which include, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharin, talcum, cellulose, glucose, sucrose, magnesium carbonate, and the like. For oral administration, a pharmaceutically acceptable nontoxic composition is formed by incorporating any of the normally employed excipients, such as those carriers previously listed, and generally 10-95% of active ingredient, that is, one or more peptides of the invention, and more preferably at a concentration of 25%-75%.

[0380] For aerosol administration, immunogenic peptides are preferably supplied in finely divided form along with a surfactant and propellant. Typical percentages of peptides are about 0.01%-20% by weight, preferably about 1%-10%. The surfactant must, of course, be nontoxic, and preferably soluble in the propellant. Representative of such agents are the esters or partial esters of fatty acids containing from about 6 to 22 carbon atoms, such as caproic, octanoic, lauric, palmitic, stearic, linoleic, linolenic, olesteric and oleic acids with an aliphatic polyhydric alcohol or its cyclic anhydride. Mixed esters, such as mixed or natural glycerides may be employed. The surfactant may constitute about 0.1%-20% by weight of the composition, preferably about 0.25-5%. The balance of the composition is ordinarily propellant. A carrier can also be included, as desired, as with, e.g., lecithin for intranasal delivery.

[0381] XI.) Diagnostic and Prognostic Embodiments of 121P1F1.

[0382] As disclosed herein, 121P1F1 polynucleotides, polypeptides, reactive cytotoxic T cells (CTL), reactive helper T cells (HTL) and anti-polypeptide antibodies are used in well known diagnostic, prognostic and therapeutic assays that examine conditions associated with dysregulated cell growth such as cancer, in particular the cancers listed in Table I (see, e.g., both its specific pattern of tissue expression as well as its overexpression in certain cancers as described for example in Example 4).

[0383] 121P1F1 can be analogized to a prostate associated antigen PSA, the archetypal marker that has been used by medical practitioners for years to identify and monitor the presence of prostate cancer (see, e.g., Merrill et al., J. Urol. 163(2): 503-5120 (2000); Polascik et al., J. Urol. Aug; 162(2):293-306 (1999) and Fortier et al., J. Nat. Cancer Inst. 91(19): 1635-1640(1999)). A variety of other diagnostic markers are also used in similar contexts including p53 and K-ras (see, e.g., Tulchinsky et al., Int J Mol Med July 1999 4(1):99-102 and Minimoto et al., Cancer Detect Prev 2000;24(1):1-12). Therefore, this disclosure of 121P1F1 polynucleotides and polypeptides (as well as 121P1F1 polynucleotide probes and anti-121P1F1 antibodies used to identify the presence of these molecules) and their properties allows skilled artisans to utilize these molecules in methods that are analogous to those used, for example, in a variety of diagnostic assays directed to examining conditions associated with cancer.

[0384] Typical embodiments of diagnostic methods which utilize the 121P1F1 polynucleotides, polypeptides, reactive T cells and antibodies are analogous to those methods from well-established diagnostic assays which employ, e.g., PSA polynucleotides, polypeptides, reactive T cells and antibodies. For example, just as PSA polynucleotides are used as probes (for example in Northern analysis, see, e.g., Sharief et al., Biochem. Mol. Biol. Int. 33(3):567-74(1994)) and primers (for example in PCR analysis, see, e.g., Okegawa etal., J. Urol. 163(4): 1189-1190 (2000)) to observe the presence and/or the level of PSA mRNAs in methods of monitoring PSA overexpression or the metastasis of prostate cancers, the 121P1F1 polynucleotides described herein can be utilized in the same way to detect 121P1F1 overexpression or the metastasis of prostate and other cancers expressing this gene. Alternatively, just as PSA polypeptides are used to generate antibodies specific for PSA which can then be used to observe the presence and/or the level of PSA proteins in methods to monitor PSA protein overexpression (see, e.g., Stephan et al., Urology 55(4):560-3 (2000)) or the metastasis of prostate cells (see, e.g., Alanen et al., Pathol. Res. Pract. 192(3):233-0.7 (1996)), the 121P1F1 polypeptides described herein can be utilized to generate antibodies for use in detecting 121P1F1 overexpression or the metastasis of prostate cells and cells of other cancers expressing This gene.

[0385] Specifically, because metastases involves the movement of cancer cells from an organ of origin (such as the lung or prostate gland etc.) to a different area of the body (such as a lymph node), assays which examine a biological sample for the presence of cells expressing 121P1F1 polynucleotides and/or polypeptides can be used to provide evidence of metastasis. For example, when a biological sample from tissue that does not normally contain 121P1F1-expressing cells (lymph node) is found to contain 121P1F1-expressing cells such as the 121P1F1 expression seen in LAPC4 and LAPC9, xenografts isolated from lymph node and bone metastasis, respectively, this finding is indicative of metastasis.

[0386] Alternatively 121P1F1 polynucleotides and/or polypeptides can be used to provide evidence of cancer, for example, when cells in a biological sample that do not normally express 121P1F1 or express 121P1F1 at a different level are found to express 121P1F1 or have an increased expression of 121P1F1 (see, e.g., the 121P1F1 expression in the cancers listed in Table I and in patient samples etc. shown in the accompanying Figures). In such assays, artisans may further wish to generate supplementary evidence of metastasis by testing the biological sample for the presence of a second tissue restricted marker (in addition to 121P1F1) such as PSA, PSCA etc. (see, e.g., Alanen et al., Pathol. Res. Pract. 192(3): 233-237 (1996)).

[0387] Just as PSA polynucleotide fragments and polynucleotide variants are employed by skilled artisans for use in methods of monitoring PSA, 121P1F1 polynucleotide fragments and polynucleotide variants are used in an analogous manner. In particular, typical PSA polynucleotides used in methods of monitoring PSA are probes or primers which consist of fragments of the PSA cDNA sequence. Illustrating this, primers used to PCR amplify a PSA polynucleotide must include less than the whole PSA sequence to function in the polymerase chain reaction. In the context of such PCR reactions, skilled artisans generally create a variety of different polynucleotide fragments that can be used as primers in order to amplify different portions of a polynucleotide of interest or to optimize amplification reactions (see, e.g., Caetano-Anolles, G. Biotechniques 25(3): 472-476, 478-480 (1998); Robertson et al., Methods Mol. Biol. 98:121-154 (1998)). An additional illustration of the use of such fragments is provided in Example 4, where a 121P1F1 polynucleotide fragment is used as a probe to show the expression of 121P1F1 RNAs in cancer cells. In addition, variant polynucleotide sequences are typically used as primers and probes for the corresponding mRNAs in PCR and Northern analyses (see, e.g., Sawai et al., Fetal Diagn. Ther. November-December 1996 11(6):407-13 and Current Protocols In Molecular Biology, Volume 2, Unit 2, Frederick M. Ausubel et al. eds., 1995)). Polynucleotide fragments and variants are useful in this context where they are capable of binding to a target polynucleotide sequence (e.g., a 121P1F1 polynucleotide shown in FIG. 2 or variant thereof) under conditions of high stringency.

[0388] Furthermore, PSA polypeptides which contain an epitope that can be recognized by an antibody or T cell that specifically binds to that epitope are used in methods of monitoring PSA. 121P1F1 polypeptide fragments and polypeptide analogs or variants can also be used in an analogous manner. This practice of using polypeptide fragments or polypeptide variants to generate antibodies (such as anti-PSA antibodies or T cells) is typical in the art with a wide variety of systems such as fusion proteins being used by practitioners (see, e.g., Current Protocols In Molecular Biology, Volume 2, Unit 16, Frederick M. Ausubel et al. eds., 1995). In this context, each epitope(s) functions to provide the architecture with which an antibody or T cell is reactive. Typically, skilled artisans create a variety of different polypeptide fragments that can be used in order to generate immune responses specific for different portions of a polypeptide of interest (see, e.g., U.S. Pat. Nos. 5,840,501 and 5,939,533). For example it may be preferable to utilize a polypeptide comprising one of the 121P1F1 biological motifs discussed herein or a motif-bearing subsequence which is readily identified by one of skill in the art based on motifs available in the art. Polypeptide fragments, variants or analogs are typically useful in this context as long as they comprise an epitope capable of generating an antibody or T cell specific for a target polypeptide sequence (e.g. a 121P1F1 polypeptide shown in FIG. 3).

[0389] As shown herein, the 121P1F1 polynucleotides and polypeptides (as well as the 121P1F1 polynucleotide probes and anti-121P1F1 antibodies or T cells used to identify the presence of these molecules) exhibit specific properties that make them useful in diagnosing cancers such as those listed in Table I. Diagnostic assays that measure the presence of 121P1F1 gene products, in order to evaluate the presence or onset of a disease condition described herein, such as prostate cancer, are used to identify patients for preventive measures or further monitoring, as has been done so successfully with PSA. Moreover, these materials satisfy a need in the art for molecules having similar or complementary characteristics to PSA in situations where, for example, a definite diagnosis of metastasis of prostatic origin cannot be made on the basis of a test for PSA alone (see, e.g., Alanen et al., Pathol. Res. Pract. 192(3): 233-237 (1996)), and consequently, materials such as 121P1F1 polynucleotides and polypeptides (as well as the 121P1F 1 polynucleotide probes and anti-121P1F 1 antibodies used to identify the presence of these molecules) need to be employed to confirm a metastases of prostatic origin.

[0390] Finally, in addition to their use in diagnostic assays, the 121P1F1 polynucleotides disclosed herein have a number of other utilities such as their use in the identification of oncogenetic associated chromosomal abnormalities in the chromosomal region to which the 121P1F1 gene maps (see Example 3 below). Moreover, in addition to their use in diagnostic assays, the 121P1F1-related proteins and polynucleotides disclosed herein have other utilities such as their use in the forensic analysis of tissues of unknown origin (see, e.g., Takahama K Forensic Sci hit Jun. 28, 1996;80(1-2): 63-9).

[0391] Additionally, 121P1F1-related proteins or polynucleotides of the invention can be used to treat a pathologic condition characterized by the over-expression of 121P1F1. For example, the amino acid or nucleic acid, sequence of FIG. 2 or FIG. 3, or fragments of either, can be used to generate an immune response to a 121P1F1 antigen. Antibodies or other molecules that react with 121P1F1 can be used to modulate the function of this molecule, and thereby provide a therapeutic benefit.

[0392] XII.) Inhibition of 121P1F1 Protein Function

[0393] The invention includes various methods and compositions for inhibiting the binding of 121P1F 1 to its binding partner or its association with other protein(s) as well as methods for inhibiting 121P1F1 function.

[0394] XII.A.) Inhibition of 121P1F1 With Intracellular Antibodies

[0395] In one approach, a recombinant vector that encodes single chain antibodies that specifically bind to 121P1F1 are introduced into 121P1F1 expressing cells via gene transfer technologies. Accordingly, the encoded single chain anti-121P1F1 antibody is expressed intracellularly, binds to 121P1F1 protein, and thereby inhibits its function. Methods for engineering such intracellular single chain antibodies are well known. Such intracellular antibodies, also known as “intrabodies”, are specifically targeted to a particular compartment within the cell, providing control over where the inhibitory activity of the treatment is focused. This technology has been successfully applied in the art (for review, see Richardson and Marasco, 1995, TIBTECH vol. 13). Intrabodies have been shown to virtually eliminate the expression of otherwise abundant cell surface receptors (see, e.g., Richardson et al., 1995, Proc. Natl. Acad. Sci. USA 92: 3137-3141; Beerli et al., 1994, J. Biol. Chem. 289: 23931-23936; Deshane et al., 1994, Gene Ther. 1: 332-337).

[0396] Single chain antibodies comprise the variable domains of the heavy and light chain joined by a flexible linker polypeptide, and are expressed as a single polypeptide. Optionally, single chain antibodies are expressed as a single chain variable region fragment joined to the light chain constant region. Well-known intracellular trafficking signals are engineered into recombinant polynucleotide vectors encoding such single chain antibodies in order to precisely target the intrabody to the desired intracellular compartment. For example, intrabodies targeted to the endoplasmic reticulum (ER) are engineered to incorporate a leader peptide and, optionally, a C-terminal ER retention signal, such as the KDEL amino acid motif. Intrabodies intended to exert activity in the nucleus are engineered to include a nuclear localization signal. Lipid moieties are joined to intrabodies in order to tether the intrabody to the cytosolic side of the plasma membrane. Intrabodies can also be targeted to exert function in the cytosol. For example, cytosolic intrabodies are used to sequester factors within the cytosol, thereby preventing them from being transported to their natural cellular destination.

[0397] In one embodiment, intrabodies are used to capture 121P1F1 in the nucleus, thereby preventing its activity within the nucleus. Nuclear targeting signals are engineered into such 121P1F1 intrabodies in order to achieve the desired targeting. Such 121P1F1 intrabodies are designed to bind specifically to a particular 121P1F1 domain. In another embodiment, cytosolic intrabodies that specifically bind to a 121P1F1 protein are used to prevent 121P1F1 from gaining access to the nucleus, thereby preventing it from exerting any biological activity within the nucleus (e.g., preventing 121P1F1 from forming transcription complexes with other factors).

[0398] In order to specifically direct the expression of such intrabodies to particular cells, the transcription of the intrabody is placed under the regulatory control of an appropriate tumor-specific promoter and/or enhancer. In order to target intrabody expression specifically to prostate, for example, the PSA promoter and/or promoter/enhancer can be utilized (See, for example, U.S. Pat. No. 5,919,652 issued Jul. 6, 1999).

[0399] XII.B.) Inhibition of 121P1F1 with Recombinant Proteins

[0400] In another approach, recombinant molecules bind to 121P1F1 and thereby inhibit 121P1F1 function. For example, these recombinant molecules prevent or inhibit 121P1F1 from accessing/binding to its binding partner(s) or associating with other protein(s). Such recombinant, molecules can, for example, contain the reactive part(s) of a 121P1F1 specific antibody molecule. In a particular embodiment, the 121P1F1 binding domain of a 121P1F1 binding partner is engineered into a dimeric fusion protein, whereby the fusion protein comprises two 121P1F1 ligand binding domains linked to the Fc portion of a human IgG, such as human IgG1. Such IgG portion can contain, for example, the C_(H)2 and C_(H)3 domains and the hinge region, but not the C_(H)1 domain. Such dimeric fusion proteins are administered in soluble form to patients suffering from a cancer associated with the expression of 121P1F1, whereby the dimeric fusion protein specifically binds to 121P1F1 and blocks 121P1F1 interaction with a binding partner. Such dimeric fusion proteins are further combined into multimeric proteins using known antibody linking technologies.

[0401] XII.C.) Inhibition of 121P1F1 Transcription or Translation

[0402] The present invention also comprises various methods and compositions for inhibiting the transcription of the 121P1F1 gene. Similarly, the invention also provides methods and compositions for inhibiting the translation of 121P1F1 mRNA into protein.

[0403] In one approach, a method of inhibiting the transcription of the 121P1F1 gene comprises contacting the 121P1F1 gene with a 121P1F1 antisense polynucleotide. In another approach, a method of inhibiting 121P1F1 mRNA translation comprises contacting a 121P1F1 mRNA with an antisense polynucleolide. In another approach, a 121P1F1 specific ribozyme is used to cleave a 121P1F1 message, thereby inhibiting translation. Such antisense and ribozyme based methods can also be directed to the regulatory regions of the 121P1F1 gene, such as 121P1F1 promoter and/or enhancer elements. Similarly, proteins capable of inhibiting a 121P1F1 gene transcription factor are used to inhibit 121P1F1 mRNA transcription. The various polynucleotides and compositions useful in the aforementioned methods have been described above. The use of antisense and ribozyme molecules to inhibit transcription and translation is well known in the art.

[0404] Other factors that inhibit the transcription of 121P1F1 by interfering with 121P1F1 transcriptional activation are also useful to treat cancers expressing 121P1F1. Similarly, factors that interfere with 121P1F1 processing are useful to treat cancers that express 121P1F1. Cancer treatment methods utilizing such factor; are also within the scope of the invention.

[0405] XII.D.) General Considerations for Therapeutic Strateyies

[0406] Gene transfer and gene therapy technologies can be used to deliver therapeutic polynucleotide molecules t: tumor cells synthesizing 121P1F1 (i.e., antisense, ribozyme, polynucleotides encoding intrabodies and other 121P1F1 inhibitory molecules). A number of gene therapy approaches are known in the art. Recombinant vectors encoding 121P1F1 antisense polynucleotides, ribozymes, factors capable of interfering with 121P1F1 transcription, and so forth, can be delivered to target tumor cells using such gene therapy approaches.

[0407] The above therapeutic approaches can be combined with any one of a wide variety of surgical, chemotherapy or radiation therapy regimens. The therapeutic approaches of the invention can enable the use of reduced dosages of chemotherapy (or other therapies) and/or less frequent administration, an advantage for all patients and particularly for those that do not tolerate the toxicity of the chemotherapeutic agent well.

[0408] The anti-tumor activity of a particular composition (e.g., antisense, ribozyme, intrabody), or a combination of such compositions, can be evaluated using various in vitro and in vivo assay systems. In vitro assays that evaluate therapeutic activity include cell growth assays, soft agar assays and other assays indicative of tumor promoting activity, binding assays capable of determining the extent to which a therapeutic composition will inhibit the binding of 121P1F1 to a binding partner, etc.

[0409] In vivo, the effect of a 121P1F1 therapeutic composition can be evaluated in a suitable animal model. For example, xenogenic prostate cancer models can be used, wherein human prostate cancer explants or passaged xenograft tissues are introduced into immune compromised animals, such as nude or SCID mice (Klein et al., 1997, Nature Medicine 3: 402-408). For example, PCT Patent Application WO98/16628 and U.S. Pat. No. 6,107,540 describe various xenograft models of human prostate cancer capable of recapitulating the development of primary tumors, micrometastasis, and the formation of osteoblastic metastases characteristic of late stage disease. Efficacy can be predicted using assays that measure inhibition of tumor formation, tumor regression or metastasis, and the like.

[0410] In vivo assays that evaluate the promotion of apoptosis are useful in evaluating therapeutic compositions. In one embodiment, xenografts from tumor bearing mice treated with the therapeutic composition can be examined for the presence of apoptotic foci and compared to untreated control xenograft-bearing mice. The extent to which apoptotic foci are found in the tumors of the treated mice provides an indication of the therapeutic efficacy of the composition.

[0411] The therapeutic compositions used in the practice of the foregoing methods can be formulated into pharmaceutical compositions comprising a carrier suitable for the desired delivery method. Suitable carriers include any material that when combined with the therapeutic composition retains the anti-tumor function of the therapeutic composition and is generally non-reactive with the patient's immune system. Examples include, but are not limited to, any of a number of standard pharmaceutical carriers such as sterile phosphate buffered saline solutions, bacteriostatic water, and the like (see, generally, Remington's Pharmaceutical Sciences 16^(th) Edition, A. Osal., Ed., 1980).

[0412] Therapeutic formulations can be solubilized and administered via any route capable of delivering the therapeutic composition to the tumor site. Potentially effective routes of administration include, but are not limited to, intravenous, parenteral, intraperitoneal, intramuscular, intratumor, intradermal, intraorgan, orthotopic, and the like. A preferred formulation for intravenous injection comprises the therapeutic *composition in a solution of preserved bacteriostatic water, sterile unpreserved water, and/or diluted in polyvinylchloride or polyethylene bags containing 0.9% sterile Sodium Chloride for Injection, USP. Therapeutic: protein preparations can be lyophilized and stored as sterile powders, preferably under vacuum, and then reconstituted in bacteriostatic water (containing for example, benzyl alcohol preservative) or in sterile water prior to injection.

[0413] Dosages and administration protocols for the treatment of cancers using the foregoing methods will vary with the method and the target cancer, and will generally depend on a number of other factors appreciated in the art.

[0414] XIII.) Kits

[0415] For use in the diagnostic and therapeutic applications described herein, kits are also within the scope of the invention. Such kits can comprise a carrier, package or container that is compartmentalized to receive one or more containers such as vials, tubes, and the like, each of the container(s) comprising one of the separate elements to be used in the method. For example, the container(s) can comprise a probe that is or can be detectably labeled. Such probe can be an antibody or polynucleotide specific for a 121P1F1-related protein or a 121P1F1 gene or message, respectively. Where the method utilizes nucleic acid hybridization to detect the target nucleic acid, the kit can also have containers containing nucleotide(s) for amplification of the target nucleic acid sequence and/or a container comprising a reporter-means, such as a biotin-binding protein, such as avidin or streptavidin, bound to a reporter molecule, such as an enzymatic, florescent, or radioisotope label. The kit can include all or part of the amino acid sequence of FIG. 2 or FIG. 3 or analogs thereof, or a nucleic acid molecules that encodes such amino acid sequences.

[0416] The kit of the invention will typically comprise the container described above and one or more other containers comprising materials desirable from a commercial and user standpoint, including buffers, diluents, filters, needles, syringes, and package inserts with instructions for use.

[0417] A label can be present on the container to indicate that the composition is used for a specific therapy or non-therapeutic application, and can also indicate directions for either in vivo or in vitro use, such as those described above. Directions and or other information can also be included on an insert which is included with the kit.

EXAMPLES

[0418] Various aspects of the invention are further described and illustrated by way of the several examples that follow, none of which are intended to limit the scope of the invention.

Example 1 SSH-Generated Isolation of a cDNA Fragment of the 121P1F1 Gene

[0419] Suppression Subtractive Hybridization (SSH) was used to identify cDNAs corresponding to genes that are differentially expressed in prostate cancer. The SSH reaction utilized cDNA from two LAPC-9 AD xenografts. Specifically, to isolate genes that are involved in the progression of androgen dependent (AD) prostate cancer to androgen independent (AI) cancer, the LAPC-9 AD xenograft in male SCID mice was used. Mice that harbored LAPC-9 AD xenografts were castrated when the tumors reached a size of 1 cm in diameter. The tumors regressed in size and temporarily stopped producing the androgen dependent protein PSA. Seven to fourteen days post-castration, PSA levels were detectable again in the blood of the mice. Eventually the tumors develop an AI phenotype and start growing again in the castrated males. Tumors were harvested at different time points after castration to identify genes that were turned on or off during the transition to androgen independence.

[0420] The gene 121P1F1 was derived from an LAPC-9 AD minus LAPC-9 AD (28 days post-castration) subtraction. The SSH DNA sequence of 254 bp (FIG. 1) is novel and did not exhibit significant homology to any known human genes in public databases.

[0421] The 121P1F1 SSH cDNA of 254 bp is listed in FIG. 1. The full length 121P1F1 cDNAs and ORFs are described in FIG. 2 with the protein sequences listed in FIG. 3.

[0422] Materials and Methods

[0423] LAPC Xenografts and Human Tissues:

[0424] LAPC xenografts were obtained from Dr. Charles Sawyers (UCLA) and generated as described (Klein et al, 1997, Nature Med. 3: 402-408; Craft et al., 1999, Cancer Res. 59: 5030-5036). Androgen dependent and independent LAPC-4 xenografts LAPC-4 AD and AI, respectively) and LAPC-9 AD and AI xenografts were grown in male SCID mice and were passaged as small tissue chunks in recipient males. LAPC-4 and -9 AI xenografts were derived from LAPC-4 or -9 AD tumors, respectively. To generate the AI xenografts, male mice bearing AD tumors were castrated and maintained for 2-3 months. After the tumors re-grew, the tumors were harvested and passaged in castrated males or in female SCID mice.

[0425] RNA Isolation:

[0426] Tumor tissues were homogenized in Trizol reagent (Life Technologies, Gibco BRL) using 10 ml/g tissue or 10 ml/10⁸ cells to isolate total RNA. Poly A RNA was purified from total RNA using Qiagen's Oligotex mRNA Mini and Midi kits. Total and mRNA were quantified by spectrophotometric analysis (O.D. 260/280 nm) and analyzed by gel electrophoresis.

[0427] Oligonucleotides:

[0428] The following HPLC purified oligonucleotides were used. DPNCDN (cDNA synthesis primer): (SEQ ID NO: 714) 5′TTTTGATCAAGCTT₃₀3′ Adaptor 1: (SEQ ID NO: 715) 5′CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAG3′ (SEQ ID NO: 716) 3′GGCCCGTCCTAG5′ Adaptor 2: (SEQ ID NO: 717) 5′GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG3′ (SEQ ID NO: 718) 3′CGGCTCCTAG5′ PCR primer 1: (SEQ ID NO: 719) 5′CTAATACGACTCACTATAGGGC3′ Nested primer (NP)1: (SEQ ID NO: 720) 5′TCGAGCGGCCGCCCGGGCAGGA3′ Nested primer (NP)2: (SEQ ID NO: 721) 5′AGCGTGGTCGCGGCCGAGGA3′

[0429] Suppression Subtractive Hybridization:

[0430] Suppression Subtractive Hybridization (SSH) was used to identify cDNAs corresponding to genes that may be differentially expressed in prostate cancer. The SSH reaction utilized cDNA from prostate cancer xenograft LAPC-9AD. The gene 121P1F1 was derived from an LAPC-9 AD minus LAPC-9 AD (28 days post-castration) subtraction. The SSH DNA sequence (FIG. 1) was identified.

[0431] The cDNA derived from prostate cancer xenograft LAPC-9AD tissue was used as the source of the “driver” cDNA, while the cDNA from prostate cancer xenograft LAPC-9AD (28 days post-castration) was used as the source of the “tester” cDNA. Double stranded cDNAs corresponding to tester and driver cDNAs were synthesized from 2 μg of poly(A)+ RNA isolated from the relevant tissue, as described above, using CLONTECH's PCR-Select cDNA Subtraction Kit and 1 ng of oligonucleotide DPNCDN as primer. First- and second-strand synthesis were carried out as described in the Kit's user manual protocol (CLONTECH Protocol No. PT1117-1, Catalog No. K1804-1). The resulting cDNA was digested with Dpn II for 3 hrs at 37° C. Digested cDNA was extracted with phenol/chloroform (1:1) and ethanol precipitated.

[0432] Tester cDNA was generated by diluting 1 μl of Dpn II digested cDNA from the relevant tissue source (see above) (400 ng) in 5 μl of water. The diluted cDNA (2 μl, 160 ng) was then ligated to 2 μl of Adaptor 1 and Adaptor 2 (10 μM), in separate ligation reactions, in a total volume of 10 μl at 16° C. overnight, using 400 U of T4 DNA ligase (CLONTECH). Ligation was terminated with 1 μl of 0.2 M EDTA and heating at 72° C. for 5 min.

[0433] The first hybridization was performed by adding 1.5 μl (600 ng) of driver cDNA to each of two tubes containing 1.5 μl (20 ng) Adaptor 1- and Adaptor 2-ligated tester cDNA. In a final volume of 4 p¹, the samples were overlaid with mineral oil, denatured in an MJ Research thermal cycler at 98° C. for 1.5 minutes, and then were allowed to hybridize for 8 hrs at 68° C. The two hybridizations were then mixed together with an additional 1 μl of fresh denatured driver cDNA and were allowed to hybridize overnight at 68° C. The second hybridization was then diluted in 200 μl of 20 mM Hepes, pH 8.3, 50 mM NaCl, 0.2 mM EDTA, heated at 70° C. for 7 min. and stored at −20° C.

[0434] PCR Amplification, Cloning and Sequencing of Gene Fragments Generated from SSH:

[0435] To amplify gene fragments resulting from SSH reactions, two PCR amplifications were performed. In the primary PCR reaction 1 μl of the diluted final hybridization mix was added to 1 μl of PCR primer 1 (10 μM), 0.5 μl dNTP mix (10 μM), 2.5 μl 10× reaction buffer (CLONTECH) and 0.5 μl 50× Advantage cDNA polymerase Mix (CLONTECH) in a final volume of 25 μl. PCR 1 was conducted using the following conditions: 75° C. for 5 min., 94° C. for 25 sec., then 27 cycles of 94° C. for 10 sec, 66° C. for 30 sec, 72° C. for 1.5 min. Five separate primary PCR reactions were performed for each experiment. The products were pooled and diluted 1:10 with water. For the secondary PCR reaction, 1 μl from the pooled and diluted primary PCR reaction was added to the same reaction mix as used for PCR 1, except that primers NP1 and NP2 (10 μM) were used instead of PCR primer 1. PCR 2 was performed using 10-12 cycles of 94° C. for 10 sec, 68° C. for 30 sec, and 72° C. for 1.5 minutes. The PCR products were analyzed using 2% agarose gel electrophoresis.

[0436] The PCR products were inserted into pCR2.1 using the T/A vector cloning kit (Invitrogen). Transformed E. coli were subjected to blue/white and ampicillin selection. White colonies were picked and arrayed into 96 well plates and were grown in liquid culture overnight. To identify inserts, PCR amplification was performed on 1 ml of bacterial culture using the conditions of PCR1 and NP1 and NP2 as primers. PCR products were analyzed using 2% agarose gel electrophoresis.

[0437] Bacterial clones were stored in 20% glycerol in a 96 well format. Plasmid DNA was prepared, sequenced, and subjected to nucleic acid homology searches of the GenBank, dBest, and NCI-CGAP databases.

[0438] RT-PCR Expression Analysis:

[0439] First strand cDNAs can be generated from 1 μg of mRNA with oligo (dT)12-18 priming using the Gibco-BR1, Superscript Preamplification system. The manufacturer's protocol was used which included an incubation for 50 min at 42° C. with reverse transcriptase followed by RNAse H treatment at 37° C. for 20 min. After completing the reaction, the volume can be increased to 200 μl with water prior to normalization.

[0440] Normalization of the first strand cDNAs from multiple tissues was performed by using the primers 5′atatcgccgcgctcgtcgtcgacaa 3′ (SEQ ID NO: 722) and 5′agccacacgcagctcattgtagaagg 3′ (SEQ ID NO: 723) to amplify β-actin. First strand cDNA (5 μl) were amplified in a total volume of 50 μl containing 0.4 μM primers, 0.2 μM each dNTPs, 1× PCR buffer (Clontech, 10 MM Tris-HCL, 1.5 MM MgCl₂, 50 mM KCl, pH 8.3) and 1× Klentaq DNA polymerase (Clontech). Five μl of the PCR reaction can be removed at 18, 20, and 22 cycles and used for agarose gel electrophoresis. PCR was performed using an MJ Research thermal cycler under the following conditions: Initial denaturation can be at 94° C. for 15 sec, followed by a 18, 20, and 22 cycles of 94° C. for 15, 65° C. for 2 min, 72° C. for 5 sec. A final extension at 72° C. was carried out for 2 min. After agarose gel electrophoresis, the band intensities of the 283 bp β-actin bands from multiple tissues were compared by visual inspection. Dilution factors for the first strand cDNAs were calculated to result in equal β-actin band intensities in all tissues after 22 cycles of PCR. Three rounds of normalization can be required to achieve equal band intensities in all tissues after 22 cycles of PCR.

[0441] To determine expression levels of the 121P1F1 gene, 5 μl of normalized first strand cDNA were analyzed by PCR using 26, and 30 cycles of amplification. Semi-quantitative expression analysis can be achieved by comparing the PCR products at cycle numbers that give light band intensities. A typical RT-PCR expression analysis is shown in FIG. 17. RT-PCR expression analysis was performed on first strand cDNAs generated using pools of tissues from multiple samples. The cDNAs were shown to be normalized using beta-actin primers. PCR Expression was observed in human testis, prostate cancer xenografts, colon cancer tissue pools, lung cancer tissue pools, kidney cancer tissue pools, bladder cancer tissue pools, and prostate cancer tissue pools.

Example 2 Full Length Cloning of 121P1F1 and Homology Comparison to Known Sequences

[0442] To isolate genes that are involved in the progression of androgen dependent (AD) prostate cancer to androgen independent (AI) cancer, an experiment was conducted with the LAPC-9AD xenograft in male SCID mice. Mice that harbored LAPC-9AD xenografts were castrated when the tumors reached a size of 1 cm in diameter. The tumors regressed in size and temporarily stopped producing the androgen dependent protein PSA. Seven to fourteen days post-castration, PSA levels were detectable again in the blood of the mice. Eventually the tumors develop an AI phenotype and start growing again in the castrated males. Tumors were harvested at different time points after castration to identify genes that are turned on or off during the transition to androgen independence.

[0443] The gene 121P1F1 was derived from an LAPC-9AD minus LAPC-9AD (28 days post-castration) subtraction. The SSH DNA sequence of 254 bp (FIG. 1) is novel and did not exhibit significant homology to any known human genes in public databases.

[0444] A cDNA (clone A) of 863 bp was isolated from a Human Testis cDNA library, revealing an ORF of 205 amino acids (FIG. 2 and FIG. 3). It is probable that 121P1F1 is a cytoplasmic protein based on two topology algorithms (J. Mol. Biol. 2000, 300:1005 and Bioinformatics, 1998, 14:378) and based on its homology to Dynactin. However, it is also possible that 121P1F1 is localized in the nucleus based on PSORT analysis (http://psort.nibb.ac.jp:8800/form.html).

[0445] Sequence analysis of 121P1F1 reveals highest homology to human GAJ protein (FIG. 4C); the two proteins are 100% homologous over a 205 amino acid region. 121P1F1 also displays homology to a mouse putative protein (FIG. 4D). The two proteins are 89% identical over a 202 amino acid region. Also, 121P1F1 shows 40% identity over a 202 amino acid region with the 24.2 kDa hypothetical coiled-coil protein of fission yeast. (FIG. 4E)

[0446] The 121P1F1 cDNA was deposited on Mar. 1, 2001 with the American Type Culture Collection (ATCC; Manassas, Va.), and has been assigned Accession No. PTA-3139.

Example 3 Chromosomal Localization

[0447] Chromosomal localization can implicate genes in disease pathogenesis. Several chromosome mapping approaches are available, including fluorescent in situ hybridization (FISH), human/hamster radiation hybrid (RH) panels (Walter et al., 1994; Nature Genetics 7:22; Research Genetics, Huntsville Al), human-rodent somatic cell hybrid panels such as is available from the Coriell Institute (Camden, N.J.), and genomic viewers utilizing BLAST homologies to sequenced and mapped genomic clones (NCBI, Bethesda, Md.).

[0448] 121P1F1 maps to chromosome 4q, using 121P1F1 sequence and the NCBI BLAST tool: (http://wwv.ncbi.nlm.nih.gov/genome/seq/page.cgi?F=HsBlast.html&&ORG=Hs).

Example 4 Expression Analysis of 121P1F1 in Normal Tissues and Patient Specimens

[0449] Expression analysis by RT-PCR demonstrated that 121P1F1 expression is reminiscent of a cancer-testis gene (FIG. 17A). Normal tissue expression is restricted to testis and, to a lower extent, it is detected in the thymus and ovary. Analysis of human patient cancer RNA pools shows expression in prostate, kidney, and bladder cancers, as well as lung cancers (FIG. 17B).

[0450] Extensive Northern blot analysis of 121P1F1 in 16 human normal tissues confirmed the expression observed by RT-PCR (FIG. 18). A 1.2 kb transcript was detected in testis and at lower levels in thymus. 121P1F1 expression was also shown in prostate cancer xenografts and in all cancer cell lines tested, such as in prostate (LAPC 4AD, LAPC 4AI, LAPC 9AD, LAPC 9AI, LNCaP, PC-3, DU145 Tsu-Pr1, and LAPC4); bladder (HT1197, SCaBER, UM-UC-3, TCCSUP, J82, 5637), lung (A427, NCI-H82, NCI-H146), kidney (769-P, A-498, CAKI-1, SW 839), pancreas (PANC-1, Bx PC-3, HPAC, Capan-1); colon (SK-CO-1, Caco-2, LoVo, T84, Colo205) and in the cancer cell lines 293T, RD-ES and KCL22.(FIG. 19). These results indicated that 121P1F1 is a testis specific gene that is upregulated in cancers.

[0451] Northern blot analysis showed that 121P1F1 is expressed in prostate tumor tissues derived from prostate cancer patients (FIG. 20). It was also expressed in kidney, cervix, breast and stomach patient cancer samples (FIG. 21). The expression detected in normal adjacent tissues (isolated from diseased tissues) but not in normal tissues, isolated from healthy donors, indicate that these tissues are not fully normal and that 121P1F1 is expressed in early stage tumors, and thus can be used as a diagnostic target.

[0452] Since 121P1F1 was derived from a LAPC-9 AD minus LAPC-9 AD (28 days post-castration) subtraction, an assay was performed for androgen regulation of 121P1F1 (FIG. 22). LAPC-4 cells were grown in charcoal-stripped medium and stimulated with the synthetic androgen mibolerone, for either 14 or 24 hours. It was shown that the expression of 121P1F1 went down in absence of normal serum, and is modulated:m presence of mibolerone, 24 hours after stimulation. The experimental samples were confirmed by testing for the expression of the androgen-regulated prostate cancer gene TMPRSS2. This experiment showed that, as expected, TMPRSS2 levels go down in presence of charcoal-stripped serum, and expression is induced at 14 and 24 hours in presence of mibolerone.

[0453]FIG. 15 shows androgen regulation of 121P1F1 in vivo. Male mice were injected with LAPC-9AD tumor cells. When tumor reached a palpable size (0.3-0.5 cm in diameter), mice were castrated and tumors harvested at different time points following castration. RNA was isolated from the xenograft tissues. Northern blots with 10 μg of total RNA/lane were probed with the 121P1F1 SSH fragment; size standards in kilobases (kb) are indicated on the side. Results show that expression of 121P IF 1 is slightly downregulated 7 days after castration. The protein TMPRSS2 was used as a positive control. A picture of the ethidium-bromide staining of the RNA gel is also presented (lowest panel).

[0454] 121P1F1 expression is reminiscent of a cancer-testis gene. Its restricted normal tissue expression and the upregulation detected in prostate cancer, bladder cancer, kidney cancer, colon cancer, and lung cancer, indicate that 121P1F1 is therapeutic and prophylactic target and a diagnostic and prognostic marker for human cancers.

Example 5 Splice Variants of 121P1F1 and Single Nucleotide Polymorphisms Splice Variants

[0455] Splice variants are alternatively spliced transcripts. When a gene is transcribed from genomic DNA, the initial RNA is generally spliced to produce functional mRNA, which has only exons and is used for translation into an amino acid sequence. Accordingly, a given gene can have zero to many alternatively spliced mRNA products. Alternative transcripts each have a unique exon makeup, and can have different coding and/Dr non-coding (5′ or 3′ end) portions, from the original transcript. Alternative transcripts can code for similar proteins with the same or a similar function or may encode proteins with different functions, and may be expressed in the same tissue at the same time, or at different tissue at different times. Proteins encoded by alternative transcripts can have similar or different cellular or extracellular localizations, e.g., be secreted.

[0456] Splice variants are identified by a variety of art-accepted methods. For example, splice variants are identified by use of EST data. First, all human ESTs were grouped into clusters which show direct or indirect identity with each other. Second, ESTs in the same cluster were further grouped into sub-clusters and assembled into a consensus sequence. The starting gene is compared to the consensus sequence(s). Each consensus sequence is a potential splice variant for that gene (see, e.g., Web URL www.doubletwist.com/products/c11_agentsOverview.jhtml). Even when a variant is identified that is not a fill-length clone, that portion of the variant is very useful for antigen generation and for further cloning of the full-length splice variant, using techniques known in the art.

[0457] Moreover, computer programs are available in the art that identify splice variants based on genomic sequences. Genomic-based variant identification programs include FgenesH (A. Salamov and V. Solovyev, “Ab initio gene finding in Drosophila genomic DNA,” Genome Research. April 2000; 10(4):516-22); Grail (Web URL compbio.oml.gov/Grail-bin/EmtyGrailForm) and GenScan (Web URL genes.mit.edu/GENSCAN.html). For a general discussion of splice variant identification protocols see., e.g., Southan C., “A genomic perspective on human proteases,” FEBS Lett. (Jun. 8, 2001) 498(2-3):214-8; and de Soima SJ, et al., “Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags,” Proc. Natl. Acad. Sci. USA. (2000 Nov 7) 97(23): 12690-3.

[0458] For variants identified by the EST-based method, Table XXII shows the nucleotide sequences of the splice variants. Table XXIII shows the alignment of the splice variant with the 121P1F1 nucleic acid sequence. Table XXIV displays alignments of an amino acid sequence encoded by a splice variant with 121P1F1 v.1. Table XXV lays out the amino acid translation of the splice variant for the identified reading frame orientation. Tables XXII through XXV are set forth herein on a variant-by-variant basis.

[0459] For variants identified by any one of the genomic sequence-based methods, Table XXII shows the nucleotide sequences of the splice variant. Table XXIII shows the alignment of the splice variant with the 121P1F1 nucleic acid sequence. Table XXIV displays the alignment of amino acid sequence of the predicted transcripts with 121P1F1. The genomic-based computer programs predict a transcript from genomic sequence, and not only predict exons but also set open reading frame as the first forward open reading frame. The predicted transcript does not contain 5′ or 3′ untranslated region (UTR). It starts with ATG and ends with a stop codon, TAG, TGA or TAA. In case the transcript is predicted on the reverse strand of the genomic sequence, the sequence of the transcript is reverse-complemented to the genomic sequence of the exons. Thus, the genomic-based programs provide the correct transcript sequence, with 5′ to 3′ orientation and +1 as the open reading frame. However, due to the possibility of inaccurate prediction of exons or the possibility of sequencing errors in genomic data, other peptides in other forward open reading frames can also be encoded by the variant.

[0460] To further confirm the parameters of a splice variant, a variety of techniques are available in the art, such as full-length cloning, proteomic validation, PCR-based validation, and 5′ RACE validation, etc. (see e.g., Proteomic Validation: Brennan S O, Fellowes A P, George P M.; “Albumin banks peninsula: a new termination variant characterised by electrospray mass spectrometry.” Biochim Biophys Acta. Aug. 17, 1999; 1433(1-2):321-6; Ferranti P, et al., “Differential splicing of pre-messenger RNA produces multiple forms of mature caprine alpha(s1)-casein.” Eur J. Biochem. Oct. 1, 1997; 249(1): 1-7; PCR-based Validation: Wellmann S. et al., “Specific reverse transcription-PCR quantification of vascular endothelial growth factor (VEGF) splice variants by LightCycler technology.” Clin Chem. April 2001; 47(4):654-60; Jia H P, et al., Discovery of new human beta-defensins using a genomics-based approach,” Gene. Jan. 24, 2001;263(1-2):211-8; PCR-based and 5′ RACE Validation: Brigle K E, et al., “Organization of the murine reduced folate carrier gene and identification of variant splice forms,” Biochim Biophys Acta. Aug. 7, 1997; 1353(2): 191-8.

[0461] It is known in the art that genomic regions are modulated in cancers. When the genomic region to which 1211P1F1 maps is modulated in a particular cancer, the splice variants of 121P1F1 are modulated as well. Disclosed herein is that 12P1F1 has a particular expression profile. Splice variants of 121P1F1 that are structurally and/or functionally similar to 121P1F1 share this expression pattern, thus serving as tumor-associated markers/antigens.

[0462] Using the EST assembly approach, we identified four splice variants. They were designated as splice variant 1 to 4. Splice variant 1 has two potential open reading frames and thus two potential translated peptide sequences, designated as 1A and 1B.

[0463] Single Nucleotide Polymorphisms (SNPs)

[0464] A Single Nucleotide Polymorphism (SNP) is a single base pair variation in a nucleotide sequence. As appreciated by those in the art, in a single nucleotide cange in a codon can cuse the codon to encode a different amino acid. Thus a SNP can change amino acids of the protein encoded by the gene and thus change the functions of the protein. Some SNPs cause inherited diseases and some others contribute to quantitative variations in phenotype and reactions to environmental factors including diet and drugs among individuals. Therefore, the occurance of one or more SNPs is relevant in many contexts, including but not limited to diagnosis of inherited or acquired disease, determination of drug reactions and dosage, identification of genes responsible for disearse and discovery of the genetic relationship between individuals (P. Nowotny, J. M. Kwon and A. M. Goate, “SNP analysis to dissect human traits,” Curr. Opin. Neurobiol. 2001 October; 11(5):637-641; M. Pirmohamed and B. K. Park, “Genetic susceptibility to adverse drug reactions,” Trends Pharmacol. Sci. June 2001; 22(6):298-305; J. H. Riley, C. J. Allan, E. Lai and A. Roses, “The use of single nucleotide polymorphisms in the isolation of common disease genes,” Pharmacogenomics. February 2000; 1(1):39-47; R. Judson, J. C. Stephens and A. Windemuth, “The predictive power of haplotypes in clinical response,” Pharmacogenomics. February 2000; 1(1): 15-26).

[0465] SNPs are identified by a variety of art-accepted methods (P. Bean, “The promising voyage of SNP target discovery,” Am. Clin. Lab. October-November 2001; 20(9):18-20; K. M. Weiss, “In search of human variation,” Genome Res. July 1998; 8(7):691-697; M. M. She, “Enabling large-scale pharmacogenetic studies by high-throughput mutation detection and genotyping technologies,” Clin. Chem. February 2001; 47(2): 164-172).

[0466] For example, SNPs are identified by sequencing DNA fragments that show polymorphism by gel-based methods such as restriction fragment length polymorphism (RFLP) and denaturing gradient gel electrophoresis (DGGE). SNPs can also be discovered by direct sequencing of DNA samples pooled from different individuals or by comparing sequences from different DNA samples. With the accumulation of sequence data in public and private databases, one can also discover SNPs by comparing sequences using computer programs (Z. Gu, L. Hillier and P. Y. Kwok, “Single nucleotide polymorphism hunting in cyberspace,” Hum. Mutat. 1998; 12(4):221-225). SNPs can be verified by a variety of methods including direct sequencing and high throughput microarrays (P. Y. Kwok, “Methods for genotyping single nucleotide polymorphisms,” Annu. Rev. Genomics Hum. Genet. 2001; 2:235-258; M. Kokoris, K. Dix, K. Moynihan, J. Mathis, B. Erwin, P. Grass, B. Hines and A. Duesterhoeft, “High-throughput SNP genotyping with the Masscode system,” Mol. Diagn. 2000 Dec; 5(4):329-340).

[0467] As disclosed herein SNPs are identified by directly sequencing cDNA clones and by comparing our sequences with public and proprietary sequences. By sequencing cDNA clones, SNPs are identified. By comparing these sequences with high quality proprietary or public sequences (e.g, NCBI/GenBank, accesible at World Wide Web URL www.ncbi.nlm.nih.gov), SNPs are identified. SNPs are identified by aligning variant sequences with NCBI genes and ESTs. Typically, only ESTs with over 97% identity are considered; differences within 50 base pairs of the ends are not considered. Only SNPs that occur twice from two independent sequences are included.

Example 6 Production of Recombinant 121P1F1 in Prokaryotic Systems

[0468] To express recombinant 121P1F1 in prokaryotic cells, the full or partial length 121P1F1 cDNA sequences can be cloned into any one of a variety of expression vectors known in the art. One or more of the following regions of 121P1F1 are expressed in these constructs: amino acids 1 to 205 of 121P1F1; amino acids 1-126 of splice variant 1a; amino acids 1-119 of splice variant 1b; amino acids 1-122 of splice variant 2; amino acids 1-190 of splice variant 3; amino acids 1-190 of splice variant 4, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 121P1F1, splice variants, or analogs thereof.

[0469] A. In Vitro Transcription and Translation Constructs:

[0470] pCRII: To generate 121P1F1 sense and anti-sense RNA probes for RNA in situ investigations, pCRII constructs (Invitrogen, Carlsbad Calif.) are generated encoding either all or fragments of the 121P1F1 cDNA. The pCRII vector has Sp6 and T7 promoters flanking the insert to drive the transcription of 121P1F1 RNA for use as probes in RNA in situ hybridization experiments. These probes are used to analyze the cell and tissue expression of 121P1F1 at the RNA level. Transcribed 121P1F1 RNA representing the cDNA amino acid coding region of the 121P1F1 gene is used in in vitro translation systems such as the TnT™ Coupled Reticulolysate Sytem (Promega, Corp., Madison, Wis.) to synthesize 121P1F1 protein.

[0471] B. Bacterial Constructs:

[0472] pGEX Constructs: To generate recombinant 121P1F1 proteins in bacteria that are fused to the Glutathione S-transferase (GST) protein, all or parts of the 121P1F 1 cDNA protein coding sequence are fused to the GST gene by cloning into pGEX-6P-1 or any other GST-fusion vector of the pGEX family (Amersham Pharmacia Biotech, Piscataway, N.J.). These constructs allow controlled expression of recombinant 121P1F1 protein sequences with GST fused at the amino-terminus and a six histidine epitope (6× His) at the carboxyl-terminus. The GST and 6× His tags permit purification of the recombinant fusion protein from induced bacteria with the appropriate affinity matrix and allow recognition of the fusion protein with anti-GST and anti-His antibodies. The 6× His tag is generated by adding 6 histidine codons to the cloning primer at the 3′ end, e.g., of the open reading frame (ORF). A proteolytic cleavage site, such as the PreScission™ recognition site in pGEX-6P-1, can be employed such that it permits cleavage of the GST tag from 121P1F1-related protein. The ampicillin resistance gene and pBR322 origin permits selection and maintenance of the pGEX plasmids in E. coli. In one embodiment, amino acids 1-114 of 121P1F1 is cloned into the pGEX-6P-1 vector, expressed in bacteria, purified, and a 121P1F1 cleavage product generated utilizing PreScission protease.

[0473] pMAL Constructs: To generate, in bacteria, recombinant 121P1F1 proteins that are fused to maltose-binding protein (MBP), all or parts of the 121P1F1 cDNA protein coding sequence are fused to the MBP gene by cloning into the pMAL-c2X and pMAL-p2X vectors (New England Biolabs, Beverly, Mass.). These constructs allow controlled expression of recombinant 121P1F1 protein sequences with MBP fused at the amino-terminus and a 6× His epitope tag at the carboxyl-terminus. The MBP and 6× His tags permit purification of the recombinant protein from induced bacteria with the appropriate affinity matrix and allow recognition of the fusion protein with anti-MBP and anti-His antibodies. The 6× His epitope tag is generated by adding 6 histidine codons to the 3′ cloning primer. A Factor Xa recognition site permits cleavage of the pMAL tag from 121P1F1. The pMAL-c2X and pMAL-p2X vectors are optimized to express the recombinant protein in the cytoplasm or periplasm respectively. Periplasm expression enhances folding of proteins with disulfide bonds.

[0474] pT Constructs: To express 121P1F1 in bacterial cells, all or parts of the 121P1F1 cDNA protein coding sequence are cloned into the pET family of vectors (Novagen, Madison, Wis.). These vectors allow tightly controlled expression of recombinant 121P1F1 protein in bacteria with and without fusion to proteins that enhance solubility, such as NusA and thioredoxin (Trx), and epitope tags, such as 6×His and S-Tag™ that aid purification and detection of the recombinant protein. For example, constructs are made utilizing pET NusA fusion system 43.1 such that regions of the 121P1F1 protein are expressed as amino-terminal fusions to NusA.

[0475] C. Yeast Constructs:

[0476] ESC Constructs: To express 121P1F1 in the yeast species Saccharomyces cerevisiae for generation of recombinant protein and functional studies, all or parts of the 121P1F 1 cDNA protein coding sequence are cloned into the pESC family of vectors each of which contain 1 of 4 selectable markers, HIS3, TRP1, LEU2, and URA3 (Stratagene, La Jolla, Calif.). These vectors allow controlled expression from the same plasmid of up to 2 different genes or cloned sequences containing either Flag™ or Myc epitope tags in the same yeast cell. This system is useful to confirm protein-protein interactions of 121P1F1. In addition, expression in yeast yields similar post-translational modifications, such as glycosylations and phosphorylations, that are found when expressed in eukaryotic cells.

[0477] pESP Constructs: To express 121P1F1 in the yeast species Saccharomyces pombe, all or parts of the 121P1F1 cDNA protein coding sequence are cloned into the pESP family of vectors. These vectors allow controlled high level of expression of a 121P1F1 protein sequence that is fused at either the amino terminus or at the carboxyl terminus to GST which aids purification of the recombinant protein. A Flag™ epitope tag allows detection of the recombinant protein with anti-Flag™ antibody.

Example 7 Production of Recombinant 121P1F1 in Eukaryotic Systems

[0478] A. Mammalian Constructs:

[0479] One or more of the following regions of 121P1F1 are expressed in these constructs: amino acids 1 to 205 of 121P1F1; amino acids 1-126 of splice variant 1a; amino acids 1-119 of splice variant 1b; amino acids 1-122 of splice variant 2; amino acids 1-190 of splice variant 3; amino acids 1-190 of splice variant 4, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 121P1F1, splice variants, or analogs thereof. In certain embodiments a region of 121P1F1 is expressed that encodes an amino acid not shared amongst at least two variants.

[0480] The constructs can be transfected into any one of a wide variety of mammalian cells such as 293T cells. Transfected 293T cell lysates can be probed with the anti-121P1F1 polyclonal serum, described herein.

[0481] pcDNA4/HisMax Constructs: To express 121P1F1 in mammalian cells, a 121P1F1 ORF, or portions thereof, of 121P1F1 are cloned into pcDNA4/HisMax Version A (Invitrogen, Carlsbad, Calif.). Protein expression is driven from the cytomegalovirus (CMV) promoter and the SP16 translational enhancer. The recombinant protein has Xpress™ and six histidine (6×His) epitopes fused to the amino-terminus. The pcDNA4/HisMax vector also contains the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability along with the SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen. The Zeocin resistance gene allows for selection of mammalian cells expressing the protein and the ampicillin resistance gene and ColE1 origin permits selection and maintenance of the plasmid in E. coli.

[0482] pcDNA3.1/MycHis Constructs: To express 121P1F1 in mammalian cells, a 121P1F1 ORF, or portions thereof, of 121P1F1 with a consensus Kozak translation initiation site is cloned into pcDNA3.1/MycHis Version A (Invitrogen, Carlsbad, Calif.). Protein expression is driven from the cytomegalevirus (CMV) promoter. The recombinant proteins have the myc epitope and 6× His epitope fused to the; carboxyl-terminus. The pcDNA3.1/MycHis vector also contains the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability, along with the SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen. The Neomycin resistance gene can be used, as it allows for selection of mammalian cells expressing the protein and the ampicillin resistance gene and ColE1 origin permits selection and maintenance of the plasmid in E. coli. FIG. 14 shows expression of 121P1F1 pcDNA3.1/mychis in transiently infected 293T cells.

[0483] pcDNA3.1/CT-GFP-TOPO Construct: To express 121P1F1 in mammalian cells and to allow detection of the recombinant proteins using fluorescence, a 121P1F1 ORF, or portions thereof, with a consensus Kozak translation initiation site are cloned into pcDNA3.1/CT-GFP-TOPO (Invitrogen, Calif.). Protein expression is driven from the cytomegalovirus (CMV) promoter. The recombinant proteins have the Green Fluorescent Protein (GFP) fused to the carboxyl-terminus facilitating non-invasive, in vivo detection and cell biology studies. The pcDNA3.1CT-GFP-TOPO vector also contains the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability along with the SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen. The Neomycin resistance gene allows for selection of mammalian cells that express the protein, and the ampicillin resistance gene and ColE1 origin permits selection and maintenance of the plasmid in E. coli. Additional constructs with an amino-terminal GFP fusion are made in pcDNA3.1NT-GFP-TOPO spanning the entire length of a 121P1F1 protein.

[0484] PAPtag: A 121P1F1 ORF, or portions thereof, is cloned into pAPtag-5 (GenHunter Corp. Nashville, Tenn.). This construct generates an alkaline phosphatase fusion at the carboxyl-terminus of a 121P1F1 protein while fusing the IgGK signal sequence to the amino-terminus. Constructs are also generated in which alkaline phosphatase with an amino-terminal IgGK signal sequence is fused to the amino-terminus of a 121P1F1 protein. The resulting recombinant 121P1F1 proteins are optimized for secretion into the media of transfected mammalian cells and can be used to identify proteins such as ligands or receptors that interact with 121P1F1 proteins. Protein expression is driven from the CMV promoter and the recombinant proteins also contain myc and 6× His epitopes fused at the carboxyl-terminus that facilitates detection and purification. The Zeocin resistance gene present in the vector allows for selection of mammalian cells expressing the recombinant protein and the ampicillin resistance gene permits selection of the plasmid in E. coli.

[0485] ptag5. A 121P1F1 ORF, or portions thereof, is cloned into pTag-5. This vector is similar to pAPtag but without the alkaline phosphatase fusion. This construct generates 121P1F1 protein with an amino-terminal IgGK signal sequence and myc and 6× His epitope tags at the carboxyl-terminus that facilitate detection and affinity purification. The resulting recombinant 121P1F1 protein is optimized for secretion into the media of transfected mammalian cells, and is used as immunogen or ligand to identify proteins such as ligands or receptors that interact with the 121P1F1 proteins. Protein expression is driven from the CMV promoter. The Zeocin resistance gene present in the vector allows for selection of mammalian cells expressing the protein, and the ampicillin resistance gene permits selection of the plasmid in E. coli.

[0486] PsecFc: A 121P1F1 ORF, or portions thereof, is also cloned into psecFc. The psecFc vector was assembled by cloning the human immunoglobulin GI (IgG) Fc (hinge, CH2, CH3 regions) into pSecTag2 (Invitrogen, Calif.). This construct generates an IgG1 Fc fusion at the carboxyl-terminus of the 121P1F1 proteins, while fusing the IgGK signal sequence to N-terminus. 121P1F1 fusions utilizing the murine IgG1 Fc region are also used. The resulting recombinant 121P1F1 proteins are optimized for secretion into the media of transfected mammalian cells, and can be used as immunogens or to identify proteins such as ligands or receptors that interact with 121P1F1 protein. Protein expression is driven from the CMV promoter. The hygromycin resistance gene present in the vector allows for selection of mammalian cells that express the recombinant protein, and the ampicillin resistance gene permits selection of the plasmid in E. coli.

[0487] pSRα Constructs: To generate mammalian cell lines that express 121P1F1 constitutively, 121P1F1 ORF, or portions thereof, of 121P1F1 are cloned into pSRα constructs. Amphotropic and ecotropic retroviruses are generated by transfection of pSRα constructs into the 293T-10A1 packaging line or co-transfection of pSRα and a helper plasmid (containing deleted packaging sequences) into the 293 cells, respectively. The retrovirus is used to infect a variety of mammalian cell lines, resulting in the integration of the cloned gene, 121P1F1, into the host cell-lines. Protein expression is driven from a long terminal repeat (LTR). The Neomycin resistance gene present in the vector allows for selection of mammalian cells that express the protein, and the ampicillin resistance gene and ColE1 origin permit selection and maintenance of the plasmid in E. coli. The retroviral vectors can thereafter be used for infection and generation of various cell lines using, for example, PC3, NIH 3T3, TsuPr1, 293 or rat-1 cells.

[0488] Additional pSRα constructs are made that fuse an epitope tag such as the FLAG™ tag to the carboxyl-terminus of 121P1F1 sequences to allow detection using anti-Flag antibodies. For example, the FLAG™ sequence 5′ gat tac aag gat gac gac gat aag 3′ (SEQ ID NO: ______) is added to cloning primer at the 3′ end of the ORF. Additional pSRα constructs are made to produce both amino-terminal and carboxyl-terminal GFP and myc/6× His fusion proteins of the full-length 121P1F1 proteins.

[0489] Additional Viral Vectors: Additional constructs are made for viral-mediated delivery and expression of 121P1F1. High virus titer leading to high level expression of 121P1F1 is achieved in viral delivery systems such as adenoviral vectors and herpes amplicon vectors. A 121P1F1 coding sequences or fragments thereof are amplified by PCR and subcloned into the AdEasy shuttle vector (Stratagene). Recombination and virus packaging are performed according to the manufacturer's instructions to generate adenoviral vectors. Alternatively, 121P1F1 coding sequences or fragments thereof are cloned into the HSV-1 vector (Imgenex) to generate herpes viral vectors. The viral vectors are thereafter used for infection of various cell lines such as PC3, NIH 3T3, 293 or rat-1 cells.

[0490] Regulated Expression Systems: To control expression of 121P1F1 in mammalian cells, coding sequences of 121P1F1, or portions thereof, are cloned into regulated mammalian expression systems such as the T-Rex System (Invitrogen), the GeneSwitch System (Invitrogen) and the tightly-regulated Ecdysone System (Sratagene). These systems allow the study of the temporal and concentration dependent effects of recombinant 121P1F1. These vectors are thereafter used to control expression of 121P1F1 in various cell lines such as PC3, NIH 3T3, 293 or rat-1 cells.

[0491] B. Baculovirus Expression Systems

[0492] To generate recombinant 121P1F1 proteins in a baculovirus expression system, 121P1F1 ORF, or portions thereof, are cloned into the baculovirus transfer vector pBlueBac 4.5 (Invitrogen), which provides a His-tag at the N-terminus. Specifically, pBlueBac-121P1F1 is co-transfected with helper plasmid pBac-N-Blue (Invitrogen) into SF9 (Spodoptera frugiperda) insect cells to generate recombinant baculovirus (see Invitrogen instruction manual for details). Baculovirus is then collected from cell supernatant and purified by plaque assay.

[0493] Recombinant 121P1F1 protein is then generated by infection of HighFive insect cells (Invitrogen) with purified baculovirus. Recombinant 121P1F1 protein can be detected using anti-121P1F1 or anti-His-tag antibody. 121P1F1 protein can be purified and used in various cell-based assays or as immunogen to generate polyclonal and monoclonal antibodies specific for 121P1F1.

Example 8 Antigenicity Profiles and Secondary Structure

[0494]FIG. 5A, FIG. 6A, FIG. 7A, FIG. 8A, and FIG. 9A depict graphically five amino acid profiles of the 121P1F1 amino acid sequence; FIG. 5B, FIG. 6B, FIG. 7B, FIG. 8B, and FIG. 9B depict graphically five amino acid profiles of the 121P1F1 variant 1A amino acid sequence. Each assessment is available by accessing the ProtScale website (URL www.expasy.ch/cgi-bin/protscale.pl) on the ExPasy molecular biology server.

[0495] These profiles: FIG. 5, Hydrophilicity, (Hopp T. P., Woods K. R., 1981. Proc. Natl. Acad. Sci. U.S.A. 78:3824-3828); FIG. 6, Hydropathicity, (Kyte J., Doolittle R. F., 1982. J. Mol. Biol. 157:105-132); FIG. 7, Percentage Accessible Residues (Janin J., 1979 Nature 277:491-492); FIG. 8, Average Flexibility, (Bhaskaran R., and Ponnuswamy P. K., 1988. Int. J. Pept. Protein Res. 32:242-255); FIG. 9, Beta-turn (Deleage, G., Roux B. 1987 Protein Engineering 1:289-294); and optionally others available in the art, such as on the ProtScale website, were used to identify antigenic regions of the 121P1F1 protein and variant IA. Each of the above amino acid profiles of 121P1F1 were generated using the following ProtScale parameters for analysis: 1) A window size of 9; 2) 100% weight of the window edges compared to the window center; and 3) amino acid profile values normalized to lie between 0 and 1.

[0496] Hydrophilicity (FIG. 5), Hydropathicity (FIG. 6) and Percentage Accessible Residues (FIG. 7) profiles were used to determine stretches of hydrophilic amino acids (i.e., values greater than 0.5 on the Hydrophilicity and Percentage Accessible Residues profile, and values less than 0.5 on the Hydropathicity profile). Such regions are likely to be exposed to the aqueous environment, be present on the surface of the protein, and thus available for immune recognition, such as by antibodies.

[0497] Average Flexibility (FIG. 8) and Beta-turn (FIG. 9) profiles determine stretches of amino acids (i.e., values greater than 0.5 on the Beta-turn profile and the Average Flexibility profile) that are not constrained in secondary structures such as beta sheets and alpha helices. Such regions are also more likely to be exposed on the protein and thus accessible to immune recognition, such as by antibodies.

[0498] Antigenic sequences of the full length 121P1F1 protein indicated, e.g., by the profiles set forth in FIG. 5A, FIG. 6A, FIG. 7A, FIG. 8A, and/or FIG. 9A are used to prepare immunogens, either peptides or nucleic acids that encode them, to generate therapeutic and diagnostic anti-121P1F1 antibodies. Antigenic sequences of the 121P1F1 variant 1A protein indicated, e.g., by the profiles set forth in FIG. 5B, FIG. 6B, FIG. 7B, FIG. 8B, and/or FIG. 9B are used to prepare immunogens, either peptides or nucleic acids that encode them, to generate therapeutic and diagnostic anti-121P1F1-variant 1A antibodies. The immunogen can be any 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50 or more than 50 contiguous amino acids, or the corresponding nucleic acids that encode them, from the 121P1F1 protein or from variants 1a, 1b, 2, 3, or 4 (see FIGS. 2 and 3). In particular, peptide immunogens of the invention can comprise, a peptide region of at least 5 amino acids of FIG. 2 in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Hydrophilicity profile of FIG. 5; a peptide region of at least 5 amino acids of FIG. 2 in any whole number increment up to 205 that includes an amino acid position having a value less than 0.5 in the Hydropathicity profile of FIG. 6; a peptide region of at least 5 amino acids of FIG. 2 in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Percent Accessible Residues profile of FIG. 7; a peptide region of at least 5 amino acids of FIG. 2 in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Average Flexibility profile on FIG. 8; and, a peptide region of at least 5 amino acids of FIG. 2 in any whole number increment up to 205 that includes an amino acid position having a value greater than 0.5 in the Beta-turn profile of FIG. 9. Peptide immunogens of the invention can also comprise nucleic acids that encode any of the forgoing. In addition, peptide immunogens can comprise amino acids of variant 1a, that contain. characteristics of the above mentioned parameters set forth in FIG. 5B, FIG. 6B, FIG. 7B, FIG. 8B, or FIG. 9B.

[0499] All immunogens of the invention, peptide or nucleic acid, can be embodied in human unit dose form, or comprised by a composition that includes a pharmaceutical excipient compatible with human physiology.

[0500] The secondary structure of 121P1F1, namely the predicted presence and location of alpha helices, extended strands, and random coils, is predicted from the primary amino acid sequence using the HNN—Hierarchical Neural Network method (Guermeur, 1997, Web URL pbil.ibcp.fr/cgi-bin/npsa_automat.p1?page=npsa_nn.html), accessed from the ExPasy molecular biology server (Web URL www.expasy.ch/tools/). The analysis indicates that 121P1F1 is composed 61.95% alpha helix, 1.95% extended strand, and 36.10% random coil (FIG. 16A). The secondary structure of variant 1 a is presented in FIG. 115B.

[0501] Analysis of 121P1F1 using a variety of transmembrane prediction algorithms accessed from the ExPasy molecular biology server (Web URL www.expasy.ch/tools/) did not predict the presence of such domains, suggesting that 121P1F1 and the variants are soluble proteins.

Example 9 Generation of 121P1F1 Polyclonal Antibodies

[0502] Polyclonal antibodies can be raised in a mammal, for example, by one or more injections of an immunizing, agent and, if desired, an adjuvant. Typically, the immunizing agent and/or adjuvant will be injected in the mammal by multiple subcutaneous or intraperitoneal injections. In addition to immunizing with the full length 121P1F1 protein, computer algorithms are employed in design of immunogens that, based on amino acid sequence analysis, contain characteristics of being antigenic and available for recognition by the immune system of the immunized host (see the Example entitled “Antigenicity Profiles”). Such regions would be predicted to be hydrophilic, flexible, in beta-tum conformations, and be exposed on the surface of the protein (see, e.g., FIG. 5A, FIG. 6A, FIG. 7A, FIG. 8A, or FIG. 9A for amino acid profiles that indicate such regions of 121P1F1; and FIG. 5B, FIG. 6B, FIG. 7B, FIG. 8B, or FIG. 9B for amino acid profiles that indicate such regions of 121P1F1 variant la).

[0503] For example, 121P1F1 recombinant bacterial fusion proteins or peptides containing hydrophilic, flexible, be α-turn regions of 121P1F1 or of the variants are used as antigens to generate polyclonal antibodies in New Zealand White rabbits. For example, such regions include, but are not limited to, amino acids 1-50 and amino acids 90-160 of 121P1F1. In addition, immunogens are designed to encode regions that are novel to particular variants of 121P1F1, such as amino acids 93-126 of variant 1a, amino acids 1-6 of variant 1b, and amino acids 117-122 of variant 2. Antibodies to these regions are useful to distinguish between 121P1F1 and its splice variants. It is useful to conjugate the immunizing agent to a protein known to be immunogenic in the mammal being immunized. Examples of such immunogenic proteins include, but are not limited to, keyhole limpet hemocyanin (KLH), serum albumin, bovine thyroglobulin, and soybean trypsin inhibitor. In one embodiment, a peptide encoding amino acids 1-25 of 121P1F1 is conjugated to KLH and used to immunize the rabbit. Alternatively the immunizing agent can include all or portions of the 121P1F1 protein, analogs or fusion proteins thereof. For example, the 121P1F1 amino acid sequence can be fused using recombinant DNA techniques to any one of a variety of fusion protein partners that are well known in the art, such as glutathione-S-transferase (GST) and HIS tagged fusion proteins. Such fusion proteins are purified from induced bacteria using the appropriate affinity matrix.

[0504] In one embodiment, a GST-fusion protein encoding amino acids 1-114 of 121P1F1 coding sequence is produced, purified, and a proteolytic cleavage product in which GST sequences are removed is used as immunogen. Other recombinant bacterial fusion proteins that can be employed include maltose binding protein, LacZ, thioredoxin, NusA, or an immunoglobulin constant region (see the section entitled “Production of 121P1F1 in Prokaryotic Systems” and Current Protocols In Molecular Biology, Volume 2, Unit 16, Frederick M. Ausubul et al. eds., 1995; Linsley, P.S., Brady, W., Umes, M., Grosmaire, L., Damle, N., and Ledbetter, L.(1991) J.Exp. Med. 174, 561-566).

[0505] In addition to bacterial derived fusion proteins, mammalian expressed protein antigens are also used. These antigens are expressed from mammalian expression vectors such as the Tag5 and Fc-fusion vectors (see the section entitled “Production of Recombinant 121P1F1 in Eukaryotic Systems”), and retain post-translational modifications such as glycosylations found in native protein. In one embodiment, the entire 121P1F1 coding sequence is cloned into the Tag5 mammalian secretion vector. The recombinant protein is purified by metal chelate chromatography from tissue culture supernatants of 293T cells stably expressing the recombinant vector. The purified Tag5 121P1F1 protein is then used as immunogen.

[0506] During the immunization protocol, it is useful to mix or emulsify the antigen in adjuvants that enhance the immune response of the host animal. Examples of adjuvants include, but are not limited to, complete Freund's adjuvant (CFA) and MPL-TDM adjuvant (monophosphoryl Lipid A, synthetic trehalose dicorynomycolate).

[0507] In a typical protocol, rabbits are initially immunized subcutaneously with up to 200 μg, typically 100-200 μg, of fusion protein or peptide conjugated to KLH mixed in complete Freund's adjuvant (CFA). Rabbits are then injected subcutaneously every two weeks with up to 200 μg, typically 100-200 μg, of the immunogen in incomplete Freund's adjuvant (IFA). Test bleeds are taken approximately 7-10 days following each immunization and used to monitor the titer of the antiserum by ELISA.

[0508] Tile reactivity of serum from immunized animals is tested by various immunoassays, such as ELISA, Western blot, immunofluorescence microscopy, and flow cytometry. The reactivity of the anti-GST-cleavage product serum was tested by Western blot using various amounts of immunogen; see FIG. 12, which shows strong and specific reactivity of the serum to the cleavage antigen. Antiserum is then purified by various affinity chromatography techniques.

[0509] The anti-serum from the GST-fusion cleavage immunogen is affinity purified by passage over a column composed of the GST-cleavage antigen covalently coupled to Affigel matrix (BioRad, Hercules, Calif.). The serum is then further purified by protein G affinity chromatography to isolate the IgG fraction. Serum from rabbits immunized with whole fusion proteins, such as GST and MBP fusion proteins, are purified by depletion of antibodies reactive to the fusion partner sequence by passage over an affinity column containing the fusion partner either alone or in the context of an irrelevant fusion protein. Sera from other His-tagged antigens and peptide immunized rabbits as well as fusion partner depleted sera are affinity purified by passage over a column matrix composed of the original protein immunogen or free peptide.

[0510] Both crude and affinity purified polyclonal antibodies are further tested by various immunoassays against both recombinant cells and cells and tissues that endogenously express 121P1F1. To generate recombinant 121P1F1 cells, the full-length 12P1F1 cDNA is cloned into pcDNA 3.1 Myc-His expression vector (Invitrogen, see the Example entitled “Production of Recombinant 121P1F1 in Eukaryotic Systems”). After transfection of the construct into 293T cells, cell lysates were probed with the anti-121P1F1 polyclonal antibody (FIG. 13) and with anti-His antibody (Santa Cruz Biotechnologies, Santa Cruz, Calif.) (FIG. 14) demonstrating specific reactivity to denatured 121P1F1 protein using the Western blot technique. The polyclonal antibody was also used to test a panel of tumor cell lines by Western analysis, for which the results are also shown in FIG. 13. The polyclonal antibody shows strong reactivity to MYC-HIS tagged 121P1F1 in transfected 293T cells and also to several proteins in the tumor cell lines, indicating reactivity to endogenous 121P1F1 and to variant molecules of different molecular weights. In addition, immunoprecipitation, fluorescent microscopy, immunohistochemistry, and flow cytometric techniques on recombinant cells and patient tissues samples are used to characterize 121P1F1 protein expression using the polyclonal antibody.

Example 10 Generation of 121P1F1 Monoclonal Antibodies (mAbs)

[0511] In one embodiment, therapeutic mAbs to 121P1F1 comprise those that react with epitopes of the protein that would disrupt or modulate the biological function of 121P1F1, for example those that would disrupt its interaction with ligands, proteins, or substrates that mediate its biological activity. Immunogens for generation of such mAbs include those designed to encode or contain the entire 121P1F1 protein or its variants or regions of the 121P1F1 protein or its variants predicted to be antigenic from computer analysis of the amino acid sequence (see, e.g., FIG. 5, FIG. 6, FIG. 7, FIG. 8, or FIG. 9, and the Example entitled “Antigenicity Profiles”). Immunogens include peptides, recombinant bacterial proteins, and mammalian expressed Tag 5 proteins and human and murine IgG FC fusion proteins. In addition, cells expressing high levels of 121P1F1, such as 293T-121P1F1 or 300.19-121P1F1 murine Pre-B cells, are used to immunize mice.

[0512] To generate mAbs to 121P1F1, mice are first immunized intraperitoneally (IP) with, typically, 10-50 μg of protein immunogen or 10⁷ 121P1F1-expressing cells mixed in complete Freund's adjuvant. Mice are then subsequently immunized IP every 24 weeks with, typically, 10-50 μg of protein immunogen or 10⁷ cells mixed in incomplete Freund's adjuvant. Alternatively, MPL-TDM adjuvant is used in immunizations. In addition to the above protein and cell-based immunization strategies, a DNA-based immunization protocol is employed in which a mammalian expression vector encoding 121P1F1 sequence is used to immunize mice by direct injection of the plasmid DNA. For example, the entire coding sequence of 121P1F1, amino acids 1-205, is cloned into the Tag5 mammalian secretion vector and the recombinant vector is used as immunogen. In another example the same amino acids are cloned into an Fc-fusion secretion vector in which the 121P1F1 sequence is fused at the amino-terminus to an IgK leader sequence and at the carboxyl-terminus to the coding sequence of the human or murine IgG Fc region. This recombinant vector is then used as immunogen. The plasmid immunization protocols are used in combination with purified proteins expressed from the same vector and with cells expressing 121P1F1. In another embodiment the GST-fusion cleavage protein described in Example 8 is used as immunogen.

[0513] During the immunization protocol, test bleeds are taken 7-10 days following an injection to monitor titer and specificity of the immune response. Once appropriate reactivity and specificity is obtained as determined by ELISA, Western blotting, immunoprecipitation, fluorescence microscopy, and flow cytometric analyses, fusion and hybridoma generation is then carried out with established procedures well known in the art (see, e.g., Antibodies: A Laboratory Manual, CSH Press, Eds., Harlow, and Lane (1988)).

[0514] In one embodiment, monoclonal antibodies are derived that distinguish variant 1a from 121P1F1 and the other variants. For example, a Tag5 protein encoding amino acids 93-126 of variant 1a is produced and purified. from the supernatants of 293T cells transfected with the cognate Tag5 cDNA vector. Balb C mice are initially immunized intraperitoneally with 25 μg of the Tag5-variant 1 a protein mixed in complete Freund's adjuvant. Mice are subsequently immunized every two weeks with 25 μg of the antigen mixed in incomplete Freund's adjuvant for a total of three immunizations. ELISA using the Tag5 antigen determines the titer of serum from immunized mice. Reactivity and specificity of serum to the full length variant 1a protein is monitored by Western blotting, immunoprecipitation and flow cytometry using 293T cells transfected with an expression vector encoding the variant 1a cDNA (see e.g., the Example entitled “Production of Recombinant 121P1F1 in Eukaryotic Systems”). Other recombinant variant 1a-expressing cells or cells endogenously expressing variant 1a are also used. Specificity is also determined by lack of reactivity to cells expressing 121P1F1 and the other variants. Mice showing the strongest reactivity to variant 1a are rested and given a final injection of Tag5 antigen in PBS and then sacrificed four days later. The spleens of the sacrificed mice are harvested and fused to SPO/2 myeloma cells using standard procedures (Harlow and Lane, 1988). Supernatants from HAT selected growth wells are screened by ELISA, Western blot, immunoprecipitation, fluorescent microscopy, and flow cytometry to identify 121P1F1 specific antibody-producing clones. Monoclonal antibodies are also raised that distinguish variant 1b and variant 2 from each other, from variants 3 and 4 and from 121P1F1. This is accomplished through immunization with antigens, such as KLH-coupled peptides, that encode amino acids specific to variant 1b (amino acids 1-6) and variant 2 (amino acids 118-122).

[0515] The binding affinity of a 121P1F1 monoclonal antibody is determined using standard technologies. Affinity measurements quantify the strength of antibody to epitope binding and are used to help define which 121P1F1 monoclonal antibodies preferred for diagnostic or therapeutic use, as appreciated by one of skill in the art. The BIAcore system (Uppsala, Sweden) is a preferred method for determining binding affinity. The BIAcore system uses surface plasmon resonance (SPR, Welford K. 1991, Opt. truant. Elect. 23:1; Morton and Myszka, 1998, Methods in Enzymology 295: 268) to monitor biomolecular interactions in real time. BIAcore analysis conveniently generates association rate constants, dissociation rate constants, equilibrium dissociation constants, and affinity constants.

Example 11 HLA Class I and Class II Binding Assays

[0516] HLA class I and class II binding assays using purified HLA molecules are performed in accordance with disclosed protocols (e.g., PCT publications WO 94/20127 and WO 94/03205; Sidney et al., Current Protocols in Immunology 18.3.1 (1998); Sidney, et al., J. Immunol. 154:247 (1995); Sette, et al., Mol. Immunol. 31:813 (1994)). Briefly, purified MHC molecules (5 to 500 nM) are incubated with various unlabeled peptide inhibitors and 1-10 nM ¹²⁵I-radiolabeled probe peptides as described. Following incubation, MHC-peptide complexes are separated from free peptide by gel filtration and the fraction of peptide bound is determined. Typically, in preliminary experiments, each MHC preparation is titered in the presence of fixed amounts of radiolabeled peptides to determine the concentration of HLA molecules necessary to bind 10-20% of the total radioactivity. All subsequent inhibition and direct binding assays are performed using these HLA concentrations.

[0517] Since under these conditions [label]<[HLA] and IC₅₀≧[HLA], the measured IC₅₀ values are reasonable approximations of the true K_(D) values. Peptide inhibitors are typically tested at concentrations ranging from 120 μg/ml to 1.2 ng/ml, and are tested in two to four completely independent experiments. To allow comparison of the data obtained in different experiments, a relative binding figure is calculated for each peptide by dividing the IC₅₀ of a positive control for inhibition by the IC₅₀ for each tested peptide (typically unlabeled versions of the radiolabeled probe peptide). For database purposes, and inter-experiment comparisons, relative binding values are compiled. These values can subsequently be converted back into IC₅₀ nM values by dividing the IC₅₀ nM of the positive controls for inhibition by the relative binding of the peptide of interest. This method of data compilation is accurate and consistent for comparing peptides that have been tested on different days, or with different lots of purified MHC.

[0518] Binding assays as outlined above may be used to analyze HLA supermotif and/or HLA motif-bearing peptides.

Example 12 Identification of HLA Supermotif- and Motif-Bearing CTL Candidate Epitopes

[0519] HLA vaccine compositions of the invention can include multiple epitopes. The multiple epitopes can comprise multiple HLA supermotifs or motifs to achieve broad population coverage. This example illustrates the identification and confirmation of supermotif- and motif-bearing epitopes for the inclusion in such a vaccine composition. Calculation of population coverage is performed using the strategy described below.

[0520] Computer Searches and Algorithms for Identification of Supermotif and/or Motif-Bearing Epitopes

[0521] The searches performed to identify the motif-bearing peptide sequences in the Example entitled “Antigenicity Profiles” and Tables V-XVIII, XXVI, and XXVII employ the protein sequence data from the gene product of 121P1F1 set forth in FIGS. 2 and 3.

[0522] Computer searches for epitopes bearing HLA Class I or Class II supermotifs or motifs are performed as follows. All translated 121P1F1 protein sequences are analyzed using a text string search software program to identify potential peptide sequences containing appropriate HLA binding motifs; such programs are readily produced in accordance with information in the art in view of known motif/supermotif disclosures. Furthermore, such calculations can be made mentally.

[0523] Identified A2-, A3-, and DR-supermotif sequences are scored using polynomial algorithms to predict their capacity to bind to specific HLA-Class I or Class II molecules. These polynomial algorithms account for the impact of different amino acids at different positions, and are essentially based on the premise that the overall affinity (or AG) of peptide-HLA molecule interactions can be approximated as a linear polynomial function of the type:

“ΔG”=a _(1i) ×a _(2i) ×a _(3i) . . . ×a _(ni)

[0524] where a_(ji) is a coefficient which represents the effect of the presence of a given amino acid (i) at a given position (i) along the sequence of a peptide of n amino acids. The crucial assumption of this method is that the effects at each position are essentially independent of each other (i.e., independent binding of individual side-chains). When residue j occurs at position i in the peptide, it is assumed to contribute a constant amount j_(i) to the free energy of binding of the peptide irrespective of the sequence of the rest of the peptide.

[0525] The method of derivation of specific algorithm coefficients has been described in Gulukota et al., J. Mol. Biol. 267:1258-126, 1997; (see also Sidney et al., Human Immunol. 45:79-93, 1996; and Southwood et al., J. Immunol. 160:3363-3373, 1998). Briefly, for all i positions, anchor and non-anchor alike, the geometric mean of the average relative binding (ARB) of all peptides carrying j is calculated relative to the remainder of the group, and used as the estimate of j_(i). For Class II peptides, if multiple alignments are possible, only the highest scoring alignment is utilized, following an iterative procedure. To calculate an algorithm score of a given peptide in a test set, the ARB values corresponding to the sequence of the peptide are multiplied. If this product exceeds a chosen threshold, the peptide is predicted to bind. Appropriate thresholds are chosen as a function of the degree of stringency of prediction desired.

[0526] Selection of HLA-A2 Supertype Cross-Reactive Peptides

[0527] Protein sequences from 121P1F1 are scanned utilizing motif identification software, to identify 8-, 9-10- and 11-mer sequences containing the HLA-A2-supermotif main anchor specificity. Typically, these sequences are then scored using the protocol described above and the peptides corresponding to the positive-scoring sequences are synthesized and tested for their capacity to bind purified HLA-A*0201 molecules in vitro (HLA-A*0201 is considered a prototype A2 supertype molecule).

[0528] These peptides are then tested for the capacity to bind to additional A2-supertype molecules (A*0202, A*0203, A*0206, and A*6802). Peptides that bind to at least three of the five A2-supertype alleles tested are typically deemed A2-supertype cross-reactive binders. Preferred peptides bind at an affinity equal to or less than 500 nM to three or more HLA-A2 supertype molecules.

[0529] Selection of HLA-A3 Supermotif-Bearing Epitopes

[0530] The 121P1F1 protein sequence(s) scanned above is also examined for the presence of peptides with the HILA-A3-supermotif primary anchors. Peptides corresponding to the HLA A3 supermotif-bearing sequences are then synthesized and tested for binding to HLA-A*0301 and HLA-A* 1101 molecules, the molecules encoded by the two most prevalent A3-supertype alleles. The peptides that bind at least one of the two alleles with binding affinities of <500 nM, often <200 nM, are then tested for binding cross-reactivity to the other common A3-supertype alleles (e.g., A*3101, A*3301, and A*6801) to identify those that can bind at least three of the five HLA-A3-supertype molecules tested.

[0531] Selection of HLA-B7 Supermotif Bearing Epitopes

[0532] The 121P1F1 protein(s) scanned above is also analyzed for the presence of 8-, 9-10-, or 11-mer peptides with the HLA-B7-supermotif. Corresponding peptides are synthesized and tested for binding to HLA-B*0702, the molecule encoded by the most common B7-supertype allele (i.e., the prototype B7 supertype allele). Peptides binding B*0702 with IC₅₀ of <500 nM are identified using standard methods. These peptides are then tested for binding to other common B7-supertype molecules (e.g., B*3501, B*5101, B*5301, and B*5401). Peptides capable of binding to three or more of the five B7-supertype alleles tested are thereby identified.

[0533] Selection of A1 and A24 Motif-Bearing Epitopes

[0534] To further increase population coverage, HLA-A1 and -A24 epitopes can also be incorporated into vaccine compositions. An analysis of the 121P1F1 protein can also be performed to identify HLA-A1- and A24-motif-containing sequences.

[0535] High affinity and/or cross-reactive binding epitopes that bear other motif and/or supermotifs are identified using analogous methodology.

Example 13 Confirmation of Immunogenicity

[0536] Cross-reactive candidate CTL A2-supermotif-bearing peptides that are identified as described herein are selected to confirm in vitro immunogenicity. Confirmation is performed using the following methodology:

[0537] Target Cell Lines for Cellular Screening:

[0538] The 0.221A2.1 cell line, produced by transferring the HLA-A2.1 gene into the HLA-A, -B, -C null mutant human B-lymphoblastoid cell line 721.221, is used as the peptide-loaded target to measure activity of HLA-A2. l-restricted CTL. This cell line is grown in RPMI-1640 medium supplemented with antibiotics, sodium pyruvate, nonessential amino acids and 10% (v/v) heat inactivated FCS. Cells that express an antigen of interest, or transfectants comprising the gene encoding the antigen of interest, can be used as target cells to confirm the ability of peptide-specific CTLs to recognize endogenous antigen.

[0539] Primary CTL Induction Cultures:

[0540] Generation of Dendritic Cells (DC): PBMCs are thawed in RPMI with 30 μg/ml DNAse, washed twice and resuspended in complete medium (RPMI-1640 plus 5% AB human serum, non-essential amino acids, sodium pyruvate, L-glutamine and penicillin/streptomycin). The monocytes are purified by plating 10×10⁶ PBMC/well in a 6-well plate. After 2 hours at 37° C., the non-adherent cells are removed by gently shaking the plates and aspirating the supernatants. The wells are washed a total of three times with 3 ml RPMI to remove most of the non-adherent and loosely adherent cells. Three ml of complete medium containing 0.50 ng/ml of GM-CSF and 1,000 U/ml of IL-4 are then added to each well. TNFα is added to the DCs on day 6 at 75 ng/ml and the cells are used for CTL induction cultures on day 7.

[0541] Induction of CTL with DC and Peptide: CD8+ T-cells are isolated by positive selection with Dynal immunomagnetic beads (Dynabeads® M-450) and the detacha-bead® reagent. Typically about 200-250×10⁶ PBMC are processed to obtain 24×10⁶ CD8⁺ T-cells (enough for a 48-well plate culture). Briefly, the PBMCs are thawed in RPMI with 30 μg/ml DNAse, washed once with PBS containing 1% human AB serum and resuspended in PBS/1% AB serum at a concentration of 20×10⁶cells/ml. The magnetic beads are washed 3 times with PBS/AB serum, added to the cells (140 μl beads/20×10⁶ cells) and incubated for 1 hour at 4° C. with continuous mixing. The beads and cells are washed 4× with PBS/AB serum to remove the nonadherent cells and resuspended at 100×10⁶ cells/ml (based on the original cell number) in PBS/AB serum containing 100 μl/ml detacha-bead® reagent and 30 μg/mil DNAse. The mixture is incubated for 1 hour at room temperature with continuous mixing. The beads are washed again with PBS/AB/DNAse to collect the CD8+ T-cells. The DC are collected and centrifuged at 1300 rpm for 5-7 minutes, washed once with PBS with 1% BSA, counted and pulsed with 40 μg/ml of peptide at a cell concentration of 1-2×10⁶/ml in the presence of 3 μg/ml β₂-microglobulin for 4 hours at 20° C. The DC are then irradiated (4,200 rads), washed 1 time with medium and counted again.

[0542] Setting up induction cultures: 0.25 ml cytokine-generated DC (at 1×10⁵ cells/ml) are co-cultured with 0.25 ml of CD8+ T-cells (at 2×10⁶ cell/ml) in each well of a 48-well plate in the presence of 10 ng/ml of IL-7. Recombinant human IL-10 is added the next day at a final concentration of 10 ng/ml and rhuman IL-2 is added 48 hours later at 10 IU/ml.

[0543] Restimulation of the induction cultures with peptide-pulsed adherent cells: Seven and fourteen days after the primary induction, the cells are restimulated with peptide-pulsed adherent cells. The PBMCs are thawed and washed twice with RPMI and DNAse. The cells are resuspended at 5×10⁶ cells/ml and irradiated at ˜4200 rads. The PBMCs are plated at 2×10⁶ in 0.5 ml complete medium per well and incubated for 2 hours at 37° C. The plates are washed twice with RPMI by tapping the plate gently to remove the nonadherent cells and the adherent cells pulsed with 10 μg/ml of peptide in the presence of 3 μg/ml β₂ microglobulin in 0.25 ml RPMI/5% AB per well for 2 hours at 37° C. Peptide solution from each well is aspirated and the wells are washed once with RPMI. Most of the media is aspirated from the induction cultures (CD8+ cells) and brought to 0.5 ml with fresh media. The cells are then transferred to the wells containing the peptide-pulsed adherent cells. Twenty four hours later recombinant human IL-10 is added at a final concentration of 10 ng/ml and recombinant human IL2 is added the next day and again 2-3 days later at 50IU/ml (Tsai et al, Critical Reviews in Immunology 18(1-2):65-75, 1998). Seven days later, the cultures are assayed for CTL activity in a ⁵¹Cr release assay. In some experiments the cultures are assayed for peptide-specific recognition in the in situ IFNγ ELISA at the time of the second restimulation followed by assay of endogenous recognition 7 days later. After expansion, activity is measured in both assays for a. side-by-side comparison.

[0544] Measurement of CTL Lytic Activity by ⁵¹Cr Release.

[0545] Seven days after the second restimulation, cytotoxicity is determined in a standard (5 hr) ⁵ Cr release assay by assaying individual wells at a single E:T. Peptide-pulsed targets are prepared by incubating the cells with 10 μg/ml peptide overnight at 37° C.

[0546] Adherent target cells are removed from culture flasks with trypsin-EDTA. Target cells are labeled with 200 μCi of ⁵¹Cr sodium chromate (Dupont, Wilmington, Del.) for 1 hour at 37° C. Labeled target cells are resuspended at 10⁶ per ml and diluted 1:10 with K562 cells at a concentration of 3.3×10⁶/ml (an NK-sensitive erythroblastoma cell line used to reduce non-specific lysis). Target cells (100 μl) and effectors (100 μl) are plated in 96 well round-bottom plates and incubated for 5 hours at 37° C. At that time, 100 μl of supernatant are collected from each well and percent lysis is determined according to the formula: [(cpm of th test sample-cpm of the spontaneous ⁵¹Cr release sample)/(cpm of the maximal ⁵¹Cr release sample-cpm of the spontaneous ⁵¹Cr release sample)]×100.

[0547] Maximum and spontaneous release are determined by incubating the labeled targets with 1% Triton X-1100 and media alone, respectively. A positive culture is defined as one in which the specific lysis (sample-background) is 10% or higher in the case of individual wells and is 15% or more at the two highest E:T ratios when expanded cultures are assayed.

[0548] In situ Measurement of Human IFNγ Production as an Indicator of Peptide-Specific and Endogenous Recognition

[0549] Immulon 2 plates are coated with mouse anti-human IFNγ monoclonal antibody (4 μg/ml 0.1M NaHCO₃, pH8.2) overnight at 4° C. The plates are washed with Ca²⁺, Mg²⁺-free PBS/0.05% Tween 20 and blocked with PBS/10% FCS for two hours, after which the CTLs (100 μl/well) and targets (100 μl/well) are added to each well, leaving empty wells for the standards and blanks (which received media only). The target cells, either peptide-pulsed or endogenous targets, are used at a concentration of 1×10⁶ cells/ml. The plates are incubated for 48 hours at 37° C. with 5% CO₂.

[0550] Recombinant human IFN-gamma is added to the standard wells starting at 400 pg or 1200 pg/100 microliter/well and the plate incubated for two hours at 37° C. The plates are washed and 100 μl of biotinylated mouse anti-human IFN-gamma monoclonal antibody (2 microgram/ml in PBS/3% FCS/0.05% Tween 20) are added and incubated for 2 hours at room temperature. After washing again, 100 microliter HRP-streptavidin (1:4000) are added and the plates incubated for one hour at room temperature. The plates are then washed 6× with wash buffer, 100 microliter/well developing solution (TMB 1:1) are added, and the plates allowed to develop for 5-15 minutes. The reaction is stopped with 50 microliter/well 1M H₃PO4 and read at OD450. A culture is considered positive if it measured at least 50 pg of IFN-gamma/well above background and is twice the background level of expression.

[0551] CTL Expansion.

[0552] Those cultures that demonstrate specific lytic activity against peptide-pulsed targets and/or tumor targets are expanded over a two week period with anti-CD3. Briefly, 5×10⁴ CD8+cells are added to a T25 flask containing the following: 1×10⁶ irradiated (4,200 rad) PBMC (autologous or allogeneic) per ml, 2×10⁵ irradiated (8,000 rad) EBV-transformed cells per ml, and OKT3 (anti-CD3) at 30 ng per ml in RPMI-1640 containing 10% (v/v) human AB serum, non-essential amino acids, sodium pyruvate, 25 μM 2-mercaptoethanol, L-glutamine and penicillin/streptomycin. Recombinant human IL2 is added 24 hours later at a final concentration of 2001U/ml and every three days thereafter with fresh media at 50IU/ml. The cells are split if the cell concentration exceeds 1×10⁶/ml and the cultures are assayed between days 13 and 15 at E:T ratios of 30, 10, 3 and 1:1 in the ⁵¹Cr release assay or at 1×10⁶/ml in the in situ IFNγ assay using the same targets as before the expansion.

[0553] Cultures are expanded in the absence of anti-CD3⁺ as follows. Those cultures that demonstrate specific lytic activity against peptide and endogenous targets are selected and 5×10⁴ CD8+cells are added to a T25 flask containing the following: 1×10⁶ autologous PBMC per ml which have been peptide-pulsed with 10 μg/ml peptide for two hours at 37° C. and irradiated (4,200 rad); 2×10⁵ irradiated (8,000 rad) EBV-transformed cells per ml RPMI-1640 containing 10% (v/v) human AB serum, non-essential AA, sodium pyruvate, 25 mM 2-ME, L-glutamine and gentamicin.

[0554] Immunogenicity of A2 Supermotif-Bearing Peptides

[0555] A2-supermotif cross-reactive binding peptides are tested in the cellular assay for the ability to induce peptide-specific CTL in normal individuals. In this analysis, a peptide is typically considered to be an epitope if it induces peptide-specific CTLs in at least individuals, and preferably, also recognizes the endogenously expressed peptide.

[0556] Immunogenicity can also be confirmed using PBMCs isolated from patients bearing a tumor that expresses 121P1F1. Briefly, PBMCs are isolated from patients, re-stimulated with peptide-pulsed monocytes and assayed for the ability to recognize peptide-pulsed target cells as well as transfected cells endogenously expressing the antigen.

[0557] Evaluation of A*03/A11 immunogenicity HLA-A3 supermotif-bearing cross-reactive binding peptides are also evaluated for immunogenicity using methodology analogous for that used to evaluate the immunogenicity of the HLA-A2 supermotif peptides.

[0558] Evaluation of B7 Immunogenicity

[0559] Immunogenicity screening of the B7-supertype cross-reactive binding peptides identified as set forth herein are confirmed in a manner analogous to the confirmation of A2-and A3-supermotif-bearing peptides.

[0560] Peptides bearing other supermotifs/motifs, e.g., HLA-A1, HLA-A24 etc. are also confirmed using similar methodology

Example 14 Implementation of the Extended Supermotif to Improve the Binding Capacity of Native Epitopes by Creating Analogs

[0561] HLA motifs and supermotifs (comprising primary and/or secondary residues) are useful in the identification and preparation of highly cross-reactive native peptides, as demonstrated herein. Moreover, the definition of HLA motifs and supermotifs also allows one to engineer highly cross-reactive epitopes by identifying residues within a native peptide sequence which can be analoged to confer upon the peptide certain characteristics, e.g. greater cross-reactivity within the group of HLA molecules that comprise a supertype, and/or greater binding affinity for some or all of those HLA molecules. Examples of analoging peptides to exhibit modulated binding affinity are set forth in this example.

[0562] Analoging at Primary Anchor Residues

[0563] Peptide engineering strategies are implemented to further increase the cross-reactivity of the epitopes. For example, the main anchors of A2-supermotif-bearing peptides are altered, for example, to introduce a preferred L, I, V, or M at position 2, and I or V at the C-terminus.

[0564] To analyze the cross-reactivity of the analog peptides, each engineered analog is initially tested for binding to the prototype A2 supertype allele A*0201, then, if A*0201 binding capacity is maintained, for A2-supertype cross-reactivity.

[0565] Alternatively, a peptide is confirmed as binding one or all supertype members and then analoged to modulate binding affinity to any one (or more) of the supertype members to add population coverage.

[0566] The selection of analogs for immunogenicity in a cellular screening analysis is typically further restricted by the capacity of the parent wild type (WT) peptide to bind at least weakly, i.e., bind at an IC₅₀ of 5000 nM or less, to three of more A2 supertype alleles. The rationale for this requirement is that the WT peptides must be present endogenously in sufficient quantity to be biologically relevant. Analoged peptides have been shown to have increased immunogenicity and cross-reactivity by T cells specific for the parent epitope (see, e.g., Parkhurst et al., J. Immunol. 157:2539, 1996; and Pogue et al., Proc. Natl. Acad. Sci. USA 92:8166, 1995).

[0567] In the cellular screening of these peptide analogs, it is important to confirm that analog-specific CTLs are also able to recognize the wild-type peptide and, when possible, target cells that endogenously express the epitope.

[0568] Analoging of HLA-A3 and B7-Supermotif-Bearing Peptides

[0569] Analogs of HLA-A3 supermotif-bearing epitopes are generated using strategies similar to those employed in analoging HLA-A2 supermotif-bearing peptides. For example, peptides binding to 3/5 of the A3-supertype molecules are engineered at primary anchor residues to possess a preferred residue (V, S, M, or A) at position 2.

[0570] The analog peptides are then tested for the ability to bind A*03 and A*11 (prototype A3 supertype alleles). Those peptides that demonstrate≦500 nM binding capacity are then confirmed as having A3-supertype cross-reactivity.

[0571] Similarly to the A2- and A3-motif bearing peptides, peptides binding 3 or more B7-supertype alleles can be improved, where possible, to achieve increased cross-reactive binding or greater binding affinity or binding half life. B7 supermotif-bearing peptides are, for example, engineered to possess a preferred residue (V, I, L, or F) at the C-terminal primary anchor position, as demonstrated by Sidney. et al. (J. Immunol. 157:3480-3490, 1996).

[0572] Analoging at primary anchor residues of other motif and/or supermotif-bearing epitopes is performed in a like manner.

[0573] The analog peptides are then be confirmed for immunogenicity, typically in a cellular screening assay. Again, it is generally important to demonstrate that analog-specific CTLs are also able to recognize the wild-type peptide and, when possible, targets that endogenously express the epitope.

[0574] Analoging at Secondary Anchor Residues

[0575] Moreover, HLA supermotifs are of value in engineering highly cross-reactive peptides and/or peptides that bind HLA molecules with increased affinity by identifying particular residues at secondary anchor positions that are associated with such properties. For example, the binding capacity of a B7 supermotif-bearing peptide with an F residue at position 1 is analyzed. The peptide is then analoged to, for example, substitute L for F at position 1. The analoged peptide is evaluated for increased binding affinity, binding half life and/or increased cross-reactivity. Such a procedure identifies analoged peptides with enhanced properties.

[0576] Engineered analogs with sufficiently improved binding capacity or cross-reactivity can also be tested for immunogenicity in HLA-B7-transgenic mice, following for example, IFA immunization or lipopeptide immunization. Analoged peptides are additionally tested for the ability to stimulate a recall response using PBMC from patients with 121P1F1-expressing tumors.

[0577] Other Analoging Strategies

[0578] Another form of peptide analoging, unrelated to anchor positions, involves the substitution of a cysteine with α-amino butyric acid. Due to its chemical nature, cysteine has the propensity to form disulfide bridges and sufficiently alter the peptide structurally so as to reduce binding capacity. Substitution of a-amino butyric acid for cysteine not only alleviates this problem, but has been shown to improve binding and crossbinding capabilities in some instances (see, e.g., the review by Sette et al., In: Persistent Viral Infections, Eds. R. Ahmed and I. Chen, John Wiley & Sons, England, 1999).

[0579] Thus, by the use of single amino acid substitutions, the binding properties and/or cross-reactivity of peptide ligands for HLA supertype molecules can be modulated.

Example 15 Identification and Confirmation of 121P1F1-Derived Sequences with HLA-DR Binding Motifs

[0580] Peptide epitopes bearing an HLA class II supermotif or motif are identified and confirmed as outlined below using methodology similar to that described for HLA Class I peptides.

[0581] Selection of HLA-DR-Supermotif-Bearing Epitopes.

[0582] To identify 121P1F1-derived, HLA class II HTL epitopes, a 121P1F1 antigen is analyzed for the presence of sequences bearing an HLA-DR-motif or supermotif. Specifically, 15-mer sequences are selected comprising a DR-supermotif, comprising a 9-mer core, and three-residue N- and C-terminal flanking regions (15 amino acids total).

[0583] Protocols for predicting peptide binding to DR molecules have been developed (Southwood et al., J. Immunol. 160:3363-3373, 1998). These protocols, specific for individual DR molecules, allow the scoring, and ranking, of 9-mer core regions. Each protocol not only scores peptide sequences for the presence of DR-supermotif primary anchors (i.e., at position 1 and position 6) within a 9-mer core, but additionally evaluates sequences for the presence of secondary anchors. Using allele-specific selection tables (see, e.g., Southwood et al., ibid.), it has been found that these protocols efficiently select peptide sequences with a high probability of binding a particular DR molecule. Additionally, it has been found that performing these protocols in tandem, specifically those for DR1, DR4w4, and DR7, can efficiently select DR cross-reactive peptides.

[0584] The 121P1F1-derived peptides identified above are tested for their binding capacity for various common HLA-DR molecules. All peptides are initially tested for binding to the DR molecules in the primary panel: DR1, DR4w4, and DR7. Peptides binding at least two of these three DR molecules are then tested for binding to DR2w2 β1, DR2w2 β2, DR6w19, and DR9 molecules in secondary assays. Finally, peptides binding at least two of the four secondary panel DR molecules, and thus cumulatively at least four of seven different DR molecules, are screened for binding to DR4w15, DR5w11, and DR8w2 molecules in tertiary assays. Peptides binding at least seven of the ten DR molecules comprising the primary, secondary, and tertiary screening assays are considered cross-reactive DR binders. 121P1F1-derived peptides found to bind common HLA-DR alleles are of particular interest.

[0585] Selection of DR3 Motif Peptides

[0586] Because HLA-DR3 is an allele that is prevalent in Caucasian, Black, and Hispanic populations, DR3 binding capacity is a relevant criterion in the selection of HTL epitopes. Thus, peptides shown to be candidates may also be assayed for their DR3 binding capacity. However, in view of the binding specificity of the DR3 motif, peptides binding only to DR3 can also be considered as candidates for inclusion in a vaccine formulation.

[0587] To efficiently identify peptides that bind DR3, target 121P1F1 antigens are analyzed for sequences carrying ore of the two DR3-specific binding motifs reported by Geluk et al (J. Immunol. 152:5742-5748, 1994). The corresponding peptides are then synthesized and confirmed as having the ability to bind DR3 with an affinity of 1 μM or better, i.e., less than 1 μM. Peptides are found that meet this binding criterion and qualify as HLA class II high affinity binders.

[0588] DR3 binding epitopes identified in this manner are included in vaccine compositions with DR supermotif-bearing peptide epitopes.

[0589] Similarly to the case of HLA class I motif-bearing peptides, the class II motif-bearing peptides are analoged to improve affinity or cross-reactivity. For example, aspartic acid at position 4 of the 9-mer core sequence is an optimal residue for DR3 binding, and substitution for that residue often improves DR 3 binding.

[0590] Example 16

Immunogenicity of 121P1F1-Derived HTL Epitopes

[0591] This example determines immunogenic DR supermotif- and DR3 motif-bearing epitopes among those identified using the methodology set forth herein.

[0592] Immunogenicity of HTL epitopes are confirmed in a manner analogous to the determination of immunogenicity of CTL epitopes, by assessing the ability to stimulate HTL responses and/or by using appropriate transgenic mouse models. Immunogenicity is determined by screening for: 1.) in vitro primary induction using normal PBMC or 2.) recall responses from patients who have 121P1F1-expressing tumors.

Example 17 Calculation of Phenotypic Frequencies of HLA-Supertypes in Various Ethnic Backgrounds to Determine Breadth of Population Coverage

[0593] This example illustrates the assessment of the breadth of population coverage of a vaccine composition comprised of multiple epitopes comprising multiple supermotifs and/or motifs.

[0594] In order to analyze population coverage, gene frequencies of HLA alleles are determined. Gene frequencies for each HLA allele are calculated from antigen or allele frequencies utilizing the binomial distribution formulae gf=1−(SQRT(1−af)) (see, e.g., Sidney et al., Human Immunol 45:79-93, 1996). To obtain overall phenotypic frequencies, cumulative gene frequencies are calculated, and the cumulative antigen frequencies derived by the use of the inverse formula [af=1−(1−Cgf)²].

[0595] Where frequency data is not available at the level of DNA typing, correspondence to the serologically defined antigen frequencies is assumed. To obtain total potential supertype population coverage no linkage disequilibrium is assumed, and only alleles confirmed to belong to each of the supertypes are included (minimal estimates). Estimates of total potential coverage achieved by inter-loci combinations are made by adding to the A coverage the proportion of the non-A covered population that could be expected to be covered by the B alleles considered (e.g., total=A+B*(1−A)). Confirmed members of the A3-like supertype are A3, A11, A31, A*3301, and A*6801. Although the A3-like supertype may also include A34, A66, and A*7401, these alleles were not included in overall frequency calculations. Likewise, confirmed members of the A2-like supertype family are A*0201, A*0202, A*0203, A*0204, A*0205, A*0206, A*0207, A*6802, and A*6901. Finally, the B7-like supertype-confirmed alleles are: B7. B*3501-03, B51, B*5301, B*5401, B*5501-2, B*5601, B*6701, and B*7801 (potentially also B*1401, B*3504-06, B*4201, and B*5602).

[0596] Population coverage achieved by combining the A2-, A3- and B7-supertypes is approximately 86% in five major ethnic groups. Coverage may be extended by including peptides bearing the A1 and A24 motifs. On average, A1 is present in 12% and A24 in 29% of the population across five different major ethnic groups (Caucasian, North American Black, Chinese, Japanese, and Hispanic). Together, these alleles are represented with an average frequency of 39% in these same ethnic populations. The total coverage across the major ethnicities when A1 and A24 are combined with the coverage of the A2-, A3- and B7-supertype alleles is >95%. An analogous approach can be used to estimate population coverage achieved w ith combinations of class II motif-bearing epitopes.

[0597] Immunogenicity studies in humans (e.g., Bertoni et al., J. Clin. Invest. 100:503, 1997; Doolan et al., Immunity 7:97, 1997; and Threlkeld et al., J. Immunol. 159:1648, 1997) have shown that highly cross-reactive binding peptides are almost always recognized as epitopes. The use of highly cross-reactive binding peptides is an important selection criterion in identifying candidate epitopes for inclusion in a vaccine that is immunogenic in a diverse population.

[0598] With a sufficient number of epitopes (as disclosed herein and from the art), an average population coverage is, predicted to be greater than 95% in each of five major ethnic populations. The game theory Monte Carlo simulation analysis, which is known in the art (see e.g., Osborne, M. J. and Rubinstein, A. “A course in game theory” MIT Press, 1994), can be used to estimate what percentage of the individuals in a population comprised of the Caucasian, North American Black, Japanese, Chinese, and Hispanic ethnic groups would recognize the vaccine epitopes described herein. A preferred percentage is 90%. A more preferred percentage is 95%.

Example 18 CTL Recognition of Endogenously Processed Antigens After Priming

[0599] This example confirms that CTL induced by native or analoged peptide epitopes identified and selected as described herein recognize endogenously synthesized, i.e., native antigens.

[0600] Effector cells isolated from transgenic mice that are immunized with peptide epitopes, for example HLA-A2 supermotif-bearing epitopes, are re-stimulated in vitro using peptide-coated stimulator cells. Six days later, effector cells are assayed for cytotoxicity and the cell lines that contain peptide-specific cytotoxic activity are further re-stimulated. An additional six days later, these cell lines are tested for cytotoxic activity on ⁵¹Cr labeled Jurkat-A2.1/K^(b) target cells in the absence or presence of peptide, and also tested on ⁵¹Cr labeled target cells bearing the endogenously synthesized antigen, i.e. cells that are stably transfected with 121P1F1 expression vectors.

[0601] The results demonstrate that CTL lines obtained from animals primed with peptide epitope recognize endogenously synthesized 121P1F1 antigen. The choice of transgenic mouse model to be used for such an analysis depends upon the epitope(s) that are being evaluated. In addition to HLA-A*0201K K^(b) transgenic mice, several other transgenic mouse models including mice with human A11, which may also be used to evaluate A3 epitopes, and B7 alleles have been characterized and others (e.g., transgenic mice for HLA-A1 and A24) are being developed. HLA-DR1 and HLA-DR3 mouse models have also been developed, which may be used to evaluate HTL epitopes.

Example 19 Activity Of CTL-HTL Conjugated Epitopes In Transgenic Mice

[0602] This example illustrates the induction of CTLs and HTLs in transgenic mice, by use of a 121P1F1-derived CTL and HTL peptide vaccine compositions. The vaccine composition used herein comprise peptides to be administered to a patient with a 121P1F1-expressing tumor. The peptide composition can comprise multiple CTL and/or HTL epitopes. The epitopes are identified using methodology as described herein. This example also illustrates that enhanced immunogenicity can be achieved by inclusion of one or more HTL epitopes in a CTL vaccine composition; such a peptide composition can comprise an HTL epitope conjugated to a CTL epitope. The CTL epitope can be one that binds to multiple HLA family members at an affinity of 500 nM or less, or analogs of that epitope. The peptides may be lipidated, if desired.

[0603] Immunization procedures: Immunization of transgenic mice is performed as described (Alexander et al., J Immunol. 159:4753-4761, 1997). For example, A2/K^(b) mice, which are transgenic for the human HLA A2.1 allele and are used to confirm the immunogenicity of HLA-A*0201 motif- or HLA-A2 supermotif-bearing epitopes, and are primed subcutaneously (base of the tail) with a 0.1 ml of peptide in Incomplete Freund's Adjuvant, or if the peptide composition is a lipidated CTL/HTL conjugate, in DMSO/saline, or if the peptide composition is a polypeptide, in PBS or Incomplete Freund's Adjuvant. Seven days after priming, splenocytes obtained from these animals are restimulated with syngenic irradiated LPS-activated lymphoblasts coated with peptide.

[0604] Cell lines: Target cells for peptide-specific cytotoxicity assays are Jurkat cells transfected with the HLA-A2.1/K^(b) chimeric gene (e.g., Vitiello et al., J. Exp. Med. 173:1007, 1991)

[0605] In vitro CTL activation: One week after priming, spleen cells (30×10⁶ cells/flask) are co-cultured at 37° C. with syngeneic, irradiated (3000 rads), peptide coated lymphoblasts (10×10⁶ cells/flask) in 10 ml of culture medium/T25 flask. After six days, effector cells are harvested and assayed for cytotoxic activity.

[0606] Assay for cytotoxic activity: Target cells (1.0 to 1.5×10⁶) are incubated at 37° C. in the presence of 200 μl of ⁵¹Cr. After 60 minutes, cells are washed three times and resuspended in R10 medium. Peptide is added where required at a concentration of 1 μg/ml. For the assay, 10⁴ ⁵¹Cr-labeled target cells are added to different concentrations of effector cells (final volume of 200 μl) in U-bottom 96-well plates. After a six hour incubation period at 37° C., a 0.1 ml aliquot of supernatant is removed from each well and radioactivity is determined in a Micromedic automatic gamma counter. The percent specific lysis is determined by the formula: percent specific release=100×(experimental release−spontaneous release)/(maximum release−spontaneous release). To facilitate comparison between separate CTL assays run under the same conditions, % ⁵¹Cr release data is expressed as lytic units/10⁶ cells. One lytic unit is arbitrarily defined as the number of effector cells required to achieve 30° O % lysis of 10,000 target cells in a six hour ⁵¹Cr release assay. To obtain specific lytic units/10⁶, the lytic units/10⁶ obtained in the absence of peptide is subtracted from the lytic units/10⁶ obtained in the presence of peptide. For example, if 30% ⁵¹Cr release is obtained at the effector (E): target (T) ratio of 50:1 (i.e., 5×10⁵ effector cells for 10,000 targets) in the absence of peptide and 5:1 (i.e., 5×10⁴ effector cells for 10,000 targets) in the presence of peptide, the specific lytic units would be: [(1/50,000)−(1/500,000)]×10⁶=18 LU.

[0607] The results are analyzed to assess the magnitude of the CTL responses of animals injected with the immunogenic CTL/HTL conjugate vaccine preparation and are compared to the magnitude of the CTL response achieved using, for example, CTL epitopes as outlined above in the Example entitled “Confirmation of Immunogenicity”. Analyses similar to this may be performed to confirm the immunogenicity of peptide conjugates containing multiple CTL epitopes and/or multiple HTL epitopes. In accordance with these procedures, it is found that a CTL response is induced, and concomitantly that an HTL response is induced upon administration of such compositions.

Example 20 Selection of CTL and HTL Epitopes for Inclusion in an 121P1F1-Specific Vaccine.

[0608] This example illustrates a procedure for selecting peptide epitopes for vaccine compositions of the invention. The peptides in the composition can be in the form of a nucleic acid sequence, either single or one or more sequences (i.e., minigene) that encodes peptide(s), or can be single and/or polyepitopic peptides.

[0609] The following principles are utilized when selecting a plurality of epitopes for inclusion in a vaccine composition. Each of the following principles is balanced in order to make the selection.

[0610] Epitopes are selected which, upon administration, mimic immune responses that are correlated with 121P1F1 clearance. The number of epitopes used depends on observations of patients who spontaneously clear 121P1F1. For example, if it has been observed that patients who spontaneously clear 121P1F1 generate an immune response to at least three (3) from 121P1F1 antigen, then three or four (34) epitopes should be included for HLA class I. A similar rationale is used to determine HLA class II epitopes.

[0611] Epitopes are often selected that have a binding affinity of an IC₅₀ of 500 nM or less for an HLA class I molecule, or for class II, an IC₅₀ of 1000 nM or less; or HLA Class I peptides with high binding scores from the BIMAS web site, at URL bimas.dcrt.nih.gov/.

[0612] In order to achieve broad coverage of the vaccine through out a diverse population, sufficient supermotif bearing peptides, or a sufficient array of allele-specific motif bearing peptides, are selected to give broad population coverage. In one embodiment, epitopes are selected to provide at least 80% population coverage. A Monte Carlo analysis, a statistical evaluation known in the art, can be employed to assess breadth, or redundancy, of population coverage.

[0613] When creating polyepitopic compositions, or a minigene that encodes same, it is typically desirable to generate the smallest peptide possible that encompasses the epitopes of interest. The principles employed are similar, if not the same, as those employed when selecting a peptide comprising nested epitopes. For example, a protein sequence for the vaccine composition is selected because it has maximal number of epitopes contained within the sequence, i.e., it has a high concentration of epitopes. Epitopes may be nested or overlapping (i.e., frame shifted relative to one another). For example, with overlapping epitopes, two 9-mer epitopes and one 10-mer epitope can be present in a 10 amino acid peptide. Each epitope can be exposed and bound by an HLA molecule upon administration of such a peptide. A multi-epitopic, peptide can be generated synthetically, recombinantly, or via cleavage from the native source. Alternatively, an analog can be made of this native sequence, whereby one or more of the epitopes comprise substitutions that alter the cross-reactivity and/or binding affinity properties of the polyepitopic peptide. Such a vaccine composition is administered for therapeutic or prophylactic purposes. This embodiment provides for the possibility that an as yet undiscovered aspect of immune system processing will apply to the native nested sequence and thereby facilitate the production of therapeutic or prophylactic immune response-inducing vaccine compositions. Additionally such an embodiment provides for the possibility of motif-bearing epitopes for an HLA makeup that is presently unknown. Furthermore, this embodiment (absent the creating of any analogs) directs the immune response to multiple peptide sequences that are actually present in 121P1F1, thus avoiding the need to evaluate any junctional epitopes. Lastly, the embodiment provides an economy of scale when producing nucleic acid vaccine compositions. Related to this embodiment, computer programs can be derived in accordance with principles in the art, which identify in a target sequence, the greatest number of epitopes per sequence length.

[0614] A vaccine composition comprised of selected peptides, when administered, is safe, efficacious, and elicits an immune response similar in magnitude to an immune response that controls or clears cells that bear or overexpress 121P1F1.

[0615] Example 21: Construction of “Minigene” Multi-Epitope DNA Plasmids

[0616] This example discusses the construction of a minigene expression plasmid. Minigene plasmids may, of course, contain various configurations of B cell, CTL and/or HTL epitopes or epitope analogs as described herein.

[0617] A minigene expression plasmid typically includes multiple CTL and HTL peptide epitopes. In the present example, HLA-A2, -A3, -B7 supermotif-bearing peptide epitopes and HLA-Al and -A24 motif-bearing peptide epitopes are used in conjunction with DR supermotif-bearing epitopes and/or DR3 epitopes. HLA class I supermotif or motif-bearing peptide epitopes derived 121P1F1, are selected such that multiple supermotifs/motifs are represented to ensure broad population coverage. Similarly, HLA class II epitopes are selected from 121P1F1 to provide broad population coverage, i.e. both HLA DR-1-4-7 supermotif-bearing epitopes and HLA DR-3 motif-bearing epitopes are selected for inclusion in the minigene construct. The selected CTL and HTL epitopes are then incorporated into a minigene for expression in an expression vector.

[0618] Such a construct may additionally include sequences that direct the HTL epitopes to the endoplasmic reticulum. For example, the II protein may be fused to one or more HTL epitopes as described in the art, wherein the CLIP sequence of the II protein is removed and replaced with an HLA class II epitope sequence so that HLA class II epitope is directed to the endoplasmic reticulum, where the epitope binds to an HLA class II molecules.

[0619] This example illustrates the methods to be used for construction of a minigene-bearing expression plasmid. Other expression vectors that may be used for minigene compositions are available and known to those of skill in the art.

[0620] The minigene DNA plasmid of this example contains a consensus Kozak sequence and a consensus murine kappa Ig-light chain signal sequence followed by CTL and/or HTL epitopes selected in accordance with principles disclosed herein. The sequence encodes an open reading frame fused to the Myc and His antibody epitope tag coded for by the pcDNA 3.1 Myc-His vector.

[0621] Overlapping oligonucleotides that can, for example, average about 70 nucleotides in length with 15 nucleotide overlaps, are synthesized and HPLC-purified. The oligonucleotides encode the selected peptide epitopes as well as appropriate linker nucleotides, Kozak sequence, and signal sequence. The final multiepitope minigene is assembled by extending the overlapping oligonucleotides in three sets of reactions using PCR. A Perkin/Elmer 9600 PCR machine is used and a total of 30 cycles are performed using the following conditions: 95° C. for 15 sec, annealing temperature (5° below the lowest calculated Tm of each primer pair) for 30 sec, and 72° C. for 1 min.

[0622] For example, a minigene is prepared as follows. For a first PCR reaction, 5 μg of each of two oligonucleotides are annealed and extended: In an example using eight oligonucleotides, i.e., four pairs of primers, oligonucleotides 1+2, 3+4, 5+6, and 7+8 are combined in 100 μl reactions containing Pfu polymerase buffer (1×=10 mM KCL, 10 mM (NH4)₂SO_(4, 20) mM Tris-chloride, pH 8.75, 2 mM MgSO₄, 0.1% Triton X-100, 100 μg/ml BSA), 0.25 mM each dNTP, and 2.5 U of Pfu polymerase. The full-length dimer products are gel-purified, and two reactions containing the product of 1+2 and 3+4, and the product of 5+6 and 7+8 are mixed, annealed, and extended for 10 cycles. Half of the two reactions are then mixed, and 5 cycles of annealing and extension carried out before flanking primers are added to amplify the full length product. The full-length product is gel-purified and cloned into pCR-blunt (Invitrogen) and individual clones are screened by sequencing.

Example 22 The Plasmid Construct and the Decree to Which It Induces Immunogenicity

[0623] The degree to which a plasmid construct, for example a plasmid constructed in accordance with the previous Example, is able to induce immunogenicity is confirmed in vitro by determining epitope presentation by APC following transduction or transfection of the APC with an epitope-expressing nucleic acid construct. Such a study determines “antigenicity” and allows the use of human APC. The assay determines the ability of the epitope to be presented by the APC in a context that is recognized by a T cell by quantifying the density of epitope-HLA class I complexes on the cell surface. Quantitation can be performed by directly measuring the amount of peptide eluted from the APC (see, e.g., Sijts et al., J. Immunol. 156:683-692, 1996; Demotz et al, Nature 342:682-684, 1989); or the number of peptide-HLA class I complexes can be estimated by measuring the amount of lysis or lymphokine release induced by diseased or transfected target cells, and then determining the concentration of peptide necessary to obtain equivalent levels of lysis or lymphokine release (see, e.g., Kageyama et al., J. Immunol. 154:567-576, 1995).

[0624] Alternatively, immunogenicity is confirmed through in vivo injections into mice and subsequent in vitro assessment of CTL and HTL activity, which are analyzed using cytotoxicity and proliferation assays, respectively, as detailed e.g., in Alexander et al., Immunity 1:751-761, 1994.

[0625] For example, to confirm the capacity of a DNA minigene construct containing at least one HLA-A2 supermotif peptide to induce CTLs in vivo, HLA-A2.₁/Kb transgenic mice, for example, are immunized intramuscularly with 100 μg of naked cDNA. As a means of comparing the level of CTLs induced by cDNA immunization, a control group of animals is also immunized with an actual peptide composition that comprises multiple epitopes synthesized as a single polypeptide as they would be encoded by the minigene.

[0626] Splenocytes from immunized animals are stimulated twice with each of the respective compositions (peptide epitopes encoded in the minigene or the polyepitopic peptide), then assayed for peptide-specific cytotoxic activity in a ⁵¹Cr release assay. The results indicate the magnitude of the CTL response directed against the A2-restricted epitope, thus indicating the in vivo immunogenicity of the minigene vaccine and polyepitopic vaccine.

[0627] It is, therefore, found that the minigene elicits immune responses directed toward the HLA-A2 supermotif peptide epitopes as does the polyepitopic peptide vaccine. A similar analysis is also performed using other HLA-A3 and HLA-B7 transgenic mouse models to assess CTL induction by HLA-A3 and HLA-B7 motif or supermotif epitopes, whereby it is also found that the minigene elicits appropriate immune responses directed toward the provided epitopes.

[0628] To confirm the capacity of a class II epitope-encoding minigene to induce HTLs in vivo, DR transgenic mice, or for those epitopes that cross react with the appropriate mouse MHC molecule, I-A^(b)-restricted mice, for example, are immunized intramuscularly with 100 μg of plasmid DNA. As a means of comparing the level of HTLs induced by DNA immunization, a group of control animals is also immunized with an actual peptide composition emulsified in complete Freund's adjuvant. CD4+ T cells, i.e. HTLs, are purified from splenocytes of immunized animals and stimulated with each of the respective compositions (peptides encoded in the minigene). The HTL response is measured using a ³H-thymidine incorporation proliferation assay, (see, e.g., Alexander et al. Immunity 1:751-761, 1994). The results indicate the magnitude of the HTL response, thus demonstrating the in vivo immunogenicity of the minigene.

[0629] DNA minigenes, constructed as described in the previous Example, can also be confirmed as a vaccine in combination with a boosting agent using a prime boost protocol. The boosting agent can consist of recombinant protein (e.g., Barnett et al., Aids Res. and Human Retroviruses 14, Supplement 3:S299-S309, 1998) or recombinant vaccinia, for example, expressing a minigene or DNA encoding the complete protein of interest (see, e.g., Hanke et al, Vaccine 16:439-445, 1998; Sedegah et al., Proc. Natl. Acad. Sci USA 95:7648-53, 1998; Hanke and McMichael, Immunol. Letters 66:177-181, 1999; and Robinson et al., Nature Med. 5:526-34, 1999).

[0630] For example, the efficacy of the DNA minigene used in a prime boost protocol is initially evaluated in transgenic mice. In this example, A2.1/K^(b) transgenic mice are immunized IM with 100 μg of a DNA minigene encoding the immunogenic peptides including at least one HLA-A2 supermotif-bearing peptide. After an incubation period (ranging from 3-9 weeks), the mice are boosted IP with 10⁷ pfu/mouse of a recombinant vaccinia virus expressing the same sequence encoded by the DNA minigene. Control mice are immunized with 100 μg of DNA or recombinant vaccinia without the minigene sequence, or with DNA encoding the minigene, but without the vaccinia boost. After an additional incubation period of two weeks, splenocytes from the mice are immediately assayed for peptide-specific activity in an ELISPOT assay. Additionally, splenocytes are stimulated in vitro with the A2-restricted peptide epitopes encoded in the minigene and recombinant vaccinia, then assayed for peptide-specific activity in an alpha, beta and/or gamma IFN ELISA.

[0631] It is found that the minigene utilized in a prime-boost protocol elicits greater immune responses toward the HLA-A2 supermotif peptides than with DNA alone. Such an analysis can also be performed using HLA-A11 or HLA-B7 transgenic mouse models to assess CTL induction by HLA-A3 or HLA-B7 motif or supermotif epitopes. The use of prime boost protocols in humans is described below in the Example entitled “Induction of CTL Responses Using a Prime Boost Protocol.”

Example 23 Peptide Compositions for Prophylactic Uses

[0632] Vaccine compositions of the present invention can be used to prevent 121P1F1 expression in persons who are at risk for tumors that bear this antigen. For example, a polyepitopic peptide epitope composition (or a nucleic acid comprising the same) containing multiple CTL and HTL epitopes such as those selected in the above Examples, which are also selected to target greater than 80% of the population, is administered to individuals at risk for a 121P1F1-associated tumor.

[0633] For example, a peptide-based composition is provided as a single polypeptide that encompasses multiple epitopes. The vaccine is typically administered in a physiological solution that comprises an adjuvant, such as Incomplete Freunds Adjuvant. The dose of peptide for the initial immunization is from about 1 to about 50,000 μg, generally 100-5,000 μg, for a 70 kg patient. The initial administration of vaccine is followed by booster dosages at 4 weeks followed by evaluation of the magnitude of the immune response in the patient, by techniques that determine the presence of epitope-specific CTL populations in a PBMC sample. Additional booster doses are administered as required. The composition is found to be both safe and efficacious as a prophylaxis against 121P1F1-associated disease.

[0634] Alternatively, a composition typically comprising transfecting agents is used for the administration of a nucleic acid-based vaccine in accordance with methodologies known in the art and disclosed herein.

Example 24 Polyepitopic Vaccine Compositions Derived from Native 121P1F1 Sequences

[0635] A native 121P1F1 polyprotein sequence is analyzed, preferably using computer algorithms defined for each class I and/or class II supermotif or motif, to identify “relatively short” regions of the polyprotein that comprise multiple epitopes. The “relatively short” regions are preferably less in length than an entire native antigen. This relatively short sequence that contains multiple distinct or overlapping, “nested” epitopes is selected; it can be used to generate a minigene construct. The construct is engineered to express the peptide, which corresponds to the native protein sequence. The “relatively short” peptide is generally less than 250 amino acids in length, often less than 100 amino acids in length, preferably less than 75 amino acids in length, and more preferably less than 50 amino acids in length. The protein sequence of the vaccine composition is selected because it has maximal number of epitopes contained within the sequence, ie., it has a high concentration of epitopes. As noted herein, epitope motifs may be nested or overlapping (i.e., frame shifted relative to one another). For example, with overlapping epitopes, two 9-mer epitopes and one 10-mer epitope can be present in a 10 amino acid peptide. Such a vaccine composition is administered for therapeutic or prophylactic purposes.

[0636] The vaccine composition will include, for example, multiple CTL epitopes from 121P1F1 antigen and at least one HTL epitope. This polyepitopic native sequence is administered either as a peptide or as a nucleic acid sequence which encodes the peptide. Alternatively, an analog can be made of this native sequence, whereby one or more of the epitopes comprise substitutions that alter the cross-reactivity and/or binding affinity properties of the polyepitopic peptide.

[0637] The embodiment of this example provides for the possibility that an as yet undiscovered aspect of immune system processing will apply to the native nested sequence and thereby facilitate the production of therapeutic or prophylactic immune response-inducing vaccine compositions. Additionally such an embodiment provides for the possibility of motif-bearing epitopes for an HLA makeup that is presently unknown. Furthermore, this embodiment (excluding an analoged embodiment) directs the immune response to multiple peptide sequences that are actually present in native 121P1F1, thus avoiding the need to evaluate any junctional epitopes. Lastly, the embodiment provides an economy of scale when producing peptide or nucleic acid vaccine compositions.

[0638] Related to this embodiment, computer programs are available in the art which can be used to identify in a target sequence, the greatest number of epitopes per sequence length.

Example 25 Polyepitopic Vaccine Compositions From Multiple Antigens

[0639] The 121P1F1 peptide epitopes of the present invention are used in conjunction with epitopes from other target tumor-associated antigens, to create a vaccine composition that is useful for the prevention or treatment of cancer that expresses 121P1F1 and such other antigens. For example, a vaccine composition can be provided as a single polypeptide that incorporates multiple epitopes from 121P1F1 as well as tumor-associated antigens that are often expressed with a target cancer associated with 121P1F1 expression, or can be administered as a composition comprising a cocktail of one or more discrete epitopes. Alternatively, the vaccine can be administered as a minigene construct or as dendritic cells which have been loaded with the peptide epitopes in vitro.

Example 26 Use of Peptides to Evaluate an Immune Response

[0640] Peptides of the invention may be used to analyze an immune response for the presence of specific antibodies, CTL or HTL directed to 121P1F1. Such an analysis can be performed in a manner described by Ogg et al., Science 279:2103-2106, 1998. In this Example, peptides in accordance with the invention are used as a reagent for diagnostic or prognostic purposes, not as an immunogen.

[0641] In this example highly sensitive human leukocyte antigen tetrameric complexes (“tetramers”) are used for a cross-sectional analysis of, for example, 121P1F1 HLA-A*0201-specific CTL frequencies from HLA A*02D 1-positive individuals at different stages of disease or following immunization comprising an 121P1F1 peptide containing an A*0201 motif. Tetrameric complexes are synthesized as described (Musey et al., N. Engl. J. Med. 337:1267, 1997). Briefly, purified HLA heavy chain (A*0201 in this example) and 2-microglobulin are synthesized by means of a prokaryotic expression system. The heavy chain is modified by deletion of the transmembrane-cytosolic tail and COOH-termiinal addition of a sequence containing a BirA enzymatic biotinylation site. The heavy chain, β2-microglobulin, and peptide are refolded by dilution. The 45-kD refolded product is isolated by fast protein liquid chromatography and then biotinylated by BirA in the presence of biotin (Sigma, St. Louis, Mo.), adenosine 5′ triphosphate and magnesium. Streptavidin-phycoerythrin conjugate is added in a 1:4 molar ratio, and the tetrameric product is concentrated to 1 mg/ml. The resulting product is referred to as tetramer-phycoerythrin.

[0642] For the analysis of patient blood samples, approximately one million PBMCs are centrifuged at 300 g for 5 minutes and resuspended in 50 μl of cold phosphate-buffered saline. Tri-color analysis is performed with the tetramer-phycoerythrin, along with anti-CD8-Tricolor, and anti-CD38. The PBMCs are incubated with tetramer and antibodies on ice for 30 to 60 min and then washed twice before formaldehyde fixation. Gates are applied to contain >99.98% of control samples. Controls for the tetramers include both A*0201-negative individuals and A*0201-positive non-diseased donors. The percentage of cells stained with the tetramer is then determined by flow cytometry. The results indicate the number of cells in the PBMC sample that contain epitope-restricted CTLs, thereby readily indicating the extent of immune response to the 121P1F1 epitope, and thus the status of exposure to 121P1F1, or exposure to a vaccine that elicits a protective or therapeutic response.

Example 27 Use of Peptide Epitopes to Evaluate Recall Responses

[0643] The peptide epitopes of the invention are used as reagents to evaluate T cell responses, such as acute or recall responses, in patients. Such an analysis may be performed on patients who have recovered from 121P1F1-associated disease or who have been vaccinated with an 121P1F1 vaccine.

[0644] For example, the class I restricted CTL response of persons who have been vaccinated may be analyzed. The vaccine may be any 121P1F1 vaccine. PBMC are collected from vaccinated individuals and HLA typed. Appropriate peptide epitopes of the invention that, optimally, bear supermotifs to provide cross-reactivity with multiple HLA supertype family members, are then used for analysis of samples derived from individuals who bear that HLA type.

[0645] PEBMC from vaccinated individuals are separated on Ficoll-Histopaque density gradients (Sigma Chemical Co., St. Louis, Mo.), washed three times in HBSS (GIBCO Laboratories), resuspended in RPMI-1640 (GIBCO Laboratories) supplemented with L-glutamine (2 mM), penicillin (50U/lm), streptomycin (50 μg/ml), and Hepes (10 mM) containing 10% heat-inactivated human AB serum (complete RPMI) and plated using microculture formats. A synthetic peptide comprising an epitope of the invention is added at 10 μg/mil to each well and HBV core 128-140 epitope is added at 1 μg/ml to each well as a source of T cell help during the first week of stimulation.

[0646] In the microculture format, 4×10⁵ PBMC are stimulated with peptide in 8 replicate cultures in 96-well round bottom plate in 100 μl/well of complete RPMI. On days 3 and 10, 100 μl of complete RPMI and 20 U/ml final concentration of rIL-2 are added to each well. On day 7 the cultures are transferred into a 96-well flat-bottom plate and restimulated with peptide, rIL-2 and 1 irradiated (3,000 rad) autologous feeder cells. The cultures are tested for cytotoxic activity on day 14. A positive CTL response requires two or more of the eight replicate cultures to display greater than 10% specific ⁵¹Cr release, based on comparison with non-diseased control subjects as previously described (Rehermann, et al., Nature Med. 2:1104,1108, 1996; Rehermann et al., J. Clin. Invest. 97:1655-1665, 1996; and Rehermann et al. J. Clin. Invest. 98:1432-1440, 1996).

[0647] Target cell lines are autologous and allogeneic EBV-transformed B-LCL that are either purchased from the American Society for Histocompatibility and Immunogenetics (ASHI, Boston, Mass.) or established from the pool of patients as described (Guilhot, et al. J. Virol. 66:2670-2678, 1992).

[0648] Ctytotoxicity assays are performed in the following manner. Target cells consist of either allogeneic HLA-matched or autologous EBV-transformed B lymphoblastoid cell line that are incubated overnight with the synthetic peptide epitope of the invention at 10 μM, and labeled with 100 μCi of ⁵¹Cr (Amersham Corp., Arlington Heights, Ill.) for 1 hour after which they are washed four times with HBSS.

[0649] Cytolytic activity is determined in a standard 4-h, split well ⁵¹Cr release assay using U-bottomed 96 well plates containing 3,000 targets/well. Stimulated PBMC are tested at effector/target (E/T) ratios of 20-50:1 on day 14. Percent cytotoxicity is determined from the formula: 100×[(experimental release-spontaneous release)/maximum release-spontaneous release)]. Maximum release is determined by lysis of targets by detergent (2% Triton X-100; Sigma Chemical Co., St. Louis, Mo.). Spontaneous release is <25% of maximum release for all experiments.

[0650] The results of such an analysis indicate the extent to which HLA-restricted CTL populations have been stimulated by previous exposure to 121P1F1 or an 121P1F1 vaccine.

[0651] Similarly, Class II restricted HTL responses may also be analyzed. Purified PBMC are cultured in a 96-well flat bottom plate at a density of 1.5×10⁵ cells/well and are stimulated with 10 μg/ml synthetic peptide of the invention, whole 121P1F1 antigen, or PHA. Cells are routinely plated in replicates of 4-6 wells for each condition. After seven days of culture, the medium is removed and replaced with fresh medium containing 10U/ml IL-2. Two days later, 1 μCi ³H-thymidine is added to each well and incubation is continued for an additional 18 hours. Cellular DNA is then harvested on glass fiber mats and analyzed for ³H-thymidine incorporation. Antigen-specific T cell proliferation is calculated as the ratio of ³H-thymidine incorporation in the presence of antigen divided by the ³H-thymidine incorporation in the absence of antigen.

Example 28 Induction of Specific CTL Response in Humans

[0652] A human clinical trial for an immunogenic composition comprising CTL and HTL epitopes of the invention is set up as an IND Phase I, dose escalation study and carried out as a randomized, double-blind, placebo-controlled trial. Such a trial is designed, for example, as follows:

[0653] A total of about 27 individuals are enrolled and divided into 3 groups:

[0654] Group I: 3 subjects are injected with placebo and 6 subjects are injected with 5 μg of peptide composition;

[0655] Group II: 3 subjects are injected with placebo and 6 subjects are injected with 50 μg peptide composition;

[0656] Group III: 3 subjects are injected with placebo and 6 subjects are injected with 500 μg of peptide composition.

[0657] Alter 4 weeks following the first injection, all subjects receive a booster inoculation at the same dosage.

[0658] The endpoints measured in this study relate to the safety and tolerability of the peptide composition as well as its immunogenicity. Cellular immune responses to the peptide composition are an index of the intrinsic activity of this the peptide composition, and can therefore be viewed as a measure of biological efficacy. The following summarize the clinical and laboratory data that relate to safety and efficacy endpoints.

[0659] Safety: The incidence of adverse events is monitored in the placebo and drug treatment group and assessed in terms of degree and reversibility.

[0660] Evaluation of Vaccine Efficacy: For evaluation of vaccine efficacy, subjects are bled before and after injection. Peripheral blood mononuclear cells are isolated from fresh heparinized blood by Ficoll-Hypaque density gradient centrifugation, aliquoted in freezing media and stored frozen. Samples are assayed for CTL and HTL activity.

[0661] The vaccine is found to be both safe and efficacious.

Example 29 Phase II Trials In Patients Expressing 121P1F1

[0662] Phase II trials are performed to study the effect of administering the CTL-HTL peptide compositions to patients having cancer that expresses 121P1F1. The main objectives of the trial are to determine an effective dose and regimen for inducing CTLs in cancer patients that express 121P1F1, to establish the safety of inducing a CTL and HTL response in these patients, and to see to what extent activation of CTLs improves the clinical picture of these patients, as manifested, e.g., by the reduction and/or shrinking of lesions. Such a study is designed, for example, as follows:

[0663] The studies are performed in multiple centers. The trial design is an open-label, uncontrolled, dose escalation protocol wherein the peptide composition is administered as a single dose followed six weeks later by a single booster shot of the same dose. The dosages are 50, 500 and 5,000 micrograms per injection. Drug-associated adverse effects (severity and reversibility) are recorded.

[0664] There are three patient groupings. The first group is injected with 50 micrograms of the peptide composition and the second and third groups with 500 and 5,000 micrograms of peptide composition, respectively. The patients within each group range in age from 21-65 and represent diverse ethnic backgrounds. All of them have a tumor that expresses 121P1F1.

[0665] Clinical manifestations or antigen-specific T-cell responses are monitored to assess the effects of administering the peptide compositions. The vaccine composition is found to be both safe and efficacious in the treatment of 121P1F1-associated disease.

Example 30 Induction of CTL Responses Using a Prime Boost Protocol

[0666] A prime boost protocol similar in its underlying principle to that used to confirm the efficacy of a DNA vaccine in transgenic mice, such as described above in the Example entitled “The Plasmid Construct and the Degree to Which It Induces Immunogenicity,” can also be used for the administration of the vaccine to humans. Such a vaccine regimen can include an initial administration of, for example, naked DNA followed by a boost using recombinant virus encoding the vaccine, or recombinant protein/polypeptide or a peptide mixture administered in an adjuvant.

[0667] For example, the initial immunization may be performed using an expression vector, such as that constructed in the Example entitled “Construction of ‘Minigene’ Multi-Epitope DNA Plasmids” in the form of naked nucleic acid administered IM (or SC or ID) in the amounts of 0.5-5 mg at multiple sites. The nucleic acid (0.1 to 1000 μg) can also be administered using a gene gun. Following an incubation period of 3-4 weeks, a booster dose is then administered. The booster can be recombinant fowlpox virus administered at a dose of 5-10⁷ to 5×10⁹ pfu. An alternative recombinant virus, such as an MVA, canarypox, adenovirus, or adeno-associated virus, can also be used for the booster, or the polyepitopic protein or a mixture of the peptides can be administered. For evaluation of vaccine efficacy, patient blood samples are obtained before immunization as well as at intervals following administration of the initial vaccine and booster doses of the vaccine. Peripheral blood mononuclear cells are isolated from fresh heparinized blood by Ficoll-Hypaque density gradient centrifugation, aliquoted in freezing media and stored frozen. Samples are assayed for CTL and HTL activity.

[0668] Analysis of the results indicates that a magnitude of response sufficient to achieve a therapeutic or protective immunity against 121P1F1 is generated.

Example 31 Administration of Vaccine Compositions Using Dendritic Cells (DC)

[0669] Vaccines comprising peptide epitopes of the invention can be administered using APCs, or “professional” APCs such as DC. In this example, peptide-pulsed DC are administered to a patient to stimulate a CTL response in vivo. In this method, dendritic cells are isolated, expanded, and pulsed with a vaccine comprising peptide CTL and HTL epitopes of the invention. The dendritic cells are infused back into the patient to elicit CTL and HTL responses in vivo. The induced CTL and HTL then destroy or facilitate destruction, respectively, of the target cells that bear the 121P1F1 protein from which the epitopes in the vaccine are derived.

[0670] For example, a cocktail of epitope-comprising peptides is administered ex vivo to PBMC, or isolated DC therefrom. A pharmaceutical to facilitate harvesting of DC can be used, such as Progenipoietin™ (Monsanto, St. Louis, Mo.) or GM-CSF/IL-4. After pulsing the DC with peptides, and prior to reinfusion into patients, the DC are washed to remove unbound peptides.

[0671] As appreciated clinically, and readily determined by one of skill based on clinical outcomes, the number of DC reinfused into the patient can vary (see, e.g., Nature Med. 4:328, 1998; Nature Med. 2:52, 1996 and Prostate 32:272, 1997). Although 2-50×10⁶ DC per patient are typically administered, larger number of DC, such as 10⁷ or 10⁸ can also be provided. Such cell populations typically contain between 50-90% DC.

[0672] In some embodiments, peptide-loaded PBMC are injected into patients without purification of the DC. For example, PBMC generated after treatment with an agent such as Progenipoietin™ are injected into patients without purification of the DC. The total number of PBMC that are administered often ranges from 10⁸ to 10¹⁰. Generally, the cell doses injected into patients is based on the percentage of DC in the blood of each patient, as determined, for example, by immunofluorescence analysis with specific anti-DC antibodies. Thus, for example, if Progenipoietin™ mobilizes 2% DC in the peripheral blood of a given patient, and that patient is to receive 5×10⁶ DC, then the patient will be injected with a total of 2.5×10⁸ peptide-loaded PBMC. The percent DC mobilized by an agent such as Progenipoietin™ is typically estimated to be between 2-10%, but can vary as appreciated by one of skill in the art.

[0673] Ex Vivo Activation of CTL/HTL Responses

[0674] Alternatively, ex vivo CTL or HTL responses to 121P1F1 antigens can be induced by incubating, in tissue culture, the patient's, or genetically compatible, CTL or HTL precursor cells together with a source of APC, such as DC, and immunogenic peptides. After an appropriate incubation time (typically about 7-28 days), in which the precursor cells are activated and expanded into effector cells, the cells are infused into the patient, where they will destroy (CTL) or facilitate destruction (HTL) of their specific target cells, i.e., tumor cells.

Example 32 An Alternative Method of Identifying and Confirming Motif-Bearing Peptides

[0675] Another method of identifying and confirming motif-bearing peptides is to elute them from cells bearing defined MHC molecules. For example, EBV transformed B cell lines used for tissue typing have been extensively characterized to determine which HLA molecules they express. In certain cases these cells express only a single type of HLA molecule. These cells can be transfected with nucleic acids that express the antigen of interest, e.g. 121P1F1. Peptides produced by endogenous antigen processing of peptides produced as a result of transfection will then bind to HLA molecules within the cell and be transported and displayed on the cell's surface. Peptides are then eluted from the HLA molecules by exposure to mild acid conditions and their amino acid sequence determined, e.g., by mass spectral analysis (e.g., Kubo et al., J. Immunol. 152:3913, 1994). Because the majority of peptides that bind a particular HLA molecule are motif-bearing, this is an alternative modality for obtaining the motif-bearing peptides correlated with the particular HLA molecule expressed on the cell.

[0676] Alternatively, cell lines that do not express endogenous HLA molecules can be transfected with an expression construct encoding a single HLA allele. These cells can then be used as described, i.e., they can then be transfected with nucleic acids that encode 121P1F1 to isolate peptides corresponding to 121P1F1 that have been presented on the cell surface. Peptides obtained from such an analysis will bear motif(s) that correspond to binding to the single HLA allele that is expressed in the cell.

[0677] As appreciated by one in the art, one can perform a similar analysis on a cell bearing more than one HLA allele and subsequently determine peptides specific for each HLA allele expressed. Moreover, one of skill would also recognize that means other than transfection, such as loading with a protein antigen, can be used to provide a source of antigen to the cell.

Example 33 Complementary Polynucleotides

[0678] Sequences complementary to the 121P1F1-encoding sequences, or any parts thereof, are used to detect, decrease, or inhibit expression of naturally occurring 121P1F1. Although use of oligonucleotides comprising from about 15 to 30 base pairs is described, essentially the same procedure is used with smaller or with larger sequence fragments. Appropriate oligonucleotides are designed using, e.g., OLIGO 4.06 software (Stational Biosciences) and the coding sequence of 121P1F1. To inhibit transcription, a complementary oligonucleotide is designed from the most unique 5′ sequence and used to prevent promoter binding to the coding sequence. To inhibit translation, a complementary oligonucleotide is designed to prevent ribosomal binding to a 121P1F1-encoding transcript.

Example 34 Purification of Naturally-Occurring or Recombinant 121P1F1 Using 121P1F1 Specific Antibodies

[0679] Naturally occurring or recombinant 121P1F 1 is substantially purified by immunoaffinity chromatography using antibodies specific for 121P1F1. An immunoaffinity column is constructed by covalently coupling anti-121P1F 1 antibody to an activated chromatographic resin, such as CNBr-activated SEPHAROSE (Amersham Pharmacia Biotech). After the coupling, the resin is blocked and washed according to the manufacturer's instructions.

[0680] Media containing 121P1F1 are passed over the immunoaffinity column, and the column is washed under conditions that allow the preferential absorbance of 121P1F1 (e.g., high ionic strength buffers in the presence of detergent). The column is eluted under conditions that disrupt antibody/121P1F1 binding (e.g., a buffer of pH 2 to pH 3, or a high concentration of a chaotrope, such as urea or thiocyanate ion), and GCR.P is collected.

Example 35 Identification of Molecules Which Interact with 121P1F1

[0681] 121P1F1, or biologically active fragments thereof, are labeled with 121 1 Bolton-Hunter reagent. (See, e.g., Bolton et at (1973) Biochem. J. 133:529.) Candidate molecules previously arrayed in the wells of a multi-well plate are incubated with the labeled 121P1F1, washed, and any wells with labeled 121P1F1 complex are assayed. Data obtained using different concentrations of 121P1F1 are used to calculate values for the number, affinity, and association of 121P1F1 with the candidate molecules.

Example 36 In Vivo Assay for 121P1F1 Tumor Growth Promotion

[0682] The effect of the 121P1F1 protein on tumor cell growth is evaluated in vivo by evaluating tumor development and growth of cells expressing or lacking 121P1F1. For example, SCID mice are injected subcutaneously on each flank with 1×10⁶ of either 3T3, prostate, kidney or breast cancer cell lines (e.g. PC3, DU145, CaKi, SW 839, MCF7 cells) containing tkNeo empty vector or 121P1F1. At least two strategies can be used: (1) Constitutive 121P1F1 expression under regulation of a promoter, such as a constitutive promoter obtained from the genomes of viruses such as polyorra virus, fowlpox virus (see UK 2,211,504, published Jul. 5, 1989), adenovirus (such as Adenovirus 2), bovine papilloma virus, avian sarcoma virus, cytomegalovirus, a retrovirus, hepatitis-B virus and Simian Virus 40 (SV40), or from heterologous mammalian promoters, e.g., the actin promoter or an immunoglobulin promoter, provided such promoters are compatible with the host cell systems, and (2) Regulated expression under control of an inducible vector system, such as ecdysone, tetracycline, etc., provided such promoters are compatible with the host cell systems. Tumor volume is then monitored by caliper measurement at the appearance of palpable tumors and followed over time to determine if 121P1F1-expressing cells grow at a faster rate and whether tumors produced by 121P1F1-expressing cells demonstrate characteristics of altered aggressiveness (e.g. enhanced metastasis, vascularization, reduced responsiveness to chemotherapeutic drugs).

[0683] Additionally, mice can be implanted with 1×10⁵ of the same cells orthotopically to determine if 121P1F1 has an effect on local growth in the prostate, kidney or mammary gland, and whether 121P1F1 affects the ability of the cells to metastasize, specifically to lungs, lymph nodes, and bone marrow.

[0684] The assay is also useful to determine the 121P1F1 inhibitory effect of candidate therapeutic compositions, such as for example, 121P1F1 intrabodies, 121P1F1 antisense molecules and ribozymes.

Example 37: 121P1F1 Monoclonal Antibody-Mediated Inhibition of Prostate and Kidney Tumors In Vivo

[0685] The significant expression of 121P1F1 in cancer tissues, together with its restrictive expression in normal tissues, makes 121P1F1 a good target for antibody therapy. Similarly, 121P1F1 is a target for T cell-based immunotherapy. Thus, the therapeutic efficacy of anti-121P1F1 mAbs in human prostate cancer xenograft mouse models is evaluated by using androgen-independent LAPC4 and LAPC-9 xenografts (Craft, N., et al., Cancer Res, 1999. 59(19): p. 5030-6) the androgen independent recombinant cell line PC3-121P1F1 and 3T3-121P1F1 (see, e.g., Kaighn, M. E., et al., Invest Urol, 1979. 17(1): p. 16-23). Similarly, anti-121P1F mAbs are evaluated in human kidney cancer xenograft models such as AGS-K3 and AGS-K.6 and in recombinant kidney cell lines such as CaKi-121P1F1.

[0686] Antibody efficacy on tumor growth and metastasis formation is studied, e.g., in a mouse orthotopic prostate cancer xenograft models and mouse kidney xenograft models. The antibodies can be unconjugated, as discussed in this Example, or can be conjugated to a therapeutic modality, as appreciated in the art. Anti-121P1F 1 mAbs inhibit formation of both the androgen-dependent LAPC-9 and androgen-independent PC3-121P1F1 tumor xenografts. Anti-121P1F1 mAbs also retard the growth of established orthotopic tumors and prolonged survival of tumor-bearing mice. These results indicate the utility of anti-121P1F 1 mAbs in the treatment of local and advanced stages of prostate cancer. (See, e.g., Saffran, D., et al., PNAS 10:1073-1078 or www.pnas.org/cgi/doi/10.1073/nnas.051624698). Similarly, anti-121P1F 1 mAbs can inhibit formation of AGS-K3 and AGS-K6 tumors in SCID mice, and prevent or retard the growth of CaKi-121P1F1 tumor xenografts. These results indicate utility of anti-121P1F1 mAbs for treatment of kidney cancer.

[0687] Administration of the anti-121P1F1 mAbs leads to retardation of established orthotopic tumor growth and inhibition of metastasis to distant sites, resulting in a significant prolongation in the survival of tumor-bearing mice. These studies indicate that 121P1F1 as an attractive target for immunotherapy and demonstrate the therapeutic potential of anti-121P1F1 mAbs for the treatment of local and metastatic prostate cancer. This example demonstrates that unconjugated 121P1F1 monoclonal antibodies are effective to inhibit the growth of human prostate tumor xenografts and human kidney xenografts grown in SCID mice; accordingly a combination of such efficacious monoclonal antibodies is also effective.

[0688] Tumor Inhibition using Multiple Unconjugated 121P1F1 mAbs Materials and Methods

[0689] 12 IP1F1 Monoclonal Antibodies:

[0690] Monoclonal antibodies are raised against 121P1F1 as described in the Example entitled “Generation of 121P1F1 Monoclonal Antibodies (mAbs).” The antibodies are characterized by ELISA, Western blot, FACS, and immunoprecipitation for their capacity to bind 121P1F1. Epitope mapping data for the anti-121P1F1 mAbs, as determined by ELISA and Western analysis, recognize epitopes on the 121P1F1 protein. Immunohistochemical analysis of prostate cancer tissues and cells with these antibodies is performed.

[0691] The monoclonal antibodies are purified from ascites or hybridoma tissue culture supernatants by Protein-G Sepharose chromatography, dialyzed against PBS, filter sterilized, and stored at −20° C. Protein determinations are performed by a Bradford assay (Bio-Rad, Hercules, Calif.). A therapeutic monoclonal antibody or a cocktail comprising a mixture of individual monoclonal antibodies is prepared and used for the treatment of mice receiving subcutaneous or orthotopic injections of LAPC-9 prostate tumor xenografts.

[0692] Cancer Xenografts and Cell Lines

[0693] The LAPC-9 xenograft, which expresses a wild-type androgen receptor and produces prostate-specific antigen (PSA), is passaged in 6- to 8-week-old male ICR-severe combined immunodeficient (SCID) mice (Taconic Farms) by s.c. trocar implant (Craft, N., et al., supra). The AGS-K3 and AGS-K6 kidney xenografts are also passaged by subcutaneous implants in 6- to 8-week old SCID mice. Single-cell suspensions of tumor cells are prepared as described in Craft, et al. The prostate carcinoma cell line PC3 (American Type Culture Collection) is maintained in RPMI supplemented with L-glutamine and 10% FBS, and the kidney carcinoma line CaKi as well as N1H-3T3 cells (American Type Culture Collection) are maintained in DMEM supplemented with L-glutamine and 10% FBS.

[0694] A PC3-121P1F1, CaKi-121P1F1 and 3T3-121P1F1 cell populations are generated by retroviral gene transfer as described in Hubert, R. S., et al., STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors. Proc Natl Acad Sci USA, 1999. 96(25): p. 14523-8.

[0695] Xtenograft Mouse Models.

[0696] Subcutaneous (s.c.) tumors are generated by injection of 1×10⁶ LAPC-9, AGS-K3, AGS-K6, PC3, PC3-121P1F1, CaKi or CaKi-121P1F1 cells mixed at a 1:1 dilution with Matrigel (Collaborative Research) in the right flank of male SCID mice. To test antibody efficacy on tumor formation, i.p. antibody injections are started on the same day as tumor-cell injections. As a control, mice are injected with either purified mouse IgG (ICN) or PBS; or a purified monoclonal antibody that recognizes an irrelevant antigen not expressed in human cells. In preliminary studies, no difference is found between mouse IgG or PBS on tumor growth. Tumor sizes are determined by vernier caliper measurements, and the tumor volume is calculated as length×width×height. Mice with s.c. tumors greater than 1.5 cm in diameter are sacrificed. PSA levels are determined by using a PSA ELISA kit (Anogen, Mississauga, Ontario). Circulating levels of anti-121P1F1 mAbs are determined by a capture ELISA kit (Bethyl Laboratories, Montgomery, Tex.). (See, e.g., (Saffran, D., et al., PNAS 10: 1073-1078 or www.pnas.org/cgi/doi/10.1073/pnas.051624698)

[0697] Orihotopic injections are performed under anesthesia by using ketamine/xylazine. For prostate orthotopic studies, an incision is made through the abdominal muscles to expose the bladder and seminal vesicles, which then are delivered through the incision to expose the dorsal prostate. LAPC-9 cells (5×10⁵) mixed with Matrigel are injected into each dorsal lobe in a 10-μl volume. To monitor tumor growth, mice are bled on a weekly basis for determination of PSA levels. For kidney orthotopic models, an incision is made through the abdominal muscles to expose the kidney. AGS-K3 or AGS-K6 cells mixed with Matrigel are injected under the kidney capsule. The mice are segregated into groups for the appropriate treatments, with anti-121P1F1 or control mAbs being injected i.p.

[0698] Anti-121P1F1 mAbs Inhibit Growth of 121P1F1-Expressing Xenograft-Cancer Tumors

[0699] The effect of anti-121P1F1 mAbs on tumor formation is tested by using LAPC-9 and AGS-K3 orthotopic models. As compared with the s.c. tumor model, the orthotopic model, which requires injection of tumor cells directly in the mouse prostate or kidney, respectively, results in local tumor growth, development of metastasis in distal sites, deterioration of mouse health, and subsequent death (Saffran, D., et al., PNAS supra; Fu, X., et al., Int J Cancer, 1992. 52(6): p. 987-90; Kubota, T., J Cell Biochem, 1994. 56(1): p. 4-8). The features make the orthotopic model more representative of human disease progression and allow the therapeutic effect of mAbs on clinically relevant end points to be followed.

[0700] Accordingly, tumor cells are injected into the mouse prostate or kidney, and 2 days later, the mice are segregated into two groups and treated with either: a) 200-500 μg of anti-121P1F1 Ab, or b) PBS three times per week for two to five weeks.

[0701] A major advantage of the orthotopic prostate-cancer model is the ability to study the development of metastases. Formation of metastasis in mice bearing established orthotopic tumors is studied by IHC analysis on lung sections using an antibody against a prostate-specific cell-surface protein STEAP expressed at high levels in LAPC-9 xenografts (Hubert, R. S., et al., Proc Natl Acad Sci USA, 1999. 96(25): p. 14523-8) or anti-G250 antibody for kidney cancer models.

[0702] Mice bearing established orthotopic LAPC-9 tumors are administered 1000 μg injections of either anti-121P1F1 mAb or PBS over a 4-week period. Mice in both groups are allowed to establish a high tumor burden (PSA levels greater than 300 ng/ml), to ensure a high frequency of metastasis formation in mouse lungs. Mice then are killed and their prostate/kideny and lungs are analyzed for the presence of tumor cells by IHC analysis.

[0703] These studies demonstrate a broad anti-tumor efficacy of anti-121P1F1 antibodies on initiation and progression of prostate and kidney cancer in xenograft mouse models. Anti-121P1F1 antibodies inhibit tumor formation of both androgen-dependent and androgen-independent tumors, retard the growth of already established tumors, and prolong the survival of treated mice. Moreover, anti-121P1F1 mAbs demonstrate a dramatic inhibitory effect on the spread of local prostate tumor to distal sites, even in the presence of a large tumor burden. Thus, anti-121P1F1 mAbs are efficacious on major clinically relevant end points (tumor growth), prolongation of survival, and health.

Example 38 Therapeutic and Diagnostic use of Anti-121P1F1 Antibodies in Humans

[0704] Anti-121P1F1 monoclonal antibodies are safely and effectively used for diagnostic, prophylactic, prognostic and/or therapeutic purposes in humans. Western blot and immunohistochemical analysis of cancer tissues and cancer xenografts with anti-121P1F1 mAb show strong extensive staining in carcinoma but significantly lower or undetectable levels in normal tissues. Detection of 121P1F1 in carcinoma and in metastatic disease demonstrates the usefulness of the mAb as a diagnostic and/or prognostic indicator. Anti-121P1F1 antibodies are therefore used in diagnostic applications such as immunohistochemistry of kidney biopsy specimens to detect cancer from suspect patients.

[0705] As determined by flow cytometry, anti-121P1F1 mAb specifically binds to carcinoma cells. Thus, anti-121P1F1 antibodies are used in diagnostic whole body imaging applications, such as radioimmunoscintigraphy and radioimmunotherapy, (see, e.g., Potamianos S., et. al. Anticancer Res 20(2A):925.-948 (2000)) for the detection of localized and metastatic cancers that exhibit expression of 121P1F1. Shedding or release of an extracellular domain of 121P1F1 into the extracellular milieu, such as that seen for alkaline phosphodiesterase B10 (Meerson, N. R., Hepatology 27:563-568 (1998)), allows diagnostic detection of 121P1F1 by anti-121P1F1 antibodies in serum and/or urine samples from suspect patients.

[0706] Anti-121P1F1 antibodies that specifically bind 121P1F 1 are used in therapeutic applications for the treatment of cancers that express 121P1F1. Anti-121P1F1 antibodies are used as an unconjugated *modality and as conjugated form in which the antibodies are attached to one of various therapeutic or imaging modalities well known in the art, such as a prodrugs, enzymes or radioisotopes. In preclinical studies, unconjugated and conjugated anti-121P1F1 antibodies are tested for efficacy of tumor prevention and growth inhibition in the SCID mouse cancer xenograft models, e.g., kidney cancer models AGS-K3 and AGS-K6, (see, e.g., the Example entitled “Monoclonal Antibody-mediated Inhibition of Prostate and Kidney Tumors In vivo.” Conjugated and unconjugated anti-121P1F1 antibodies are used as a therapeutic modality in human clinical trials either alone or in combination with other treatments as described in following Examples.

Example 39 Human Clinical Trials for the Treatment and Diagnosis of Human Carcinomas through use of Human Anti-121P1F1 Antibodies In vivo

[0707] Antibodies are used in accordance with the present invention which recognize an epitope on 121P1F1, and are used in the treatment of certain tumors such as those listedin Table I. Based upon a number of factors, including 121P1F1 expression levels, tumors such as those listed in Table I are presently preferred indications. In connection with each of these indications, three clinical approaches are successfully pursued.

[0708] I.) Adjunctive therapy: In adjunctive therapy, patients are treated with anti-121P1F1 antibodies in combination with a chemotherapeutic or antineoplastic agent and/or radiation therapy. Primary cancer targets, such as those listed in Table I, are treated under standard protocols by the addition anti-121P1F1 antibodies to standard first and second line therapy. Protocol designs address effectiveness as assessed by reduction in tumor mass as well as the ability to reduce usual doses of standard chemotherapy. These dosage reductions allow additional and/or prolonged therapy by reducing dose-related toxicity of the chemotherapeutic agent. Anti-121P1F1 antibodies are utilized in several adjunctive clinical trials in combination with the chemotherapeutic or antineoplastic agents adriamycin (advanced prostrate carcinoma), cisplatin (advanced head and neck and lung carcinomas), taxol (breast cancer), and doxorubicin (preclinical).

[0709] II.) Monotherapy: In connection with the use of the anti-121P1F1 antibodies in monotherapy of tumors, the antibodies are administered to patients without a chemotherapeutic or antineoplastic agent. In one embodiment, monotherapy is conducted clinically in end stage cancer patients with extensive metastatic disease. Patients show some disease stabilization. Trials demonstrate an effect in refractory patients with cancerous tumors.

[0710] III.) Imaging Agent: Through binding a radionuclide (e.g., iodine or yttrium (I¹³¹, Y⁹⁰) to anti-121P1F1 antibodies, the radiolabeled antibodies are utilized as a diagnostic and/or imaging agent. In such a role, the labeled antibodies localize to both solid tumors, as well as, metastatic lesions of cells expressing 121P1F1. In connection with the use of the anti-121P1F1 antibodies as imaging agents, the antibodies are used as an adjunct to surgical treatment of solid tumors, as both a pre-surgical screen as well as a post-operative follow-up to determine what tumor remains and/or returns. In one embodiment, a (¹¹¹In)-121P1F1 antibody is used as an imaging agent in a Phase I human clinical trial in patients having a carcinoma that expresses 121P1F1 (by analogy see, e.g., Divgi et al. J. Natl. Cancer Inst. 83:97-104 (1991)). Patients are followed with standard anterior and posterior gamma camera. The results indicate that primary lesions and metastatic lesions are identified

[0711] Dose and Route of Administration

[0712] As appreciated by those of ordinary skill in the art, dosing considerations can be determined through comparison with the analogous products that are in the clinic. Thus, anti-121P1F1 antibodies can be administered with doses in the range of 5 to 400 mg/m ², with the lower doses used, e.g., in connection with safety studies. The affinity of anti-121P1F1 antibodies relative to the affinity of a known antibody for its target is one parameter used by those of skill in the art for determining analogous dose regimens. Further, anti-121P1F1 antibodies that are fully human antibodies, as compared to the chimeric antibody, have slower clearance; accordingly, dosing in patients with such fully human anti-121P1F 1 antibodies can be lower, perhaps in the range of 50 to 300 mg/m², and still remain efficacious. Dosing in mg/m², as opposed to the conventional measurement of dose in mg/kg, is a measurement based on surface area and is a convenient dosing measurement that is designed to include patients of all sizes from infants to adults.

[0713] Three distinct delivery approaches are useful for delivery of anti-121P1F1 antibodies. Conventional intravenous delivery is one standard delivery technique for many tumors. However, in connection with tumors in the peritoneal cavity, such as tumors of the ovaries, biliary duct, other ducts, and the like, intraperitoneal administration may prove favorable for obtaining high dose of antibody at the tumor and t) also minimize antibody clearance. In a similar manner, certain solid tumors possess vasculature that is appropriate for regional perfusion. Regional perfusion allows for a high dose of antibody at the site of a tumor and minimizes short term clearance of the antibody.

[0714] Clinical Development Plan (CDP)

[0715] Overview: The CDP follows and develops treatments of anti-121P1F1 antibodies in connection with adjunctive therapy, monotherapy, and as an imaging agent. Trials initially demonstrate safety and thereafter confirm efficacy in repeat doses. Trails are open label comparing standard chemotherapy with standard therapy plus anti-121P1F1 antibodies. As will be appreciated, one criteria that can be utilized in connection with enrollment of patients is 121P1F1 expression levels in their tumors as determined by biopsy.

[0716] As with any protein or antibody infusion-based therapeutic, safety concerns are related primarily to (i) cytokine release syndrome, i.e., hypotension, fever, shaking, chills; (ii) the development of an immunogenic response to the material (i.e., development of human antibodies by the patient to the antibody therapeutic, or HAHA response); and, (iii) toxicity to normal cells that express 121P1F1. Standard tests and follow-up are utilized to monitor each of these safety concerns. Anti-121P1F1 antibodies are found to be safe upon human administration.

Example 40 Human Clinical Trial Adjunctive Therapy with Human Anti-121P1F1 Antibody and Chemotherapeutic Agent

[0717] A phase I human clinical trial is initiated to assess the safety of six intravenous doses of a human anti-121P1F1 antibody in connection with the treatment of a solid tumor, e.g., a cancer of a tissue listed in Table I. In the study, the safety of single doses of anti-121P1F1 antibodies when utilized as an adjunctive therapy to an antineoplastic or chemotherapeutic agent, such as cisplatin, topotecan, doxorubicin, adriamycin, taxol, or the like, is assessed. The trial design includes delivery of six single doses of an anti-121P1F1 antibody with dosage of antibody escalating from approximately about 25 mg/m² to about 275 mg/m² over the course of the treatment in accordance with the following schedule: Day 0 Day 7 Day 14 Day 21 Day 28 Day 35 mAb Dose 25 75 125 175 225 275 mg/m² mg/m² mg/m² mg/m² mg/m² mg/m² Chemotherapy + + + + + + (standard dose)

[0718] Patients are closely followed for one-week following each administration of antibody and chemotherapy. In particular, patients are assessed for the safety concerns mentioned above: (i) cytokine release syndrome, i.e., hypotension, fever, shaking, chills; (ii) the development of an immunogenic response to the material (i.e., development of human antibodies by the patient to the human antibody therapeutic, or HAHA response); and, (iii) toxicity to normal cells that express 121P1F1. Standard tests and follow-up are utilized to monitor each of these safety concerns. Patients are also assessed for clinical outcome, and particularly reduction in tumor mass as evidenced by MRI or other imaging.

[0719] The anti-121P1F1 antibodies are demonstrated to be safe and efficacious, Phase II trials confirm the efficacy and refine optimum dosing.

Example 41 Human Clinical Trial: Monotherapy with Human Anti-121P1F1 Antibody

[0720] Anti-121P1F1 antibodies are safe in connection with the above-discussed adjunctive trial, a Phase II human clinical trial confirms the efficacy and optimum dosing for monotherapy. Such trial is accomplished, and entails the same safety and outcome analyses, to the above-described adjunctive trial with the exception being that patients do not receive chemotherapy concurrently with the receipt of doses of anti-121P1F1 antibodies.

Example 42 Human Clinical Trial: Diagnostic Imaging with Anti-121P1F1 Antibody

[0721] Once again, as the adjunctive therapy discussed above is safe within the safety criteria discussed above, a human clinical trial is conducted concerning the use of anti-121P1F1 antibodies as a diagnostic imaging agent. The protocol is designed in a substantially similar manner to those described in the art, such as in Divgi et al. J. Natl. Cancer Inst. 83:97-104 (1991). The antibodies are found to be both safe and efficacious when used as a diagnostic modality.

Example 43 Homology Comparison of 121P1F1 to Known Sequences

[0722] The 121P1F1 gene is identical to a previously cloned and sequenced gene, namely human GAJ protein (gi|14149769) showing 100% identity to that protein. The closest homolog to the 121P2F1 protein is a mouse hypothetical 24.2 kDa protein (gi|12847934) of unknown function. The 121P1F1 protein consists of 205 amino acids, with calculated molecular weight of 23.7 kDa, and pI of 8.2. 121P1F1 is an intracellular protein, with primary localization to the nucleus. 121P1F1 can also localize to the cytosol. Motif analysis revealed the presence of a basic leucine zipper motif(bZIP) (Table XXI) in 121P1F1 at amino acids 117-143, and a steroid hormone receptor signature at aa 168-189. The basic-leucine zipper (bZIP) (Table XXI) motif mediates sequence-specific DNA-binding and dimerization of leucine zipper motifs withi other basic helix-loop-helix proteins (Alber T. Curr Opin Genet Dev. 1992, 2:205). This dimerization of the transcription factor is critical in order for DNA binding and transcriptional activation to occur. Members of the leucine zipper family of proteins include the Myc proto-oncogene (Amati B,et al. EMBO J. 1993, 12:5083). The Myc-Max dimer is a transactivating complex which regulates the expression of various genes, including genes involved in cell proliferation, growth and apoptosis, as well as differentiation (Luscher B. Gene. 2001, 277:1; Holzel M, et al, EMBO Rep. 2001, 2:1125; Ben-Porath I, Yanuka O, Benvenisty N. Mol Cell Biol. 1999, 19:3529). Myc is overexpressed in a variety of cancers, including prostate, breast and colon cancer (Jenkins R B, Qian J, Lieber M M, Bostwick D G. Cancer Res. 1997, 57:524; Buttyan R, et al. Prostate. 1987; 11:327; Chrzan P, et al. Clin Biochem. 2001, 34:557; Hashimoto K et al, Carcinogenesis. 2001, 22:1965). The steroid hormone receptor signature is a fingerprint with similarity to the zinc finger motif. It is often found in transcription factors, where it regulates DNA-protein and protein-protein interactions by determining the specificity of interacting partners (Green S et al, EMBO J. 1988,7:3037; Ribeiro R C, Kushner P J, Baxter J D. Annu Rev Med. 1995;46:443).

[0723] The presence of leucine zipper and protein-protein interaction domains along with its localization to the nucleus indicate that 121P1F1 plays a role in regulating gene transcription in mammalian cells, and thereby regulates cellular proliferation, transformation, differentiation and apoptosis. These biological functions have a direct effect on transformation, tumor growth and progression.

[0724] Accordingly, when 121P1F1 functions as a regulator of cell transformation, tumor formation, or as a modulator of transcription involved in activating genes associated with inflammation, tumorigenesis or proliferation, 121P1F1 is useful for therapeutic, diagnostic, prognostic and/or preventative purposes. In addition, when a molecule, such as a variant or SNP of 121P1F1, is expressed in cancerous tissues, such as those listed in Table I, it is useful for therapeutic, diagnostic, prognostic and/or preventative purposes.

[0725] Several variants of 121P1F1 have been identified, including the 5 variants shown in FIG. 10 and FIG. 11. Several of the variants (e.g. V1A, V2, V3 and V4) contain portions of 121P1F1 while lacking others. Other variants contain additional sequences not found in 121P1F1 (e.g. V1A, V2 and V3). For example, variant 1A is identical to 121P1F1 in its first 92 aa, while lacking aa 93-205 of 121P1F1 and diverging from 121P1F1in its C-terminal 34 aa (FIG. 4A and FIG. 4B). Variants 1B, 3 and 4 contain a Myc-like leucine zipper, indicating that they bind DNA and function as transcription factors in a manner similar to full length 121P1F1. Properties of 121P1F1 and splice variants 1A and 4 are shown in Table XXI.

Example 44 Regulation of Transcription

[0726] The nuclear localization of 121P1F1 coupled to the presence of bZIP and protein interaction domains within its sequence indicate that 121P1F1 is a transcription factor and modulates the transcriptional regulation of eukaryotic genes. This function is supported by published reports, which show that Myc regulates the expression of multiple genes including Tmp, a gene that promotes transformation (Ben-Porath. I, Yanuka O, Benvenisty N. Mol Cell Biol. 1999,19:3529), and p21WAF1, a gene that controls the cell cycle (Mitchell K O and El-Deiry W S, Cell Growth Differ 1999, 10:223). Regulation of gene expression is confirmed, e.g., by studying gene expression in cells expressing or lacking 121P1F1. For this purpose, two types of experiments are performed.

[0727] In the first set of experiments, RNA from parental and 121P1F1-expressing cells are extracted and hybridized to commercially available gene arrays (Clontech) (Smid-Koopman E et al. Br J Cancer. 2000. 83:246). Resting cells as well as cells treated with FBS, androgen or growth factors are compared. Differentially expressed genes are identified in accordance with procedures known in the art. The differentially expressed genes are then mapped to biological pathways (Chen K et al. Thyroid. 2001. 11:41.).

[0728] In the second set of experiments, specific transcriptional pathway activation is evaluated using commercially available (Stratagene) luciferase reporter constructs including: NFκB-luc, SRE-luc, ELK1-luc, ARE-luc, p53-luc, and CRE-luc. In addition, a Myc/Max specific response element, namely E-box hexamer CACGTG reporter is also evaluated (Ben-Porath I et al, Mol Cell Biol 1999;19:3529). These transcriptional reporters contain consensus binding sites for known transcription factors that lie downstream of well-characterized signal transduction pathways, and represent a good tool to ascertain pathway activation and screen for positive and negative modulators of pathway activation.

[0729] Thus, 121P1F1 plays a role in gene regulation, and it is used as a target for diagnostic, prognostic, preventative and/or therapeutic purposes.

Example 45 Identification and Confirmation of Potential Signal Transduction Pathways

[0730] Many mammalian proteins have been reported to interact with signaling molecules and to participate in regulating signaling pathways. (J. Neurochem. 2001; 76:217-223). Based on their ability to mediate protein interactions, leucine zipper proteins have been reported to regulate signaling pathways important for cell survival and growth (Nagamura-Inoue T et al, Int Rev Immunol. 2001, 20:83). Using immunoprecipitation and Western blotting techniques, proteins are identified that associate with 121P1F1 and mediate signaling events. Several pathways known to play a role in cancer biology can be regulated by 121P1F1, including phospholipid pathways such as PI3K, AKT, etc, adhesion and migration pathways, including FAK, Rho, Rac-1, etc, as well as mitogenic/survival cascades such as ERK, p38, etc (Cell Growth Differ. 2000, 11:279; J Biol Chem. 1999,274:801; Oncogene. 2000, 19:3003, J. Cell Biol. 1997, 138:913.).

[0731] To confirm that 121P1F1 directly or indirectly activates known signal transduction pathways in cells, luciferase (luc) based transcriptional reporter assays are carried out in cells expressing individual genes. These transcriptional reporters contain consensus-binding sites for known transcription factors that lie downstream of well-characterized signal transduction pathways. The reporters and examples of these associated transcription factors, signal transduction pathways, and activation stimuli are listed below.

[0732] 1. NFκB-luc, NFκB/Rel; Ik-kinase/SAPK; growth/apoptosis/stress

[0733] 2. SRE-luc, SRF/TCF/ELK1; MAPK/SAPK; growth/differentiation

[0734] 3. AP-1-luc, FOS/JUN; MAPK/SAPK/PKC; growth/apoptosis/stress

[0735] 4. ARE-luc, androgen receptor; steroids/MAPK; growth/differentiation/apoptosis

[0736] 5. p53-luc, p53; SAPK; growth/differentiation/apoptosis

[0737] 6. CRE-luc, CREB/ATF2; PKA/p38; growth/apoptosis/stress

[0738] Gene-mediated effects can be assayed in cells showing mRNA expression. Luciferase reporter plasmids can be introduced by lipid-mediated transfection (TFX-50, Promega). Luciferase activity, an indicator of relative transcriptional activity, is measured by incubation of cell extracts with luciferin substrate and luminescence of the reaction is monitored in a luminometer.

[0739] Signaling pathways activated by 121P1F1 are mapped and used for the identification and validation of therapeutic targets. When 121P1F1 is involved in cell signaling, it is used as a target for diagnostic, prognostic, preventative and/or therapeutic purposes.

Example 46 Involvement in Tumor Progression

[0740] Based on the documented role of bZip and Steroid hormone receptor motifs in cell growth and proliferation (Holzel M et al, EMBO Rep. 2001, 2:1125), the 121P1F1 gene can contribute to the growth of cancer cells. The role of 121P1F1 in tumor growth is confirmed in a variety of primary and transfected cell lines including prostate, breast and kidney cell lines, as well as NIH 3T3 cells engineered to stably express 121P1F1. Parental cells lacking 121P1F1 and cells expressing 121P1F1 are evaluated for cell growth using a well-documented proliferation assay (Fraser S P, Grimes J A, Djamgoz M B. Prostate. 2000;44:61, Johnson D E, Ochieng J, Evans SL. Anticancer Drugs. 1996, 7:288).

[0741] To confirm the role of 121P1F1 in the transformation process, its effect in colony forming assays is investigated. Parental N1H-3T3 cells lacking 121P1F1 are compared to N1H-3T3 cells expressing 121P1F1, using a soft agar assay under stringent and more permissive conditions (Song Z. et al. Cancer Res. 2000;60:6730).

[0742] To confirm the role of 121P1F1 in invasion and metastasis of cancer cells, a well-established assay is used, e.g., a Transwell Insert System assay (Becton Dickinson) (Cancer Res. 1999; 59:6010). Control cells, including prostate, breast and kidney cell lines lacking 121P1F1 are compared to cells expressing 121P1F1. Cells are loaded with the fluorescent dye, calcein, and plated in the top well of the Transwell insert coated with a basement membrane analog. Invasion is determined by fluorescence of cells in the lower chamber relative to the fluorescence of the entire cell population.

[0743] 121P1F1 can also play a role in the regulation of the cell cycle and apoptosis. Parental cells and cells expressing 121P1F1 are compared for differences in cell cycle regulation using a well-established BrdU assay (Abdel-Malek Z A. J Cell Physiol. 1988, 136:247). In short, cells are grown under both optimal (full serum) and limiting (low serum) conditions, and are labeled with BrdU and stained with anti-BrdU Ab and propidium iodide. Cells are analyzed for entry into the G1, S, and G2M phases of the cell cycle. Alternatively, the effect of stress on apoptosis is evaluated in control parental cells and cells expressing 121P1F 1, including normal and tumor prostate, colon and lung cells. Engineered and parental cells are treated with various chemotherapeutic agents, such as etoposide, flutamide, etc, and protein synthesis inhibitors, such as cycloheximide. Cells are stained with annexin V-FITC and cell death is measured by FACS analysis. The modulation of cell death by 121P1F1 can play a critical role in regulating tumor progression and tumor load.

[0744] When 121P1F1 plays a role in cell growth, transformation, invasion or apoptosis, it is used as a target for diagnostic, prognostic, preventative and/or therapeutic purposes.

Example 47 Involvement in Angiogenesis

[0745] Angiogenesis or new capillary blood vessel formation is necessary for tumor growth (Hanahan D, Folkman J. Cell. 1996, 86:353; Folkman J. Endocrinology. 1998 139:441). Based on the effect of phsophodieseterase inhibitors on endothelial cells, 121P1F1 plays a role in angiogenesis (DeFouw L et al, Microvasc Res 2001, 62:263). Several assays have been developed to measure angiogenesis in vitro and in vivo, such as the tissue culture assays based on endothelial cell tube formation and endothelial cell proliferation. Using these assays as well as in vitro neo-vascularization, the role of 121P1F1 in angiogenesis, enhancement or inhibition, is confirmed.

[0746] For example, endothelial cells engineered to express 121P1F1 are evaluated using tube formation and proliferation assays. The effect of 121P1F1 is also confirmed in animal models in vivo. For example, cells either expressing or lacking 121P1F1 are implanted subcutaneously in immunocompromised mice. Endothelial cell migration and angiogenesis are evaluated 5-15 days later using immunohistochemistry techniques. Demonstration of an effect of 121P1F1 on angiogenesis confirms its usefulness as a target for diagnostic, prognostic, preventative and/or therapeutic purposes

Example 48 Involvement in Protein-Protein Interactions

[0747] Protein containing bZip motifs have been shown to interact with other proteins, specially proteins containing h.elix-loop-helix structures, thereby regulating gene transcription as well as cell growth (Schneider A et al, Curr Top Microbiol Immunol. 1997; 224:137; Amati B, Land H. Curr Opin Genet Dev. 1994, 4:102). Using immunoprecipitation techniques as well as two yeast hybrid systems, proteins are identified that associate with 121P1F1. Immunoprecipitates from cells expressing 121P1F1 and cells lacking 121P1F1 are compared for specific protein-protein associations.

[0748] Studies are performed to confirm the extent of association of 121P1F1 with effector molecules, such as nuclear proteins, transcription factors, kinases, phsophates etc. Studies comparing 121P1F1 positive and 121P1F1 negative cells as well as studies comparing unstimulated/resting cells and cells treated with epithelial cell activators, such as cytokines, growth factors, androgen and anti-integrin Ab reveal unique interactions.

[0749] In addition, protein-protein interactions are confirmed using two yeast hybrid methodology (Curr Opin Chem Biol. 1999, 3:64). A vector carrying a library of proteins fused to the activation domain of a transcription factor is introduced into yeast expressing a 121P1F1-DNA-binding domain fusion protein and a reporter construct. Protein-protein interaction is detected by colorimetric reporter activity. Specific association with effector molecules and transcription factors directs one of skill to the mode of action of 121P1F1, and thus identifies therapeutic, prognostic, preventative and/or diagnostic targets for cancer. This and similar assays are also used to identify and screen for small molecules that interact with 121P1F1.

[0750] Thus it is found that 121P1F1 associates with proteins and small molecules. Accordingly, 121P1F1 and these proteins and small molecules are used for diagnostic, prognostic, preventative and/or therapeutic purposes.

Example 49 Involvement in DNA-Protein Interactions

[0751] As previously mentioned, the basic-leucine zipper (bZIP) motif contain a basic region that mediates sequence-specific DNA-protein binding, as well as a leucine zipper region needed for protein dimerization. Electrophoretic mobility shift assays (EMSA) and DNA footprinting are used to identify 121P1F1-binding DNA sequences, and define specific response elements. In short, nuclear lysates are extracted from parental 121P1F1-negative as well as 121P1F1-expressing cells. The lysates are incubated in the presence of ³²P-labeled DNA probes. DNA-protein complexes are either separated by electrophoresis or exposed to a restriction nuclease, and analyzed by radiography. This process provides 121P1F1 specific DNA elements that are valuable tools in designing and testing inhibitors of 121P1F1.

[0752] When 121P1F1 functions as a transcription factor, it is used as a target for diagnostic, prognostic, preventative and therapeutic purposes.

[0753] Throughout this application, various website data content, publications, patent applications and patents are referenced. (Websites are referenced by their Uniform Resource Locator, or URL, addresses on the World Wide Web.) The disclosures of each of these references are hereby incorporated by reference herein in their entireties.

[0754] The present invention is not to be limited in scope by the embodiments disclosed herein, which are intended as single illustrations of individual aspects of the invention, and any that are functionally equivalent are within the scope of the invention. Various modifications to the models and methods of the invention, in addition to those described herein, will become apparent to those skilled in the art from the foregoing description and teachings, and are similarly intended to fall within the scope of the invention. Such modifications or other embodiments can be practiced without departing from the true scope and spirit of the invention.

[0755] Tables TABLE I Tissues that Express 121P1F1 When Malignant Prostate Bladder Kidney Colon Lung Pancreas Breast Cervix Stomach

[0756] TABLE II AMINO ACID ABBREVIATIONS SINGLE LETTER THREE LETTER FULL NAME F Phe phenylalanine L Leu leucine S Ser serine Y Tyr tyrosine C Cys cysteine W Trp tryptophan P Pro proline H His histidine Q Gln glutamine R Arg arginine I Ile isoleucine M Met methionine T Thr threonine N Asn asparagine K Lys lysine V Val valine A Ala alanine D Asp aspartic acid E Glu glutamic acid G Gly glycine

[0757] TABLE III AMINO ACID SUBSTITUTION MATRIX Adapted from the GCG Software 9.0 BLOSUM62 amino acid substitution matrix (block substitution matrix). The higher the value, the more likely a substitution is found in related, natural proteins. (See URL www.ikp.unibe.ch/manual/blosum62.html) A C D E F G H I K L M N P Q R S T V W Y . 4 0 −2 −1 −2 0 −2 −1 −1 −1 −1 −2 −1 −1 −1 1 0 0 −3 −2 A 9 −3 −4 −2 −3 −3 −1 −3 −1 −1 −3 −3 −3 −3 −1 −1 −1 −2 −2 C 6 2 −3 −1 −1 −3 −1 −4 −3 1 −1 0 −2 0 −1 −3 −4 −3 D 5 −3 −2 0 −3 1 −3 −2 0 −1 2 0 0 −1 −2 −3 −2 E 6 −3 −2 0 −3 0 0 −3 −4 −3 −3 −2 −2 −1 1 3 F 6 −2 −4 −2 −4 −3 0 −2 −2 −2 0 −2 −3 −2 −3 G 8 −3 −1 −3 −2 1 −2 0 0 −1 −2 −3 −2 2 H 4 −3 2 2 −3 −3 −3 −3 −2 −1 3 −3 −1 I 5 −2 −1 0 −1 2 2 0 −1 −2 −3 −2 K 4 2 −3 −3 −2 −2 −2 −1 1 −2 −1 L 5 −2 −2 0 −1 −1 −1 1 −1 −1 M 6 −2 0 0 1 0 −3 −4 −2 N 7 −1 −2 −1 −1 −2 −4 −3 P 5 1 0 −1 −2 −2 −1 Q 5 −1 −1 −3 −3 −2 R 4 1 −2 −3 −2 S 5 0 −2 −2 T 4 −3 −1 V 11 2 W 7 Y

[0758] TABLE IV (A) POSITION POSITION POSITION 2 (Primary Anchor) 3 (Primary Anchor) C Terminus (Primary Anchor) SUPERMOTIFS A1 TI LVMS FWY A2 LIVM ATQ IV MATL A3 VSMA TLI RK A24 YF WIVLMT FI YWLM B7 P VILF MWYA B27 RHK FYL WMIVA B44 E D FWYLIMVA B58 ATS FWY LIVMA B62 QL IVMP FWYMIVLA MOTIFS A1 TSM Y A1 DE AS Y A2.1 LM VQIAT V LIMAT A3 LMVISATF CGD KYR HFA A11 VTMLISAGN CDF K RYH A24 YF WM FLIW A*3101 MVT ALIS R K A*3301 MVALF IST RK A*6801 AVT MSLI RK B*0702 P LMF WYAIV B*3501 P LMFWY IVA B51 P LIVF WYAM B*5301 P IMFWY ALV B*5401 P ATIV LMFWY

[0759] TABLE IV (B) HLA CLASS II SUPERMOTIF 1 6 9 W, F, Y, V, .I, L A, V, I, L, P, C, S, T A, V, I, L, C, S, T, M, Y

[0760] TABLE IV (C) MOTIFS 1° anchor 1 2 3 4 5 1° anchor 6 7 8 9 DR4 preferred FMYLIVW M T I VSTCPALIM MH MH deleterious W R WDE DR1 preferred MFLIVWY PAMQ VMATSPLIC M AVM deleterious C CH FD CWD GDE D DR7 preferred MFLIVWY M W A IVMSACTPL M IV deleterious C G GRD N G DR3 MOTIFS 1° anchor 1 2 3 1° anchor 4 5 1° anchor 6 motif a LIVMFY D preferred motif b LIVMFAY DNQEST KRH preferred DR MFLIVWY VMSTACPLI Supermotif

[0761] TABLE IV (D) POSITION SUPER- MOTIFS POSITION: 1 2 3 4 5 6 7 8 C-terminus A1 $\frac{1{^\circ}\quad {Anchor}}{TILVMS}$

$\frac{1{^\circ}\quad {Anchor}}{FWY}$

A2 $\frac{1{^\circ}\quad {Anchor}}{LIVMATQ}$

$\frac{1{^\circ}\quad {Anchor}}{LIVMAT}$

A3 preferred $\frac{1{^\circ}\quad {Anchor}}{VSMATLI}$

YFW (4/5) YFW (3/5) YFW (4/5) P (4/5) $\frac{1{^\circ}\quad {Anchor}}{RK}$

deleterious DE (3/5); DE P (5/5) (4/5) A24 $\frac{1{^\circ}\quad {Anchor}}{YFWIVLMT}$

$\frac{1{^\circ}\quad {Anchor}}{FIYWLM}$

B7 preferred FWY (5/5) LIVM (3/5) $\frac{1{^\circ}\quad {Anchor}}{P}$

FWY (4/5) FWY (3/5) $\frac{1{^\circ}\quad {Anchor}}{VILFMWYA}$

deleterious DE (3/5); DE G QN DE P(5/5); (3/5) (4/5) (4/5) (4/5) G(4/5); A(3/5); QN(3/5) B27 $\frac{1{^\circ}\quad {Anchor}}{RHK}$

$\frac{1{^\circ}\quad {Anchor}}{FYLWMIVA}$

B44 $\frac{1{^\circ}\quad {Anchor}}{ED}$

$\frac{1{^\circ}\quad {Anchor}}{FWYLIMVA}$

B58 $\frac{1{^\circ}\quad {Anchor}}{ATS}$

$\frac{1{^\circ}\quad {Anchor}}{FWYLIVMA}$

B62 $\frac{1{^\circ}\quad {Anchor}}{QLIVMP}$

$\frac{1{^\circ}\quad {Anchor}}{FWYMIVLA}$

[0762] TABLE IV (E) 9 or POSITION: 1 2 3 4 5 6 7 8 C-terminus C-terminus A1 9-mer preferred GFYW $\frac{1{^\circ}\quad {Anchor}}{STM}$

DEA YFW P DEQN YFW $\frac{1{^\circ}\quad {Anchor}}{Y}$

deleterious DE RHKLIVMP A G A A1 9-mer preferred GRHK ASTCLIVM $\frac{1{^\circ}\quad {Anchor}}{DEAS}$

GSTC ASTC LIVM DE $\frac{1{^\circ}\quad {Anchor}}{Y}$

deleterious A RHKDEPY DE PQN RHK PG GP FW A1 10-mer preferred YFW $\frac{1{^\circ}\quad {Anchor}}{STM}$

DEAQN A YFWQN PASTC GDE P $\frac{1{^\circ}\quad {Anchor}}{Y}$

deleterious GP RHKGLIVM DE RHK QNA RHKYFW RHK A A1 10-mer preferred YFW STCLIVM $\frac{1{^\circ}\quad {Anchor}}{DEAS}$

A YFW PG G YFW $\frac{1{^\circ}\quad {Anchor}}{Y}$

deletetious RHK RHKDEPY P G PRHK QN FW A2.1 9-mer preferred YFW $\frac{1{^\circ}\quad {Anchor}}{LMIVQAT}$

YFW STC YFW A P $\frac{1{^\circ}\quad {Anchor}}{VLIMAT}$

deleterious DEP DERKH RKH DERKH A2.1 10-mer preferred AYFW $\frac{1{^\circ}\quad {Anchor}}{LMIVQAT}$

LVIM G G FYWL VIM $\frac{1{^\circ}\quad {Anchor}}{VLIMAT}$

deleterious DEP DE RKHA P RKH DERKLH RKH A3 preferred RHK $\frac{1{^\circ}\quad {Anchor}}{LMVISATGCGD}$

YFW PRHKK YFW A YFW P $\frac{1{^\circ}\quad {Anchor}}{KYRHFA}$

deleterious DEP DE A11 preferred A $\frac{1{^\circ}\quad {Anchor}}{VTLMISAGNCDF}$

YFW YFW A YFW YFW P $\frac{1{^\circ}\quad {Anchor}}{KRYH}$

deleterious DEP A G A24 9-mer preferred YFWRHK $\frac{1{^\circ}\quad {Anchor}}{YFWM}$

STC YFW YFW $\frac{1{^\circ}\quad {Anchor}}{FLIW}$

deleterious DEG DE G QNP DERH G AQN K A24 10-mer preferred $\frac{1{^\circ}\quad {Anchor}}{YFWM}$

P YFWP P $\frac{1{^\circ}\quad {Anchor}}{FLIW}$

deleterious GDE QN RHK DE A QN DEA A3101 preferred RHK $\frac{1{^\circ}\quad {Anchor}}{MVTALIS}$

YFW P YFW YFW AP $\frac{1{^\circ}\quad {Anchor}}{RK}$

deleterious DEP DE ADE DE DE DE A3301 preferred $\frac{1{^\circ}\quad {Anchor}}{MVALFIST}$

YFW AYFW $\frac{1{^\circ}\quad {Anchor}}{RK}$

deleterious GP DE A6801 preferred YFWSTC $\frac{1{^\circ}\quad {Anchor}}{AVTMSLI}$

YFWLIVM YFW P $\frac{1{^\circ}\quad {Anchor}}{RK}$

deleterious GP DEG RHK A B0702 preferred RHKFWY $\frac{1{^\circ}\quad {Anchor}}{P}$

RHK RHK RHK RHK PA $\frac{1{^\circ}\quad {Anchor}}{LMFWYAIV}$

deleterious DEQNP DEP DE DE GDE QN DE B3501 preferred FWYLIVM $\frac{1{^\circ}\quad {Anchor}}{P}$

FWY $\frac{1{^\circ}\quad {Anchor}}{LMFWYIVA}$

deleterious AGP G G B51 preferred LIVMFWY $\frac{1{^\circ}\quad {Anchor}}{P}$

FWY STC FWY G FWY $\frac{1{^\circ}\quad {Anchor}}{LIVFWYAM}$

deleterious AGPDER HKSTC DE G DEQN GDE B5301 preferred LIVMFWY $\frac{1{^\circ}\quad {Anchor}}{P}$

FWY STC FWY LIVMFWY FWY $\frac{1{^\circ}\quad {Anchor}}{IMFWYALV}$

deleterious AGPQN G RHKQN DE B5401 preferred FWY $\frac{1{^\circ}\quad {Anchor}}{P}$

FWYL IVM LIVM ALIVM FWYAP $\frac{1{^\circ}\quad {Anchor}}{ATIVLMFWY}$

deleterious GPQNDE GDES TC RHKDE DE QNDGE DE

[0763] TABLE V (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A1, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 169 WTDNIFAIK 50.000 1. 2 114 RCETEERTR 9.000 2. 3 16 MMEIFSETK 9.000 3. 4 195 FGIPEDFDY 6.250 4. 5 106 SIEKAKIGR 4.500 5. 6 20 FSETKDVFQ 2.700 6. 7 59 MVDCERIGT 2.500 7. 8 185 GFEENKIDR 2.250 8. 9 116 ETEERTRLA 2.250 9. 10 152 VEEIRQANK 1.800 10. 11 101 ASLQKSIEK 1.500 11. 12 93 LSEGSQKHA 1.350 12. 13 54 LVDDGMVDC 1.000 13. 14 146 DCDPQVVEE 1.000 14. 15 85 KLEVLESQL 0.900 15. 16 151 VVEEIRQAN 0.900 16. 17 8 SAEEKRTRM 0.900 17. 18 88 VLESQLSEG 0.900 18. 19 130 LRDQREQLK 0.500 19. 20 117 TEERTRLAK 0.450 20. 21 193 RTFGIPEDF 0.250 21. 22 66 GTSNYYWAF 0.250 22. 23 77 KALHARKHK 0.200 23. 24 72 WAFPSKALH 0.200 24. 25 138 KAEVEKYKD 0.180 25. 26 7 LSAEEKRTR 0.150 26. 27 126 ELSSLRDQR 0.100 27. 28 34 KIAPKEKGI 0.100 28. 29 61 DCERIGTSN 0.090 29. 30 133 QREQLKAEV 0.090 30. 31 40 KGITAMSVK 0.050 31. 32 22 ETKDVFQLK 0.050 32. 33 26 VFQLKDLEK 0.050 33. 34 136 QLKAEVEKY 0.050 34. 35 197 IPEDFDYID 0.045 35. 36 47 VKEVLQSLV 0.045 36. 37 162 AKEAANRWT 0.045 37. 38 186 FEENKIDRT 0.045 38. 39 91 SQLSEGSQK 0.030 39. 40 63 ERIGTSNYY 0.025 40. 41 42 ITAMSVKEV 0.025 41. 42 5 KGLSAEEKR 0.025 42. 43 144 YKDCDPQVV 0.025 43. 44 148 DPQVVEEIR 0.025 44. 45 124 AKELSSLRD 0.022 45. 46 175 AIKSWAKRK 0.020 46. 47 174 FAIKSWAKR 0.020 47. 48 30 KDLEKIAPK 0.020 48. 49 155 IRQANKVAK 0.020 49. 50 160 KVAKEAANR 0.020 50.

[0764] TABLE VI (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A1, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 116 ETEERTRLAK 225.000 51. 2 151 VVEEIRQANK 36.000 52. 3 20 FSETKDVFQL 6.750 53. 4 169 WTDNIFAIKS 6.250 54. 5 146 DCDPQVVEEI 5.000 55. 6 61 DCERIGTSNY 4.500 56. 7 31 DLEKIAPKEK 1.800 57. 8 93 LSEGSQKHAS 1.350 58. 9 25 DVFQLKDLEK 1.000 59. 10 100 HASLQKSIEK 1.000 60. 11 29 LKDLEKIAPK 1.000 61. 12 8 SAEEKRTRMM 0.900 62. 13 85 KLEVLESQLS 0.900 63. 14 88 VLESQLSEGS 0.900 64. 15 138 KAEVEKYKDC 0.900 65. 16 114 RCETEERTRL 0.900 66. 17 105 KSIEKAKIGR 0.750 67. 18 72 WAFPSKALHA 0.500 68. 19 59 MVDCERIGTS 0.500 69. 20 186 FEENKIDRTF 0.450 70. 21 90 ESQLSEGSQK 0.300 71. 22 55 VDDGMVDCER 0.250 72. 23 172 NIFAIKSWAK 0.200 73. 24 96 GSQKHASLQK 0.150 74. 25 184 FGFEENKIDR 0.125 75. 26 194 TFGIPEDFDY 0.125 76. 27 130 LRDQREQLKA 0.125 77. 28 18 EIFSETKDVF 0.100 78. 29 6 GLSAEEKRTR 0.100 79. 30 34 KIAPKEKGIT 0.100 80. 31 15 RMMEIFSETK 0.100 81. 32 68 SNYYWAFPSK 0.100 82. 33 106 SIEKAKIGRC 0.090 83. 34 177 KSWAKRKFGF 0.075 84. 35 67 TSNYYWAFPS 0.075 85. 36 54 LVDDGMVDCE 0.050 86. 37 185 GFEENKIDRT 0.045 87. 38 124 AKELSSLRDQ 0.045 88. 39 152 VEEIRQANKV 0.045 89. 40 16 MMEIFSETKD 0.045 90. 41 154 EIRQANKVAK 0.040 91. 42 65 IGTSNYYWAF 0.025 92. 43 42 ITAMSVKEVL 0.025 93. 44 23 TKDVFQLKDL 0.025 94. 45 190 KIDRTFGIPE 0.025 95. 46 58 GMVDCERIGT 0.025 96. 47 195 FGIPEDFDYI 0.025 97. 48 44 AMSVKEVLQS 0.025 98. 49 47 VKEVLQSLVD 0.022 99. 50 174 FAIKSWAKRK 0.020 100

[0765] TABLE VII (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A2, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 15 RMMEIFSET 155.125 101. 2 122 RLAKELSSL 49.134 102. 3 196 GIPEDFDYI 30.116 103. 4 78 ALHARKHKL 21.362 104. 5 27 FQLKDLEKI 20.290 105. 6 172 NIFAIKSWA 13.901 106. 7 6 GLSAEEKRT 7.452 107. 8 102 SLQKSIEKA 5.599 108. 9 21 SETKDVFQL 5.541 109. 10 34 KIAPKEKGI 5.021 110. 11 85 KLEVLESQL 4.785 111. 12 42 ITAMSVKEV 3.777 112. 13 129 SLRDQREQL 3.262 113. 14 54 LVDDGMVDC 2.787 114. 15 18 EIFSETKDV 2.654 115. 16 115 CETEERTRL 1.703 116. 17 150 QVVEEIRQA 0.820 117. 18 46 SVKEVLQSL 0.617 118. 19 139 AEVEKYKDC 0.594 119. 20 65 IGTSNYYWA 0.455 120. 21 59 MVDCERIGT 0.443 121. 22 51 LQSLVDDGM 0.420 122. 23 189 NKIDRTFGI 0.345 123. 24 92 QLSEGSQKH 0.306 124. 25 28 QLKDLEKIA 0.292 125. 26 24 KDVFQLKDL 0.239 126. 27 43 TAMSVKEVL 0.221 127. 28 52 QSLVDDGMV 0.218 128. 29 50 VLQSLVDDG 0.143 129. 30 153 EELRQANKV 0.101 130. 31 70 YYWAFPSKA 0.100 131. 32 168 RWTDNIFAI 0.079 132. 33 177 KSWAKRKFG 0.078 133. 34 144 YKDCDPQVV 0.073 134. 35 165 AANRWTDNI 0.071 135. 36 157 QANKVAKEA 0.069 136. 37 64 RIGTSNYYW 0.056 137. 38 186 FEENKIDRT 0.048 138. 39 167 NRWTDNIFA 0.031 139. 40 183 KFGFEENKI 0.025 140. 41 99 KHASLQKSI 0.025 141. 42 53 SLVDDGMVD 0.025 142. 43 88 VLESQLSEG 0.019 143. 44 8 SAEEKRTRM 0.018 144. 45 5 8GMVDCERIG 0.018 145. 46 72 WAFPSKALH 0.018 146. 47 147 CDPQVVEEI 0.016 147. 48 104 QKSIEKAKI 0.014 148. 49 71 YWAFPSKAL 0.014 149. 50 195 FGIPEDFDY 0.013 150.

[0766] TABLE VIII (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A2, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 53 SLVDDGMVDC 46.848 151. 2 58 GMVDCERIGT 22.066 152. 3 41 GITAMSVKEV 21.996 153. 4 92 QLSEGSQKHA 20.369 154. 5 64 RIGTSNYYWA 5.636 155. 6 50 VLQSLVDDGM 4.138 156. 7 77 KALHARKHKL 3.842 157. 8 27 FQLKDLEKIA 3.515 158. 9 17 MEIFSETKDV 2.299 159. 10 195 FGIPEDFDYI 1.604 160. 11 51 LQSLVDDGMV 1.558 161. 12 72 WAFPSKALHA 1.174 162. 13 46 SVKEVLQSLV 0.873 163. 14 5 KGLSAEEKRT 0.630 164. 15 20 FSETKDVFQL 0.548 165. 16 45 MSVKEVLQSL 0.545 166. 17 156 RQANKVAKEA 0.504 167. 18 94 SEGSQKHASL 0.415 168. 19 15 RMMEIFSETK 0.304 169. 20 128 SSLRDQREQL 0.253 170. 21 7 LSAEEKRTRM 0.226 171. 22 34 KIAPKEKGIT 0.191 172. 23 38 KEKGITAMSV 0.166 173. 24 132 DQREQLKAEV 0.165 174. 25 167 NRWTDNIFAI 0.160 175. 26 152 VEEIRQANKV 0.147 176. 27 101 ASLQKSIEKA 0.135 177. 28 44 AMSVKEVLQS 0.124 178. 29 35 IAPKEKGITA 0.117 179. 30 70 YYWAFPSKAL 0.113 180. 31 42 ITAMSVKEVL 0.101 181. 32 79 LHARKHKLEV 0.082 182. 33 177 KSWAKRKFGF 0.082 183. 34 115 CETEERTRLA 0.079 184. 35 103 LQKSIEKAKI 0.063 185. 36 172 NIFAIKSWAK 0.057 186. 37 182 RKFGFEENKI 0.054 187. 38 157 QANKVAKEAA 0.034 188. 39 91 SQLSEGSQKH 0.028 189. 40 161 VAKEAANRWT 0.028 190. 41 23 TKDVFQLKDL 0.027 191. 42 150 QVVEEIRQAN 0.027 192. 43 121 TRLAKELSSL 0.025 193. 44 142 EKYKDCDPQV 0.023 194. 45 138 KAEVEKYKDC 0.023 195. 46 160 KVAKEAANRW 0.023 196. 47 87 EVLESQLSEG 0.017 197. 48 85 KLEVLESQLS 0.017 198. 49 84 HKLEVLESQL 0.015 199. 50 102 SLQKSIEKAK 0.015 200.

[0767] TABLE IX (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A3, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 16 MMEIFSETK 60.000 201. 2 136 QLKAEVEKY 12.000 202. 3 169 WTDNIFAIK 4.500 203. 4 175 AIKSWAKRK 3.000 204. 5 66 GTSNYYWAF 2.700 205. 6 85 KLEVLESQL 1.800 206. 7 22 ETKDVFQLK 1.350 207. 8 97 SQKHASLQK 1.200 208. 9 160 KVAKEAANR 1.200 209. 10 12 6ELSSLRDQR 1.200 210. 11 193 RTFGIPEDF 1.125 211. 12 15 RMMEIFSET 1.012 212. 13 122 RLAKELSSL 0.900 213. 14 91 SQLSEGSQK 0.900 214. 15 196 GIPEDFDYI 0.810 215. 16 106 SIEKAKIGR 0.800 216. 17 78 ALHARKHKL 0.600 217. 18 129 SLRDQREQL 0.600 218. 19 77 KALHARKHK 0.450 219. 20 103 LQKSIEKAK 0.450 220. 21 182 RKFGFEENK 0.450 221. 22 102 SLQKSIEKA 0.300 222. 23 92 QLSEGSQKH 0.300 223. 24 101 ASLQKSIEK 0.300 224. 25 69 NYYWAFPSK 0.300 225. 26 135 EQLKAEVEK 0.270 226. 27 30 KDLEKIAPK 0.203 227. 28 46 SVKEVLQSL 0.203 228. 29 172 NIFAIKSWA 0.150 229. 30 6 GLSAEEKRT 0.150 230. 31 40 KGITAMSVK 0.135 231. 32 34 KIAPKEKGI 0.135 232. 33 117 TEERTRLAK 0.120 233. 34 28 QLKDLEKIA 0.100 234. 35 4 KKGLSAEEK 0.060 235. 36 173 IFAIKSWAK 0.060 236. 37 50 VLQSLVDDG 0.060 237. 38 174 FAIKSWAKR 0.060 238. 39 152 VEEIRQANK 0.060 239. 40 64 RIGTSNYYW 0.060 240. 41 123 LAKELSSLR 0.060 241. 42 74 FPSKALHAR 0.060 242. 43 53 SLVDDGMVD 0.060 243. 44 27 FQLKDLEKI 0.041 244. 45 26 VFQLKDLEK 0.040 245. 46 185 GFEENKIDR 0.036 246. 47 54 LVDDGMVDC 0.030 247. 48 32 LEKIAPKEK 0.030 248. 49 88 VLESQLSEG 0.030 249. 50 195 FGIPEDFDY 0.027 250.

[0768] TABLE X (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A3, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 15 RMMEIFSETK 135.000 251. 2 172 NIFAIKSWAK 30.000 252. 3 129 SLRDQREQLK 20.000 253. 4 136 QLKAEVEKYK 15.000 254. 5 102 SLQKSIEKAK 15.000 255. 6 25 DVFQLKDLEK 6.000 256. 7 122 RLAKELSSLR 4.000 257. 8 31 DLEKIAPKEK 3.000 258. 9 151 VVEEIRQANK 3.000 259. 10 6 GLSAEEKRTR 1.200 260. 11 111 KIGRCETEER 1.200 261. 12 58 GMVDCERIGT 0.900 262. 13 116 ETEERTRLAK 0.900 263. 14 154 EIRQANKVAK 0.600 264. 15 96 GSQKHASLQK 0.600 265. 16 68 SNYYWAFPSK 0.600 266. 17 53 SLVDDGMVDC 0.450 267. 18 174 FAIKSWAKRK 0.450 268. 19 177 KSWAKRKFGF 0.450 269. 20 100 HASLQKSIEK 0.400 270. 21 50 VLQSLVDDGM 0.300 271. 22 18 EIFSETKDVF 0.300 272. 23 105 KSIEKAKIGR 0.270 273. 24 21 SETKDVFQLK 0.270 274. 25 44 AMSVKEVLQS 0.240 275. 26 74 FPSKALHARK 0.200 276. 27 181 KRKFGFEENK 0.180 277. 28 135 EQLKAEVEKY 0.162 278. 29 92 QLSEGSQKHA 0.150 279. 30 85 KLEVLESQLS 0.120 280. 31 3 KKKGLSAEEK 0.090 281. 32 168 RWTDNIFAIK 0.090 282. 33 41 GITAMSVKEV 0.090 283. 34 196 GIPEDFDYID 0.081 284. 35 184 FGFEENKIDR 0.060 285. 36 134 REQLKAEVEK 0.060 286. 37 64 RIGTSNYYWA 0.060 287. 38 160 KVAKEAANRW 0.060 288. 39 125 KELSSLRDQR 0.054 289. 40 42 ITAMSVKEVL 0.045 290. 41 28 QLKDLEKIAP 0.040 291. 42 88 VLESQLSEGS 0.040 292. 43 190 KIDRTFGIPE 0.036 293. 44 29 LKDLEKIAPK 0.030 294. 45 46 SVKEVLQSLV 0.030 295. 46 72 WAFPSKALHA 0.030 296. 47 90 ESQLSEGSQK 0.030 297. 48 77 KALHARKHKL 0.027 298. 49 20 FSETKIDVFQL 0.027 299. 50 165 AANRWTDNIF 0.020 300.

[0769] TABLE XI (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A11, 9-MERS +HC,9 SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 160 KVAKEAANR 1.200 301. 2 97 SQKHASLQK 1.200 302. 3 169 WTDNIFAIK 1.000 303. 4 91 SQLSEGSQK 0.900 304. 5 69 NYYWAFPSK 0.800 305. 6 77 KALHARKHK 0.450 306. 7 16 MMEIFSETK 0.400 307. 8 173 IFAIKSWAK 0.400 308. 9 26 VFQLKDLEK 0.400 309. 10 103 LQKSIEKAK 0.300 310. 11 22 ETKDVFQLK 0.300 311. 12 135 EQLKAEVEK 0.270 312. 13 185 GFEENKIDR 0.240 313. 14 175 AIKSWAKRK 0.200 314. 15 106 SIEKAKIGR 0.160 315. 16 182 RKFGFEENK 0.120 316. 17 117 TEERTRLAK 0.120 317. 18 40 KGITAMSVK 0.090 318. 19 30 KDLEKIAPK 0.090 319. 20 101 ASLQKSIEK 0.060 320. 21 4 KKGLSAEEK 0.060 321. 22 152 VEEIRQANK 0.060 322. 23 174 FAIKSWAKR 0.060 323. 24 66 GTSNYYWAF 0.060 324. 25 193 RTFGIPEDF 0.060 325. 26 123 LAKELSSLR 0.040 326. 27 74 FPSKALHAR 0.040 327. 28 32 LEKIAPKEK 0.030 328. 29 126 ELSSLRDQR 0.024 329. 30 64 RIGTSNYYW 0.024 330. 31 46 SVKEVLQSL 0.020 331. 32 155 IRQANKVAK 0.020 332. 33 130 LRDQREQLK 0.020 333. 34 5 KGLSAEEKR 0.018 334. 35 114 RCETEERTR 0.012 336. 36 148 DPQVVEEIR 0.012 336. 37 196 GIPEDFDYI 0.012 337. 38 85 KLEVLESQL 0.012 338. 39 122 RLAKELSSL 0.012 339. 40 143 KYKDCDPQV 0.012 340. 41 137 LKAEVEKYK 0.010 341. 42 27 FQLKDLEKI 0.009 342. 43 172 NIFAIKSWA 0.008 343. 44 70 YYWAFPSKA 0.008 344. 45 34 KIAPKEKGI 0.006 345. 46 51 LQSLVDDGM 0.006 346. 47 13 RTRMMEIFS 0.006 347. 48 183 KFGFEENKI 0.006 348. 49 42 ITAMSVKEV 0.005 349. 50 136 QLKAEVEKY 0.004 350.

[0770] TABLE XII (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A11, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 15 RMMEIFSETK 2.400 351. 2 25 DVFQLKDLEK 2.400 352. 3 151 VVEEIRQANK 2.000 353. 4 172 NIFAIKSWAK 1.600 354. 5 116 ETEERTRLAK 0.600 355. 6 100 HASLQKSIEK 0.400 356. 7 129 SLRDQREQLK 0.400 357. 8 111 KIGRCETEER 0.240 358. 9 122 RLAKELSSLR 0.240 359. 10 136 QLKAEVEKYK 0.200 360. 11 102 SLQKSIEKAK 0.200 361. 12 74 FPSKALHARK 0.200 362. 13 134 REQLKAEVEK 0.180 363. 14 174 FAIKSWAKRK 0.150 364. 15 96 GSQKHASLQK 0.120 365. 16 154 EIRQANKVAK 0.120 366. 17 68 SNYYWAFPSK 0.080 367. 18 181 KRKFGFEENK 0.060 368. 19 3 KKKGLSAEEK 0.060 369. 20 168 RWTDNIFAIK 0.060 370. 21 21 SETKDVFQLK 0.060 371. 22 31 DLEKIAPKEK 0.060 372. 23 160 KVAKEAANRW 0.060 373. 24 125 KELSSLRDQR 0.054 374. 25 73 AFPSKALHAR 0.040 375. 26 173 IFAIKSWAKR 0.040 376. 27 105 KSIEKAKIGR 0.036 377. 28 6 GLSAEEKRTR 0.024 378. 29 64 RIGTSNYYWA 0.024 379. 30 29 LKDLEKIAPK 0.020 380. 31 46 SVKEVLQSLV 0.020 381. 32 18 4FGFEENKIDR 0.016 382. 33 4 KKGLSAEEKR 0.012 383. 34 143 KYKDCDPQVV 0.012 384. 35 42 ITAMSVKEVL 0.010 385. 36 76 SKALHARKHK 0.010 386. 37 156 RQANKVAKEA 0.009 387. 38 77 KALHARKHKL 0.009 388. 39 13 RTRMMEIFSE 0.009 389. 40 91 SQLSEGSQKH 0.009 390. 41 69 NYYWAFPSKA 0.008 391. 42 72 WAFPSKALHA 0.008 392. 43 159 NKVAKEAANR 0.006 393. 44 39 EKGITAMSVK 0.006 394. 45 114 RCETEERTRL 0.006 395. 46 120 RTRLAKELSS 0.006 396. 47 51 LQSLVDDGMV 0.006 397. 48 90 ESQLSEGSQK 0.006 398. 49 103 LQKSIEKAKI 0.006 399. 50 193 RTFGIPEDFD 0.006 400.

[0771] TABLE XIII (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A24, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 85 KLEVLESQL 14.400 401. 2 183 KFGFEENKI 13.200 402. 3 143 KYKDCDPQV 12.000 403. 4 19 IFSETKDVF 12.000 404. 5 43 TAMSVKEVL 8.400 405. 6 46 SVKEVLQSL 8.064 406. 7 122 RLAKELSSL 8.000 407. 8 193 RTFGIPEDF 5.600 408. 9 70 YYWAFPSKA 5.500 409. 10 129 SLRDQREQL 4.800 410. 11 78 ALHARKHKL 4.400 411. 12 71 YWAFPSKAL 4.000 412. 13 95 EGSQKHASL 4.000 413. 14 166 ANRWTDNIF 2.400 414. 15 34 KIAPKEKGI 2.400 415. 16 168 RWTDNIFAI 2.400 416. 17 196 GIPEDFDYI 2.160 417. 18 178 SWAKRKFGF 2.000 418. 19 66 GTSNYYWAF 2.000 419. 20 27 FQLKDLEKI 1.650 420. 21 165 AANRWTDNI 1.500 421. 22 57 DGMVDCERI 1.500 422. 23 24 KDVFQLKDL 1.200 423. 24 8 SAEEKRTRM 0.900 424. 25 73 AFPSKALHA 0.750 425. 26 51 LQSLVDDGM 0.700 426. 27 15 RMMEIFSET 0.665 427. 28 69 NYYWAFPSK 0.600 428. 29 11 9ERTRLAKEL 0.528 429. 30 11 5CETEERTRL 0.480 430. 31 187 EENKIDRTF 0.420 431. 32 12 KRTRMMEIF 0.400 432. 33 81 ARKHKLEVL 0.400 433. 34 21 SETKDVFQL 0.400 434. 35 151 VVEEIRQAN 0.302 435. 36 99 KHASLQKSI 0.240 436. 37 147 CDPQVVEEI 0.231 437. 38 157 QANKVAKEA 0.231 438. 39 176 IKSWAKRKF 0.220 439. 40 109 KAKIGRCET 0.220 440. 41 61 DCERIGTSN 0.210 441. 42 13 RTRMMEIFS 0.200 442. 43 120 RTRLAKELS 0.200 443. 44 64 RIGTSNYYW 0.200 444. 45 189 NKIDRTFGI 0.180 445. 46 150 QVVEEIRQA 0.180 446. 47 195 FGIPEDFDY 0.180 447. 48 116 ETEERTRLA 0.180 448. 49 102 SLQKSIEKA 0.165 449. 50 171 DNIFAIKSW 0.150 450.

[0772] TABLE XIV (A) HLA PEPTIDE SCORING RESULTS-121P1F1-A24, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 70 YYWAFPSKAL 200.000 451. 2 143 KYKDCDPQVV 14.400 452. 3 77 KALHARKHKL 13.200 453. 4 114 RCETEERTRL 12.000 454. 5 45 MSVKEVLQSL 10.080 454. 6 26 VFQLKDLEKI 8.250 456. 7 20 FSETKDVFQL 6.000 457. 8 128 SSLRDQREQL 6.000 458. 9 42 ITAMSVKEVL 5.600 459. 10 69 NYYWAFPSKA 5.500 460. 11 80 HARKHKLEVL 4.000 461. 12 177 KSWAKRKFGF 4.000 462. 13 165 AANRWTDNIF 3.600 463. 14 175 AIKSWAKRKF 2.200 464. 15 195 FGIPEDFDYI 2.160 465. 16 18 EIFSETKDVF 2.000 466. 17 65 IGTSNYYWAF 2.000 467. 18 146 DCDPQVVEEI 1.848 468. 19 103 LQKSIEKAKI 1.100 469. 20 50 VLQSLVDDGM 1.050 470. 21 188 ENKIDRTFGI 1.000 471. 22 164 EAANRWTDNI 1.000 472. 23 8 SAEEKRTRMM 0.900 473. 24 185 GFEENKIDRT 0.900 474. 25 84 HKLEVLESQL 0.864 475. 26 121 TRLAKELSSL 0.600 476. 27 36 APKEKGITAM 0.600 477. 28 7 LSAEEKRTRM 0.600 478. 29 118 EERTRLAKEL 0.528 479. 30 194 TFGIPEDFDY 0.500 480. 31 186 FEENKIDRTF 0.420 481. 32 23 TKDVFQLKDL 0.400 482. 33 94 SEGSQKHASL 0.400 483. 34 85 KLEVLESQLS 0.360 484. 35 156 RQANKVAKEA 0.308 485. 36 150 QVVEEIRQAN 0.302 486. 37 138 KAEVEKYKDC 0.300 487. 38 5 KGLSAEEKRT 0.300 488. 39 192 DRTFGIPEDF 0.280 489. 40 182 RKFGFEENKI 0.264 490. 41 34 KIAPKEKGIT 0.240 491. 42 160 KVAKEAANRW 0.240 492. 43 171 DNIFAIKSWA 0.210 493. 44 64 RIGTSNYYWA 0.200 494. 45 11 EKRTRMMEIF 0.200 495. 46 120 RTRLAKELSS 0.200 496. 47 27 FQLKDLEKJA 0.180 497. 48 88 VLESQLSEGS 0.180 498. 49 58 GMVDCERIGT 0.180 499. 50 53 SLVDDGMVDC 0.180 500.

[0773] TABLE XV (A) HLA PEPTIDE SCORING RESULTS-121P1F1-B7, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 129 SLRDQREQL 60.000 501. 2 43 TAMSVKEVL 36.000 502. 3 46 SVKEVLQSL 20.000 503. 4 78 ALHARKHKL 12.000 504. 5 36 APKEKGITA 6.000 505. 6 80 HARKHKLEV 6.000 506. 7 122 RLAKELSSL 4.000 507. 8 95 EGSQKHASL 4.000 508. 9 165 AANRWTDNI 3.600 509. 10 8 SAEEKRTRM 1.350 510. 11 85 KLEVLESQL 1.200 511. 12 81 ARKHKLEVL 1.200 512. 13 57 DGMVDCERI 1.200 513. 14 51 LQSLVDDGM 1.000 514. 15 154 EIRQANKVA 1.000 515. 16 115 CETEERTRI 0.600 516. 17 71 YWAFPSKAL 0.600 517. 18 166 ANRWTDNIF 0.600 518. 19 150 QVVEEIRQA 0.500 519. 20 109 KAKIGRCET 0.450 520. 21 27 FQLKDLEKI 0.400 521. 22 11 EKRTRMMEI 0.400 522. 23 21 SETKDVFQL 0.400 523. 24 196 GIPEDFDYI 0.400 524. 25 34 KIAPKEKGI 0.400 525. 26 119 ERTRLAKEL 0.400 526. 27 24 KDVFQLKDL 0.400 527. 28 35 IAPKEKGIT 0.300 528. 29 15 RMMEIFSET 0.300 529. 30 158 ANKVAKEAA 0.300 530. 31 157 QANKVAKEA 0.300 531. 32 59 MVDCERIGT 0.225 532. 33 148 DPQVVEEIR 0.200 533. 34 18 EIFSETKDV 0.200 534. 35 52 QSLVDDGMV 0.200 535. 36 74 FPSKALHAR 0.200 536. 37 120 RTRLAKELS 0.200 537. 38 13 RTRMMEIFS 0.200 538. 39 42 ITAMSVKEV 0.200 539. 40 54 LVDDGMVDC 0.150 540. 41 65 IGTSNYYWA 0.100 541. 42 102 SLQKSIEKA 0.100 542. 43 132 DQREQLKAE 0.100 543. 44 1 MSKKKGLSA 0.100 544. 45 112 IGRCETEER 0.100 545. 46 6 GLSAEEKRT 0.100 546. 47 28 QLKDLEKIA 0.100 547. 48 172 NIFAIKSWA 0.100 548. 49 9 AEEKRTRMM 0.090 549. 50 164 EAANRWTDN 0.060 550.

[0774] TABLE XVI (A) HLA PEPTIDE SCORING RESULTS-121P1F1-B7, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 80 HARKHKLEVL 120.000 551. 2 36 APKEKGITAM 60.000 552. 3 77 KALHARKHKL 12.000 553. 4 128 SSLRDQREQL 6.000 554. 5 42 ITAMSVKEVL 4.000 555. 6 45 MSVKEVLQSL 4.000 556. 7 118 EERTRLAKEL 4.000 557. 8 166 ANRWTDNIFA 3.000 558. 9 132 DQREQLKAEV 2.000 559. 10 114 RCETEERTRL 1.800 560. 11 7 LSAEEKRTRM 1.500 561. 12 20 FSETKDVFQL 1.200 562. 13 164 EAANRWTDNI 1.200 563. 14 46 SVKEVLQSLV 1.000 564. 15 50 VLQSLVDDGM 1.000 565. 16 112 IGRCETEERT 1.000 566. 17 8 SAEEKRTRMM 0.900 567. 18 70 YYWAFPSKAL 0.600 568. 19 94 SEGSQKHASL 0.400 569. 20 188 ENKIDRTFGI 0.400 570. 21 103 LQKSIEKAKI 0.400 571. 22 121 TRLAKELSSL 0.400 572. 23 195 FGIPEDFDYI 0.400 573. 24 84 HKLEVLESQL 0.400 574. 25 72 WAFPSKALHA 0.300 575. 26 35 IAPKEKGITA 0.300 576. 27 101 ASLQKSIEKA 0.300 577. 28 157 QANKVAKEAA 0.300 578. 29 161 VAKEAANRWT 0.300 579. 30 120 RTRLAKELSS 0.200 580. 31 41 GITAMSVKEV 0.200 581. 32 148 DPQVVEEIRQ 0.200 582. 33 51 LQSLVDDGMV 0.200 583. 34 74 FPSKALHARK 0.200 584. 35 165 AANRWTDNIF 0.180 585. 36 58 GMVDCERIGT 0.150 586. 37 150 QVVEEIRQAN 0.150 587. 38 23 TKDVFQLKDL 0.120 588. 39 146 DCDPQVVEEI 0.120 589. 40 34 KIAPKEKGIT 0.100 590. 41 27 FQLKDLEKIA 0.100 591. 42 53 SLVDDGMVDC 0.100 592. 43 13 RTRMMEIFSE 0.100 593. 44 156 RQANKVAKEA 0.100 594. 45 154 EIRQANKVAK 0.100 595. 46 5 KGLSAEEKRT 0.100 596. 47 92 QLSEGSQKHA 0.100 597. 48 160 KVAKEAANRW 0.100 598. 49 64 RIGTSNYYWA 0.100 599. 50 129 SLRDQREQLK 0.100 600.

[0775] TABLE XVII (A) HLA PEPTIDE SCORING RESULTS-121P1F1-B35, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 36 AIPKEKGITA 12.000 601. 2 136 QLKAEVEKY 9.000 602. 3 161 VAKEAANRW 9.000 603. 4 129 SLRDQREQL 6.000 604. 5 46 SVKEVLQSL 6.000 605. 6 8 SAEEKRTRM 3.600 606. 7 166 ANRWTDNIF 3.000 607. 8 195 FGIPEDFDY 3.000 608. 9 43 TAMSVKEVL 3.000 609. 10 122 RLAKELSSL 3.000 610. 11 51 LQSLVDDGM 2.000 611. 12 193 RTFGIPEDF 2.000 612. 13 80 HARKHKLEV 1.800 613. 14 109 KAKIGRCET 1.800 614. 15 52 QSLVDDGMV 1.500 615. 16 1 MSKKKGLSA 1.500 616. 17 196 GIPEDFDYI 1.200 617. 18 165 AANRWTDNI 1.200 618. 19 66 GTSNYYWAF 1.000 619. 20 78 ALHARKHKL 1.000 620. 21 95 EGSQKHASL 1.000 621. 22 64 RIGTSNYYW 1.000 622. 23 34 KJAPKEKGI 0.800 623. 24 45 MSVKEVLQS 0.750 624. 25 57 DGMVDCERI 0.600 625. 26 120 RTRLAKELS 0.600 626. 27 13 RTRMMEIFS 0.600 627. 28 28 QLKDLEKIA 0.600 628. 29 27 FQLKDLEKI 0.600 629. 30 85 KLEVLESQL 0.600 630. 31 62 CERIGTSNY 0.600 631. 32 171 DNIFAIKSW 0.500 632. 33 35 IAPKEKGIT 0.450 633. 34 15 RMMEIFSET 0.400 634. 35 154 EIRQANKVA 0.300 635. 36 157 QANKVAKEA 0.300 636. 37 150 QVVEEIRQA 0.300 637. 38 115 CETEERTRL 0.300 638. 39 158 ANKVAKEAA 0.300 639. 40 164 EAANRWTDN 0.300 640. 41 81 ARKHKLEVL 0.300 641. 42 18 EIFSETKDV 0.300 642. 43 143 KYKDCDPQV 0.240 643. 44 42 ITAMSVKEV 0.200 644. 45 105 KSIEKAKIG 0.200 645. 46 74 FPSKALHAR 0.200 646. 47 148 DPQVVEEIR 0.200 647. 48 12 KRTRMMEIF 0.200 648. 49 24 KLDVQLKDL 0.200 649. 50 63 ERIGTSNYY 0.200 650.

[0776] TABLE XVIII (A)hz,1/51 !HLA PEPTIDE SCORING RESULTS-121P1F1-B35, 10-MERS? ? !? START? SUBSEQUENCE? SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION? SEQ.? ? !RANK? POSITION? RESIDUE LISTING? OF A MOLECULE CONTAINING THIS SUBSEQUENCE)? ID# 1 36 APKEKGITAM 240.000 651. 2 7 LSAEEKRTRM 20.000 652. 3 177 KSWAKRKFGF 10.000 653. 4 80 HARKHKLEVL 9.000 654. 5 77 KALHARKHKL 6.000 655. 6 45 MSVKEVLQSL 5.000 656. 7 128 SSLRDQREQL 5.000 657. 8 8 SAEEKRTRMM 3.600 658. 9 175 AIKSWAKRKF 3.000 659. 10 165 AANRWTDNIF 3.000 660. 11 135 EQLKAEVEKY 3.000 661. 12 20 FSETKDVFQL 2.250 662. 13 50 VLQSLVDDGM 2.000 663. 14 161 VAKEAANRWT 1.800 664. 15 103 LQKSIEKAKI 1.800 665. 16 132 DQREQLKAEV 1.200 666. 17 188 ENKIDRTFGI 1.200 667. 18 46 SVKEVLQSLV 1.200 668. 19 164 EAANRWTDNI 1.200 669. 20 65 IGTSNYYWAF 1.000 670. 21 42 ITAMSVKEVL 1.000 671. 22 160 KVAKEAANRW 1.000 672. 23 18 EIFSETKDVF 1.000 673. 24 114 RCETEERTRL 0.900 674. 25 120 RTRLAKELSS 0.600 675. 26 62 CERIGTSNYY 0.600 676. 27 61 DCERIGTSNY 0.600 677. 28 195 FGIPEDFDYI 0.600 678. 29 67 TSNYYWAFPS 0.500 679. 30 101 ASLQKSIEKA 0.500 680. 31 166 ANRWTDNIFA 0.450 681. 32 143 KYKDCDPQVV 0.360 682. 33 97 SQKHASLQKS 0.300 683. 34 58 GMVDCERIGT 0.300 684. 35 5 KGLSAEEKRT 0.300 685. 36 194 TFGIPEDFDY 0.300 686. 37 34 KIAPKEKGIT 0.300 687. 38 158 ANKVAKEAAN 0.300 688. 39 148 DPQVVEEIRQ 0.300 689. 40 11 EKRTRMMEIF 0.300 690. 41 112 IGRCETEERT 0.300 691. 42 35 IAPKEKGITA 0.300 692. 43 118 EERTRLAKEL 0.300 693. 44 157 QANKVAKEAA 0.300 694. 45 72 WAFPSKALHA 0.300 695. 46 51 LQSLVDDGMV 0.300 696. 47 105 KSIEKAKIGR 0.200 697. 48 64 RIGTSNYYWA 0.200 698. 49 74 FPSKALHARK 0.200 699. 50 150 QVVEEIRQAN 0.200 700.

[0777] TABLE V (B): VARIANT 1A KLE:VLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A1, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 98 FHEIJKVSY 4.500 701 2 88 VLESQDPGC 1.800 702 3 95 GCCFHEIIK 1.000 701. 4 91 SQDPGCCFH 0.750 702. 5 118 ACNPSTLGG 0.500 703. 6 90 ESQDPGCCF 0.150 704. 7 85 KLEVLESQD 0.090 705. 8 104 VSYYRKFWL 0.075 706. 9 96 CCFHEIIKV 0.050 707. 10 101 IIKVSYYRK 0.040 708. 11 99 HEIIKVSYY 0.025 709. 12 115 VAHACNPST 0.020 710. 13 100 EIIKVSYYR 0.020 711. 14 103 KVSYYRKFW 0.010 712. 15 117 HACNPSTLG 0.010 713. 16 111 WLGAVAHAC 0.010 714. 17 114 AVAHACNPS 0.010 715. 18 87 EVLESQDPG 0.010 716. 19 102 IKVSYYRKF 0.005 717. 20 112 LGAVAHACN 0.005 718. 21 93 DPGCCFHEI 0.003 719. 22 108 RKFWLGAVA 0.001 720. 23 110 FWLGAVAHA 0.001 721. 24 113 GAVAHACNP 0.001 722. 25 97 CFHEIIKVS 0.001 723. 26 116 AHACNPSTL 0.001 724. 27 89 LESQDPGCC 0.001 725. 28 92 QDPGCCFHE 0.000 726. 29 94 PGCCFHEII 0.000 727. 30 110 KFWLGAVAH 0.000 728. 31 105 SYYRKFWLG 0.000 729. 32 86 LEVLESQDP 0.000 730. 33 107 YRKFWLGAV 0.000 731. 34 106 YYRKFWLGA 0.000 732.

[0778] TABLE VI (B) VARIANT 1A HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A1, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 98 FHEIIKVSYY 2.250 735. 2 88 VLESQDPGCC 0.900 736. 3 91 SQDPGCCFHE 0.375 737. 4 85 KLEVLESQDP 0.090 738. 5 95 GCCFHEIIKV 0.005 739. 6 117 HACNPSTLGG 0.050 740. 7 97 CFHEIIKVSY 0.050 741. 8 103 KVSYYRKFWL 0.050 742. 9 100 EIIKVSYYRK 0.040 743. 10 94 PGCCFHEIIK 0.025 744. 11 111 WLGAVAHACN 0.020 745. 12 114 AVAHACNPST 0.020 746. 13 87 EVLESQDPGC 0.020 737. 14 90 ESQDPGCCFH 0.015 748. 15 104 VSYYRKFWLG 0.015 749. 16 113 GAVAHACNPS 0.010 750. 17 99 HEIIKVSYYR 0.010 751. 18 115 VAHACNPSTL 0.010 752. 19 101 IIKVSYYRKF 0.010 753. 20 96 CCFHEIIKVS 0.010 754. 21 89 LESQDPGCCF 0.005 755. 22 93 DPGCCFHEII 0.003 756. 23 108 RKFWLGAVAH 0.001 757. 24 92 QDPGCCFHEI 0.001 758. 25 116 AHACNPSTLG 0.001 759. 26 102 IKVSYYRKFW 0.001 760. 27 110 FWTGAVAHAC 0.001 761. 28 86 LEVLESQDPG 0.001 762. 29 105 SYYRKFWLGA 0.000 763. 30 112 LGAVAHACNP 0.000 764. 31 109 KFWLGAVAHA 0.000 765. 32 107 YRKFWLGAVA 0.000 766. 33 84 HKLEVLESQD 0.000 767. 34 106 YYRKFWLGAV 0.000 768.

[0779] TABLE VII (B) VARIANT 1A KLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A2, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 104 VSYYRKFWL 24.199 769. 2 111 WLGAVAHAC 22.853 770. 3 96 CCFHEIIKV 3.864 771. 4 88 VLESQDPGC 0.541 772. 5 115 VAHACNPST 0.176 773. 6 103 KVSYYRKFW 0.126 774. 7 110 FWLGAVAHA 0.027 775. 8 89 LESQDPGCC 0.021 776. 9 91 SQDPGCCFH 0.017 777. 10 116 AHACNPSTL 0.015 778. 11 108 RKFWLGAVA 0.010 779. 12 93 DPGCCFHEI 0.010 780. 13 114 AVAHACNPS 0.007 781. 14 87 EVLESQDPG 0.004 782. 15 85 KLEVLESQD 0.003 783. 16 106 YYRKFWLGA 0.002 784. 17 109 KFWLGAVAH 0.002 785. 18 94 PGCCFHEII 0.001 786. 19 100 EIIKVSYYR 0.001 787. 20 112 LGAVAHACN 0.001 788. 21 99 HEIIKVSYY 0.001 789. 22 86 LEVLESQDP 0.000 790. 23 118 ACNPSTLGG 0.000 791. 24 105 SYYRKFWLG 0.000 792. 25 107 YRKFWLGAV 0.000 793. 26 113 GAVAHACNP 0.000 794. 27 97 CFHEIIKVS 0.000 795. 28 101 IIKVSYYRK 0.000 796. 29 90 ESQDPGCCF 0.000 797. 30 92 QDPGCCFHE 0.000 798. 31 102 IKVSYYRKF 0.000 799. 32 95 GCCFHEIIK 0.000 800. 33 117 HACNPSTLG 0.000 801. 34 98 FHEIIKVSY 0.000 802.

[0780] TABLE VIII (B) VARIANT 1A: HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A2, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 103 KVSYYRKFWL 208.697 803. 2 95 GCCFHEIIKV 1.044 804. 3 114 AVAHACNPST 0.652 805. 4 115 VAHACNPSTL 0.504 806. 5 87 EVLESQDPGC 0.495 807. 6 111 WLGAVAHACN 0.343 808. 7 109 KFWLGAVAHA 0.231 809. 8 88 VLESQDPGCC 0.070 810. 9 104 VSYYRKFWLG 0.038 811. 10 92 QDPGCCFHEI 0.028 812. 11 105 SYYRKFWLGA 0.014 813. 12 110 FWLGAVAHAC 0.012 814. 13 93 DPGCCFHEII 0.004 815 14 91 SQDPGCCFHE 0.004 816. 15 85 KLEVLESQDP 0.003 817. 16 89 LESQDPGCCF 0.002 818. 17 96 CCFHEIIKVS 0.002 819. 18 86 LEVLESQDPG 0.001 820. 19 113 GAVAHACNPS 0.001 821. 20 106 YYRKFWLGAV 0.001 822. 21 102 IKVSYYRKFW 0.001 823. 22 90 ESQDPGCCFH 0.001 824. 23 108 RKFWLGAVAH 0.000 825. 24 100 EIIKVSYYRK 0.000 826. 25 97 CFHEIIKVSY 0.000 827. 26 98 FHEIIKVSYY 0.000 828. 27 101 HKVSYYVRKF 0.000 829. 28 112 LGAVAHACNP 0.000 830. 29 99 HEIIKVSYYR 0.000 831. 30 116 AHACNPSTLG 0.000 832. 31 107 YRKFWLGAVA 0.000 833. 32 117 HACNPSTLGG 0.000 834. 33 84 HKLEVLESQD 0.000 835. 34 94 PGCCFHEIIK 0.000 836.

[0781] TABLE IX (B) VARIANT 1A KLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A3, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION SEQ. RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) ID# 1 101 IIKVSYYRK 6.000 837. 2 95 GCCFHEIIK 1.200 838. 3 100 EIIKVSYYR 0.810 839. 4 111 WLGAVAHAC 0.300 840. 5 88 VLESQDPGC 0.200 841. 6 103 KVSYYRKFW 0.090 842. 7 85 KLEVLESQD 0.060 843. 8 99 HEIIKVSYY 0.054 844. 9 104 VSYYRKFWL 0.045 845. 10 96 CCFHEIIKV 0.030 846. 11 91 SQDPGCCFH 0.009 847. 12 98 FHEIIKVSY 0.006 848. 13 93 DPGCCFHEI 0.005 849. 14 90 ESQDPGCCF 0.005 850. 15 114 AVAHACNPS 0.004 851. 16 109 KFWLGAVAH 0.003 852. 17 87 EVLESQDPG 0.001 853. 18 110 FWLGAVAHA 0.001 854. 19 106 YYRKFWLGA 0.001 855. 20 115 VAHACNPST 0.001 856. 21 108 RKFWLGAVA 0.001 857. 22 102 IKVSYYRKF 0.001 858. 23 105 SYYRKFWLG 0.001 859. 24 113 GAVAHACNP 0.001 860. 25 118 ACNPSTLGG 0.001 861. 26 116 AHACNPSTL 0.001 862. 27 117 HACNPSTLG 0.000 863. 28 107 YRKFWLGAV 0.000 864. 29 94 PGCCFHEII 0.000 865. 30 89 LESQDPGCC 0.000 866. 31 92 QDPGCCFHE 0.000 867. 32 86 LEVLESQDP 0.000 868. 33 97 CFHEIIKVS 0.000 869. 34 112 LGAVAHACN 0.000 870.

[0782] TABLE X (B) VARIANT 1A HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A3, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 100 EIIKVSYYRK 2.700 871. 2 103 KVSYYRKFWL 0.540 872. 3 99 HEIIKVSYYR 0.081 873. 4 88 VLESQDPGCC 0.060 874. 5 85 KLEVLESQDP 0.060 875. 6 101 IIKVSYYRKF 0.060 876. 7 111 WLGAVAHACN 0.020 877. 8 95 GCCFHEIIKV 0.018 878. 9 88 EVLESQDPGC 0.013 879. 10 98 FHEIIKVSYY 0.012 880. 11 114 AVAHACNPST 0.010 881. 12 97 CFHEIIKVSY 0.009 882. 13 109 KFWLGAVAHA 0.009 883. 14 89 LESQDPGCCF 0.009 884. 15 105 SYYRKFWLGA 0.006 885. 16 115 VAHACNPSTL 0.006 886. 17 93 DPGCCFHEII 0.005 887. 18 104 VSYYRKFWLG 0.005 888. 19 94 PGCCFHEIIK 0.004 889. 20 91 SQDPGCCFHE 0.003 890. 21 92 QDPGCCFHEI 0.003 891. 22 96 CCFHEIIKVS 0.002 892. 23 113 GAVAHACNPS 0.002 893. 24 108 RKFWLGAVAH 0.001 894. 25 110 FWLGAVAHAC 0.001 895. 26 102 IKVSYYRKFW 0.000 896. 27 117 HACNPSTLGG 0.000 897. 28 90 ESQDPGCCFH 0.000 898. 29 106 YYRKFWLGAV 0.000 899. 30 107 YRKFWLGAVA 0.000 900. 31 86 LEVLESQDPG 0.000 901. 32 84 HKLEVLESQD 0.000 902. 33 33 AHACNPSTLG 0.000 903. 34 29 LGAVAHACNP 0.000 904.

[0783] TABLE XI (B) VARIANT 1A HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A11, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ ID# 1 95 GCCFHEIIK 1.200 905. 2 101 IIKVSYYRK 0.800 906. 3 100 EIIKVSYYR 0.072 907. 4 103 KVSYYRKFW 0.030 908. 5 109 KFWLGAVAH 0.012 909. 6 96 CCFHEIIKV 0.008 910. 7 106 YYRKFWLGA 0.008 911. 8 91 SQDPGCCFH 0.006 912. 9 114 AVAHACNPS 0.002 913. 10 105 SYYRKFWLG 0.002 914. 11 85 KLEVLESQD 0.001 915. 12 104 VSYYRKFWL 0.001 916. 13 108 RKFWLGAVA 0.001 917. 14 87 EVLESQDPG 0.001 918. 15 113 GAVAHACNP 0.001 919. 16 99 HEIIKVSYY 0.001 920. 17 93 DPGCCFHEI 0.001 921. 18 88 VLESQDPGC 0.000 922. 19 118 ACNPSTLGG 0.000 923. 20 111 WLGAVAHAC 0.000 924. 21 110 FWLGAVAHA 0.000 925. 22 116 AHACNPSTL 0.000 926. 23 98 FHEIIKVSY 0.000 927. 24 115 VAHACNPST 0.000 928. 25 117 HACNPSTLG 0.000 929. 26 107 YRKFWLGAV 0.000 930. 27 97 CFHEIIKVS 0.000 931. 28 86 LEVLESQDP 0.000 932. 29 92 QDPGCCFHE 0.000 933. 30 89 LESQDPGCC 0.000 934. 31 90 ESQDPGCCF 0.000 935. 32 102 IKVSYYRKF 0.000 936. 33 112 LGAVAHACN 0.000 937. 34 94 PGCCFHEII 0.000 938.

[0784] TABLE XII (B) VARIANT 1A HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A11, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ ID# 1 100 EIIKVSYYRK 0.360 939. 2 103 KVSYYRKFWL 0.180 940. 3 99 HEIIKVSYYR 0.036 941. 4 105 SYYRKFWLGA 0.016 942. 5 95 GCCFHEIIKV 0.012 943. 6 109 KFWLGAVAHA 0.012 944. 7 94 PGCCFHEIIK 0.004 945. 8 106 YYRKFWLGAV 0.00 4946. 9 97 CFHEIIKVSY 0.002 947. 10 114 AVAHACNPST 0.002 948. 11 115 VAHACNPSTL 0.002 949. 12 91 SQDPGCCFHE 0.002 950. 13 85 KLEVLESQDP 0.001 951. 14 108 RKFWLGAVAH 0.001 952. 15 113 GAVAHACNPS 0.001 953. 16 87 EVLESQDPGC 0.001 954. 17 93 DPGCCFHEII 0.001 955. 18 89 LESQDPGCCF 0.001 956. 19 101 IIKVSYYRKF 0.000 957. 20 111 WLGAVAHACN 0.000 958. 21 117 HACNPSTLGG 0.000 959. 22 88 VLESQDPGCC 0.000 960. 23 98 FHEIIKVSYY 0.000 961. 24 92 QDPGCCFHEI 0.000 962. 25 96 CCFHEIIKVS 0.000 963. 26 107 YRKFWLGAVA 0.000 964. 27 102 IKVSYYRKFW 0.000 965. 28 86 LEVLESQDPG 0.000 966. 29 104 VSYYRKFWLG 0.000 967. 30 90 ESQDPGCCFH 0.000 968. 31 84 HKLEVLESQD 0.000 969. 32 110 FWLGAVAHAC 0.000 970. 33 112 LGAVAHACNP 0.000 971. 34 116 AHACNPSTLG 0.000 972.

[0785] TABLE XIII (B) VARIANT 1A KLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A24, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 106 YYRKFWLGA 5.000 973. 2 104 VSYYRKFWL 4.000 974. 3 90 ESQDPGCCF 3.600 975. 4 93 DPGCCFHEI 1.320 976. 5 97 CFHEIIKVS 0.840 977. 6 105 SYYRKFWLG 0.600 978. 7 116 AHACNPSTL 0.400 979. 8 102 IKVSYYRKF 0.330 980. 9 103 KVSYYRKFW 0.200 981. 10 88 VLESQDPGC 0.150 982. 11 110 FWLGAVAHA 0.150 983. 12 111 WLGAVAHAC 0.140 984. 13 114 AVAHACNPS 0.120 985. 14 96 CCFHEIIKV 0.110 986. 15 112 LGAVAHACN 0.100 987. 16 115 VAHACNPST 0.100 988. 17 94 PGCCFHEII 0.100 989. 18 109 KLWLGAVAH 0.100 990. 19 85 KLEVLESQD 0.036 991. 20 108 RKFWLGAVA 0.024 992. 21 98 FHEIIKVSY 0.021 993. 22 100 EIIKVSYYR 0.021 994. 23 87 EVLESQDPG 0.018 995. 24 118 ACNPSTLGG 0.018 996. 25 113 GAVAHACNP 0.015 997. 26 99 HEIIKVSYY 0.015 998. 27 91 SQDPGCCFH 0.012 999. 28 101 IIKVSYYRK 0.010 1000 29 89 LESQDPGCC 0.010 1001 30 95 GCCFHEIIK 0.010 1002 31 117 HACNPSTLG 0.010 1003 32 107 YRKFWLGAV 0.010 1004 33 86 LEVLESQDP 0.002 1005 34 92 QDPGCCFHE 0.002 1006

[0786] TABLE XIV (B) VARIANT 1A HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-A24, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 103 KVSYYRKFWL 8.000 1007. 2 105 SYYRKFWLGA 5.000 1008. 3 106 YYRKFWLGAV 5.000 1009. 4 115 VAHACNPSTL 4.000 1010. 5 101 IIKVSYYRKF 2.200 1011. 6 93 DPGCCFHEII 1.000 1012. 7 109 KFWLGAVAHA 1.000 1013. 8 97 CFHEIIKVSY 0.840 1014. 9 110 FWLGAVAHAC 0.210 1015. 10 89 LESQDPGCCF 0.200 1016. 11 92 QDPGCCFHEI 0.198 1017. 12 87 EVLESQDPGC 0.180 1018. 13 113 GAVAHACNPS 0.180 1019. 14 88 VLESQDPGCC 0.150 1020. 15 96 CCFHEIIKVS 0.140 1021. 16 95 GCCFHEIIKV 0.110 1022. 17 114 AVAHACNPST 0.100 1023. 18 111 WLGAVAHACN 0.100 1024. 19 85 KLEVLESQDP 0.036 1025. 20 90 ESQDPGCCFH 0.018 1026. 21 100 EIIKVSYYRK 0.015 1027. 22 102 IKVSYYRKFW 0.015 1028. 23 98 FHEIIKVSYY 0.015 1029. 24 91 SQDPGCCFHE 0.012 1030. 25 104 VSYYRKFWLG 0.012 1031. 26 107 YRKFWLGAVA 0.012 1032. 27 112 LGAVAHACNP 0.010 1033. 28 117 HACNPSTLGG 0.010 1034. 29 84 HKLEVLESQD 0.002 1035. 30 99 HEIIKVSYYR 0.002 1036. 31 108 RKFWLGAVAH 0.002 1037. 32 86 LEVLESQDPG 0.002 1038. 33 94 PGCCFHEIIK 0.001 1039. 34 116 AHACNPSTLG 0.001 1040.

[0787] TABLE XV (B) VARIANT 1A KLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-B7, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 93 DPGCCFHEI 8.000 1041 2 104 VSYYRKFWL 4.000 1042 3 116 AHACNPSTL 1.200 1043 4 115 VAHACNPST 0.300 1044 5 114 AVAHACNPS 0.300 1045 6 96 CCFHEIIKV 0.200 1046 7 103 KVSYYRKFW 0.150 1047 8 111 WLGAVAHAC 0.100 1048 9 106 YYRKFWLGA 0.100 1049 10 87 EVLESQDPG 0.050 1050 11 117 HACNPSTLG 0.045 1051 12 94 PGCCFHEII 0.040 1052 13 113 GAVAHACNP 0.030 1053 14 90 ESQDPGCCF 0.030 1054 15 118 ACNPSTLGG 0.030 1055 16 88 VLESQDPGC 0.030 1056 17 107 YRKFWLGAV 0.020 1057 18 112 LGAVAHACN 0.020 1058 19 89 LESQDPGCC 0.010 1059 20 110 FWLGAVAHA 0.010 1060 21 108 RKFWLGAVA 0.010 1061 22 95 GCCFHEIIK 0.010 1062 23 101 IIKVSYYRK 0.010 1063 24 100 EIIKVSYYR 0.010 1064 25 85 KLEVLESQD 0.003 1065 26 91 SQDPGCCFH 0.003 1066 27 97 CFHEIIKVS 0.002 1067 28 102 IKVSYYRKF 0.002 1068 29 100 HEIIKVSYY 0.002 1069 30 109 KFWLGAVAH 0.001 1070 31 86 LEVLESQDP 0.001 1071 32 92 QDPGCCFHE 0.001 1072 33 105 SYYRKFWLG 0.001 1073 34 98 FHEIIKVSY 0.001 1074

[0788] TABLE XVI (B) VARIANT 1A HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-B7, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 103 KVSYYRKFWL 20.000 1075 2 115 VAHACNPSTL 12.000 1076 3 93 DPGCCFHEII 8.000 1077 4 114 AVAHACNPST 1.500 1078 5 87 EVLESQDPGC 0.500 1079 6 106 YYRKFWLGAV 0.200 1080 7 95 GCCFHEIIKV 0.200 1081 8 114 GAVAHACNPS 0.060 1082 9 92 QDPGCCFHEI 0.040 1083 10 117 HACNPSTLGG 0.030 1084 11 88 VLESQDPGCC 0.030 1085 12 96 CCFHEIIKVS 0.020 1086 13 101 IIKVSYYRKF 0.020 1087 14 111 WLGAVAHACN 0.020 1088 15 110 FWLGAVAHAC 0.010 1089 16 107 YRKFWLGAVA 0.010 1090 17 105 SYYRKFWLGA 0.010 1091 18 104 VSYYRKFWLG 0.010 1092 19 109 KFWLGAVAHA 0.010 1093 20 100 EIIKVSYYRK 0.010 1094 21 90 ESQDPGCCFH 0.010 1095 22 112 LGAVAHACNP 0.010 1096 23 116 AHACNPSTLG 0.005 1097 24 102 IKVSYYRKFW 0.003 1098 25 89 LESQDPGCCF 0.003 1099 26 91 SQDPGCCFHE 0.003 1100 27 85 KLEVLESQDP 0.003 1101 28 97 CFHEIIKVSY 0.002 1102 29 108 RKFWLGAVAH 0.001 1103 30 94 PGCCFHEIIK 0.001 1104 31 86 LEVLESQDPG 0.001 1105 32 99 HEIIKVSYYR 0.001 1106 33 84 HKLEVLESQD 0.001 1107 34 98 FHEIIKVSYY 0.001 1108

[0789] TABLE XVII (B) VARIANT 1A KLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-B35, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 90 ESQDPGCCF 10.000 1109 2 93 DPGCCFHEI 8.000 1110 3 104 VSYYRKFWL 5.000 1111 4 103 KVSYYRKFW 1.000 1112 5 115 VAHACNPST 0.300 1113 6 96 CCFHEIIKV 0.300 1114 7 99 HEIIKVSYY 0.200 1115 8 112 LGAVAHACN 0.100 1116 9 111 WLGAVAHAC 0.100 1117 10 114 AVAHACNPS 0.100 1118 11 116 AHACNPSTL 0.100 1119 12 102 IKVSYYRKF 0.100 1120 13 107 YRKFWLGAV 0.060 1121 14 98 FHEIIKVSY 0.060 1122 15 94 PGCCFHEII 0.040 1123 16 117 HACNPSTLG 0.030 1124 17 106 YYRKFWLGA 0.030 1125 18 101 IIKVSYYRK 0.030 1126 19 113 GAVAHACNP 0.030 1127 20 88 VLESQDPGC 0.030 1128 21 87 EVLESQDPG 0.020 1129 22 97 CFHEIIKVS 0.020 1130 23 108 RKFWLGAVA 0.020 1131 24 89 LESQDPGCC 0.015 1132 25 95 GCCFHEIIK 0.010 1133 26 118 ACNPSTLGG 0.010 1134 27 110 FWLGAVAHA 0.010 1135 28 100 EIIKVSYYR 0.010 1136 29 85 KLEVLESQD 0.006 1137 30 91 SQDPGCCFH 0.003 1138 31 109 KFWLGAVAH 0.002 1139 32 86 LEVLESQDP 0.002 1140 33 92 QDPGCCFHE 0.001 1141 34 105 SYYRKFWLG 0.001 1142

[0790] TABLE XVIII (B) VARIANT 1A HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG HLA PEPTIDE SCORING RESULTS-121P1F1-B35, 10-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 93 DPGCCFHEII 8.000 1143 2 115 VAHACNPSTL 3.000 1144 3 101 IIKVSYYRKF 3.000 1145 4 103 KVSYYRKFWL 2.000 1146 5 97 CFHEIIKVSY 0.400 1147 6 113 GAVAHACNPS 0.300 1148 7 95 GCCFHEIIKV 0.300 1149 8 87 EVLESQDPGC 0.200 1150 9 114 AVAHACNPST 0.100 1151 10 89 LESQDPGCCF 0.100 1152 11 90 ESQDPGCCFH 0.100 1153 1153 12 111 WLGAVAHACN 0.100 1154 13 96 CCFHEIIKVS 0.100 1155 14 106 YYRKFWLGAV 0.060 1156 15 98 FHEIIKVSYY 0.060 1157 16 102 IKVSYYRKFW 0.05 01158 17 104 VSYYRKFWLG 0.05 01159 18 88 VLESQDPGCC 0.045 1160 19 92 QDPGCCFHEI 0.040 1161 20 107 YRKFWLGAVA 0.030 1162 21 117 HACNPSTLGG 0.030 1163 22 109 KFWLGAVAHA 0.020 1164 23 105 SYYRKFWLGA 0.010 1165 24 110 FWLGAVAHAC 0.010 1166 25 112 LGAVAHACNP 0.010 1167 26 100 EIIKVSYYRK 0.010 1168 27 85 KLEVLESQDP 0.009 1169 28 91 SQDPGCCFHE 0.003 1170 29 108 RKFWLGAVAH 0.002 1171 30 84 HKLEVLESQD 0.002 1172 31 86 LEVLESQDPG 0.001 1173 32 116 AHACNPSTLG 0.001 1174 33 94 PGCCFHEIIK 0.001 1175 34 99 HEIIKVSYYR 0.001 1176

[0791] TABLE V (C) VARIANT 1B MKCKMELSEGSQKH HLA PEPTIDE SCORING RESULTS-121P1F1-A1, 9-MERS SCORE START SUBSEQUENCE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ ID# 1 4 KMELSEGSQ 0.450 1177 2 5 MELSEGSQK 0.010 1178. 3 6 ELSEGSQKH 0.010 1179. 4 2 KCKMELSEG 0.001 1180. 5 3 CKMELSEGS 0.001 1181. 6 1 MKCKMELSE 0.000 1182.

[0792] TABLE VI (C) VARIANT 1B MKCKMELSEGSQKHA HLA PEPTIDE SCORING RESULTS—121P1F1-A1, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 4 KMELSEGSQK 9.000 1183. 2 6 ELSEGSQKHA 0.010 1184. 3 2 KCKMELSEGS 0.001 1185. 4 5 MELSEGSQKH 0.001 1186. 5 3 CKMELSEGSQ 0.001 1187. 6 1 MKCKMELSEG 0.000 1188.

[0793] TABLE VII (C) VARIANT 1B MKCKMELSEGSQKH HLA PEPTIDE SCORING RESULTS—121P1F1-A2, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 6 ELSEGSQKH 0.023 1189. 2 5 MELSEGSQK 0.002 1190. 3 3 CKMELSEGS 0.001 1191. 4 4 KMELSEGSQ 0.000 1192. 5 2 KCKMELSEG 0.000 1193. 6 1 MKCKMELSE 0.000 1194.

[0794] TABLE VIII (C) VARIANT 1B MKCKMELSEGSQKHA HLA PEPTIDE SCORING RESULTS—121P1F1-A2, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 6 ELSEGSQKHA 1.528 1195. 2 5 MELSEGSQKH 0.009 1196. 3 4 KMELSEGSQK 0.002 1197. 4 1 MKCKMELSEG 0.000 1198. 5 3 CKMELSEGSQ 0.000 1199. 6 2 KCKMELSEGS 0.000 1200.

[0795] TABLE IX (C) VARIANT 1B MKCKMELSEGSQKH HLA PEPTIDE SCORING RESULTS—121P1F1-A3, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 6 ELSEGSQKH 0.090 1201. 2 5 MELSEGSQK 0.090 1202. 3 4 KMELSEGSQ 0.018 1203. 4 2 KCKMELSEG 0.001 1204. 5 3 CKMELSEGS 0.000 1205. 6 1 MKCKMELSE 0.000 1206.

[0796] TABLE X (C) VARIANT 1B MKCKMELSEGSQKHA HLA PEPTIDE SCORING RESULTS—121P1F1-A3, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ. ID# 1 4 KMELSEGSQK 60.000 1207. 2 6 ELSEGSQKHA 0.045 1208. 3 2 KCKMELSEGS 0.001 1209. 4 5 MELSEGSQKH 0.001 1210. 5 1 MKCKMELSEG 0.000 1211. 6 3 CKMELSEGSQ 0.000 1212.

[0797] TABLE XI (C) VARIANT 1B MKCKMELSEGSQKH HLA PEPTIDE SCORING RESULTS—121P1F1-A11, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 5 MELSEGSQK 0.090 1213. 2 4 KMELSEGSQ 0.001 1214. 3 6 ELSEGSQKH 0.001 1215. 4 2 KCKMELSEG 0.001 1216. 5 3 CKMELSEGS 0.000 1217. 6 1 MKCKMELSE 0.000 1218.

[0798] TABLE XII (C) VARIANT 1B MKCKMELSEGSQKHA HLA PEPTIDE SCORING RESULTS—121P1F1-A11, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 4 KMELSEGSQK 1.200 1219. 2 5 MELSEGSQKH 0.001 1220. 3 2 KCKMELSEGS 0.001 1221. 4 6 ELSEGSQKHA 0.001 1222. 5 3 CKMELSEGSQ 0.000 1223. 6 1 MKCKMELSEG 0.000 1224.

[0799] TABLE XIII (C) VARIANT 1B MKCKMELSEGSQKH HLA PEPTIDE SCORING RESULTS—121P1F1-A24, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 4 KMELSEGSQ 0.030 1225. 2 2 KCKMELSEG 0.022 1226. 3 3 CKMELSEGS 0.022 1227. 4 6 ELSEGSQKH 0.016 1228. 5 5 MELSEGSQK 0.002 1229. 6 1 MKCKMELSE 0.001 1230.

[0800] TABLE XIV (C) VARIANT 1B MKCKMELSEGSQKHA HLA PEPTIDE SCORING RESULTS—121P1F1-A24, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 2 KCKMELSEGS 0.240 1231. 2 6 ELSEGSQKHA 0.120 1232. 3 4 KMELSEGSQK 0.030 1233. 4 5 MELSEGSQKH 0.002 1234. 5 3 CKMELSEGSQ 0.002 1235. 6 1 MKCKMELSEG 0.001 1236.

[0801] TABLE XV (C) VARIANT 1B MKCKMELSEGSQKH HLA PEPTIDE SCORING RESULTS—121P1F1-B7, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 6 ELSEGSQKH 0.010 1237. 2 2 KCKMELSEG 0.010 1238. 3 3 CKMELSEGS 0.006 1239. 4 4 KMELSEGSQ 0.003 1240. 5 5 MELSEGSQK 0.001 1241. 6 1 MKCKMELSE 0.001 1242.

[0802] TABLE XVI (C) VARIANT 1B MKCKMELSEGSQKHA HLA PEPTIDE SCORING RESULTS-121P1F1-B7, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 6 ELSEGSQKHA 0.100 1243. 2 2 KCKMELSEGS 0.020 1244. 3 3 CKMELSEGSQ 0.003 1245. 4 4 KMELSEGSQK 0.003 1246. 5 5 MELSEGSQKH 0.001 1247. 6 1 MKCKMELSEG 0.001 1248.

[0803] TABLE XVII (C) VARIANT 1B MKCKMELSEGSQKH HLA PEPTIDE SCORING RESULTS-121P1F1-B35, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 2 KCKMELSEG 0.090 1249. 2 6 ELSEGSQKH 0.020 1250. 3 3 CKMELSEGS 0.020 1251. 4 4 KMELSEGSQ 0.006 1252. 5 5 MELSEGSQK 0.002 1253. 6 1 MKCKMELSE 0.001 1254.

[0804] TABLE XVIII (C) VARIANT 1B MKCKMELSEGSQKHA HLA PEPTIDE SCORTNG RESULTS-121P1F1-B35, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 2 KCKMELSEGS 0.600 1255. 2 6 ELSEGSQKHA 0.200 1256. 3 4 KMELSEGSQK 0.009 1257. 4 3 CKMELSEGSQ 0.002 1258. 5 1 MKCKMELSEG 0.002 1259. 6 5 MELSEGSQKH 0.001 1260.

[0805] TABLE V (D): VARIANT 2 AKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS-121P1F1-A1, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 114 RCETAKQIK 18.000 1261 2 111 KIGRCETAK 0.020 1183. 3 113 GRCETAKQI 0.001 1184. 4 112 IGRCETAKQ 0.001 1185. 5 110 AKIGRCETA 0.001 1186.

[0806] TABLE VI (D) VARIANT 2 KAKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS-121P1F1-A1, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 113 GRCETAKQIK 0.010 1261. 2 110 AKIGRCETAK 0.010 1262. 3 111 KIGRCETAKQ 0.002 1263. 4 109 KAKIGRCETA 0.001 1264. 5 112 IGRCETAKQI 0.000 1265.

[0807] TABLE VII (D) VARIANT 2 AKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS-121P1F1-A2, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 111 KIGRCETAK 0.007 1266. 2 113 GRCETAKQI 0.006 1267. 3 110 AKIGRCETA 0.003 1268. 4 112 IGRCETAKQ 0.000 1269. 5 114 RCETAKQIK 0.000 1270.

[0808] TABLE VIII (D) VARIANT 2 KAKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS-121P1F1-A2, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 112 IGRCETAKQI 0.009 1271. 2 111 KIGRCETAKQ 0.007 1272. 3 109 KAKIGRCETA 0.004 1273. 4 110 AKIGRCETAK 0.000 1274. 5 113 GRCETAKQIK 0.000 1275.

[0809] TABLE IX (D) VARIANT 2 AKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS-121P1F1-A3, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 111 KIGRCETAK 6.000 1276. 2 114 RCETAKQIK 0.200 1277. 3 113 GRCETAKQI 0.001 1278. 4 110 AKIGRCETA 0.000 1279. 5 112 IGRCETAKQ 0.000 1280.

[0810] TABLE X (D) VARIANT 2 KAKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS-121P1F1-A3, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 113 GRCETAKQIK 0.090 1281. 2 110 AKIGRCETAK 0.045 1282. 3 111 KIGRCETAKQ 0.006 1283. 4 109 KAKIGRCETA 0.006 1284. 5 112 IGRCETAKQI 0.000 1285.

[0811] TABLE XI (D) VARIANT 2 AKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS-121P1F1-A11, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 111 KIGRCETAK 1.200 1286. 2 114 RCETAKQIK 0.600 1287. 3 110 AKIGRCETA 0.000 1288. 4 113 GRCETAKQI 0.000 1289. 5 112 IGRCETAKQ 0.000 1290.

[0812] TABLE XII (D) VARIANT 2 KAKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS—121P1F1-A11, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 113 GRCETAKQIK 0.060 1291. 2 110 AKIGRCETAK 0.030 1292. 3 109 KAKIGRCETA 0.006 1293. 4 111 KIGRCETAKQ 0.001 1294. 5 112 IGRCETAKQI 0.000 1295.

[0813] TABLE XIII (D) VARIAINT 2 AKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS—121P1F1-A24, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 113 GRCETAKQI 0.120 1296. 2 114 RCETAKQIK 0.036 1297. 3 111 KIGRCETAK 0.020 1298. 4 110 AKIGRCETA 0.015 1299. 5 112 IGRCETAKQ 0.011 1300.

[0814] TABLE XIV (D) VARIANT 2 KAKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS—121P1F1-A24, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 112 IGRCETAKQI 1.000 1301. 2 109 KAKIGRCETA 0.200 1302. 3 111 KIGRCETAKQ 0.022 1303. 4 110 AKIGRCETAK 0.002 1304. 5 113 GRCETAKQIK 0.001 1305.

[0815] TABLE XV (D) VARIANT 2 AKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS—121P1F1-B7, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 112 IGRCETAKQ 0.100 1306. 2 113 GRCETAKQI 0.040 1307. 3 110 AKIGRCETA 0.030 1308. 4 111 KIGRCETAK 0.010 1309. 5 114 RCETAKQIK 0.003 1310.

[0816] TABLE XVI (D) VARIANT 2 KAKIGRCETAKQIK HLA PEPTIDE SCORING RESULT—121P1F1-B7, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 112 IGRCETAKQI 4.000 1311. 2 109 KAKIGRCETA 0.300 1312. 3 111 KIGRCETAKQ 0.010 1313. 4 110 AKIGRCETAK 0.003 1314. 5 113 GRCETAKQIK 0.001 1315.

[0817] TABLE XVII (D) VARIANT 2 AKIGRCETAKQIK HLA PEPTIDE SCORING RESULTS—121P1F1-B35, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 113 GRCETAKQI 0.080 1316. 2 112 IGRCETAKQ 0.045 1317. 3 111 KIGRCETAK 0.020 1318. 4 110 AKIGRCETA 0.010 1319. 5 114 RCETAKQIK 0.006 1320.

[0818] TABLE XVIII (D) VARIANT 2 KAKIGRCETAKQIK hl,53 HLA PEPTIDE SCORING RESULTS—121P1F1-B35, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 109 KAKIGRCETA 1.800 1321. 2 112 IGRCETAKQI 1.200 1322. 3 111 KIGRCETAKQ 0.030 1323. 4 113 GRCETAKQIK 0.002 1324. 5 110 AKIGRCETAK 0.001 1325.

[0819] TABLE V (E) VARIANT 3 DPQVVEEIHNIFAIKSW HLA PEPTIDE SCORING RESULTS—121P1F1-A1, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 151 VVEEIHNIF 9.000 1331. 2 154 EIHNIFAIK 0.400 1332. 3 152 VEEIHNIFA 0.225 1333. 4 151 QVVEEIHNI 0.010 1334. 5 155 IHNIFAIKS 0.003 1335. 6 156 HNIFAIKSW 0.003 1336. 7 153 EEIHNIFAI 0.003 1337. 8 148 DPQVVEEIH 0.003 1338. 9 149 PQVVEEIHN 0.001 1339.

[0820] TABLE VI (E) VARIANT 3 CDPQVVEEIHNIFAIKSWA HLA PEPTIDE SCORING RESULTS—121P1F1-A1, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 151 VVEEIHNIFA 4.500 1177. 2 152 VEEIHNIFAI 0.225 1178. 3 150 QVVEEIHNIF 0.100 1179. 4 154 EIHNIFAIKS 0.050 1180. 5 153 EEIHNIFAIK 0.020 1181. 6 148 DPQVVEEIHN 0.013 1182. 7 156 HNIFAIKSWA 0.003 1183. 8 155 IHNIFAIKSW 0.001 1184. 9 147 CDPQVVEEIH 0.001 1185. 10 149 PQVVEEIHNI 0.000 1186.

[0821] TABLE VII (E) VARIANT 3 DPQVVEEIHNIFAIKSW HLA PEPTIDE SCORING RESULTS—121P1F1-A2, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 150 QVVEEIHNI 8.608 1187. 2 153 EEIHNIFAI 0.203 1188. 3 152 VEEIHNIFA 0.058 1189. 4 151 VVEEIHNIF 0.001 1190. 5 155 IHNIFAIKS 0.000 1191. 6 149 PQVVEEIHN 0.000 1192. 7 154 EIHNIFAIK 0.000 1193. 8 156 HNIFAIKSW 0.000 1194. 9 148 DPQVVEEIH 0.000 1195.

[0822] TABLE VIII (E) VARIANT 3 CDPQVVEEIHNIFAIKSWA HLA PEPTIDE SCORING RESULTS—121P1F1-A2, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 151 VVEEIHNIFA 1.067 1196. 2 152 VEEIHNIFAI 0.294 1197. 3 149 PQVVEEIHNI 0.054 1198. 4 150 QVVEEIHNIF 0.011 1199. 5 156 HNIFAIKSWA 0.006 1200. 6 154 EIHNIFAIKS 0.003 1201. 7 155 IHNIFAIKSW 0.000 1202. 8 148 DPQVVEEIHN 0.000 1203. 9 147 CDPQVVEEIH 0.000 1204. 10 153 EEIHNIFAIK 0.000 1205.

[0823] TABLE IX (E) VARIANT 3 DPQVVEEIHNIFAIKSW HLA PEPTIDE SCORING RESULTS—121P1F1-A3, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 154 EIHNIFAIK 2.700 1206. 2 151 VVEEIHNIF 0.450 1207. 3 150 QVVEEIHNI 0.203 1208. 4 153 EEIHNIFAI 0.004 1209. 5 152 VEEIHNIFA 0.001 1210. 6 148 DPQVVEEIH 0.001 1211. 7 156 HNIFAIKSW 0.000 1212. 8 155 IHNIFAIKS 0.000 1213. 9 149 PQVVEEIHN 0.000 1214.

[0824] TABLE X (E) VARIANT 3 CDPQVVEEIHNIFAIKSWA HLA PEPTIDE SCORING RESULTS—121P1F1-A3, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 150 QVVEEIHNIF 0.675 1215. 2 153 EEIHNIFAIK 0.122 1216. 3 151 VVEEIHNIFA 0.060 1217. 4 152 VEEIHNIFAI 0.008 1218. 5 154 EIHNIFAIKS 0.007 1219. 6 149 PQVVEEIHNI 0.004 1220. 7 156 HNIFAIKSWA 0.001 1221. 8 147 CDPQVVEEIH 0.000 1222. 9 155 IHNIFAIKSW 0.000 1223. 10 148 DPQVVEEIHN 0.000 1224.

[0825] TABLE XII (E) VARIANT 3 DPQVVEEIHNIFAIKSW HLA PEPTIDE SCORING RESULTS—121P1F1-A11, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 154 EIHNIFAIK 0.120 1225. 2 150 QVVEEIHNI 0.030 1226. 3 151 VVEEIHNIF 0.020 1227. 4 152 VEEIHNIFA 0.001 1228. 5 153 EEIHNIFAI 0.001 1229. 6 148 DPQVVEEIH 0.001 1230. 7 156 HNIFAIKSW 0.000 1231. 8 149 PQVVEEIHN 0.000 1232. 9 155 IHNIFAIKS 0.000 1233.

[0826] TABLE XII (E) VARIANT 3 CDPQVVEEIHNIFAIKSWA HLA PEPTIDE SCORING RESULTS—121P1F1-A11, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 151 VVEEIHNIFA 0.040 1234. 2 150 QVVEEIHNIF 0.030 1235. 3 153 EEIHNIFAIK 0.027 1236. 4 152 VEEIHNIFAI 0.002 1237. 5 149 PQVVEEIHNI 0.001 1238. 6 156 HNIFAIKSWA 0.001 1239. 7 154 EIHNIFAIKS 0.000 1240. 8 147 CDPQVVEEIH 0.000 1241. 9 148 DPQVVEEIHN 0.000 1242. 10 155 IHNIFAIKSW 0.000 1243.

[0827] TABLE XIII (E) VARIANT 3 DPQVVEEIHNIFAIKSW HLA PEPTIDE SCORING RESULTS—121P1F1-A24, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 151 VVEEIHNIF 6.048 1244. 2 150 QVVEEIHNI 1.800 1245. 3 156 HNIFAIKSW 0.150 1246. 4 153 EEIHNIFAI 0.150 1247. 5 148 DPQVVEEIH 0.021 1248. 6 154 EIHNIFAIK 0.017 1249. 7 155 IHNIFAIKS 0.017 1250. 8 149 PQVVEEIHN 0.015 1251. 9 152 VEEIHNIFA 0.015 1252.

[0828] TABLE XIV (E) VARIANT 3 CDPQVVEEIHNIFAIKSWA HLA PEPTIDE SCORING RESULTS—121P1F1-A24, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 150 QVVEEIHNIF 6.048 1253. 2 156 HNIFAIKSWA 0.210 1254. 3 151 VVEEIHNIFA 0.180 1255. 4 148 DPQVVEEIHN 0.150 1256. 5 149 PQVVEEIHNI 0.150 1257. 6 152 VEEIHNIFAI 0.150 1258. 7 154 EIHNIFAIKS 0.110 1259. 8 155 IHNIFAIKSW 0.015 1260. 9 153 EEIHNIFAIK 0.003 1261. 10 147 CDPQVVEEIH 0.002 1262.

[0829] TABLE XV (E) VARIANT 3 DPQVVEEIHNIFAIKSW HLA PEPTIDE SCORING RESULTS—121P1F1-B7, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 150 QVVEEIHNI 2.000 1263. 2 148 DPQVVEEIH 0.200 1264. 3 153 EEIHNIFAI 0.040 1265. 4 151 VVEEIHNIF 0.030 1266. 5 156 HNIFAIKSW 0.020 1267. 6 154 EIHNIFAIK 0.010 1268. 7 152 VEEIHNIFA 0.003 1269. 8 149 PQVVEEIHN 0.002 1270. 9 155 IHNIFAIKS 0.002 1271.

[0830] TABLE XVI (E) VARIANT 3 CDPQVVEEIHNIFAIKSWA HLA PEPTIDE SCORING RESULTS—121P1F1-B7, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 148 DPQVVEEIHN 0.400 1272. 2 151 VVEEIHNIFA 0.150 1273. 3 150 QVVEEIHNIF 0.100 1274. 4 156 HNIFAIKSWA 0.100 1275. 5 149 PQVVEEIHNI 0.040 1276. 6 154 EIHNIFAIKS 0.020 1277. 7 152 VEEIHNIFAI 0.012 1278. 8 155 IHNIFAIKSW 0.002 1279. 9 153 EEIHNIFAIK 0.001 1280. 10 147 CDPQVVEEIH 0.001 1281.

[0831] TABLE XVII (E) VARIANT 3 DPQVVEEIHNIFAIKSW HLA PEPTIDE SCORING RESULTS—121P1F1-B35, 9-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 150 QVVEEIHNI 1.200 1282. 2 151 VVEEIHNIF 0.600 1283. 3 156 HNIFAIKSW 0.500 1284. 4 148 DPQVVEEIH 0.200 1285. 5 153 EEIHNIFAI 0.040 1286. 6 149 PQVVEEIHN 0.015 1287. 7 154 EIHNIFAIK 0.010 1288. 8 155 IHNIFAIKS 0.010 1289. 9 152 VEEIHNIFA 0.003 1290.

[0832] TABLE XVIII (E) VARIANT 3 CDPQVVEEIHNIFAIKSWA HLA PEPTIDE SCORING RESULTS-121P1F1-B35, 10-MERS START SUBSEQUENCE SCORE (ESTIMATE OF HALF TIME OF DISASSOCIATION RANK POSITION RESIDUE LISTING OF A MOLECULE CONTAINING THIS SUBSEQUENCE) SEQ.ID# 1 148 DPQVVEEIHN 3.000 1291. 2 150 QVVEEIHNIF 2.000 1292. 3 154 EIHNIFAIKS 0.100 1293. 4 156 HNIFAIKSWA 0.100 1294. 5 149 PQVVEEIHNI 0.060 1295. 6 151 VVEEIHNIFA 0.060 1296. 7 155 IHNIFAIKSW 0.050 1297. 8 152 VEEIHNIFAI 0.012 1298. 9 153 EEIHNIFAIK 0.001 1299. 10 147 CDPQVVEEIH 0.001 1300.

[0833] TABLE XIX Motifs and Post-translational Modifications of 121P1F1 Protein kinase C phosphorylation site Number of matches: 4 1  2-4 SKK 2  46-48 SVK 3  97-99 SQK 4 129-131 SLR Casein kinase II phosphorylation site Number of matches: 4 1  8-11 SAEE 2  46-49 SVKE 3  53-56 SLVD 4 129-132 SLRD N-myristoylation site  58-63 GMVDCE

[0834] TABLE XX Frequently Occurring Motifs avrg. % Name identity Description Potential Function zf-C2H2 34% Zinc finger, C2H2 type Nucleic acid-binding protein functions as transcription factor, nuclear location probable cytochrome_b_N 68% Cytochrome b(N- membrane bound oxidase, generate terminal)/b6/petB superoxide ig 19% Immunoglobulin domain domains are one hundred amino acids long and include a conserved intradomain disulfide bond. WD40 18% WD domain, G-beta repeat tandem repeats of about 40 residues, each containing a Trp-Asp motif. Function in signal transduction and protein interaction PDZ 23% PDZ domain may function in targeting signaling molecules to sub-membranous sites LRR 28% Leucine Rich Repeat short sequence motifs involved in protein- protein interactions pkinase 23% Protein kinase domain conserved catalytic core common to both serine/threonine and tyrosine protein kinases containing an ATP binding site and a catalytic site PH 16% PH domain pleckstrin homology involved in intracellular signaling or as constituents of the cytoskeleton EGF 34% EGF-like domain 30-40 amino-acid long found in the extracellular domain of membrane-bound proteins or in secreted proteins rvt 49% Reverse transcriptase (RNA-dependent DNA polymerase) ank 25% Ank repeat Cytoplasmic protein, associates integral membrane proteins to the cytoskeleton oxidored_q1 32% NADH- membrane associated. Involved in proton Ubiquinone/plastoquinone translocation across the membrane (complex I), various chains efhand 24% EF hand calcium-binding domain, consists of a 12 residue loop flanked on both sides by a 12 residue alpha-helical domain rvp 79% Retroviral aspartyl protease Aspartyl or acid proteases, centered on a catalytic aspartyl residue Collagen 42% Collagen triple helix repeat extracellular structural proteins involved in (20 copies) formation of connective tissue. The sequence consists of the G-X-Y and the polypeptide chains forms a triple helix. fn3 20% Fibronectin type III domain Located in the extracellular ligand-binding region of receptors and is about 200 amino acid residues long with two pairs of cysteines involved in disulfide bonds 7tm_1 19% 7 transmembrane receptor seven hydrophobic transmembrane regions, (rhodopsin family) with the N-terminus located extracellularly while the C-terminus is cytoplasmic. Signal through G proteins

[0835] TABLE XXI Properties of 121P1F1 Bioinformatic Program URL Outcome 121P1F1 ORF ORF finder 618 bp Protein length 205 aa Transmembrane region TM Pred http://www.ch.embnet.org/ no TM HMMTop http://www.enzim.hu/hmmtop/ no TM, intracellular Sosui http://www.genome.ad.jp/SOSui/ no TM, soluble protein TMHMM http://www.cbs.dtu.dk/services/TMHMM no TM Signal Peptide Signal P http://www.cbs.dtu.dk/services/SignalP/ none pI pI/MW tool http://www.expasy.ch/tools/ 8.28 Molecular weight pI/MW tool http://www.expasy.ch/tools/  23.7 kDa Localization PSORT http://psort.nibb.ac.jp/ 30% nuclear, 10% mitochondrial PSORT II http://psort.nibb.ac.jp/ 65% nuclear, 17% cytoplasmic Motifs Pfam http://www.sanger.ac.uk/Pfam/ Basic Zipper motif, Myc leucine zipper Prints http://www.biochem.ucl.ac.uk/ Steroid hormone receptor signature Blocks http://www.blocks.fhcrc.org/ no significant motif Variant 1A ORF ORF finder 618 bp Protein length 126 aa Transmembrane region TM Pred http://www.ch.embnet.org/ no TM HMMTop http://www.enzim.hu/hmmtop/ no TM, extracellular Sosui http://www.genome.ad.jp/SOSui/ no TM, soluble protein TMHMM http://www.cbs.dtu.dk/services/TMHMM no TM Signal Peptide Signal P http://www.cbs.dtu.dk/services/SignalP/ none pI pI/MW tool http://www.expasy.ch/tools/ 8.65 Molecular weight pI/MW tool http://www.expasy.ch/tools/  14.3 kDa Localization PSORT http://psort.nibb.ac.jp/ 30% nuclear, 11% peroxisome PSORT II http://psort.nibb.ac.jp/ 30% nuclear, 52.2% cytoplasmic Motifs Pfam http://www.sanger.ac.uk/Pfam/ no significant motif Prints http://www.biochem.ucl.ac.uk/ no significant motif Blocks http://www.blocks.fhcrc.org/ no significant motif Variant 4 ORF ORF finder 618 bp Protein length 190 aa Transmembrane region TM Pred http://www.ch.embnet.org/ no TM HMMTop http://www.enzim.hu/hmmtop/ no TM, intracellular Sosui http://www.genome.ad.jp/SOSui/ no TM, soluble protein TMHMM http://www.cbs.dtu.dk/services/TMHMM no TM Signal Peptide Signal P http://www.cbs.dtu.dk/services/SignalP/ none pI pI/MW tool http://www.expasy.ch/tools/ 6.05 Molecular weight pI/MW tool http://www.expasy.ch/tools/ 22.02 kDa Localization PSORT http://psort.nibb.ac.jp/ 30% nuclear, 10% mitochondrial matrix space, 10% lysosome PSORT II http://psort.nibb.ac.jp/ 65.2% nuclear, 21.7% mitochondrial, 13% cytoplasmic Motifs Pfam http://www.sanger.ac.uk/Pfam/ bZip transcription factor Myc leucine zipper Prints http://www.biochem.ucl.ac.uk/ steroid hormone receptor signature Blocks http://www.blocks.fhcrc.org/ no significant motif

[0836] TABLE XXIIA Nucleotide sequence of splice variant 1. ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga aqaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcaggac 360 cctggctgct gcttccatga aataattaaa gtctcctatt atagaaaatt ctggctgggc 420 gcagtggctc acgcctgtaa tcccagcact ttgggaggct gaggcgggca gatcacgagg 480 tgactttccc ccacccccac atgaagtgca agatggagtt gtctgaggga agtcaaaagc 540 atgcaagcct acagaaaagc attgagaaag ctaaaattgg ccgatgtgaa acggaagagc 600 gaaccaggct agcaaaagag ctttcttcac ttcgagacca aagggaacag ctaaaggcag 660 aagtagaaaa atacaaagac tgtgatccgc aagttgtgga agaaatacgc caagcaaata 720 aagtagccaa aacagctgct aacagatgga ctgataacat attcgcaata aaatcttggg 780 ccaaaagaaa atttgggttt gaagaaaata aaattgatag aacttttgga attccagaag 840 actttgacta catagactaa aatattccat ggtggtgaag gatgtacaag cttgtgaata 900 tgtaaatttt aaactattat ctaactaagt gtactgaatt gtcgtttgcc tgtaactgtg 960 tttatcattt tattaatgtt aaataaagtg taaaatgcaa aaaaaaaaaa aaaaaaaaaa 1020 aaaaaaaa 1028

[0837] TABLE XXIIIA Nucleotide sequence alignment of 121P1F1 with splice variant 1. Score =687 bits (357), Expect = 0.0Identities = 357/357 (100%) Strand = Plus /Plus 121P1F1: 1 ccaaaatcaaacgcgtccgggcctgtcccycccctctccccaagcgcgggcccggccagc 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 1 ccaaaatcaaacgcgtccgggcctgtcccgcccctctccccaagcgcgggcccggccagc 60 121P1F1: 61 ggaagcccctgcgcccgcgccatgtcaaagaaaaaaggactgagtgcagaagaaaagaga 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 61 ggaagcccctgcgcccgcgccatgtcaaagaaaaaaggactgagtgcagaagaaaagaga 120 121P1F1: 121 actcgcatgatggaaatattttctgaaacaaaagatgtatttcaattaaaagacttggag 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 121 actcgcatgatggaaatattttctgaaacaaaagatgtatttcaattaaaagacttggag 180 121P1F1: 181 aagattgctcccaaagagaaaggcattactgctatgtcagtaaaagaagtccttcaaagc 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 181 aagattgctcccaaagagaaaggcattactgctatgtcagtaaaagaagtccttcaaagc 240 121P1F1: 241 ttagttgatgatggtatggttgactgtgagaggatcggaacttctaattattattgggct 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 241 ttagttgatgatggtatggttgactgtgagaggatcggaacttctaattattattgggct 300 121P1F1: 301 tttccaagtaaagctcttcatgcaaggaaacataagttggaggttctggaatctcag 357 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 301 tttccaagtaaagctcttcatgcaaggaaacataagttggaggttctggaatctcag 357 Score =985 bits (512), Expect =0.0Identities =512/512 (100%) Strand =Plus /Plus 121P1F1: 356 agttgtctgagggaagtcaaaagcatgcaagcctacagaaaagcattgagaaagctaaaa 415 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 517 agttgtctgagggaagtcaaaagcatgcaagcctacagaaaagcattgagaaagctaaaa 576 121P1F1: 416 ttggccgatgtgaaacggaagagcgaaccaggctagcaaaagagctttcttcacttcgag 475 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 577 ttggccgatgtgaaacggaagagcgaaccaggctagcaaaagagctttcttcacttcgag 636 121P1F1: 476 accaaagggaacagctaaaggcagaagtagaaaaatacaaagactgtgatccgcaagttg 535 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 637 accaaagggaacagctaaaggcagaagtagaaaaatacaaagactgtgatccgcaagttg 696 121P1F1: 536 tggaagaaatacgccaagcaaataaagtagccaaagaagctgctaacagatggactgata 595 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 697 tggaagaaatacgccaagcaaataaagtagccaaagaagctgctaacagatggactgata 756 121P1F1: 596 acatattcgcaataaaatcttgggccaaaagaaaatttgggtttgaagaaaataaaattg 655 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 757 acatattcgcaataaaatcttgggccaaaagaaaatttgggtttgaagaaaataaaattg 816 121P1F1: 656 atagaacttttggaattccagaagactttgactacatagactaaaatattccatggtggt 715 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 817 atagaacttttggaattccagaagactttgactacatagactaaaatattccatggtggt 876 121P1F1: 716 gaaggatgtacaagcttgtgaatatgtaaattttaaactattatctaactaagtgtactg 775 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 877 gaaggatgtacaagcttgtgaatatgtaaattttaaactattatctaactaagtgtactg 936 121P1F1: 776 aattgtcgtttgcctgtaactgtgtttatcattttattaatgttaaataaagtgtaaaat 835 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 1: 937 aattgtcgtttgcctgtaactgtgtttatcattttattaatgttaaataaagtgtaaaat 996 121P1F1 836 gcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 867 |||||||||||||||||||||||||||||||| Variant 1: 997 gcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1028

[0838] TABLE XXIVA Amino acid sequence alignment of 121P1F1 and splice variant 1. Score =183 bits (465), Expect =6e-47Identities =92/92 (100%) , Positives =92/92 (100%) 121P1F1: 1 MSKKKGLSAEEKRTRNMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV 60 MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV Variant 1A: 1 MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV 60 121P1F1: 61 DCERIGTSNYYWAFPSKALHARKHKLEVLESQ 92 DCERIGTSNYYWAFPSKALHARKHKLEVLESQ Variant 1A: 61 DCERIGTSNYYWAFPSKALHARKHKLEVLESQ 92 Score =229 bits (584), Expect =1e-60Identities =113/114 (99%) , Positives = 114/114 (99%) 121P1F1: 92 QLSEGSQKHASLQKSIEKAKIGRCETEERTRLAKELSSLRDQREQLKAEVEKYKDCDPQV 151 +LSEGSQKHASLQKSIEKAKIGRCETEERTRLAKELSSLRDQREQLKAEVEKYKDCDPQV Variant 1B: 6 ELSEGSQKHASLQKSIEKAKIGRCETEERTRLAKELSSLRDQREQLKAEVEKYKDCDPQV 65 121P1F1: 152 VEEIRQANKVAKEAANRWTDNIFAIKSWAKRKFGFEENKIDRTFGIPEDFDYID 205 VEEIRQANKVAKEAANRWTDNIFAIKSWAKRKFGFEENKIDRTFGIPEDEDYID Variant 1B: 66 VEEIRQANKVAKEAANRWTDNIFAIKSWAKRKFGFEENKIDRTFGIPEDFDYID 119

[0839] TABLE XXVA Peptide sequences from the translation of the nucleotide sequence of splice variant 1. >splice variant 1A ORF:82 . . . 462 Frame +1 MSKKKGLSAE EKRTRMMEIF SETKDVFQLK DLEKIAPKEK GITAMSVKEV LQSLVDDGMV 60 DCERIGTSNY YWAFPSKALH ARKHKLEVLE SQDPGCCFHE IIKVSYYRKF WLGAVAHACN 120 PSTLGG 126 >splice variant 1B ORF:501 . . . 860 Frame +3 MKCKMELSEG SQKHASLQKS IEKAKIGRCE TEERTRLAKE LSSLRDQREQ LKAEVEKYIT 60 CDPQVVEEIR QANKVAKEAA NRWTDNIFAI KSWAKRKFGF EENKIDRTFG IPEDFDYID 119

[0840] TABLE XXIIB Nucleotide sequence of splice variant 2. ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cggccaagca aataaagtag ccaaagaagc tgctaacaga tggactgata 480 acatattcgc aataaaatct tgggccaaaa gaaaatttgg gtttgaagaa aataaaattg 540 atagaacttt tggaattcca gaagactttg actacataga ctaaaatatt ccatggtggt 600 gaaggatgta caagcttgtg aatatgtaaa ttttaaacta ttatctaact aagtgtactg 660 aattgtcgtt tgcctgtaac tgtgtttatc attttattaa tgttaaataa agtgtaaaat 720 gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 752

[0841] TABLE XXIIIB Nucleotide sequence alignment of 121P1F1 with splice variant 2. Score =833 bits (433), Expect =0.0Identities =433/433 (100%) Strand Plus /Plus 121P1F1: 1 ccaaaatcaaacgcgtccgggcctgtcccgcccctctccccaagcgcgggcccggccagc 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 1 ccaaaatcaaacgcgtccgggcctgtcccgcccctctccccaagcgcgggcccggccagc 60 121P1F1: 61 ggaagcccctgcgcccgcgccatgtcaaagaaaaaaggactgagtgcagaagaaaagaga 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 61 ggaagcccctgcgcccgcgccatgtcaaagaaaaaaggactgagtgcagaagaaaagaga 120 121P1F1: 121 actcgcatgatggaaatattttctgaaacaaaagatgtatttcaattaaaagacttggag 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 121 actcgcatgatggaaatattttctgaaacaaaagatgtatttcaattaaaagacttggag 180 121P1F1: 181 aagattgctcccaaagagaaaggcattactgctatgtcagtaaaagaagtccttcaaagc 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 181 aagattgctcccaaagagaaaggcattactgctatgtcagtaaaagaagtccttcaaagc 240 121P1F1: 241 ttagttgatgatggtatggttgactgtgagaggatcggaacttctaattattattgggct 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 241 ttagttgatgatggtatggttgactgtgagaggatcggaacttctaattattattgggct 300 121P1F1: 301 tttccaagtaaagctcttcatgcaaggaaacataagttggaggttctggaatctcagttg 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 301 tttccaagtaaagctcttcatgcaaggaaacataagttggaggttctggaatctcagttg 360 121P1F1: 361 tctgagggaagtcaaaagcatgcaagcctacagaaaagcattgagaaagctaaaattggc 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 361 tctgagggaagtcaaaagcatgcaagcctacagaaaagcattgagaaagctaaaattggc 420 121P1F1: 421 cgatgtgaaacgg 433 ||||||||||||| Variant 2: 421 cgatgtgaaacgg 433 Score =615 bits (320) Expect =e-173Identities =320/320 (100%) Strand =Plus /Plus 121P1F1: 548 gccaagcaaataaagtagccaaagaagctgctaacagatggactgataacatattcgcaa 607 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 433 gccaagcaaataaagtagccaaagaagctgctaacagatggactgataacatattcgcaa 492 121P1F1: 608 taaaatcttgggccaaaagaaaatttgggtttgaagaaaataaaattgatagaacttttg 667 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 493 taaaatcttgggccaaaagaaaatttgggtttgaagaaaataaaattgatagaacttttg 552 121P1F1: 668 gaattccagaagactttgactacatagactaaaatattccatggtggtgaaggatgtaca 727 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 553 gaattccagaagactttgactacatagactaaaatattccatggtggtgaaggatgtaca 612 121P1F1: 728 agcttgtgaatatgtaaattttaaactattatctaactaagtgtactgaattgtcgtttg 787 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 613 agcttgtgaatatgtaaattttaaactattatctaactaagtgtactgaattgtcgtttg 672 121P1F1: 788 cctgtaactgtgtttatcattttattaatgttaaataaagtgtaaaatgcaaaaaaaaaa 847 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 2: 673 cctgtaactgtgtttatcatattattaatgttaaataaagtgtaaaatgcaaaaaaaaaa 732 121P1F1: 648 aaaaaaaaaaaaaaaaaaaa 867 |||||||||||||||||||| Variant 2: 733 aaaaaaaaaaaaaaaaaaaa 752

[0842] TABLE XXIVB Amino acid sequence alignment of 121P1F1 and splice variant 2. Score =232: bits (591), Expect =2e-61Identities =117/122 (95%) , Positives = 120/122 (97%) 121P1F1: 1 MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV 60 MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV Variant 2: 2 MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV 60 121P1F1: 61 DCERIGTSNYYWAFPSKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEER 120 DCERIGTSNYYWAFPSKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCET ++ Variant 2: 61 DCERIGTSNYYWAFPSKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETAKQ 120 121P1F1: 121 TR 122 + Variant 2: 121 IK 122

[0843] TABLE XXIIC Nucleotide sequence of splice variant 3. ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cggaagagcg aaccaggcta gcaaaagagc tttcttcact tcgagaccaa 480 agggaacagc taaaggcaga agtagaaaaa tacaaagact gtgatccgca agttgtggaa 540 gaaatacata acatattcgc aataaaatct tgggccaaaa gaaaatttgg gtttgaagaa 600 aataaaattg atagaacttt tggaattcca gaagactttg actacataga ctaaaatatt 660 ccatggtggt gaaggatgta caagcttgtg aatatgtaaa ttttaaacta ttatctaact 720 aagtgtactg aattgtcgtt tgcctgtaac tgtgtttatc attttattaa tgttaaataa 780 agtgtaaaat gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 822

[0844] TABLE XXVB Peptide sequences from the translation of the nucleotide sequence of splice variant 2. MSKKKGLSAE EKRTRMMEIF SETKDVFQLK DLEKIAPKEK GITAMSVKEV LQSLVDDGNV 60 DCERIGTSNY YWAFPSKALH ARKHKLEVLE SQLSEGSQKH ASLQKSIEKA KIGRCETAKQ 120 IK

[0845] TABLE XXIIIC Nucleotide sequence alignment of 121P1F1 with splice variant 3. Score =1052 bits (547), Expect =0.0Identities =547/547 (100%) Strand =Plus /Plus 121P1F1: 1 ccaaaatcaaacgcgtccgggcctgtcccgcccctctccccaagcgcgggcccggccagc 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 1 ccaaaatcaaacgcgtccgggcctgtcccgcccctctccccaagcgcgggcccggccagc 60 121P1F1: 61 ggaagcccctgcgcccgcgccatgtcaaagaaaaaaggactgagtgcagaagaaaagaga 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 61 ggaagcccctgcgcccgcgccatgtcaaagaaaaaaggactgagtgcagaagaaaagaga 120 121P1F1: 121 actcgcatgatggaaatattttctgaaacaaaagatgtatttcaattaaaagacttggag 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 121 actcgcatgatggaaatattttctgaaacaaaagatgtatttcaattaaaagacttggag 180 121P1F1: 181 aagattgctcccaaagagaaaggcattactgctatgtcagtaaaagaagtccttcaaagc 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 181 aagattgctcccaaagagaaaggcattactgctatgtcagtaaaagaagtccttcaaagc 240 121P1F1: 241 ttagttgatgatggtatggttgactgtgagaggatcggaacttctaattattattgggct 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 241 ttagttgatgatggtatggttgactgtgagaggatcggaacttctaattattattgggct 300 121P1F1: 301 tttccaagtaaagctcttcatgcaaggaaacataagttggaggttctggaatctcagttg 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 301 tttccaagtaaagctcttcatgcaaggaaacataagttggaggttctggaatctcagttg 360 121P1F1: 361 tctgagggaagtcaaaagcatgcaagcctacagaaaagcattgagaaagctaaaattggc 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 361 tctgagggaagtcaaaagcatgcaagcctacagaaaagcattgagaaagctaaaattggc 420 121P1F1: 421 cgatgtgaaacggaagagcgaaccaggctagcaaaagagctttcttcacttcgagaccaa 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 421 cgatgtgaaacggaagagcgaaccaggctagcaaaagagctttcttcacttcgagaccaa 480 121P1F1: 481 agggaacagctaaaggcagaagtagaaaaatacaaagactgtgatccgcaagttgtggaa 540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 481 agggaacayctaaaggcagaagtagaaaaatacaaagactgtgatccgcaagttgtggaa 540 121P1F1: 541 gaaatac 547 ||||||| Variant 3: 541 gaaatac 547 Score =529 bits (275), Expect =e-147Identities =275/275 (100%) Strand =Plus /Plus 121P1F1: 593 ataacatattcgcaataaaatcttgggccaaaagaaaatttgggtttgaagaaaataaaa 652 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 548 ataacatattcgcaataaaatcttgggccaaaagaaaatttgggtttgaagaaaataaaa 607 121P1F1: 653 ttgatagaacttttggaattccagaagactttgactacatagactaaaatattccatggt 712 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 608 ttgatagaacttttggaattccagaagactttgactacatagactaaaatattccatggt 667 121P1F1: 713 ggtgaaggatgtacaagcttgtgaatatgtaaattttaaactattatctaactaagtgta 772 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 668 ggtgaaggatgtacaagcttgtgaatatgtaaattttaaactattatctaactaagtgta 727 121P1F1: 773 ctgaattgtcgtttgcctgtaactgtgtttatcattttattaatgttaaataaagtgtaa 832 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 3: 728 ctgaattgtcgtttgcctgtaactgtgtttatcattttattaatgttaaataaagtgtaa 787 121P1F1: 833 aatgcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 867 ||||||||||||||||||||||||||||||||||| Variant 3: 788 aatgcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 822

[0846] TABLE XXIVC Amino acid sequence alignment of 121P1F1 and splice variant 3. Score = 365 bits (937), Expect = e−101Identities = 189/205 (92%), Positives = 189/205 (92%) , Gaps = 15/205 (7%) 121P1F12: 1 MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV 60 MSKKKGLSAEEKRTRMMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV Variant 3: 1 MSKKKGLSAEEKRTRMMEIFSETKTVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMV 60 121P1F1: 61 DCERIGTSNYYWAFPSKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEER 120 DCERIGTSNYYWAFPSKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEER Variant 3: 61 DCERIGTSNYYWAFPSKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEER 120 121P1F1: 121 TRLAKELSSLRDQREQLKAEVEKYKDCDPQVVEEIRQANKVAKEAANRWTDNIFAIKSWA 180 TRLAKELSSLRDQREQLKAEVEKYKDCDPQVVEEI                NIFAIKSWA Variant 3: 121 TRLAKELSSLRDQREQLKAEVEKYKDCDPQVVEEIH---------------NIFAIKSWA 165 121P1F1: 181 KRKFGFEENKIDRTFGIPEDFDYID 205 KRKFGFEENKIDRTFGIPEDFDYID Variant 3: 166 KRKFGFEENKIDRTFGIPEDFDYID 190

[0847] TABLE XXVC Peptide sequences from the translation of the nucleotide sequence of splice variant 3. MSKKKGLSAE EKRTRMMEIF SETKDVFQLK DLEKIAPKEK GITAMSVKEV LQSLVDDGMV 60 DCERIGTSNY YWAFPSKALH ARKHKLEVLE SQLSEGSQKH ASLQKSIEKA KIGRCETEER 120 TRLAKELSSL RDQREQLKAE VEKYKDCDPQ VVEEIHNIFA IKSWAKRKFG FEENKIDRTF 180 GIPEDFDYID 190

[0848] TABLE XXIID Nucleotide sequence of splice variant 4. gttttctgta ttgtaatatg tagagcacat tccagaactg ctcagtttcg agttacctaa 60 tggatcttca ctgtgtgcca attagtcgat ttctgtgaaa acgccccggt ttctgccaaa 120 gggcaggagt cgctgctctt gtgccgggtg ctgctggttg tgtagggcgc tgttgctttt 180 ttaaggacgc tctgcactga attaggcttc ctcgtgggtc atgatcagtt aagtcctgtc 240 aaagaaaaaa ggactgagtg cagaagaaaa gagaactcgc atgatggaaa tattttctga 300 aacaaaagat gtatttcaat taaaagactt ggagaagatt gctcccaaag agaaaggcat 360 tactgctatg tcagtaaaag aagtccttca aagcttagtt gatgatggta tggttgactg 420 tgagaggatc ggaacttcta attattattg ggcttttcca agtaaagctc ttcatgcaag 480 gaaacataag ttggaggttc tggaatctca gttgtctgag ggaagtcaaa agcatgcaag 540 cctacagaaa agcattgaga aagctaaaat tggccgatgt gaaacggaag agcgaaccag 600 gctagcaaaa gagctttctt cacttcgaga ccaaagggaa cagctaaagg cagaagtaga 660 aaaatacaaa gactgtgatc cgcaagttgt ggaagaaata cgccaagcaa ataaagtagc 720 caaagaagct gctaacagat ggactgataa catattcgca ataaaatctt gggccaaaag 780 aaaatttggg tttgaagaaa ataaaattga tagaactttt ggaattccag aagactttga 840 ctacatagac taaaatattc catggtggtg aaggatgtac aagcttgtga atatgtaaat 900 tttaaactat tatctaacta agtgtactga attgtcgttt gcctgtaact gtgtttatca 960 ttttattaat gttaaataaa gtgtaaaatg cagatgttct tcaccccttt tggtagaaca 1020 aaagcaggat gataaccata tccccccagt gctcatcaaa gtaggacact aaaaatccat 1080 ccatctcagt caaagtcgag cggccgcgaa tttagtagta gtagcggccg ctctagagga 1140 tccaagctta cgtacgcgtg catgcgacgt catagctctt ctatagtgtc acctaaattc 1200 aagtt 1205

[0849] TABLE XXIIID Nucleotide sequence alignment of 121P1F1 with splice variant 4. Score = 1454 bits (756), Expect = 0.0Identities = 756/756 (100%) Strand = Plus/Plus 121P1F1: 83 tgtcaaagaaaaaaggactgagtgcagaagaaaagagaactcgcatgatggaaatatttt 142 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 237 tgtcaaagaaaaaaggactgagtgcagaagaaaagagaactcgcatgatggaaatatttt 296 121P1F1: 143 ctgaaacaaaagatgtatttcaattaaaagacttggagaagattgctcccaaagagaaag 202 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 297 ctgaaacaaaagatgtatttcaattaaaagacttggagaagattgctcccaaagagaaag 356 121P1F1: 203 gcattactgctatgtcagtaaaagaagtccttcaaagcttagttgatgatggtatggttg 262 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 257 gcattactgctatgtcagtaaaagaagtccttcaaagcttagttgatgatggtatggttg 416 121P1F1: 263 actgtgagaggatcggaacttctaattattattgggcttttccaagtaaagctcttcatg 322 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 417 actgtgagaggatcggaacttctaattattattgggcttttccaagtaaagctcttcatg 476 121P1F1: 323 caaggaaacataagttggaggttctggaatctcagttgtctgagggaagtcaaaagcatg 382 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 477 caaggaaacataagttggaggttctggaatctcagttgtctgagggaagtcaaaagcatg 536 121P1F1: 83 caagcctacagaaaagcattgagaaagctaaaattggccgatgtgaaacggaagagcgaa 442 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 537 caagcctacagaaaagcattgagaaagctaaaattggccgatgtgaaacggaagagcgaa 596 121P1F1: 443 ccaggctagcaaaagagctttcttcacttcgagaccaaagggaacagctaaaggcagaag 502 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 597 ccaggctagcaaaagagctttcttcacttcgagaccaaagggaacagctaaaggcagaag 656 121P1F1: 503 tagaaaaatacaaagactgtgatccgcaagttgtggaagaaatacgccaagcaaataaag 562 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 657 tagaaaaatacaaagactgtgatccgcaagttgtggaagaaatacgccaagcaaataaag 716 121P1F1: 563 tagccaaagaagctgctaacagatggactgataacatattcgcaataaaatcttgggcca 622 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 717 tagccaaagaagctgctaacagatggactgataacatattcgcaataaaatcttgggcca 776 121P1F1: 623 aaagaaaatttgggtttgaagaaaataaaattgatagaacttttggaattccagaagact 682 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 777 aaagaaaatttgggtttgaagaaaataaaattgatagaacttttggaattccagaagact 836 121P1F1: 683 ttgactacatagactaaaatattccatggtggtgaaggatgtacaagcttgtgaatatgt 742 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 837 ttgactacatagactaaaatattccatggtggtgaaggatgtacaagcttgtgaatatgt 896 121P1F1: 743 aaattttaaactattatctaactaagtgtactgaattgtcgtttgcctgtaactgtgttt 802 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Variant 4: 897 aaattttaaactattatctaactaagtgtactgaattgtcgtttgcctgtaactgtgttt 956 121P1F1: 803 atcattttattaatgttaaataaagtgtaaaatgca 838 |||||||||||||||||||||||||||||||||||| Variant 4: 957 atcattttattaatgttaaataaagtgtaaaatgca 992

[0850] TABLE XXIVD Amino acid sequence alignment of 121P1F1 and splice variant 4. Score = 380 bits (975), Expect = e-105Identities = 190/190 (100%), Positives = 190/190 (100%) 121P1F1: 16 MMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMVDCERIGTSNYYWAFP 75 MMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMVDCERIGTSNYYWAFP Variant 4: 1 MMEIFSETKDVFQLKDLEKIAPKEKGITAMSVKEVLQSLVDDGMVDCERIGTSNYYWAFP 60 121P1F1: 76 SKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEERTRLAKELSSLRDQRE 135 SKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEERTRLAKELSSLRDQRE Variant 4: 61 SKALHARKHKLEVLESQLSEGSQKHASLQKSIEKAKIGRCETEERTRLAKELSSLRDQRE 120 121P1F1: 136 QLKAEVEKYKDCDPQVVEEIRQANKVAKEAANRWTDNIFAIKSWAKRKFGFEENKIDRTF 195 QLKAEVEKYKDCDPQVVEEIRQANKVAKEAANRWTDNIFAIKSWAKRKFGFEENKIDRTF Variant 4: 121 QLKAEVEKYKDCDPQVVEEIRQANKVAKEAANRWTDNIFAIKSWAKRKFGFEENKIDRTF 180 12P1F1: 196 GIPEDFDYID 205 GIPEDFDYID Variant 4: 181 GIPEDFDYID 190

[0851] TABLE XXVD Peptide sequences from the translation of the nucleotide sequence of splice variant 4. MMEIFSETKD VFQLKDLEKI APKEKGITAM SVKEVLQSLV DDGMVDCERI GTSNYYWAFP 60 SKALHARKHK LEVLESQLSE GSQKHASLQK SIEKAKIGRC ETEERTRLAK ELSSLRDQRE 120 QLKAEVEKYK DCDPQVVEEI RQANKVAKEA ANRWTDNIFA IKSWAKRKFG FEENKIDRTF 130 GIPEDFDYID 190

[0852] TABLE XXVI MHC Class 1 nonamer and decamer analysis of 121P1F1 for selected alleles. Listed are scores that fall within the top 50% (rounded up) of all scores for the selected allele. Pos 123456789 score SEQ ID NO: HLA-A*0201 nonamers 122 RLAKELSSL 28 78 ALHARKHKL 25 42 ITAMSVKEV 23 46 SVKEVLQSL 23 129 SLRDQREQL 23 34 KIAPKEKGI 22 102 SLQKSIEKA 22 85 KLEVLESQL 21 196 GIPEDFDYI 19 15 RMMEIFSET 17 18 EIFSETKDV 17 27 FQLKDLEKI 17 80 HARKHKLEV 17 165 AANRWTDNI 17 50 VLQSLVDDG 16 81 ARKHKLEVL 16 88 VLESQLSEG 16 92 QLSEGSQKH 16 21 SETKDVFQL 15 43 TAMSVKEVL 15 136 QLKAEVEKY 15 6 GLSAEEKRT 14 28 QLKDLEKIA 14 71 YWAFPSKA L 14 133 QREQLKAEV 14 147 CDPQVVEEI 14 150 QVVEEIRQA 14 189 NKIDRTFGI 14 HLA-A1 nonamers 195 FG IPEDFDY 20 136 QL KAEVEKY 19 169 WT DNIFAIK 19 23 TK DVFQLKD 18 116 ET EERTRLA 18 62 CE RIGTSNY 17 117 TE ERTRLAK 17 124 AK ELSSLRD 17 146 DC DPQVVEE 17 63 ER IGTSNYY 16 106 SI EKAKIGR 16 20 FS ETKDVFQ 15 59 MV DCERIGT 15 93 LS EGSQKHA 15 29 LK DLEKIAP 14 88 VL ESQLSEG 14 185 GF EENKIDR 14 8 SA EEKRTRM 13 22 ET KDVFQLK 13 31 DL EKIAPKE 13 47 VK EVLQSLV 13 55 VD DGMVDCE 13 144 YK DCDPQVV 13 190 KI DRTFGIP 13 9 AE EKRTRMM 12 37 PK EKGITAM 12 54 LV DDGMVDC 12 130 LR DQREQLK 12 138 KA EVEKYKD 12 151 VV EEIRQAN 12 162 AK EAANRWT 12 1 MS KKKGLSA 11 45 MS VKEVLQS 11 61 DC ERIGTSN 11 85 KL EVLESQL 11 140 EV EKYKDCD 11 152 VE EIRQANK 11 186 FE ENKIDRT 11 13 RT RMMEIFS 10 16 MM EIFSETK 10 114 RC ETEERTR 10 133 QR EQLKAEV 10 197 IP EDFDYID 10 HLA-A26 nonamers 46 SVKEVLQSL 27 66 GTSNYYWAF 25 122 RLAKELSSL 24 136 QLKAEVEKY 24 193 RTFGIPEDF 24 22 ETKDVFQLK 23 49 EVLQSLVDD 23 25 DVFQLKDLE 20 63 ERIGTSNYY 20 87 EVLESQLSE 20 18 EIFSETKDV 19 85 KLEVLESQL 19 129 SLRDQREQL 19 19 IFSETKDVF 18 95 EGSQKHASL 18 116 ETEERTRLA 18 31 DLEKIAPKE 17 42 ITAMSVKEV 17 54 LVDDGMVDC 17 78 ALHARKHKL 17 126 ELSSLRDQR 17 140 EVEKYKDCD 17 150 QVVEEIRQA 17 154 EIRQANKVA 17 187 EENKIDRTF 17 196 GIPEDFDYI 17 88 VLESQLSEG 16 119 ERTRLAKEL 16 146 DCDPQVVEE 16 169 WTDNIFAIK 16 34 KIAPKEKGI 15 102 SLQKSIEKA 15 190 KIDRTFGIP 15 12 KRTRMMEIF 14 21 SETKDVFQL 14 37 PKEKGITAM 14 50 VLQSLVDDG 14 81 ARKHKLEVL 14 132 DQREQLKAE 14 151 VVEEIRQAN 14 160 KVAKEAANR 14 195 FGIPEDFDY 14 24 KDVFQLKDL 13 171 DNIFAIKSW 13 172 NIFAIKSWA 13 175 AIKSWAKRK 13 178 SWAKRKFGF 13 HLA-A3 nonamers 175 AI KSWA KRK 25 160 KV AKEA ANR 24 40 KG ITAM SVK 23 91 SQ LSEG SQK 22 136 QL KAEV EKY 21 30 KD LEKI APK 20 53 SL VDDG MVD 20 122 RL AKEL SSL 20 85 KL EVLE SQL 19 92 QL SEGS QKH 19 129 SL RDQR EQL 19 155 IR QANK VAK 19 87 EV LESQ LSE 18 97 SQ KHAS LQK 18 117 TE ERTR LAK 18 126 EL SSLR DQR 18 4 KK GLSA EEK 17 54 LV DDGM VDC 17 78 AL HARK HKL 17 34 KI APKE KGI 16 46 SV KEVL QSL 16 49 EV LQSL VDD 16 69 NY YWAF PSK 16 75 PS KALH ARK 16 77 KA LHAR KHK 16 101 AS LQKS IEK 16 135 EQ LKAE VEK 16 150 QV VEEI RQA 16 152 VE EIRQ ANK 16 173 IF AIKS WAK 16 182 RK FGFE ENK 16 16 MM EIFS ETK 15 26 VF QLKD LEK 15 62 CE RIGT SNY 15 111 KI GRCE TEE 15 154 EI RQAN KVA 15 190 KI DRTF GIP 15 28 QL KDLE KIA 14 41 GI TAMS VKE 14 110 AK IGRC ETE 14 169 WT DNIF AIK 14 172 NI FAIK SWA 14 22 ET KDVF QLK 13 31 DL EKIA PKE 13 32 LE KIAP KEK 13 36 AP KEKG ITA 13 88 VL ESQL SEG 13 106 SI EKAK IGR 13 134 RE QLKA EVE 13 137 LK AEVE KYK 13 151 VV EEIR QAN 13 6 GL SAEE KRT 12 64 RI GTSN YYW 12 103 LQ KSIE KAK 12 114 RC ETEE RTR 12 130 LR DQRE QLK 12 145 KD CDPQ VVE 12 195 FG IPED FDY 12 HLA-B*0702 nonamers 36 APKEKGITA 19 71 YWAFPSKAL 15 74 FPSKALHAR 14 95 EGSQKHASL 14 78 ALHARKHKL 13 81 ARKHKLEVL 13 122 RLAKELSSL 13 129 SLRDQREQL 13 21 SETKDVFQL 12 43 TAMSVKEVL 12 115 CETEERTRL 12 24 KDVFQLKDL 11 80 HARKHKLEV 11 85 KLEVLESQL 11 119 ERTRLAKEL 11 197 IPEDFDYID 11 1 MSKKKGLSA 10 9 AEEKRTRMM 10 19 IFSETKDVF 10 46 SVKEVLQSL 10 73 AFPSKALHA 10 148 DPQVVEEIR 10 154 EIRQANKVA 10 166 ANRWTDNIF 10 6 GLSAEEKRT 9 11 EKRTRMMEI 9 15 RMMEIFSET 9 34 KIAPKEKGI 9 37 PKEKGITAM 9 42 ITAMSVKEV 9 66 GTSNYYWAF 9 104 QKSIEKAKI 9 131 RDQREQLKA 9 158 ANKVAKEAA 9 162 AKEAANRWT 9 165 AANRWTDNI 9 176 IKSWAKRKF 9 193 RTFGIPEDF 9 HLA-B*08 nonamers 81 ARKHKLEVL 30 36 APKEKGITA 28 46 SVKEVLQSL 24 78 ALHARKHKL 24 129 SLRDQREQL 24 179 WAKRKFGFE 24 11 EKRTRMMEI 23 95 EGSQKHASL 22 107 IEKAKIGRC 22 141 VEKYKDCDP 22 34 KIAPKEKGI 21 1 MSKKKGLSA 20 8 SAEEKRTRM 18 28 QLKDLEKIA 17 85 KLEVLESQL 17 136 QLKAEVEKY 17 161 VAKEAANRW 17 118 EERTRLAKE 16 122 RLAKELSSL 16 123 LAKELSSLR 16 178 SWAKRKFGF 16 109 KAKIGRCET 15 175 AIKSWAKRK 15 HLA-B*1510 nonamers 43 TAMSVKEVL 14 71 YWAFPSKAL 14 115 CETEERTRL 14 19 IFSETKDVF 13 95 EGSQKHASL 13 21 SETKDVFQL 12 81 ARKHKLEVL 12 83 KHKLEVLES 12 85 KLEVLESQL 12 119 ERTRLAKEL 12 122 RLAKELSSL 12 129 SLRDQREQL 12 176 IKSWAKRKF 12 8 SAEEKRTRM 11 37 PKEKGITAM 11 46 SVKEVLQSL 11 78 ALHARKHKL 11 79 LHARKHKLE 11 99 KHASLQKSI 11 187 EENKIDRTF 11 9 AEEKRTRMM 10 24 KDVFQLKDL 10 66 GTSNYYWAF 9 178 SWAKRKFGF 9 193 RTFGIPEDF 8 12 KRTRMMEIF 7 51 LQSLVDDGM 7 155 IRQANKVAK 7 HLA-B*2705 nonamers 63 ERIGTSNYY 24 81 ARKHKLEVL 24 119 ERTRLAKEL 23 155 IRQANKVAK 23 12 KRTRMMEIF 22 130 LRDQREQLK 22 182 RKFGFEENK 21 30 KDLEKIAPK 20 122 RLAKELSSL 20 193 RTFGIPEDF 20 101 ASLQKSIEK 19 160 KVAKEAANR 19 174 FAIKSWAKR 18 37 PKEKGITAM 17 192 DRTFGIPED 17 4 KKGLSAEEK 16 5 KGLSAEEKR 16 40 KGITAMSVK 16 113 GRCETEERT 16 114 RCETEERTR 16 115 CETEERTRL 16 133 QREQLKAEV 16 135 EQLKAEVEK 16 185 GFEENKIDR 16 14 TRMMEIFSE 15 26 VFQLKDLEK 15 72 WAFPSKALH 15 85 KLEVLESQL 15 91 SQLSEGSQK 15 95 EGSQKHASL 15 121 TRLAKELSS 15 152 VEEIRQANK 15 181 KRKFGFEEN 15 187 EENKIDRTF 15 7 LSAEEKRTR 14 8 SAEEKRTRM 14 19 IFSETKDVF 14 21 SETKDVFQL 14 24 KDVFQLKDL 14 46 SVKEVLQSL 14 66 GTSNYYWAF 14 69 NYYWAFPSK 14 75 PSKALHARK 14 77 KALHARKHK 14 78 ALHARKHKL 14 92 QLSEGSQKH 14 106 SIEKAKIGR 14 123 LAKELSSLR 14 173 IFAIKSWAK 14 175 AIKSWAKRK 14 176 IKSWAKRKF 14 27 FQLKDLEKI 13 43 TAMSVKEVL 13 56 DDGMVDCER 13 62 CERIGTSNY 13 74 FPSKALHAR 13 97 SQKHASLQK 13 112 IGRCETEER 13 166 ANRWTDNIF 13 168 RWTDNIFAI 13 178 SWAKRKFGF 13 195 FGIPEDFDY 13 16 MMEIFSETK 12 71 YWAFPSKAL 12 76 SKALHARKH 12 99 KHASLQKSI 12 126 ELSSLRDQR 12 136 QLKAEVEKY 12 137 LKAEVEKYK 12 167 NRWTDNIFA 12 169 WTDNIFAIK 12 183 KFGFEENKI 12 HLA-B*2709 nonamers 119 ERTRLAKEL 22 12 KRTRMMEIF 21 81 ARKHKLEVL 21 133 QREQLKAEV 18 193 RTFGIPEDF 15 21 SETKDVFQL 14 113 GRCETEERT 14 122 RLAKELSSL 14 24 KDVFQLKDL 13 85 KLEVLESQL 13 121 TRLAKELSS 13 168 RWTDNIFAI 13 115 CETEERTRL 12 143 KYKDCDPQV 12 155 IRQANKVAK 12 181 KRKFGFEEN 12 192 DRTFGIPED 12 196 GIPEDFDYI 12 18 EIFSETKDV 11 27 FQLKDLEKI 11 34 KIAPKEKGI 11 43 TAMSVKEVL 11 52 QSLVDDGMV 11 63 ERIGTSNYY 11 66 GTSNYYWAF 11 78 ALHARKHKL 11 99 KHASLQKSI 11 129 SLRDQREQL 11 167 NRWTDNIFA 11 HLA-B*4402 nonamers 187 EENKIDRTF 25 21 SETKDVFQL 23 62 CERIGTSNY 21 115 CETEERTRL 21 153 EEIRQANKV 19 171 DNIFAIKSW 18 63 ERIGTSNYY 17 9 AEEKRTRMM 16 78 ALHARKHKL 16 118 EERTRLAKE 16 119 ERTRLAKEL 16 195 FGIPEDFDY 16 81 ARKHKLEVL 15 117 TEERTRLAK 15 139 AEVEKYKDC 15 168 RWTDNIFAI 15 189 NKIDRTFGI 15 193 RTFGIPEDF 15 10 EEKRTRMME 14 17 MEIFSETKD 14 24 KDVFQLKDL 14 34 KIAPKEKGI 14 38 KEKGITAMS 14 48 KEVLQSLVD 14 66 GTSNYYWAF 14 71 YWAFPSKAL 14 94 SEGSQKHAS 14 125 KELSSLRDQ 14 129 SLRDQREQL 14 163 KEAANRWTD 14 166 ANRWTDNIF 14 186 FEENKIDRT 14 32 LEKIAPKEK 13 95 EGSQKHASL 13 107 IEKAKIGRC 13 134 REQLKAEVE 13 165 AANRWTDNI 13 176 IKSWAKRKF 13 11 EKRTRMMEI 12 12 KRTRMMEIF 12 19 IFSETKDVF 12 43 TAMSVKEVL 12 46 SVKEVLQSL 12 85 KLEVLESQL 12 86 LEVLESQLS 12 136 QLKAEVEKY 12 161 VAKEAANRW 12 178 SWAKRKFGF 12 uz,10/23 HLA-B*5101 nonamers 43 TAMSVKEVL 22 57 DGMVDCERI 21 80 HARKHKLEV 20 165 AANRWTDNI 20 27 FQLKDLEKI 17 36 APKEKGITA 16 148 DPQVVEEIR 16 161 VAKEAANRW 16 8 SAEEKRTRM 15 147 CDPQVVEEI 15 157 QANKVAKEA 15 174 FAIKSWAKR 15 35 IAPKEKGIT 14 42 ITAMSVKEV 14 77 KALHARKHK 14 123 LAKELSSLR 14 144 YKDCDPQVV 14 196 GIPEDFDYI 14 74 FPSKALHAR 13 95 EGSQKHASL 13 183 KFGFEENKI 13 197 IPEDFDYID 13 34 KIAPKEKGI 12 72 WAFPSKALH 12 104 QKSIEKAKI 12 138 KAEVEKYKD 12 153 EEIRQANKV 12 168 RWTDNIFAI 12 179 WAKRKFGFE 12 184 FGFEENKID 12 189 NKIDRTFGI 12 11 EKRTRMMEI 11 46 SVKEVLQSL 11 81 ARKHKLEVL 11 99 KHASLQKSI 11 164 EAANRWTDN 11 Pos 1234567890 score SEQ ID NO: HLA-A*0201 decamers 41 GITAMSVKEV 23 77 KALHARKHKL 20 42 ITAMSVKEVL 18 80 HARKHKLEVL 18 121 TRLAKELSSL 18 34 KIAPKEKGIT 17 46 SVKEVLQSLV 17 79 LHARKHKLEV 17 45 MSVKEVLQSL 16 50 VLQSLVDDGM 16 94 SEGSQKHASL 16 26 VFQLKDLEKI 15 44 AMSVKEVLQS 15 53 SLVDDGMVDC 15 58 GMVDCERIGT 15 92 QLSEGSQKHA 15 132 DQREQLKAEV 15 146 DCDPQVVEEI 15 20 FSETKDVFQL 14 38 KEKGITAMSV 14 84 HKLEVLESQL 14 101 ASLQKSIEKA 14 128 SSLRDQREQL 14 167 NRWTDNIFAI 14 182 RKFGFEENKI 14 6 GLSAEEKRTR 13 15 RMMEIFSETK 13 23 TKDVFQLKDL 13 64 RIGTSNYYWA 13 70 YYWAFPSKAL 13 103 LQKSIEKAKI 13 106 SIEKAKIGRC 13 129 SLRDQREQLK 13 152 VEEIRQANKV 13 195 FGIPEDFDYI 13 35 IAPKEKGITA 12 36 APKEKGITAM 12 51 LQSLVDDGMV 12 72 WAFPSKALHA 12 102 SLQKSIEKAK 12 122 RLAKELSSLR 12 196 GIPEDFDYID 12 HLA-A*0203 decamers 157 QA NKVAKEAA 19 158 AN KVAKEAAN 17 27 FQ LKDLEKIA 10 35 IA PKEKGITA 10 64 RI GTSNYYWA 10 69 NY YWAFPSKA 10 72 WA FPSKALHA 10 92 QL SEGSQKHA 10 101 AS LQKSIEKA 10 115 CE TEERTRLA 10 130 LR DQREQLKA 10 149 PQ VVEEIRQA 10 153 EE IRQANKVA 10 156 RQ ANKVAKEA 10 166 AN RWTDNIFA 10 171 DN IFAIKSWA 10 1 MS KKKGLSAE 9 28 QL KDLEKIAP 9 36 AP KEKGITAM 9 65 IG TSNYYWAF 9 70 YY WAFPSKAL 9 73 AF PSKALHAR 9 93 LS EGSQKHAS 9 102 SL QKSIEKAK 9 116 ET EERTRLAK 9 131 RD QREQLKAE 9 150 QV VEEIRQAN 9 154 EI RQANKVAK 9 167 NR WTDNIFAI 9 172 NI FAIKSWAK 9 HLA-A1 decamers 61 DC ERIGTSNY 25 116 ET EERTRLAK 23 169 WT DNIFAIKS 22 47 VK EVLQSLVD 18 130 LR DQREQLKA 18 135 EQ LKAEVEKY 18 20 FS ETKDVFQL 16 62 CE RIGTSNYY 15 93 LS EGSQKHAS 15 146 DC DPQVVEEI 15 190 KI DRTFGIPE 15 194 TF GIPEDFDY 15 22 ET KDVFQLKD 14 8 SA EEKRTRMM 13 9 AE EKRTRMME 13 85 KL EVLESQLS 13 144 YK DCDPQVVE 13 152 VE EIRQANKV 13 16 MM EIFSETKD 12 55 VD DGMVDCER 12 88 VL ESQLSEGS 12 106 SI EKAKIGRC 12 117 TE ERTRLAKE 12 120 RT RLAKELSS 12 162 AK EAANRWTD 12 HLA-A26 decamers 18 EIFSETKDVF 29 87 EVLESQLSEG 24 175 AIKSWAKRKF 23 135 EQLKAEVEKY 22 49 EVLQSLVDDG 21 11 EKRTRMMEIF 20 25 DVFQLKDLEK 20 22 ETKDVFQLKD 19 42 ITAMSVKEVL 19 116 ETEERTRLAK 19 154 EIRQANKVAK 19 50 VLQSLVDDGM 18 61 DCERIGTSNY 18 126 ELSSLRDQRE 17 140 EVEKYKDCDP 17 31 DLEKIAPKEK 16 36 APKEKGITAM 16 54 LVDDGMVDCE 16 65 IGTSNYYWAF 16 106 SIEKAKIGRC 16 192 DRTFGIPEDF 16 194 TEGIPEDEDY 16 13 RTRMMEIFSE 15 41 GITAMSVKEV 15 45 MSVKEVLQSL 15 59 MVDCERIGTS 15 118 EERTRLAKEL 15 46 SVKEVLQSLV 14 53 SLVDDGMVDC 14 64 RIGTSNYYWA 14 121 TRLAKELSSL 14 146 DCEPQVVEEI 14 150 QVVEEIRQAN 14 151 VVEEIRQANK 14 193 RTFGIPEDED 14 HLA-A3 decamers 154 EI RQAN KVAK 26 129 SL RDQR EQLK 25 136 QL KAEV EKYK 25 151 VV EEIR QANK 24 25 DV FQLK DLEK 23 102 SL QKSI EKAK 22 122 RL AKEL SSLR 22 31 DL EKIA PKEK 21 172 NI FAIK SWAK 21 6 GL SAEE KRTR 20 90 ES QLSE GSQK 20 3 KK KGLS AEEK 19 15 RM MEIF SETK 19 134 RE QLKA EVEK 19 39 EK GITA MSVK 18 111 KI GRCE TEER 18 168 RW TDNI FAIK 18 68 SN YYWA FPSK 17 160 KV AKEA ANRW 17 190 KI DRTF GIPE 17 18 EI FSET KDVF 16 34 KI APKE KGIT 16 46 SV KEVL QSLV 16 53 SL VDDG MVDC 16 87 EV LESQ LSEG 16 96 GS QKHA SLQK 16 116 ET EERT RLAK 16 174 FA IKSW AKRK 16 175 AI KSWA KRKF 16 28 QL KDLE KIAP 15 59 MV DCER IGTS 15 78 AL HARK HKLE 15 150 QV VEEI RQAN 15 29 LK DLEK IAPK 14 76 SK ALHA RKHK 14 181 KR KFGF EENK 14 64 RI GTSN YYWA 13 74 FP SKAL HARK 13 85 KL EVLE SQLS 13 92 QL SEGS QKHA 13 120 RT RLAK ELSS 13 125 KE LSSL RDQR 13 HLA-B*0702 decamers 36 APKEKGITAM 20 74 FPSKALHARK 14 80 HARKHKLEVL 14 42 ITAMSVKEVL 13 114 RCETEERTRL 13 118 EERTRLAKEL 13 70 YYWAFPSKAL 12 94 SEGSQKHASL 12 20 FSETKDVFQL 11 23 TKDVFQLKDL 11 45 MSVKEVLQSL 11 77 KALHARKHKL 11 121 TRLAKELSSL 11 128 SSLRDQREQL 11 166 ANRWTDNIFA 11 84 HKLEVLESQL 10 108 EKAKIGRCET 10 148 DPQVVEEIRQ 10 118 EERTRLAKEL 26 186 FEENKIDRTF 23 10 EEKRTRMMEI 21 62 CERIGTSNYY 21 94 SEGSQKHASL 21 153 EEIRQANKVA 19 17 MEFSETKDV 16 63 ERIGTSNYYW 16 18 EIFSETKDVF 15 33 EKIAPKEKGI 15 128 SSLRDQREQL 15 135 EQLKAEVEKY 15 165 AANRWTDNIF 15 167 NRWTDNIFAI 15 170 TDNIFAIKSW 15 175 AIKSWAKRKF 15 195 FGIPEDEDYI 15 9 AEEKRTRMME 14 23 TKDVFQLKDL 14 48 KEVLQSLVDD 14 70 YYWAFPSKAL 14 77 KALHARKHKL 14 125 KELSSLRDQR 14 11 EKRTRMMEIF 13 20 FSETKDVFQL 13 21 SETKDVFQLK 13 38 KEKGITAMSV 13 115 CETEERTRLA 13 117 TEERTRLAKE 13 139 AEVEKYKDCD 13 146 DCDPQVVEEI 13 152 VEEIRQANKV 13 160 KVAKEAANRW 13 182 RKFGFEENKI 13 187 EENKIDRTFG 13 Class I nonamer analysis of amino acids 85-126 (KLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG) of 121P1F1 splice variant 1a. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. Pos 123456789 score SEQ ID NO: HLA-A*0201 nonamers 96 CCFHEIIKV 17 116 AHACNPSTL 16 107 YRKFWLGAV 15 110 FWLGAVAHA 15 HLA-A1 nonamers 98 FH EIIKVSY 26 91 SQ DPGCCFH 18 99 HE IIKVSYY 16 88 VL ESQDPGC 14 85 KL EVLESQD 11 118 AC NPSTLGG 11 HLA-A26 nonamers 87 EVLESQDPG 19 100 EIIKVSYYR 19 99 HEIIKVSYY 18 90 ESQDPGCCF 17 101 IIKVSYYRK 17 102 IKVSYYRKF 16 HLA-A3 nonamers 101 II KVSY YRK 21 85 KL EVLE SQD 19 109 KF WLGA VAH 18 111 WL GAVA HAC 17 100 EI IKVS YYR 16 99 HI IIKV SYY 14 103 KV SYYR KFW 14 108 RK FWLG AVA 14 114 AV AHAC NPS 14 87 EV LESQ DPG 13 98 FH EIIK VSY 13 116 AH ACNP STL 12 HLA-B*0702 nonamers 93 DPGCCFHEI 18 116 AHACNPSTL 13 90 ESQDPGCCF 11 106 YYRKFWLGA 11 104 VSYYRKFWL 10 108 RKFWLGAVA 10 110 FWLGAVAHA 10 HLA-B*08 nonamers 104 VSYYRKFWL 20 101 IIKVSYYRK 16 HLA-B*1510 nonamers 116 AHACNPSTL 24 98 FHEIIKVSY 14 104 VSYYRKFWL 11 102 IKVSYYRKF 10 90 ESQDPGCCF 9 HLA-B*2705 nonamers 100 EIIKVSYYR 17 101 IIKVSYYRK 15 109 KFWLGAVAH 15 95 GCCFHEIIK 14 103 IKVSYYRKF 14 99 HEIIKVSYY 13 104 VSYYRKFWL 13 116 AHACNPSTL 13 98 FHEIIKVSY 12 HLA-B*2709 nonamers 107 YRKFWLGAV 18 104 VSYYRKFWL 12 102 IKVSYYRKF 11 116 AHACNPSTL 11 HLA-B*4402 nonamers 99 HEIIKVSYY 24 116 AHACNPSTL 16 103 KVSYYRKFW 15 90 ESQDPGCCF 13 89 LESQDPGCC 12 98 FHEIIKVSY 12 102 IKVSYYRKF 12 HLA-B*5101 nonamers 93 DPGCCFHEI 25 94 PGCCFHEII 16 95 CCFHEIIKV 13 115 VAHACNPST 13 113 GAVAHACNP 12 104 VSYYRKFWL 11 107 YRKFWLGAV 11 117 HACNPSTLG 11 116 AHACNPSTL 9 Class I decamer analysis of amino acids 84-126 (HKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG) of 121P1F1 splice variant 1a. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. Pos 1234567890 score SEQ ID NO: HLA-A*0201 decamers 106 YYRKFWLGAV 17 115 VAHACNPSTL 17 94 GCCFHEIIKV 16 114 AVAHACNPST 15 103 KVSYYRKFWL 14 92 QDPGCCFHEI 13 109 KFWLGAVAHA 12 111 WLGAVAHACN 12 HLA-A*0203 decamers 107 YR KFWLGAVA 18 119 KF WLGAVAHA 18 105 SY YRKFWLGA 10 106 YY RKFWLGAV 9 108 RK FWLGAVAH 9 110 FW LGAVAHAC 9 HLA-A1 decamers 98 FH EIIKVSYY 27 91 SQ DPGCCFHE 16 97 CF HEIIKVSY 15 88 VL ESQDPGCC 12 HLA-A26 decamers 101 IIKVSYYRKF 26 100 EIIKVSYYRK 24 87 EVLESQDPGC 20 97 CFHEIIKVSY 20 103 KVSYYRKFWL 18 98 FHEIIKVSYY 15 HLA-A3 decamers 100 EIIKVS YYRK 21 108 RK FWLG AVAH 16 114 AV AHAC NPST 16 101 II KVSY YRKF 15 111 WL GAVA HACN 15 103 KV SYYR KFWL 14 85 KL EVLE SQDP 13 87 EV LESQ DPGC 13 97 CF HEII KVSY 13 HLA-B*0702 decamers 93 DPGCCFHEII 17 103 KVSYYRKFWL 13 115 VAHACNPSTL 11 106 YYRKFWLGAV 10 114 AVAHACNPST 10 HLA-B*4402 decamers 89 LESQDPGCCF 21 99 HEIIKVSYYR 13 102 IKVSYYRKFW 13 Class I nonamer analysis of amino acids 1-14 (MKCKMELSEGSQKH) of 121P1F1 splice variant 1b. Listed are those alleles and peptides in which the score fails within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. Pos 123456789 score SEQ ID NO: HLA-A1 nonamers 4 KM ELSEGSQ ta,17 10 HLA-A26 nonamers 6 ELSEGSQKH 18 HLA-A3 nonamers 5 ME LSEG SQK 21 6 EL SEGS QKH 17 HLA-B*2705 nonamers 5 MELSEGSQK 15 6 ELSEGSQKH 14 HLA-B*4402 nonamers 5 MELSEGSQK 12 Class I decamer analysis of amino acids 1-15 (MKCKMELSEGSQKHA) of 121P1F1 splice variant 1b. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the fall length 121P1F1 parental peptide sequence. Pos 1234567890 score SEQ ID NO: HLA-A*0201 decamers 6 ELSEGSQKHA 12 HLA-A*0203 decamers 6 EL SEGSQKHA 10 HLA-A26 decamers 6 ELSEGSQKHA 17 HLA-A3 decamers 4 KM ELSE GSQK 23 Class I nonamer analysis of amino acids 110-122 (AKIGRCETAKQIK) of 121P1F1 splice variant 2. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 parental peptide sequence. Pos 123456789 score SEQ ID NO: HLA-A1 nonamers 114 RC ETAKQIK 10 HLA-A3 nonamers 111 KI GRCE TAK 26 110 AK IGRC ETA 14 114 RC ETAK IIK 14 HLA-B*0702 nonamers 110 AKIGRCETA 10 HLA-B*2705 nonamers 113 GRCETAKQI 22 114 RCETAKQIK 15 111 KIGRCETAK 14 HLA-B*2709 nonamers 113 GRCETAKQI 23 HLA-B*4402 nonamers HLA-B*4402 nonamers 113 GRCETAKQ I 12 HLA-B*5101 nonamers 113 GRCETAKQI 15 Class I decamer analysis of amino acids 109-122 (KAKIGRCETAKQIK) of 121P1F1 splice variant 2. Listed are those alleles and peptides in which the score fails within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence.+TZ,1/32 Pos 1234567890 score SEQ ID NO: HLA-A*0201 decamers 111 KIGRCETAKQ 13 HLA-A*0203 decamers 109 KA KIGRCETA 10 110 AK IGRCETAK 9 HLA-A3 decamers 110 AK IGRC ETAK 20 111 KI GRCE TAKQ 17 Class I nonamer analysis of amino acids 148-164 (DPQVVEEIHNIFAIKSW) of 121P1F1 splice variant 3. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. Pos 123456789 score SEQ ID NO: HLA-A*0201 nonamers 150 QVVEEIHNI 19 HLA-A1 nonamers 152 VE EIHNIFA 16 151 VV EELHNIF 11 HLA-A26 nonamers 151 VVEEIHNIF 22 154 EIHNIFAIK 21 150 QVVEEIHNI 17 153 EEIHNIFAI 13 HLA-A3 nonamers 154 EI HNIF AIK 22 151 VV EEIH NIF 15 150 QV VEEI HNI 13 HLA-B*0702 nonamers 148 DPQVVEEIH 10 HLA-B*1510 nonamers 155 IHNIFAIKS 12 151 VVEEIHNIF 8 HLA-B*2705 nonamers 150 QVVEEIHNI 14 151 VVEEIHNIF 13 154 EIHNIFAIK 12 HLA-B*4402 nonamers 153 EEIHNIFAI 29 156 HNIFAIKSW 18 150 QVVEEIHNI 12 151 VVEEIHNIF 12 HLA-B*5101 nonamers 148 DPQVVEEIH 16 150 QVVEEIHNI 13 153 EEIHNIFAI 11 Class I decamer analysis of amino acids 147-165 (CDPQVVEEIIHNIFAIKSWA) of 121P1F1 splice variant 3. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. Pos 1234567890 score SEQ ID NO: HLA-A*0201 decamers 152 VEEIHNIFAI 13 HLA-A*0203 decamers 151 VV EEIHNIFA 10 156 HN IFAIKSWA 10 152 VE EIHNIFAI 9 HLA-A1 decamers 151 VV EEIHNIFA 16 HLA-A26 decamers 150 QVVEEIHNIF 22 154 EIHNIFAIKS 17 HLA-A3 decamers 150 QV VEEI HNIF 17 153 EE IHNI FAIK 16 HLA-B*0702 decamers 148 DPQVVEEIHN 10 HLA-B*4402 decamers 152 VEEIHNIFAI 23 153 EEIHNIFAIK 16 155 IHNIFAIKSW 15

[0853] TABLE XXVII Pos 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 Score SEQ ID NO: MHC Class II analysis of 121P1F1 for selected alleles. Listed are scores that fall within the top 50% (rounded up) of all scores for the selected alleles. HLA-DRB1*0101 15-mers 83 K H K L E V L E S Q L S E G S 31 +TL,26 86 L E V L E S Q L S E G S Q K H 30 26 V F Q L K D L E K I A P K E K 26 48 K E V L Q S L V D D G M V D C 26 67 T S N Y Y W A F P S K A L H A 25 68 S N Y Y W A F P S K A L H A R 25 141 V E K Y K D C D P Q V V E E I 25 39 E K G I T A M S V K E V L Q S 24 29 L K D L E K I A P K E K G I T 23 36 A P K E K G I T A M S V K E V 23 44 A M S V K E V L Q S L V D D G 23 167 N R W T D N I F A I K S W A K 23 13 R T R M M E I F S E T K D V F 20 24 K D V F Q L K D L E K I A P K 20 150 Q V V E E I R Q A N K V A K E 20 170 T D N I F A I K S W A K R K F 20 186 F E E N K I D R T F G I P E D 20 73 A F P S K A L H A R K H K L E 19 80 H A R K H K L E V L E S Q L S 19 116 E T E E R T R L A K E L S S L 19 173 I F A I K S W A K R K F G F E 19 33 E K I A P K E K G I T A M S V 18 138 K A E V E K Y K D C D P Q V V 18 158 A N K V A K E A A N R W T D N 18 1 M S K K K G L S A E E K R T R 17 15 R M M E I F S E T K D V F Q L 17 42 I T A M S V K E V L Q S L V D 17 65 I G T S N Y Y W A F P S K A L 17 90 E S Q L S E G S Q K H A S L Q 17 101 A S L Q K S I E K A K I G R C 17 117 T E E R T R L A K E L S S L R 17 154 E I R Q A N K V A K E A A N R 17 155 I R Q A N K V A K E A A N R W 17 16 M M E I F S E T K D V F Q L K 16 23 T K D V F Q L K D L E K I A P 16 35 I A P K E K G I T A M S V K E 16 57 D G M V D C E R I G T S N Y Y 16 HLA-DRB1*0101 15-mers 62 C E R I G T S N Y Y W A F P S 16 70 Y Y W A F P S K A L H A R K H 16 113 G R C E T E E R T R L A K E L 16 120 R T R L A K E L S S L R D Q R 16 124 A K E L S S L R D Q R E Q L K 16 127 L S S L R D Q R E Q L K A E V 16 130 L R D Q R E Q L K A E V E K Y 16 131 R D Q R E Q L K A E V E K Y K 16 188 E N K I D R T F G I P E D F D 16 190 K I D R T F G I P E D F D Y I 16 6 G L S A E E K R T R M M E I F 15 10 E E K R T R M M E I F S E T K 15 49 E V L Q S L V D D G M V D C E 15 54 L V D D G M V D C E R I G T S 15 109 K A K I G R C E T E E R T R L 15 121 T R L A K E L S S L R D Q R E 15 151 V V E E I R Q A N K V A K E A 15 HLA-DRB1*0301 (DR17) 15-mers 173 I F A I K S W A K R K F G F E 27 126 E L S S L R D Q R E Q L K A E 26 16 M M E I F S E T K D V F Q L K 25 51 L Q S L V D D G M V D C E R I 23 44 A M S V K E V L Q S L V D D G 20 148 D P Q V V E E I R Q A N K V A 20 25 D V F Q L K D L E K I A P K E 19 26 V F Q L K D L E K I A P K E K 19 127 L S S L R D Q R E Q L K A E V 19 149 P Q V V E E I R Q A N K V A K 19 152 V E E I R Q A N K V A K E A A 19 14 T R M M E I F S E T K D V F Q 18 32 L E K I A P K E K G I T A M S 18 56 D D G M V D C E R I G T S N Y 18 82 R K H K L E V L E S Q L S E G 18 90 E S Q L S E G S Q K H A S L Q 18 142 E K Y K D C D P Q V V E E I R 18 4 K K G L S A E E K R T R M M E 17 75 P S K A L H A R K H K L E V L 17 100 H A S L Q K S I E K A K I G R 17 HLA-DRB1*0301 (DR17) 15-mers 134 R E Q L K A E V E K Y K D C D 17 55 V D D G M V D C E R I G T S N 16 40 K G I T A M S V K E V L Q S T 15 112 I G R C E T E E R T R L A K E 15 181 K R K F G F E E N K I D R T F 15 175 A I K S W A K R K F G F E E N 14 19 I F S E T K D V F Q L K D L E 13 47 V K E V L Q S L V D D G M V D 13 83 K H K L E V L E S Q L S E G S 13 85 K L E V L E S Q L S E G S Q K 13 HLA-DRB1*0401 (DR4Dw4) 15-mers 68 S N Y Y W A F P S K A L H A R 28 13 R T R M M E I F S E T K D V F 26 44 A M S V K E V L Q S L V D D G 26 83 K H K L E V L E S Q L S E G S 26 148 D P Q V V E E I R Q A N K V A 26 149 P Q V V E E I R Q A N K V A K 26 170 T D N I F A I K S W A K R K F 26 67 T S N Y Y W A F P S K A L H A 22 181 K R K F G F E E N K I D R T F 22 23 T K D V F Q L K D L E K I A P 20 29 L K D L E K I A P K E K G I T 20 48 K E V L Q S L V D D G M V D C 20 56 D D G M V D C E R I G T S N Y 20 57 D G M V D C E R I G T S N Y Y 20 86 L E V L E S Q L S E G S Q K H 20 90 E S Q L S E G S Q K H A S L Q 20 120 R T R L A K E L S S L R D Q R 20 134 R E Q L K A E V E K Y K D C D 20 152 V E E I R Q A N K V A K E A A 20 5 K G L S A E E K R T R M M E I 18 72 W A F P S K A L H A R K H K L 18 106 S I E K A K I G R C E T E E R 18 112 I G R C E T E E R T R L A K E 18 113 G R C E T E E R T R L A K E L 18 126 E L S S L R D Q R E Q L K A E 18 159 N K V A K E A A N R W T D N I 18 186 F E E N K I D R T F G I P E D 18 HLA-DRB1*0401 (DR4Dw4) 15-mers 17 M E I F S E T K D V F Q L K D 16 141 V E K Y K D C D P Q V V E E I 16 166 A N R W T D N I F A I K S W A 16 183 K F G F E E N K I D R T F G I 16 4 K K G L S A E E K R T R M M E 14 14 T R M M E I F S E T K D V F Q 14 16 M M E I F S E T K D V F Q L K 14 26 V F Q L K D L E K I A P K E K 14 39 E K G I T A M S V K E V L Q S 14 51 L Q S L V D D G M V D C E R I 14 62 C E R I G T S N Y Y W A F P S 14 100 H A S L Q K S I E K A K I G R 14 104 Q K S I E K A K I G R C E T E 14 109 K A K I G R C E T E E R T R L 14 124 A K E L S S L R D Q R E Q L K 14 127 L S S L R D Q R E Q L K A E V 14 158 A N K V A K E A A N R W T D N 14 HLA-DRB1*1101 15-mers 26 V F Q L K D L E K I A P K E K 26 117 T E E R T R L A K E L S S L R 23 83 K H K L E V L E S Q L S E G S 20 155 I R Q A N K V A K E A A N R W 20 185 G F E E N K I D R T F G I P E 20 69 N Y Y W A F P S K A L H A R K 19 67 T S N Y Y W A F P S K A L H A 17 16 M M E I F S E T K D V F Q L K 16 173 I F A I K S W A K R K F G F E 16 4 K K G L S A E E K R T R M M E 15 30 K D L E K I A P K E K G I T A 15 32 L E K I A P K E K G I T A M S 15 76 S K A L H A R K H K L E V L E 15 97 S Q K H A S L Q K S I E K A K 15 101 A S L Q K S I E K A K I G R C 15 135 E Q L K A E V E K Y K D C D P 15 10 E E K R T R M M E I F S E T K 14 39 E K G I T A M S V K E V L Q S 14 48 K E V L Q S L V D D G M V D C 14 56 D D G M V D C E R I G T S N Y 14 HLA-DRB1*1101 15-mers 91 S Q L S E G S Q K H A S L Q K 14 106 S I E K A K I G R C E T E E R 14 124 A K E L S S L R D Q R E Q L K 14 148 D P Q V V E E I R Q A N K V A 14 152 V E E I R Q A N K V A K E A A 14 169 W T D N I F A I K S W A K R K 14 174 F A I K S W A K R K F G F E E 14 23 T K D V F Q L K D L E K I A P 13 42 I T A M S V K E V L Q S L V D 13 44 A M S V K E V L Q S L V D D G 13 166 A N R W T D N I F A I K S W A 13 167 N R W T D N I F A I K S W A K 13 170 T D N I F A I K S W A K R K R 13 Class II 15-mer analysis of amino acids 80-126 (HARKHKLEVLESQDPGCCFHEIIKVSYYRKFWLGAVAHACNPSTLGG) of 121P1F1 splice variant 1a. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. HLA-DRB1*0101 15-mers 83 K H K L E V L E S Q D P G C C 31 104 V S Y Y R K F W L G A V A H A 22 86 L E V L E S Q D P G C C F H E 20 103 K V S Y Y R K F W L G A V A H 20 80 H A R K H K L E V L E S Q D P 19 99 H E I I K V S Y Y R K F W L G 19 107 Y R K F W L G A V A H A C N P 19 105 S Y Y R K F W L G A V A H A C 18 108 R K F W L G A V A H A C N P S 18 106 Y Y R K F W L G A V A H A C N 17 87 E V L E S Q D P G C C F H E I 16 95 G C C F H E I I K V S Y Y R K 16 98 F H E I I K V S Y Y R K F W L 16 101 I I K V S Y Y R K F W L G A V 16 110 F W L G A V A H A C N P S T L 16 HLA-DRB1*0301 (DR17) 15-mers 95 G C C F H E I I K V S Y Y R K 24 101 I I K V S Y Y R K F W L G A V 24 99 H E I I K V S Y Y R K F W L G 20 87 E V L E S Q D P G C C F H E I 19 HLA-DRB1*0301 (DR17) 15-mers 112 L G A V A H A C N P S T L G G 16 85 K L E V L E S Q D P G C C F H 13 HLA-DRB1*0401 (DR4Dw4) 15-mers 109 K F W L G A V A H A C N P S T 26 112 L G A V A H A C N P S T L G G 26 104 V S Y Y R K F W L G A V A H A 22 83 K H K L E V L E S Q D P G C C 20 98 F H E I I K V S Y Y R K F W L 20 95 G C C G H E I I K V S Y Y R K 16 107 Y R K F W L G A V A H A C N P 16 108 R K F W L G A V A H A C N P S 16 101 I I K V S Y Y R K F W L G A V 14 HLA-DRB1*1101 15-mers 95 G C C F H E I I K V S Y Y R K 24 109 K F W L G A V A H A C N P S T 20 83 K H K L E V L E S Q D P G C C 19 103 K V S Y Y R K F W L G A V A H 16 107 Y R K F W L G A V A H A C N P 16 98 F H E I I K V S Y Y R K F W L 14 101 I I K V S Y Y R K F W L G A V 14 Class II 15-mer analysis of amino acids 1‥20 (MKCKMELSEGSQKHASLQKS) of 121P1F1 splice variant 1b. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. HLA-DRB1*0101 15-mers 2 K C K M E L S E G S Q K H A S 18 4 K M E L S E G S Q K H A S L Q 17 HLA-DRB1*0301 (DR17) 15-mers 4 K M E L S E G S Q K H A S L Q 18 HLA-DRB1*0401 (DR4Dw4) 15-mers 4 K M E L S E G S Q K H A S L Q 20 2 K C K M E L S E G S Q K H A S 14 HLA-DRB1*1101 15-mers 5 M E L S E G S Q K H A S L Q K 14 Class I 15-mer analysis of amino acids 104-122 (QKSIEKAKIGRCETAKQIK) of 121P1F1 splice variant 2. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. HLA-DRB1*0401 (DR4Dw4) 15-mers 106 S I E K A K I G R C E T A K Q 18 104 Q K S I E K A K I G R C E T A 14 HLA-DRB1*1101 15-mers 106 S I E K A K I G R C E T A K Q 14 Class II 15-mer analysis of amino acids 142-170 (EKYKDCDPQVVEEIHNIFA IKSWAKRKFG) of 121P1F1 splice variant 3. Listed are those alleles and peptides in which the score falls within the top 50% (rounded up) of the scores from the analysis of the full length 121P1F1 base peptide sequence. HLA-DRB1*0101 15-mers 152 V E E I H N I F A I K S W A K 31 149 P Q V V E E I H N I F A I K S 22 155 I H N I F A I K S W A K R K F 20 148 D P Q V V E E I H N I F A I K 17 0HLA-DRB1*0301 (DR17) 15-mers 148 D P Q V V E E I H N I F A I K 21 142 E K Y K D C D P Q V V E E I H 18 149 P Q V V E E I H N I F A I K S 17 HLA-DRB1*0401 (DR4Dw4) 15-mers 149 P Q V V E E I H N I F A I K S 26 155 I H N I F A I K S W A K R K F 26 148 D P Q V V E E I H N I F A I K 20 152 V E E I H N I F A I K S W A K 20 HLA-DRB1*1101 15-mers 152 V E E I H N I F A I K S W A K 19 149 P Q V V E E I H N I F A I K S 18 7 D P Q V V E E I H N I F A I K 15 13 E I H N I F A I K S W A K R K 14 14 I H N I F A I K S W A K R K F 13

[0854]

1 69 1 254 DNA Homo Sapiens 1 gatcacagtc tttgtatttt tctacttctg cctttagctg ttccctttgg tctcgaagtg 60 aagaaagctc ttttgctagc ctggttcgct cttccgtttc acatcggcca attttagctt 120 tctcaatgct tttctgtagg cttgcatgct tttgacttcc ctcagacaac tgagattcca 180 gaacctccaa cttatgtttc cttgcatgaa gagctttact tggaaaagcc caataataat 240 tagaagttcc gatc 254 2 867 DNA Homo Sapiens CDS (82)...(696) 2 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc c atg tca aag aaa aaa gga ctg agt gca gaa 111 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu 1 5 10 gaa aag aga act cgc atg atg gaa ata ttt tct gaa aca aaa gat gta 159 Glu Lys Arg Thr Arg Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val 15 20 25 ttt caa tta aaa gac ttg gag aag att gct ccc aaa gag aaa ggc att 207 Phe Gln Leu Lys Asp Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile 30 35 40 act gct atg tca gta aaa gaa gtc ctt caa agc tta gtt gat gat ggt 255 Thr Ala Met Ser Val Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly 45 50 55 atg gtt gac tgt gag agg atc gga act tct aat tat tat tgg gct ttt 303 Met Val Asp Cys Glu Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe 60 65 70 cca agt aaa gct ctt cat gca agg aaa cat aag ttg gag gtt ctg gaa 351 Pro Ser Lys Ala Leu His Ala Arg Lys His Lys Leu Glu Val Leu Glu 75 80 85 90 tct cag ttg tct gag gga agt caa aag cat gca agc cta cag aaa agc 399 Ser Gln Leu Ser Glu Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser 95 100 105 att gag aaa gct aaa att ggc cga tgt gaa acg gaa gag cga acc agg 447 Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg 110 115 120 cta gca aaa gag ctt tct tca ctt cga gac caa agg gaa cag cta aag 495 Leu Ala Lys Glu Leu Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys 125 130 135 gca gaa gta gaa aaa tac aaa gac tgt gat ccg caa gtt gtg gaa gaa 543 Ala Glu Val Glu Lys Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu 140 145 150 ata cgc caa gca aat aaa gta gcc aaa gaa gct gct aac aga tgg act 591 Ile Arg Gln Ala Asn Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr 155 160 165 170 gat aac ata ttc gca ata aaa tct tgg gcc aaa aga aaa ttt ggg ttt 639 Asp Asn Ile Phe Ala Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe 175 180 185 gaa gaa aat aaa att gat aga act ttt gga att cca gaa gac ttt gac 687 Glu Glu Asn Lys Ile Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp 190 195 200 tac ata gac taaaatattc catggtggtg aaggatgtac aagcttgtga 736 Tyr Ile Asp 205 atatgtaaat tttaaactat tatctaacta agtgtactga attgtcgttt gcctgtaact 796 gtgtttatca ttttattaat gttaaataaa gtgtaaaatg caaaaaaaaa aaaaaaaaaa 856 aaaaaaaaaa a 867 3 205 PRT Homo Sapiens 3 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys 145 150 155 160 Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile 165 170 175 Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp 180 185 190 Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 4 1028 DNA Homo Sapiens CDS (82)...(459) 4 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 69 ggaagcccct gcgcccgcgc c atg tca aag aaa aaa gga ctg agt gca gaa 111 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu 1 5 10 gaa aag aga act cgc atg atg gaa ata ttt tct gaa aca aaa gat gta 159 Glu Lys Arg Thr Arg Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val 15 20 25 ttt caa tta aaa gac ttg gag aag att gct ccc aaa gag aaa ggc att 207 Phe Gln Leu Lys Asp Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile 30 35 40 act gct atg tca gta aaa gaa gtc ctt caa agc tta gtt gat gat ggt 255 Thr Ala Met Ser Val Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly 45 50 55 atg gtt gac tgt gag agg atc gga act tct aat tat tat tgg gct ttt 303 Met Val Asp Cys Glu Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe 60 65 70 cca agt aaa gct ctt cat gca agg aaa cat aag ttg gag gtt ctg gaa 351 Pro Ser Lys Ala Leu His Ala Arg Lys His Lys Leu Glu Val Leu Glu 75 80 85 90 tct cag gac cct ggc tgc tgc ttc cat gaa ata att aaa gtc tcc tat 399 Ser Gln Asp Pro Gly Cys Cys Phe His Glu Ile Ile Lys Val Ser Tyr 95 100 105 tat aga aaa ttc tgg ctg ggc gca gtg gct cac gcc tgt aat ccc agc 447 Tyr Arg Lys Phe Trp Leu Gly Ala Val Ala His Ala Cys Asn Pro Ser 110 115 120 act ttg gga ggc tgaggcgggc agatcacgag gtgactttcc cccaccccca 499 Thr Leu Gly Gly 125 catgaagtgc aagatggagt tgtctgaggg aagtcaaaag catgcaagcc tacagaaaag 559 cattgagaaa gctaaaattg gccgatgtga aacggaagag cgaaccaggc tagcaaaaga 619 gctttcttca cttcgagacc aaagggaaca gctaaaggca gaagtagaaa aatacaaaga 679 ctgtgatccg caagttgtgg aagaaatacg ccaagcaaat aaagtagcca aagaagctgc 739 taacagatgg actgataaca tattcgcaat aaaatcttgg gccaaaagaa aatttgggtt 799 tgaagaaaat aaaattgata gaacttttgg aattccagaa gactttgact acatagacta 859 aaatattcca tggtggtgaa ggatgtacaa gcttgtgaat atgtaaattt taaactatta 919 tctaactaag tgtactgaat tgtcgtttgc ctgtaactgt gtttatcatt ttattaatgt 979 taaataaagt gtaaaatgca aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 1028 5 126 PRT Homo Sapiens 5 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Asp Pro Gly Cys 85 90 95 Cys Phe His Glu Ile Ile Lys Val Ser Tyr Tyr Arg Lys Phe Trp Leu 100 105 110 Gly Ala Val Ala His Ala Cys Asn Pro Ser Thr Leu Gly Gly 115 120 125 6 1028 DNA Homo Sapiens CDS (501)...(857) 6 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcaggac 360 cctggctgct gcttccatga aataattaaa gtctcctatt atagaaaatt ctggctgggc 420 gcagtggctc acgcctgtaa tcccagcact ttgggaggct gaggcgggca gatcacgagg 480 tgactttccc ccacccccac atg aag tgc aag atg gag ttg tct gag gga agt 533 Met Lys Cys Lys Met Glu Leu Ser Glu Gly Ser 1 5 10 caa aag cat gca agc cta cag aaa agc att gag aaa gct aaa att ggc 581 Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile Gly 15 20 25 cga tgt gaa acg gaa gag cga acc agg cta gca aaa gag ctt tct tca 629 Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser Ser 30 35 40 ctt cga gac caa agg gaa cag cta aag gca gaa gta gaa aaa tac aaa 677 Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr Lys 45 50 55 gac tgt gat ccg caa gtt gtg gaa gaa ata cgc caa gca aat aaa gta 725 Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys Val 60 65 70 75 gcc aaa gaa gct gct aac aga tgg act gat aac ata ttc gca ata aaa 773 Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile Lys 80 85 90 tct tgg gcc aaa aga aaa ttt ggg ttt gaa gaa aat aaa att gat aga 821 Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp Arg 95 100 105 act ttt gga att cca gaa gac ttt gac tac ata gac taaaatattc 867 Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 110 115 catggtggtg aaggatgtac aagcttgtga atatgtaaat tttaaactat tatctaacta 927 agtgtactga attgtcgttt gcctgtaact gtgtttatca ttttattaat gttaaataaa 987 gtgtaaaatg caaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 1028 7 119 PRT Homo Sapiens 7 Met Lys Cys Lys Met Glu Leu Ser Glu Gly Ser Gln Lys His Ala Ser 1 5 10 15 Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu Thr Glu 20 25 30 Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser Ser Leu Arg Asp Gln Arg 35 40 45 Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr Lys Asp Cys Asp Pro Gln 50 55 60 Val Val Glu Glu Ile Arg Gln Ala Asn Lys Val Ala Lys Glu Ala Ala 65 70 75 80 Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile Lys Ser Trp Ala Lys Arg 85 90 95 Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp Arg Thr Phe Gly Ile Pro 100 105 110 Glu Asp Phe Asp Tyr Ile Asp 115 8 752 DNA Homo Sapiens CDS (82)...(447) 8 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc c atg tca aag aaa aaa gga ctg agt gca gaa 111 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu 1 5 10 gaa aag aga act cgc atg atg gaa ata ttt tct gaa aca aaa gat gta 159 Glu Lys Arg Thr Arg Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val 15 20 25 ttt caa tta aaa gac ttg gag aag att gct ccc aaa gag aaa ggc att 207 Phe Gln Leu Lys Asp Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile 30 35 40 act gct atg tca gta aaa gaa gtc ctt caa agc tta gtt gat gat ggt 255 Thr Ala Met Ser Val Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly 45 50 55 atg gtt gac tgt gag agg atc gga act tct aat tat tat tgg gct ttt 303 Met Val Asp Cys Glu Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe 60 65 70 cca agt aaa gct ctt cat gca agg aaa cat aag ttg gag gtt ctg gaa 351 Pro Ser Lys Ala Leu His Ala Arg Lys His Lys Leu Glu Val Leu Glu 75 80 85 90 tct cag ttg tct gag gga agt caa aag cat gca agc cta cag aaa agc 399 Ser Gln Leu Ser Glu Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser 95 100 105 att gag aaa gct aaa att ggc cga tgt gaa acg gcc aag caa ata aag 447 Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu Thr Ala Lys Gln Ile Lys 110 115 120 tagccaaaga agctgctaac agatggactg ataacatatt cgcaataaaa tcttgggcca 507 aaagaaaatt tgggtttgaa gaaaataaaa ttgatagaac ttttggaatt ccagaagact 567 ttgactacat agactaaaat attccatggt ggtgaaggat gtacaagctt gtgaatatgt 627 aaattttaaa ctattatcta actaagtgta ctgaattgtc gtttgcctgt aactgtgttt 687 atcattttat taatgttaaa taaagtgtaa aatgcaaaaa aaaaaaaaaa aaaaaaaaaa 747 aaaaa 752 9 122 PRT Homo Sapiens 9 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Ala Lys Gln Ile Lys 115 120 10 822 DNA Homo Sapiens CDS (82)...(651) 10 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc c atg tca aag aaa aaa gga ctg agt gca gaa 111 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu 1 5 10 gaa aag aga act cgc atg atg gaa ata ttt tct gaa aca aaa gat gta 159 Glu Lys Arg Thr Arg Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val 15 20 25 ttt caa tta aaa gac ttg gag aag att gct ccc aaa gag aaa ggc att 207 Phe Gln Leu Lys Asp Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile 30 35 40 act gct atg tca gta aaa gaa gtc ctt caa agc tta gtt gat gat ggt 255 Thr Ala Met Ser Val Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly 45 50 55 atg gtt gac tgt gag agg atc gga act tct aat tat tat tgg gct ttt 303 Met Val Asp Cys Glu Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe 60 65 70 cca agt aaa gct ctt cat gca agg aaa cat aag ttg gag gtt ctg gaa 351 Pro Ser Lys Ala Leu His Ala Arg Lys His Lys Leu Glu Val Leu Glu 75 80 85 90 tct cag ttg tct gag gga agt caa aag cat gca agc cta cag aaa agc 399 Ser Gln Leu Ser Glu Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser 95 100 105 att gag aaa gct aaa att ggc cga tgt gaa acg gaa gag cga acc agg 447 Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg 110 115 120 cta gca aaa gag ctt tct tca ctt cga gac caa agg gaa cag cta aag 495 Leu Ala Lys Glu Leu Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys 125 130 135 gca gaa gta gaa aaa tac aaa gac tgt gat ccg caa gtt gtg gaa gaa 543 Ala Glu Val Glu Lys Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu 140 145 150 ata cat aac ata ttc gca ata aaa tct tgg gcc aaa aga aaa ttt ggg 591 Ile His Asn Ile Phe Ala Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly 155 160 165 170 ttt gaa gaa aat aaa att gat aga act ttt gga att cca gaa gac ttt 639 Phe Glu Glu Asn Lys Ile Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe 175 180 185 gac tac ata gac taaaatattc catggtggtg aaggatgtac aagcttgtga 691 Asp Tyr Ile Asp 190 atatgtaaat tttaaactat tatctaacta agtgtactga attgtcgttt gcctgtaact 751 gtgtttatca ttttattaat gttaaataaa gtgtaaaatg caaaaaaaaa aaaaaaaaaa 811 aaaaaaaaaa a 822 11 190 PRT Homo Sapiens 11 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile His Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 12 1205 DNA Homo Sapiens CDS (281)...(850) 12 gttttctgta ttgtaatatg tagagcacat tccagaactg ctcagtttcg agttacctaa 60 tggatcttca ctgtgtgcca attagtcgat ttctgtgaaa acgccccggt ttctgccaaa 120 gggcaggagt cgctgctctt gtgccgggtg ctgctggttg tgtagggcgc tgttgctttt 180 ttaaggacgc tctgcactga attaggcttc ctcgtgggtc atgatcagtt aagtcctgtc 240 aaagaaaaaa ggactgagtg cagaagaaaa gagaactcgc atg atg gaa ata ttt 295 Met Met Glu Ile Phe 1 5 tct gaa aca aaa gat gta ttt caa tta aaa gac ttg gag aag att gct 343 Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu Glu Lys Ile Ala 10 15 20 ccc aaa gag aaa ggc att act gct atg tca gta aaa gaa gtc ctt caa 391 Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys Glu Val Leu Gln 25 30 35 agc tta gtt gat gat ggt atg gtt gac tgt gag agg atc gga act tct 439 Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg Ile Gly Thr Ser 40 45 50 aat tat tat tgg gct ttt cca agt aaa gct ctt cat gca agg aaa cat 487 Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His Ala Arg Lys His 55 60 65 aag ttg gag gtt ctg gaa tct cag ttg tct gag gga agt caa aag cat 535 Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly Ser Gln Lys His 70 75 80 85 gca agc cta cag aaa agc att gag aaa gct aaa att ggc cga tgt gaa 583 Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu 90 95 100 acg gaa gag cga acc agg cta gca aaa gag ctt tct tca ctt cga gac 631 Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser Ser Leu Arg Asp 105 110 115 caa agg gaa cag cta aag gca gaa gta gaa aaa tac aaa gac tgt gat 679 Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr Lys Asp Cys Asp 120 125 130 ccg caa gtt gtg gaa gaa ata cgc caa gca aat aaa gta gcc aaa gaa 727 Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys Val Ala Lys Glu 135 140 145 gct gct aac aga tgg act gat aac ata ttc gca ata aaa tct tgg gcc 775 Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile Lys Ser Trp Ala 150 155 160 165 aaa aga aaa ttt ggg ttt gaa gaa aat aaa att gat aga act ttt gga 823 Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp Arg Thr Phe Gly 170 175 180 att cca gaa gac ttt gac tac ata gac taaaatattc catggtggtg 870 Ile Pro Glu Asp Phe Asp Tyr Ile Asp 185 190 aaggatgtac aagcttgtga atatgtaaat tttaaactat tatctaacta agtgtactga 930 attgtcgttt gcctgtaact gtgtttatca ttttattaat gttaaataaa gtgtaaaatg 990 cagatgttct tcaccccttt tggtagaaca aaagcaggat gataaccata tccccccagt 1050 gctcatcaaa gtaggacact aaaaatccat ccatctcagt caaagtcgag cggccgcgaa 1110 tttagtagta gtagcggccg ctctagagga tccaagctta cgtacgcgtg catgcgacgt 1170 catagctctt ctatagtgtc acctaaattc aagtt 1205 13 190 PRT Homo Sapiens 13 Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp 1 5 10 15 Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val 20 25 30 Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu 35 40 45 Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu 50 55 60 His Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu 65 70 75 80 Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys 85 90 95 Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu 100 105 110 Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys 115 120 125 Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn 130 135 140 Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 14 205 PRT Homo Sapiens 14 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys 145 150 155 160 Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile 165 170 175 Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp 180 185 190 Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 15 126 PRT Homo Sapiens 15 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Asp Pro Gly Cys 85 90 95 Cys Phe His Glu Ile Ile Lys Val Ser Tyr Tyr Arg Lys Phe Trp Leu 100 105 110 Gly Ala Val Ala His Ala Cys Asn Pro Ser Thr Leu Gly Gly 115 120 125 16 119 PRT Homo Sapiens 16 Met Lys Cys Lys Met Glu Leu Ser Glu Gly Ser Gln Lys His Ala Ser 1 5 10 15 Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu Thr Glu 20 25 30 Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser Ser Leu Arg Asp Gln Arg 35 40 45 Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr Lys Asp Cys Asp Pro Gln 50 55 60 Val Val Glu Glu Ile Arg Gln Ala Asn Lys Val Ala Lys Glu Ala Ala 65 70 75 80 Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile Lys Ser Trp Ala Lys Arg 85 90 95 Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp Arg Thr Phe Gly Ile Pro 100 105 110 Glu Asp Phe Asp Tyr Ile Asp 115 17 122 PRT Homo Sapiens 17 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Ala Lys Gln Ile Lys 115 120 18 190 PRT Homo Sapiens 18 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile His Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 19 190 PRT Homo Sapiens 19 Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp 1 5 10 15 Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val 20 25 30 Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu 35 40 45 Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu 50 55 60 His Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu 65 70 75 80 Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys 85 90 95 Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu 100 105 110 Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys 115 120 125 Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn 130 135 140 Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 20 205 PRT Homo Sapiens 20 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys 145 150 155 160 Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile 165 170 175 Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp 180 185 190 Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 21 205 PRT Homo Sapiens 21 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys 145 150 155 160 Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile 165 170 175 Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp 180 185 190 Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 22 205 PRT Homo Sapiens 22 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys 145 150 155 160 Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile 165 170 175 Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp 180 185 190 Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 23 205 PRT Mus musculus 23 Met Ser Lys Lys Arg Gly Leu Ser Gly Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Phe Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Leu Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys Arg Lys Leu Glu Ala Leu Asn Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Asp Leu Gln Lys Ser Ile Glu Lys Ala Arg Val 100 105 110 Gly Arg Gln Glu Thr Glu Glu Arg Ala Met Leu Ala Lys Glu Leu Phe 115 120 125 Ser Phe Arg Asp Gln Arg Gln Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Arg Glu Cys Asp Pro Gln Val Val Glu Glu Ile Arg Glu Ala Asn Lys 145 150 155 160 Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile 165 170 175 Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Ser Lys Ile Asp 180 185 190 Lys Asn Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 24 198 PRT Homo Sapiens 24 Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met Met Glu Ile Phe 1 5 10 15 Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu Glu Lys Ile Ala 20 25 30 Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys Glu Val Leu Gln 35 40 45 Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg Ile Gly Thr Ser 50 55 60 Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His Ala Arg Lys His 65 70 75 80 Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly Ser Gln Lys His 85 90 95 Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu 100 105 110 Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser Ser Leu Arg Asp 115 120 125 Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr Lys Asp Cys Asp 130 135 140 Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys Val Ala Lys Glu 145 150 155 160 Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile Lys Ser Trp Ala 165 170 175 Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp Arg Thr Phe Gly 180 185 190 Ile Pro Glu Asp Phe Asp 195 25 200 PRT Schizosaccharomyces pombe 25 Lys Gly Leu Ser Leu Ala Glu Lys Arg Arg Arg Leu Glu Ala Ile Phe 1 5 10 15 His Asp Ser Lys Asp Phe Phe Gln Leu Lys Glu Val Glu Lys Leu Gly 20 25 30 Ser Lys Lys Gln Ile Val Leu Gln Thr Val Lys Asp Val Leu Gln Ser 35 40 45 Leu Val Asp Asp Asn Ile Val Lys Thr Glu Lys Ile Gly Thr Ser Asn 50 55 60 Tyr Tyr Trp Ser Phe Pro Ser Asp Ala Lys Arg Ser Arg Glu Ser Val 65 70 75 80 Leu Gly Ser Leu Gln Ala Gln Leu Asp Asp Leu Lys Gln Lys Ser Lys 85 90 95 Thr Leu Asp Glu Asn Ile Ser Phe Glu Lys Ser Lys Arg Asp Asn Glu 100 105 110 Gly Thr Glu Asn Asp Ala Asn Gln Tyr Thr Leu Glu Leu Leu His Ala 115 120 125 Lys Glu Ser Glu Leu Lys Leu Leu Lys Thr Gln Leu Ser Asn Leu Asn 130 135 140 His Cys Asn Pro Glu Thr Phe Glu Leu Lys Asn Glu Asn Thr Lys Lys 145 150 155 160 Tyr Met Glu Ala Ala Asn Leu Trp Thr Asp Gln Ile His Thr Leu Ile 165 170 175 Ala Phe Cys Arg Asp Met Gly Ala Asp Thr Asn Gln Ile Arg Glu Tyr 180 185 190 Cys Ser Ile Pro Glu Asp Leu Asp 195 200 26 14 PRT Clostridiumn toxi 26 Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu 1 5 10 27 21 PRT Plasmodium falciparum 27 Asp Ile Glu Lys Lys Ile Ala Lys Met Glu Lys Ala Ser Ser Val Phe 1 5 10 15 Asn Val Val Asn Ser 20 28 16 PRT Streptococcus aureus 28 Gly Ala Val Asp Ser Ile Leu Gly Gly Val Ala Thr Tyr Gly Ala Ala 1 5 10 15 29 13 PRT Artificial Sequence Artificially Synthesized Peptide 29 Xaa Lys Xaa Val Ala Ala Trp Thr Leu Lys Ala Ala Xaa 1 5 10 30 43 DNA Homo Sapiens 30 ttttgatcaa gctttttttt tttttttttt tttttttttt ttt 43 31 42 DNA Homo Sapiens 31 ctaatacgac tcactatagg gctcgagcgg ccgcccgggc ag 42 32 12 DNA Homo Sapiens 32 gatcctgccc gg 12 33 40 DNA Homo Sapiens 33 gtaatacgac tcactatagg gcagcgtggt cgcggccgag 40 34 10 DNA Homo Sapiens 34 gatcctcggc 10 35 22 DNA Homo Sapiens 35 ctaatacgac tcactatagg gc 22 36 22 DNA Homo Sapiens 36 tcgagcggcc gcccgggcag ga 22 37 20 DNA Homo Sapiens 37 agcgtggtcg cggccgagga 20 38 25 DNA Homo Sapiens 38 atatcgccgc gctcgtcgtc gacaa 25 39 26 DNA Homo Sapiens 39 agccacacgc agctcattgt agaagg 26 40 24 DNA Homo Sapiens 40 gattacaagg atgacgacga taag 24 41 1028 DNA Homo Sapiens 41 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcaggac 360 cctggctgct gcttccatga aataattaaa gtctcctatt atagaaaatt ctggctgggc 420 gcagtggctc acgcctgtaa tcccagcact ttgggaggct gaggcgggca gatcacgagg 480 tgactttccc ccacccccac atgaagtgca agatggagtt gtctgaggga agtcaaaagc 540 atgcaagcct acagaaaagc attgagaaag ctaaaattgg ccgatgtgaa acggaagagc 600 gaaccaggct agcaaaagag ctttcttcac ttcgagacca aagggaacag ctaaaggcag 660 aagtagaaaa atacaaagac tgtgatccgc aagttgtgga agaaatacgc caagcaaata 720 aagtagccaa agaagctgct aacagatgga ctgataacat attcgcaata aaatcttggg 780 ccaaaagaaa atttgggttt gaagaaaata aaattgatag aacttttgga attccagaag 840 actttgacta catagactaa aatattccat ggtggtgaag gatgtacaag cttgtgaata 900 tgtaaatttt aaactattat ctaactaagt gtactgaatt gtcgtttgcc tgtaactgtg 960 tttatcattt tattaatgtt aaataaagtg taaaatgcaa aaaaaaaaaa aaaaaaaaaa 1020 aaaaaaaa 1028 42 869 DNA Homo Sapiens 42 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagagt 360 tgtctgaggg aagtcaaaag catgcaagcc tacagaaaag cattgagaaa gctaaaattg 420 gccgatgtga aacggaagag cgaaccaggc tagcaaaaga gctttcttca cttcgagacc 480 aaagggaaca gctaaaggca gaagtagaaa aatacaaaga ctgtgatccg caagttgtgg 540 aagaaatacg ccaagcaaat aaagtagcca aagaagctgc taacagatgg actgataaca 600 tattcgcaat aaaatcttgg gccaaaagaa aatttgggtt tgaagaaaat aaaattgata 660 gaacttttgg aattccagaa gactttgact acatagacta aaatattcca tggtggtgaa 720 ggatgtacaa gcttgtgaat atgtaaattt taaactatta tctaactaag tgtactgaat 780 tgtcgtttgc ctgtaactgt gtttatcatt ttattaatgt taaataaagt gtaaaatgca 840 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 869 43 869 DNA Homo Sapiens 43 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagagt 360 tgtctgaggg aagtcaaaag catgcaagcc tacagaaaag cattgagaaa gctaaaattg 420 gccgatgtga aacggaagag cgaaccaggc tagcaaaaga gctttcttca cttcgagacc 480 aaagggaaca gctaaaggca gaagtagaaa aatacaaaga ctgtgatccg caagttgtgg 540 aagaaatacg ccaagcaaat aaagtagcca aagaagctgc taacagatgg actgataaca 600 tattcgcaat aaaatcttgg gccaaaagaa aatttgggtt tgaagaaaat aaaattgata 660 gaacttttgg aattccagaa gactttgact acatagacta aaatattcca tggtggtgaa 720 ggatgtacaa gcttgtgaat atgtaaattt taaactatta tctaactaag tgtactgaat 780 tgtcgtttgc ctgtaactgt gtttatcatt ttattaatgt taaataaagt gtaaaatgca 840 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 869 44 206 PRT Homo Sapiens 44 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Gln Leu Ser Glu 85 90 95 Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys 100 105 110 Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu 115 120 125 Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys 130 135 140 Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn 145 150 155 160 Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala 165 170 175 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 180 185 190 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 45 206 PRT Homo Sapiens 45 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Glu Leu Ser Glu 85 90 95 Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys 100 105 110 Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu 115 120 125 Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys 130 135 140 Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn 145 150 155 160 Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala 165 170 175 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 180 185 190 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 46 126 PRT Homo Sapiens 46 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Asp Pro Gly Cys 85 90 95 Cys Phe His Glu Ile Ile Lys Val Ser Tyr Tyr Arg Lys Phe Trp Leu 100 105 110 Gly Ala Val Ala His Ala Cys Asn Pro Ser Thr Leu Gly Gly 115 120 125 47 119 PRT Homo Sapiens 47 Met Lys Cys Lys Met Glu Leu Ser Glu Gly Ser Gln Lys His Ala Ser 1 5 10 15 Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile Gly Arg Cys Glu Thr Glu 20 25 30 Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser Ser Leu Arg Asp Gln Arg 35 40 45 Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr Lys Asp Cys Asp Pro Gln 50 55 60 Val Val Glu Glu Ile Arg Gln Ala Asn Lys Val Ala Lys Glu Ala Ala 65 70 75 80 Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile Lys Ser Trp Ala Lys Arg 85 90 95 Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp Arg Thr Phe Gly Ile Pro 100 105 110 Glu Asp Phe Asp Tyr Ile Asp 115 48 752 DNA Homo Sapiens 48 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cggccaagca aataaagtag ccaaagaagc tgctaacaga tggactgata 480 acatattcgc aataaaatct tgggccaaaa gaaaatttgg gtttgaagaa aataaaattg 540 atagaacttt tggaattcca gaagactttg actacataga ctaaaatatt ccatggtggt 600 gaaggatgta caagcttgtg aatatgtaaa ttttaaacta ttatctaact aagtgtactg 660 aattgtcgtt tgcctgtaac tgtgtttatc attttattaa tgttaaataa agtgtaaaat 720 gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 752 49 433 DNA Homo Sapiens 49 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cgg 433 50 433 DNA Homo Sapiens 50 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cgg 433 51 320 DNA Homo Sapiens 51 gccaagcaaa taaagtagcc aaagaagctg ctaacagatg gactgataac atattcgcaa 60 taaaatcttg ggccaaaaga aaatttgggt ttgaagaaaa taaaattgat agaacttttg 120 gaattccaga agactttgac tacatagact aaaatattcc atggtggtga aggatgtaca 180 agcttgtgaa tatgtaaatt ttaaactatt atctaactaa gtgtactgaa ttgtcgtttg 240 cctgtaactg tgtttatcat tttattaatg ttaaataaag tgtaaaatgc aaaaaaaaaa 300 aaaaaaaaaa aaaaaaaaaa 320 52 320 DNA Homo Sapiens 52 gccaagcaaa taaagtagcc aaagaagctg ctaacagatg gactgataac atattcgcaa 60 taaaatcttg ggccaaaaga aaatttgggt ttgaagaaaa taaaattgat agaacttttg 120 gaattccaga agactttgac tacatagact aaaatattcc atggtggtga aggatgtaca 180 agcttgtgaa tatgtaaatt ttaaactatt atctaactaa gtgtactgaa ttgtcgtttg 240 cctgtaactg tgtttatcat tttattaatg ttaaataaag tgtaaaatgc aaaaaaaaaa 300 aaaaaaaaaa aaaaaaaaaa 320 53 122 PRT Homo Sapiens 53 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg 115 120 54 122 PRT Homo Sapiens 54 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Ala Lys Gln Ile Lys 115 120 55 122 PRT Homo Sapiens 55 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Ala Lys Gln Ile Lys 115 120 56 822 DNA Homo Sapiens 56 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cggaagagcg aaccaggcta gcaaaagagc tttcttcact tcgagaccaa 480 agggaacagc taaaggcaga agtagaaaaa tacaaagact gtgatccgca agttgtggaa 540 gaaatacata acatattcgc aataaaatct tgggccaaaa gaaaatttgg gtttgaagaa 600 aataaaattg atagaacttt tggaattcca gaagactttg actacataga ctaaaatatt 660 ccatggtggt gaaggatgta caagcttgtg aatatgtaaa ttttaaacta ttatctaact 720 aagtgtactg aattgtcgtt tgcctgtaac tgtgtttatc attttattaa tgttaaataa 780 agtgtaaaat gcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 822 57 547 DNA Homo Sapiens 57 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cggaagagcg aaccaggcta gcaaaagagc tttcttcact tcgagaccaa 480 agggaacagc taaaggcaga agtagaaaaa tacaaagact gtgatccgca agttgtggaa 540 gaaatac 547 58 547 DNA Homo Sapiens 58 ccaaaatcaa acgcgtccgg gcctgtcccg cccctctccc caagcgcggg cccggccagc 60 ggaagcccct gcgcccgcgc catgtcaaag aaaaaaggac tgagtgcaga agaaaagaga 120 actcgcatga tggaaatatt ttctgaaaca aaagatgtat ttcaattaaa agacttggag 180 aagattgctc ccaaagagaa aggcattact gctatgtcag taaaagaagt ccttcaaagc 240 ttagttgatg atggtatggt tgactgtgag aggatcggaa cttctaatta ttattgggct 300 tttccaagta aagctcttca tgcaaggaaa cataagttgg aggttctgga atctcagttg 360 tctgagggaa gtcaaaagca tgcaagccta cagaaaagca ttgagaaagc taaaattggc 420 cgatgtgaaa cggaagagcg aaccaggcta gcaaaagagc tttcttcact tcgagaccaa 480 agggaacagc taaaggcaga agtagaaaaa tacaaagact gtgatccgca agttgtggaa 540 gaaatac 547 59 275 DNA Homo Sapiens 59 ataacatatt cgcaataaaa tcttgggcca aaagaaaatt tgggtttgaa gaaaataaaa 60 ttgatagaac ttttggaatt ccagaagact ttgactacat agactaaaat attccatggt 120 ggtgaaggat gtacaagctt gtgaatatgt aaattttaaa ctattatcta actaagtgta 180 ctgaattgtc gtttgcctgt aactgtgttt atcattttat taatgttaaa taaagtgtaa 240 aatgcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 275 60 275 DNA Homo Sapiens 60 ataacatatt cgcaataaaa tcttgggcca aaagaaaatt tgggtttgaa gaaaataaaa 60 ttgatagaac ttttggaatt ccagaagact ttgactacat agactaaaat attccatggt 120 ggtgaaggat gtacaagctt gtgaatatgt aaattttaaa ctattatcta actaagtgta 180 ctgaattgtc gtttgcctgt aactgtgttt atcattttat taatgttaaa taaagtgtaa 240 aatgcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 275 61 205 PRT Homo Sapiens 61 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn Lys 145 150 155 160 Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala Ile 165 170 175 Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile Asp 180 185 190 Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 195 200 205 62 190 PRT Homo Sapiens 62 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile His Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 63 190 PRT Homo Sapiens 63 Met Ser Lys Lys Lys Gly Leu Ser Ala Glu Glu Lys Arg Thr Arg Met 1 5 10 15 Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp Leu 20 25 30 Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val Lys 35 40 45 Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu Arg 50 55 60 Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu His 65 70 75 80 Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu Gly 85 90 95 Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys Ile 100 105 110 Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu Ser 115 120 125 Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys Tyr 130 135 140 Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile His Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 64 1205 DNA Homo Sapiens 64 gttttctgta ttgtaatatg tagagcacat tccagaactg ctcagtttcg agttacctaa 60 tggatcttca ctgtgtgcca attagtcgat ttctgtgaaa acgccccggt ttctgccaaa 120 gggcaggagt cgctgctctt gtgccgggtg ctgctggttg tgtagggcgc tgttgctttt 180 ttaaggacgc tctgcactga attaggcttc ctcgtgggtc atgatcagtt aagtcctgtc 240 aaagaaaaaa ggactgagtg cagaagaaaa gagaactcgc atgatggaaa tattttctga 300 aacaaaagat gtatttcaat taaaagactt ggagaagatt gctcccaaag agaaaggcat 360 tactgctatg tcagtaaaag aagtccttca aagcttagtt gatgatggta tggttgactg 420 tgagaggatc ggaacttcta attattattg ggcttttcca agtaaagctc ttcatgcaag 480 gaaacataag ttggaggttc tggaatctca gttgtctgag ggaagtcaaa agcatgcaag 540 cctacagaaa agcattgaga aagctaaaat tggccgatgt gaaacggaag agcgaaccag 600 gctagcaaaa gagctttctt cacttcgaga ccaaagggaa cagctaaagg cagaagtaga 660 aaaatacaaa gactgtgatc cgcaagttgt ggaagaaata cgccaagcaa ataaagtagc 720 caaagaagct gctaacagat ggactgataa catattcgca ataaaatctt gggccaaaag 780 aaaatttggg tttgaagaaa ataaaattga tagaactttt ggaattccag aagactttga 840 ctacatagac taaaatattc catggtggtg aaggatgtac aagcttgtga atatgtaaat 900 tttaaactat tatctaacta agtgtactga attgtcgttt gcctgtaact gtgtttatca 960 ttttattaat gttaaataaa gtgtaaaatg cagatgttct tcaccccttt tggtagaaca 1020 aaagcaggat gataaccata tccccccagt gctcatcaaa gtaggacact aaaaatccat 1080 ccatctcagt caaagtcgag cggccgcgaa tttagtagta gtagcggccg ctctagagga 1140 tccaagctta cgtacgcgtg catgcgacgt catagctctt ctatagtgtc acctaaattc 1200 aagtt 1205 65 756 DNA Homo Sapiens 65 tgtcaaagaa aaaaggactg agtgcagaag aaaagagaac tcgcatgatg gaaatatttt 60 ctgaaacaaa agatgtattt caattaaaag acttggagaa gattgctccc aaagagaaag 120 gcattactgc tatgtcagta aaagaagtcc ttcaaagctt agttgatgat ggtatggttg 180 actgtgagag gatcggaact tctaattatt attgggcttt tccaagtaaa gctcttcatg 240 caaggaaaca taagttggag gttctggaat ctcagttgtc tgagggaagt caaaagcatg 300 caagcctaca gaaaagcatt gagaaagcta aaattggccg atgtgaaacg gaagagcgaa 360 ccaggctagc aaaagagctt tcttcacttc gagaccaaag ggaacagcta aaggcagaag 420 tagaaaaata caaagactgt gatccgcaag ttgtggaaga aatacgccaa gcaaataaag 480 tagccaaaga agctgctaac agatggactg ataacatatt cgcaataaaa tcttgggcca 540 aaagaaaatt tgggtttgaa gaaaataaaa ttgatagaac ttttggaatt ccagaagact 600 ttgactacat agactaaaat attccatggt ggtgaaggat gtacaagctt gtgaatatgt 660 aaattttaaa ctattatcta actaagtgta ctgaattgtc gtttgcctgt aactgtgttt 720 atcattttat taatgttaaa taaagtgtaa aatgca 756 66 756 DNA Homo Sapiens 66 tgtcaaagaa aaaaggactg agtgcagaag aaaagagaac tcgcatgatg gaaatatttt 60 ctgaaacaaa agatgtattt caattaaaag acttggagaa gattgctccc aaagagaaag 120 gcattactgc tatgtcagta aaagaagtcc ttcaaagctt agttgatgat ggtatggttg 180 actgtgagag gatcggaact tctaattatt attgggcttt tccaagtaaa gctcttcatg 240 caaggaaaca taagttggag gttctggaat ctcagttgtc tgagggaagt caaaagcatg 300 caagcctaca gaaaagcatt gagaaagcta aaattggccg atgtgaaacg gaagagcgaa 360 ccaggctagc aaaagagctt tcttcacttc gagaccaaag ggaacagcta aaggcagaag 420 tagaaaaata caaagactgt gatccgcaag ttgtggaaga aatacgccaa gcaaataaag 480 tagccaaaga agctgctaac agatggactg ataacatatt cgcaataaaa tcttgggcca 540 aaagaaaatt tgggtttgaa gaaaataaaa ttgatagaac ttttggaatt ccagaagact 600 ttgactacat agactaaaat attccatggt ggtgaaggat gtacaagctt gtgaatatgt 660 aaattttaaa ctattatcta actaagtgta ctgaattgtc gtttgcctgt aactgtgttt 720 atcattttat taatgttaaa taaagtgtaa aatgca 756 67 190 PRT Homo Sapiens 67 Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp 1 5 10 15 Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val 20 25 30 Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu 35 40 45 Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu 50 55 60 His Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu 65 70 75 80 Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys 85 90 95 Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu 100 105 110 Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys 115 120 125 Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn 130 135 140 Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 68 190 PRT Homo Sapiens 68 Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp 1 5 10 15 Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val 20 25 30 Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu 35 40 45 Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu 50 55 60 His Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu 65 70 75 80 Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys 85 90 95 Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu 100 105 110 Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys 115 120 125 Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn 130 135 140 Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 69 190 PRT Homo Sapiens 69 Met Met Glu Ile Phe Ser Glu Thr Lys Asp Val Phe Gln Leu Lys Asp 1 5 10 15 Leu Glu Lys Ile Ala Pro Lys Glu Lys Gly Ile Thr Ala Met Ser Val 20 25 30 Lys Glu Val Leu Gln Ser Leu Val Asp Asp Gly Met Val Asp Cys Glu 35 40 45 Arg Ile Gly Thr Ser Asn Tyr Tyr Trp Ala Phe Pro Ser Lys Ala Leu 50 55 60 His Ala Arg Lys His Lys Leu Glu Val Leu Glu Ser Gln Leu Ser Glu 65 70 75 80 Gly Ser Gln Lys His Ala Ser Leu Gln Lys Ser Ile Glu Lys Ala Lys 85 90 95 Ile Gly Arg Cys Glu Thr Glu Glu Arg Thr Arg Leu Ala Lys Glu Leu 100 105 110 Ser Ser Leu Arg Asp Gln Arg Glu Gln Leu Lys Ala Glu Val Glu Lys 115 120 125 Tyr Lys Asp Cys Asp Pro Gln Val Val Glu Glu Ile Arg Gln Ala Asn 130 135 140 Lys Val Ala Lys Glu Ala Ala Asn Arg Trp Thr Asp Asn Ile Phe Ala 145 150 155 160 Ile Lys Ser Trp Ala Lys Arg Lys Phe Gly Phe Glu Glu Asn Lys Ile 165 170 175 Asp Arg Thr Phe Gly Ile Pro Glu Asp Phe Asp Tyr Ile Asp 180 185 190 

1. A composition comprising: a substance that modulates the status of 121P1F1, or a molecule that is modulated by 121P1F1 whereby the status of a cell that expresses 121P1F1 is modulated:
 2. The composition of claim 1, further comprising a pharmaceutically acceptable carrier.
 3. A pharmaceutical composition that comprises the composition of claim 1 in a human unit dose form.
 4. A composition of claim 1 wherein the substance comprises an antibody or fragment thereof that specifically binds to a 121P1F1-related protein.
 5. The antibody or fragment thereof of claim 4, which is monoclonal.
 6. A recombinant protein comprising an antigen-binding region of a monoclonal antibody of claim
 5. 7. The antibody or fragment thereof of claim 4, which is labeled with a detectable marker.
 8. The recombinant protein of claim 6, which is labeled with a detectable marker.
 9. The antibody fragment of an antibody of claim 4, which is an Fab, F(ab′)2, Fv or sFv fragment.
 10. The antibody of claim 4, which is a human antibody, a humanized antibody or a chimeric antibody.
 11. A non-human transgenic animal that produces an antibody of claim
 4. 12. A hybridoma that produces an antibody of claim
 5. 13. A single chain monoclonal antibody that immunospecifically binds to a 121P1F1-related protein, and that comprises the variable domains of the heavy and light chains of a monoclonal antibody of claim
 5. 14. A vector comprising a polynucleotide that encodes a single chain monoclonal antibody of claim
 13. 15. A method of delivering a cytotoxic agent or a diagnostic agent to a cell that expresses 121P1F1. said method comprising: providing the cytotoxic agent or the diagnostic agent conjugated to an antibody or fragment thereof of claim 4; and, exposing the cell to the antibody-agent or fragment-agent conjugate.
 16. A composition of claim 1 wherein the substance comprises a polynucleotide that encodes an antibody or fragment thereof either of which immunospecifically binds to an 121P1F1-related protein.
 17. A composition of claim 3 wherein the substance comprises a 121P1F1-related protein.
 18. The composition of claim 17, further comprising antigen presenting cells.
 19. The composition of claim 1 wherein the substance comprises an analog of a peptide of eight, nine, ten, or eleven contiguous amino acids of FIG. 2A, FIG. 2B, FIG. 2C, FIG. 2D, FIG. 2E, or FIG. 2F (SEQ ID NO: ______).
 20. A composition of claim 1 wherein the substance comprises a CTL polypeptide epitope of the amino acid sequence of FIG. 2A, FIG. 2B, FIG. 2C, FIG. 2D, FIG. 2E, or FIG. 2F (SEQ ID NO: ______), with a proviso that the epitope is not the entire amino acid sequence of FIG. 2A (SEQ ID NO: ______).
 21. The composition of claim 20 wherein the CTL epitope comprises a polypeptide selected from Tables V-XVIII, XXVI, and XXVII, with a proviso that the epitope is not the entire amino acid sequence of FIG. 2A (SEQ ID NO: ______).
 22. A composition of claim 1 wherein the substance comprises an antibody polypeptide epitope of the amino acid sequence of FIG. 2A, FIG. 2B, FIG. 2C, FIG. 2D, FIG. 2E, or FIG. 2F (SEQ ID NO: ______), with a proviso that the epitope is not the entire amino acid sequence of FIG. 2A (SEQ ID NO: ______).
 23. A composition of claim 22 wherein the antibody epitope comprises a peptide region of at least 5 amino acids of FIG. 2A (SEQ ID NO: L in any whole number increment up to 205 that includes an amino acid position selected from: an amino acid position having a value greater than 0.5 in the Hydrophilicity profile of FIG. 5A, an amino acid position having a value less than 0.5 in the Hydropathicity profile of FIG. 6A, an amino acid position having a value greater than 0.5 in the Percent Accessible Residues profile of FIG. 7A: an amino acid position having a value greater than 0.5 in the Average Flexibility profile on FIG. 8A; or an amino acid position having a value greater than 0.5 in the Beta-turn profile of FIG. 9A. with a proviso that the epitope is not the entire amino acid sequence of FIG. 2A (SEQ ID NO: ______).
 24. The recombinant protein of claim 23, which comprises murine antigen binding region residues and human constant region residues.
 25. A polynucleotide that encodes an analog peptide of claim
 19. 26. A composition of claim 1 wherein the substance comprises a polynucleotide that comprises an 121P1F1-related protein coding sequence, with a Droviso that the coding sequence does not encode the entire amino acid sequence of FIG. 2A (SEQ ID NO: ______).
 27. The composition of claim 26 in human unit dose form.
 28. A composition of claim 26 comprising a polynucleotide from position number 82 through number 696 of FIG. 2A (SEQ ID NO: ______) followed by a stop codon.
 29. The composition of claim 28 wherein T is substituted with U.
 30. A composition of claim 32 that comprises the coding sequence for the polynucleotide of FIG. 2A (SEQ ID NO: ______).
 31. The composition of claim 30 wherein T is substituted with U.
 32. A composition of claim 26 comprising a polynucleotide that encodes an 121P1F1-related protein that is at least 90% homologous to the entire amino acid sequence shown in FIG. 2A, FIG. 2B, FIG. 2C, FIG. 2D, FIG. 2E, or FIG. 2F (SEQ ID NO: ______).
 33. The composition of claim 32 wherein the polynucleotide encodes an 121P1F1-related protein that is at least 90% identical to the entire amino acid sequence shown in FIG. 2A, FIG. 2B, FIG. 2C, FIG. 2D, FIG. 2E, or FIG. 2F (SEQ ID NO: ______).
 34. A composition of claim 26 wherein the substance comprises a polynucleotide that encodes at least one peptide set forth in Tables V-XVIII, XXVI. and XXVII, with a proviso that the entire amino acid sequence of FIG. 2A is not encoded.
 35. A composition of claim 26 comprising a polynucleotide that encodes a peptide region of at least 5 amino acids of FIG. 2A (SEQ ID NO: ______) that includes an amino acid position selected from: an amino acid position having a value greater than 0.5 in the Hydrophilicity profile of FIG. 5A, an amino acid position having a value less than 0.5 in the Hydropathicity profile of FIG. 6A; an amino acid position having a value greater than 0.5 in the Percent Accessible Residues profile of FIG. 7A; an amino acid position having a value greater than 0.5 in the Average Flexibility profile on FIG. 8A; or an amino acid position having a value greater than 0.5 in the Beta-turn profile of FIG. 9A, with a proviso that the entire amino acid sequence of FIG. 2A (SEQ ID NO: ______) is not encoded.
 36. A composition comprising a polynucleotide that is filly complementary to a polynucleotide of claim
 26. 37. A composition comprising a polynucleotide that is filly complementary to a polynucleotide of claim
 28. 38. A composition comprising a polynucleotide that is fully complementary to a polynucleotide of claim
 29. 39. A composition comprising a polynucleotide that is fully complementary to a polynucleotide of claim 30, in human unit dose form.
 40. A composition comprising a polynucleotide that is fully complementary to a polynucleotide of claim
 31. 41. A composition comprising a polynucleotide that is fully complementary to a polynucleotide of claim
 32. 42. A composition comprising a polynucleotide that is fully complementary to a polynucleotide of claim
 33. 43. A composition comprising a polynucleotide that is fully complementary to a polynucleotide of claim
 34. 44. A pharmaceutical composition of claim 1 wherein the substance comprises a ribozyme that cleaves a polynucleotide having 121P1F1 coding sequence and a physiologically acceptable carrier.
 45. A pharmaceutical composition of claim 1 wherein the substance comprises a nucleic acid molecule that encodes a ribozyme that cleaves a polynucleotide having 121P1F1 coding sequence and a physiologically acceptable carrier.
 46. A pharmaceutical composition of claim 1 wherein the substance comprises human T cells, wherein said T cells specifically recognize a 121P1F1 peptide sequence in the context of a particular HLA molecule.
 47. A method of inhibiting growth of cancer cells that expresses 121P1F1, the method comprising: administering to the cells the composition of claim
 1. 48. A method of claim 47 of inhibiting growth of cancer cells that express 121P1F1, the method comprising steps of: administering to said cells an antibody or fragment thereof either of which specifically bind to a 121P1F1-related protein.
 49. A method of claim 47 of inhibiting growth of cancer cells that express 121P1F1, the method comprising steps of: administering to said cells a vector that encodes a single chain monoclonal antibody that immunospecifically binds to an 121P1F1-related protein.
 50. A method of claim 47 of inhibiting growth of cancer cells that express 121P1F1, the method comprising steps of: administering to said cells an 121P1F1-related protein.
 51. A method of claim 47 of inhibiting growth of cancer cells that express 121P1F1, the method comprising steps of: administering to said cells a vector that comprises a polynucleotide comprising a 121P1F1-related protein coding sequence.
 52. A method of claim 47 of inhibiting growth of cancer cells that express 121P1F1, the method comprising steps of: administering to said cells an antisense polynucleotide complementary to a polynucleotide having a 121P1F1 coding bi sequence.
 53. A method of claim 47 of inhibiting growth of cancer cells that express 121P1F1, the method comprising steps of: administering to said cells a ribozyme that cleaves a polynucleotide having 121P1F1 coding sequence.
 54. A method of claim 47 of inhibiting growth of cancer cells that express 121P1F1 and a particular HLA molecule, the method comprising steps of: administering to said cells human T cells, wherein said T cells specifically recognize an 121P1F1 peptide sequence in the context of the particular HLA molecule.
 55. A method of treating a patient who bears cancer cells that express 121P1F1. the method comprising: administering to the patient the composition of claim
 1. 56. A method of claim 55 for treating a patient who bears cancer cells that expresses 121P1F1 the method comprising steps of: administering to said patient an antibody or fragment thereof either of which specifically binds to a 121P1F1-related protein.
 57. A method of claim 55 for treating a patient who bears cancer cells that expresses 121P1F1, the method comprising steps of: administering to said patient a vector that encodes an antibody or fragment thereof either of which immunospecifically bind to an 121P1F1-related protein.
 58. A method of claim 57 for treating a patient with a cancer that expresses 121P1F1, the method comprising steps of: administering to said patient a vector that delivers a single chain monoclonal antibody coding sequence, whereby the encoded single chain antibody is expressed intracellularly within cancer cells that express 121P1F1.
 59. A method of claim 55 for treating a patient who bears cancer cells that express 121P1F1, the method comprising steps of: administering to said patient an 121P1F1-related protein.
 60. A method of claim 55 for treating a patient who bears cancer cells that express 121P1F1, the method comprising steps of: administering to said patient a vector that comprises a polynucleotide comprising a 121P1F1-related protein coding sequence.
 61. A method of claim 55 for treating a patient who bears cancer cells that express 121P1F1, the method comprising steps of: administering to said patient an antisense polynucleotide complementary to a polynucleotide having a 121P1F1 coding sequence.
 62. A method of claim 55 for treating a patient who bears cancer cells that express 121P1F1, the method comprising steps of: administering to said patient a ribozyme that cleaves a polynucleotide having an 121P1F1 coding sequence.
 63. A method of claim 55 for treating a patient who bears cancer cells that express 121P1F1, the method comprising steps of: administering to said patient a nucleic acid molecule that encodes a ribozyme that cleaves a polynucleotide having an 121P1F1 coding sequence.
 64. A method of claim 55 for treating a patient who bears cancer cells that express 121P1F1 and a particular HLA molecule, the method comprising steps of: administering to said patient human T cells, wherein said T cells specifically recognize an 121P1F1 peptide sequence in the context of the particular HLA molecule.
 65. A method of generating a mammalian immune response directed to 121P1F1, the method comprising: exposing cells of the mammal's immune system to an immunogenic portion of a) an 121P1F1-related protein and/or b) a nucleotide sequence that encodes said protein, whereby an immune response is generated to 121P1F1.
 66. A method of inducing an immune response of claim 65, said method comprising: providing a 121P1F1-related protein that comprises at least one T cell or at least one B cell epitope; contacting the epitope with a mammalian immune system T cell or B cell respectively, whereby the T cell or B cell is induced.
 67. The method of claim 66 wherein the immune system cell is a B cell, whereby the induced B cell generates antibodies that specifically bind to the 121P1F1-related protein.
 68. The method of claim 66 wherein the immune system cell is a T cell that is a cytotoxic T cell (CTL), whereby the activated CTL kills an autologous cell that expresses the 121P1F1-related protein.
 69. The method of claim 66 wherein the immune system cell is a T cell that is a helper T cell (HTL), whereby the activated HTL secretes cytokines that facilitate the cytotoxic activity of a cytotoxic T cell (CTL) or the antibody producing activity of a B cell.
 70. An assay for detecting the presence of a 121P1F1-related protein or polynucleotide in a biological sample from a patient who has or who is suspected of having cancer, comprising steps of: contacting the sample with a substance of claim 1 that specifically binds to the 121P1F1-related protein or polynucleotide, respectively; and, determining that there is a complex of the substance and 121P1F1-related protein or the substance and 121P1F1-related polynucleotide, respectively.
 71. An assay of claim 70 for detecting the presence of a 121P1F1-related protein in a biological sample from a patient who has or who is suspected of having cancer, comprising steps of: contacting the sample with an antibody or fragment thereof either of which specifically bind to the 121P1F1-related protein; and, determining that there is a complex of the antibody or fragment thereof and 121P1F1-related protein.
 72. The assay in accordance with claim 70 further comprising a step of: taking a sample from a patient who has or who is suspected of having cancer.
 73. The assay of claim 70 for detecting the presence of an 121P1F1 polynucleotide in a biological sample, comprising: contacting the sample with a polynucleotide probe that specifically hybridizes to the polynucleotide of FIG. 2A, FIG. 2B, FIG. 2C, FIG. 2D, FIG. 2E, or FIG. 2F (SEQ ID NO: ______); and, detecting the presence of a hybridization complex formed by the hybridization of the probe with 121P1F1 polynucleotide in the sample, wherein the presence of the hybridization complex indicates the presence of 121P1F1 polynucleotide within the sample.
 74. An assay in accordance with claim 70 for detecting the presence of 121P1F1 mRNA in a biological sample from a patient who has or who is suspected of having cancer, said method comprising: producing cDNA from the sample by reverse transcription using at least one primer; amplifying the cDNA so produced using 121P1F1 polynucleotides as sense and antisense primers, wherein the 121P1F1 polynucleotides used as the sense and antisense primers are capable of amplifying 121P1F1 cDNA; and detecting the presence of the amplified 121P1F1 cDNA.
 75. A method for monitoring 121P1F1 gene products in a biological sample from a patient who has or who is suspected of having cancer, the method comprising: determining the status of 121P1F1 gene products expressed by cells in a tissue sample from an individual; comparing the status so determined to the status of 121P1F1 gene products in a corresponding normal sample; and, identifying the presence of aberrant 121P1F1 gene products in the sample relative to the normal sample.
 76. A method of monitoring the presence of cancer in an individual comprising: performing the method of claim 75 whereby the presence of elevated gene products 121P1F1 mRNA or 121P1F1 protein in the test sample relative to the normal tissue sample indicates the presence or status of a cancer.
 77. The method of claim 76 wherein the cancer occurs in a tissue set forth in Table I. 